• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.550-555
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• 2001

Insulin-like growth factor-I (IGF-I) is a mitogenic peptide with a molecular mass of 7 kDa. It is produced mainly in the liver and has important functions in the regulation of development and somatic growth. Moreover, Serum IGF-I concentration is regulated by the quantity and the nutritional quality of dietary protein. To determine the IGF-I level in Korean rockfish, Sabastes schlegeli, were fed four experiment diets that contained different protein quantities, namely $30\%,\;40\%,\;50\%\;and\;60\%$ for 70 days. Weight gain of the fish increased depending dietary protein quantity, Also, IGF-I concentrations increased according to dietary protein quantity, Feeding experiments were conducted to examine the effects of dietary protein sources on the serum IGF-I level in Korean rockfish, Fish meal (CO), soybean meal (SM), corn-gluten meal (CGM), meat meal (MM) and feather meal (FM) were used as variable protein sources of the formulated diet. IGF-I concentrations of the CO and MM groups ($277.7\pm23.2,\;291.5\pm41.2\;ng/mL$) were higher than those of the CGM and FM groups ($208.9\pm21.3,\;217.2\pm38.2\;ng/mL$). And IGFBP-3 levels by western blot analysis increased in good protein diets such as in the CO and MM groups. In conclusion, IGF-I may be a sensitive indicator the protein metabolism in fish as well as mammalian.

• Transactions of Materials Processing
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• v.18 no.2
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• pp.166-171
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• 2009

In this paper, a basic study on micro cutting process with SM20C at low temperature environment was performed. In macro cutting fields, the cryogenic cutting process has been applied to cut the refractory metal but, the serious problem may be generated in micro cutting fields by the cryogenic environment. However, if the proper low temperature is applied to micro cutting area, the cooling effect of cutting heat is expected. Such effect can make the reduction of tool wear and burr formation. For verifying this possibility, the micro cutting experiment at low temperature was performed and SEM images were analyzed.

• 한국정보디스플레이학회:학술대회논문집
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• 2006.08a
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• pp.268-269
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• 2006

The estimation of applicability $SrTiO_3:Pr^{3+}$, Al to a class low-voltage flat-panel displays based on field emission effect, which have average value anode voltage $U_a=300V$ and current density $j=100\;mA/sm^2$ at duty 240, has given positive result. In the same time observably for the most effective sample in similar conditions of excitation high brightness - more than $500\;Kd/m^2$ and linear brightness dependences from current density and voltage allows to decrease greatly the power which consumed by the device.

• 한국초전도학회:학술대회논문집
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• 2007.07a
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• pp.6-6
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• 2007

• The Journal of the Acoustical Society of Korea
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• v.12 no.2
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• pp.5-13
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• 1993

The purpose of this paper is to suggest and analyze the parallel algorithm for merging two heaps, on SIMD-SM-R parallel computer. In order to create the parallel algorithm for merging two heaps, we have classified two subproblems. For the first method, to select node p as a LEVEL-FIND function, Wyllie(19) suggests the method with time complexity O(log n) while this thesis has O(log(n/k)). For the second method, to merge two subheap, our algorithm has O(log(n/k)*log(n)) using max(2**(i-1), 「(m+1)/4」)'s processors while Dekel and Sahni(4)'s method and Hong's method(18) have O(log m). Also our parallel algorithm's EPU is close to 1 and so has an optimal speed-up ratio.

• Journal of Nutrition and Health
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• v.53 no.6
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• pp.563-569
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• 2020

Purpose: The vascular smooth muscle cells (VSMCs) in mature animals have implicated to play a major role in the progression of cardiovascular diseases such as atherosclerosis. This study aimed at optimizing the protocol in culturing primary VSMCs (pVSMCs) from rat thoracic aorta and investigating the effect of cellular zinc (Zn) deficiency on cell proliferation of the isolated pVSMCs. Methods: The thoracic aorta from 7-month-old Sprague Dawley rats was isolated, minced and digested by the enzymatic process of collagenase I and elastase, and then inoculated with the culture Dulbecco Modified Eagle Medium (DMEM) at 37℃ in an incubator. The primary cell culture morphology was observed using phase-contrast microscopy and cellular Zn was depleted using Chelex-100 resin (extracellular zinc depletion only) or 3 µM N,N,N',N'-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN) (extracellular and intracellular zinc depletion). Western blot analysis was used for the detection of SM22α and calponin as smooth muscle cell marker proteins and von Willebrand factor as endothelial cell marker protein to detect the culture purity. Cell proliferation by Zn depletion (1 day) was measured by MTT assay. Results: A primary culture protocol for pVSMCs from rat thoracic aorta was developed and optimized. Isolated cultures exhibited hill and valley morphology as the major characteristics of pVSMCs and expressed the smooth muscle cell protein markers, SM22α and calponin, while the endothelial marker von Willebrand factor was hardly detected. Zn deprivation for 1 day culture decreased rat primary vascular smooth muscle cell proliferation and this pattern was more prominent under severe Zn depletion (3 µM TPEN), while less prominent under mild Zn depletion (Chelexing). Conclusion: Our results suggest that cellular Zn deprivation decreased pVSMC proliferation and this may be involved in phenotypic modulation of pVSMC in the aorta.

