• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.819-824
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• 2007

The present study describes the cytotoxic effect of Artemisia fukudo extracts. The extract from A. fukudo by 80% ethanol was fractionated with n-hexane, dichloromethane, ethylacetate, and butanol in serial. The cytotoxicity of A. fukudo extracts was examined for its effect on the growth of HL-60 cells by the colorimetric 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, we used the HL-60 cells to see what effects the A. fukudo extracts will have on apoptosis of cancer cells. We checked the cell activity, cell morphologic changes, DNA fragmentation, and DNA content after 24 hr incubation with administering 25 ${\mu}g/mL$ of the A. fukudo extracts. In the treatment of the low concentration of n-hexane and dichloromethane fractions, the survival rate of HL-60 cells is lower than that of the control. The laddering-pattern DNA fragmentation was observed in the treatment with n-hexane and dichloromethane fractions. The DNA content of the cells apoptosis measured as the density of sub-$G_{1}$ hypodiploid cells by flow cytometric analysis. The number of sub-$G_{1}$ hypodiploid cells increased in the treatment with n-hexane and dichloromethane fractions. These fractions obstructed the cell cohesion and caused the blebbing of the cell membrane and fragmentation of the nucleus, both of which are symptoms of apoptosis. These results suggest that A. fukudo has a great potential value as food additives, medicinal supplements for patients with chronic diseases, and preventive measures against cancer.

• Journal of Korean Society of Forest Science
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• v.85 no.4
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• pp.565-570
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• 1996

The landslide survey in an area of about 10 ha was carried out in Hyuseok-dong. Youngchoon-myeon, Danyang-gun, Choongcheongbuk-do. The results showed that there were many indications on geographical changes, such as scars, depressions, upheavals, cracks and destructions of the construction features. The mean degree of slope of the study area was $28^{\circ}$. The results of surface displacement in this area showed that landslide mass is moving in the direction of SE~SEE, and horizontal and vertical movement rate of the center of moving mass was about 1.2m/year and 0.86m/year, respectively. It was estimated that the amount of the soil erosion in the cliff at the lower edge of this area was 0.823m due to the caving erosion by the Namhangang(River) from June through November, 1995.

• Journal of Korean Society of Forest Science
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• v.98 no.3
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• pp.242-246
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• 2009

We examined sapflow of two populus tree species (Populus alba ${\times}$ Populus glandulosa and Populus euramericana) grown under Swine wastewater treatment, and measured relationship between sapflow and climate facters. Sapflow of P. euramericana was 1.5 times greater than that of P. alba ${\times}$ P. glandulosa from September to late December 2007. However, sapflow of P. euramericana and P. alba ${\times}$ P. glandulosa was similar after late January. The relationship between the mean temperature and sapflow was relatively high. There was a decrease in sapflow tendency of the two poplar species during the late October when the mean temperature was low. Trends on relationship between the relative humidity and sapflow showed low relationship. However, data showed weak relationship between sapflow and precipitation. During the period of less precipitation, sapflow of P. alba ${\times}$ P. glandulosa has no relationship. Sapflow of two populus species was still active during non-growing season. P. euramericana was considered as a better species than P. alba ${\times}$ P. glandulosa, in terms of phytoremediation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.93-97
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• 2007

Even in the moderately concentrated anionic surfactant system, some special encapsulation method can shield the papain enzyme from proteolytic attacks. The stabilization of enzyme has been a major issue for successful therapies. In this study, we first stabilized an enzyme, papain in the microcapsules by using polyols, polyethyleneglycol (PEG), poly-propyleneglycol (PPG), and PEG-PPG-PEG block copolymer. In the analysis of EDS and CLSM, it was demonstrated that polyols are effectively located in the interface of papain and polymer. Polyols located in the interface had an ability to buffer the external triggers by hydrophobic partitioning, preventing consequently the catalytic activity of papain in the micro-capsules. Second. we introduced multi-layer capsulation methods containing ion complex. Such a moderately concentrated anionic surfactant system as wash-off cleansers, surfactants and waters can cause instability of entrapped enzymes. Surfactants and water in our final products swell the surface of enzyme capsules and penetrate into the core so easily that we can not achieve the effect of enzyme, papain. In this case, the ion complex multi-layer capsule composed of sodium lauroyl sarcosinate and polyquaternium-6 could effectively prevent water from penetration into the core enzyme, followed by in vivo test, and evaluate the stratum corneum (SC) turn-over speed.

• Journal of Ginseng Research
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• v.23 no.4
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• pp.211-216
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• 1999

The red ginseng marc was roasted at various conditions and its organo-leptic properties and flavor components were investigated. Scorched-rice odor and roasted odor and the roasted red ginseng marc powder were much stronger than those from the unroasted red ginseng marc powder while earth odor, old-rice odor, metalic off-odor and woody odor were much weaker than those from the unroasted red ginseng marc. Savory odor and scorched-rice odor as well as savory taste and astringent taste from the water-extract of red ginseng marc roasted at $200^{\circ}C$ were stronger, but smoky odor, woody odor, biner taste and rough taste were weaker than those roasted at $230^{\circ}C$. Seven kinds of pyrazines, two kinds of carbonyl compounds, seven kinds of acids, two kinds of esters, five kinds of phenolic compowlds, maltol and six other compounds were identified as flavor components in distillate from red ginseng marc roasted at $200^{\circ}C$ for twenty minuties. Of them pyrazines and maltol are thought to be compounds which have characteristic odor such as roasted odor and/or scorchedrice odor in the roasted red ginseng marc.