• Analytical Science and Technology
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• v.25 no.1
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• pp.83-90
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• 2012

The objective of the study was to estimate the measurement uncertainty associated with determination of creatinine (Cr) in urine samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Centrifuged urine samples (10 ${\mu}L$) were diluted with 390 ${\mu}L$ of distilled water. To 20 ${\mu}L$ aliquots of diluted urine samples, 30 ${\mu}L$ of internal standard solution (Cr-$d_3$, 5 ${\mu}g/mL$) and 10 ${\mu}L$ of acetonitrile were added and filtered. The samples (1 ${\mu}L$) were introduced into LC-MS/MS with no further pretreatment. Cr was separated on a multi-mode ODS column (Scherzo SM-C18, 75 ${\times}$ 2.0 mm I.D., 3 ${\mu}m$) and quantified by LC-MS/MS operating in MRM mode (Cr, m/z 114.0${\rightarrow}$ 86.0; Cr-$d_3$, m/z 117.0${\rightarrow}$ 89.1). The four factors that contribute uncertainty to the final result were extracted and evaluated. The principal factors of contribution to combined standard uncertainty were sample dilution, calibration curve and repeatability, while the preparation of standard solution was only a minor factor. Relative extended uncertainty of the measured concentration was 14.2% in a real urine sample.

• Journal of Korean Society of Steel Construction
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• v.22 no.4
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• pp.389-398
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• 2010

The flexural strength of composite HSB I-girders under a positive moment was investigated using the moment-curvature analysis method to evaluate the applicability of the current AASHTO LRFD design specifications to such girders. A total of 2,391 composite I-girder sections that satisfied the section proportion limits of the AASHTO LRFD specifications was generated by the random sampling technique to consider a wide range of section properties. The flexural capacities of the sections were calculated inthe nonlinear moment-curvature analysis in which the HSB600 and HSB800 steels were modeled as an elasto-plastic strain-hardening material, and the concrete, as a CEB-FIP model. The effects of the ductility ratio and the compressive strength of the concrete slab on the flexural strength of the composite girders made of HSB and SM520-TMC steels were analyzed. The numerical results indicated that the current AASHTO LRFD equation can be used to calculate the flexural strength of composite girders made of HSB600 steel. In contrast, the current AASHTO LRFD equation was found to be non-conservative in its prediction of the flexural strength of composite HSB800 girders. Based on the numerical results of this study for 2,391 girders, a new design equation for the flexural strength of composite HSB800 girders in a positive moment was proposed.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.2
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• pp.196-203
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• 2003

The application of sealants is a highly technique-sensitive procedure, requiring an extremely dry field prior to placement. Moisture contamination of the etched enamel surface before sealant placement is cited as the main reason for sealant failure. The purpose of this study was to evaluate the effects of different methods of sealant application on the shear bond strength of sealants to enamel. In groups 1, 2, 3, 4 Teethmate(unfilled sealant) was used, while Ultraseal XTplus(filled sealant) was used in groups 5, 6, 7, 8. Groups 1 and 5(control) were acid etched for 15 seconds using 35% phosphoric acid, washed and then dried. In groups 2, 6 drying agents were applied, and in groups 3, 7 bonding agents were applied and light cured. In groups 4 and 8 both drying agent and bonding agent were applied. Then sealant was cured to the specimen using molds 3mm in diameter and 2mm in height. Thermocycling was performed and shear bond strength was finally measured. The following results were obtained : 1. Groups using filled sealant(groups 5, 6, 7, 8) showed higher shear bond strengths compared to groups using unfilled sealant(groups 1, 2, 3, 4). 2. Among groups using unfilled sealant(groups 1, 2, 3, 4), groups 2, 3, 4 showed significantly higher shear bond strength compared to group 1(p<0.05). There were no significant differences among groups 2, 3 and 4. 3. There were no significant differences(p>0.05) among groups using filled sealant(groups 5, 6, 7, 8). 4. When modes of fracture were examined, cohesive failure was observed in groups 2, 3 and 4.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.4 no.4
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• pp.42-49
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• 1995

It is not clearly known how defects or inclusions of a low carbon steel affect a fatigue strength. We study this issue using SM15C materials. The investigation is carried out by a quantitative evaluation, and experimental findings are: (1) a fatigue limit of A series smooth specimen is 205MPa, and that of B, C, D series is 245MPa, 304MPa and 245MPa, respectively. (2) the fatigue limit varies with respects to the stress distribution I the vicinity of a defects and crack. (3) the micro hole creates a half-circular shape crack, while the hole depth is not critical to the fatigue strength, (4) considering the fatigue strength, the hole diameter is more significant than the hole depth, and (5) Fatigue limit of artificially defected specimen is lower than that of a flawless one (5-10%), however, there exist allowance size and depth of defect which don't get to influence at fatigue limit.