• Korean journal of applied entomology
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• v.59 no.2
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• pp.117-127
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• 2020

We monitored on the hitchhiker insect pests from a total of 112 foreign vessels entering into Korea during 109 days from 1. Jun. to 17. Sep. in 2018. As the results, a total of 336 samples of the hitchhiker insects were collected using simply collecting method by hands, one of interception method and identified as 159 species of 47 families under nine orders on the basis of integrative species identification. Among them, 14 species of nine families under three orders were detected as undistributed species in South Korea. We reported on the 14 undistributed species in South Korea with the data sheets for the risk assessments on invasive or invasive likelihood species. Also, we suggested and used the term 'not-distributed species' for avoiding confusion with 'unrecorded species', 'unreported species', or 'new reported species'.

• Journal of Korea Foresty Energy
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• v.23 no.2
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• pp.21-28
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• 2004

Three different strains of Lentinula edodes, Sanlim 5-Ho, Sanlim 6-Ho and Nongki 3-Ho, were cultured in the sawdust media of Mongolian oak(Quercu mongolica Fisch) for 90 days under dark and light conditions(each 30 days) and fruiting period(30 days). Weight loss of sawdust media was determined after fungal cultures and the contents of ergosterol in fungal mycelia were quantified by HPLC analysis followed by solvent extraction. Compared with the two other fungal strains$(8\%)$, weight loss of Sanlim 5-Ho was slightly lowered to $7\%$. The level of ergosterol content, a parameter for fungal growth, was continuously enhanced in Sanlim 5-Ho for dark and light incubation periods. However, Sanlim 6-Ho and Nongki 3-Ho recorded the maximized fungal growth under light condition. In fruiting periods the ergosterol contents were lowered in the three strains. Intra- and extracellular enzymes during cultural and fruiting periods were also characterized. The activity of Mn-peroxidase and laccase, which are characteristics enzymes for white rot fungi as lignin degrading enzymes, were determined as a high level overall the periods. As cellulose degrading indicators, the activity of CMCase, avicelase, xylanase and glucanase were detectable in initial incubation period.

• Journal of Korea Foresty Energy
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• v.23 no.2
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• pp.34-43
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• 2004

This study was examined to investigate the effect of wood carbonization products on growth and body composition of oliver flounder(Paralichthys olivaceus) as a part of fishery utilization of charcoal and pyroligneous acid. Weight gain in oliver flounder fed with wood carbonization products was higher than that of the control group. Feed efficiency between the groups fed with charcoal and Mogchotan(the mixture of charcoal and pyroligneous acid, 80:20, w/w) and the control group showed no significant difference(p>0.05) and was significantly higher in group fed with pyroligneous acid than the control group(p<0.05). All groups fed with wood carbonization products were also higher than the control group in hemoglobin and hematocrit. Especially, the amount of both components was significantly higher in group fed with Mogchotan(p<0.05). The result indicates that the addition of Mogchotan can improve the health of oliver flounder, and was excellent in the mortality and the recovery rate. In conclusion, Mogchotan treatment would be suitable way for improving the growth and health of oliver flounder.

• Journal of Korea Foresty Energy
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• v.23 no.2
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• pp.9-20
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• 2004

Growth and nutrients of Xinjiang poplar (Populus alba var. pyramidalis) seedlings treated with fertilizers and soil amendments were examined in nursery and windbreak at Dengkou after 6 months of treatments in Inner Mongolia, China. Soil water content was $10\%$ higher than that at most soils in desert. Height and diameter growth and foliar nitrogen (N) concentration were higher at N and nitrogen plus phosphorus (N+P) fertilizer treatments than other treatments. However, there were no differences in foliar P concentration among treatments in windbreak and foliar P concentration was higher at control than at other treatments in nursery. Yuho treatment increased height and diameter growth. Nitrogen appeared an influenciug factor for early growth of Xinjiang poplar, and P had a minor affect on growth. More long-time studies were needed to elucidate the effects of fertilizers and soil amendments on growth and nutrients of Xinjiang poplar.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.114-120
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• 1997

Frequencies and the types of abnormal vertebrae in the juvenile of five cyprinid fishes collected from the Kumho River during 1995 and 1996 were examined. Types of vertebral anomalies in investigated species were spinal curvature, fused vertebrae, helical sutures of cetera and abnormal vertebrae with one or two additional spines. The frequencies and the types of vertebral anomalies were different among the species. Of all the examined species, the type with one or two additional spines showed the highest frequencies, 11.72~12.11%. The frequencies of fused vertebrae was 4.45~7.68%. Thes two types of vertebral anomalies were observed in all species. Among the several types of fused vertebrae, the frequencies of double fused vertebrae were higher than those of other types. Also, the incidence of fused vertebrae located in the caudal region of vertebral column was much higher than that in other regions. The percentages of spinal curvature and helical sutures of vertebrae in the investigated species were 0.02~0.15% and 0.02%, respectively. Among the examined specimens, vertebral anomalies include fused vertebrae and one or two additional spines were shown in the three species, Korean slender gudgeon (Squalidus gracilis majimae), False dace (Pseudorasbora parva) and Crucian carp (Carassius auratus). In addition to the two vertebral anomalies, spinal curvature was shown in the Korean gudgeon (Squalidus chankaensis tsuchigae). Dark chub (Zacco temmincki) had fused vertebrae, one or two additional spines, spinal curvature, and helical sutures of vertebrae. This species has the most variable vertebral anomalies. Frequencies of fused vertebrae and one or two additional spines in the all tested fishes were not related with their standard lengths measured. However, spinal curvature and helical sutures of vertebrae were shown only in the specimens smaller than 20mm in standard length.