• Applied Biological Chemistry
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• 제22권1호
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• pp.10-17
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• 1979

인삼엽(人蔘葉)을 ether, benzene-butanol 혼합용매등으로 색소류(色素類)기타 지용성물질(脂溶性物質)등을 제거하고, methanol과 n-butanol로 연속추출하여 n-butanol soluble saponin을 얻고 계속해서 liquid chromotography 를 행하여 분획별(身劃別)로 인삼엽(人蔘葉) saponin을 분리 정제하였고, 그 화학적(化學的)및 분자구조학적(分子構造學的) 특성(特性)을 인삼근(人蔘根) saponin과 비교 검토한 결과는 다음과 같다. 1. 인삼근(人蔘根)과 엽(葉) saponin의 2차원(차원) TLC pattern은 다소(多少) 차이(差異)차가 있으나, 다수(多數)의 spot들이 일치(一致)하였다. 인삼엽(人蔘葉)에는 ginsenoside Rd가 존재하지 않고, ginsenoside Rc는 엽(葉)에, ginsenoside $Re_1$ 및 $Rg_1$는 근부(根部)에 더 많이 분포(分布)되어 있었다. 2. 인삼엽(人蔘葉)에는 인삼근(人蔘根)에 비하여, panaxadiol 계(系) saponin이 panaxatriol 계(系) saponin보다 더높은 함량을 보였다. 3. n-butanol soluble saponin으로 liquid chromatography를 행하여 인삼근(人蔘根)에서는 Fr. 1.과 Fr. 2.를 얻었고, 인삼엽(人襲葉)에서는 Fr. 1, Fr. 2. 및 Fr. 3.의 분획(分劃)을 얻을 수 있었다. 인삼근(人蔘根)의 Fr. 1 및 엽(葉)의 Fr. 1.과 Fr. 2.등 3매의 분획(分劃)은 saponin test반응(反應)에 양성(陽性)을 보이고, 그 IR spectra는 동일(同一)한 것으로 나타났다.

• 한국식품영양과학회지
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• 제30권1호
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• pp.1-5
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• 2001

This study was carried out to isolate saponin compounds from red-ginseng efflux, which was produced during the industrial processing of red-ginseng from fresh ginseng. We isolated crude saponin from the efflux extract (moisture content 35.0%) by using Diaion HP-20 adsorption method. Non-saponin fraction, which was adsorbed on Diaion HP-20 resin, was removed by eluating with $H_{2}O$ and 25% spirit. Then crude saponin was eluated with 95% spirit, continuously. Saponin in the eluated fractions was confirmed by TLC analysis. Crude saponin isolated from red ginseng efflux extract contained 12.10% of saponin. whereas those of white ginseng and red-ginseng were 3.30 and 3.39%, respectively. Ginsenoside contents showed the highest contents kin crude saponin from red ginseng efflux extract. Expacilly, the ginsenoside-$Rb_{1}$ and Re showed the highest contents in red-ginseng efflux extract when compared with those of white ginseng and red ginseng crude saponins. And the other ginsenosides except ginsenoside-$Rb_{1}$ and -Re also showed the highest contents in red ginseng efflux extract. However, the ratio of PD saponin (Panaxadiol saponin: $Rb_{1}+Rb_{2}$+Rc+Rd) to PT saponin (panaxatriol: $Re+Rg_{1}$) showed almost the same level when compared with those of ginseng saponin fractions. Ratio of PD/PT from red ginseng efflux extract was 1.99. Ratios of PD/PT from white ginseng and red ginseng were 1.85 and 1.84, respectively. Saponin purity, which was calculated by ratio percent of total ginsenoside to curde saponin content, was 45.90%. In case of white ginseng and red ginseng, the purities were 35.50 and 36.00%, respectively. However, by PHLC analysis, we confirmed that crude saponin isolated from red ginsengs. It suggested that crude saponin isolated from red ginseng ellux also would be useful component as ginseng saponins.

• Journal of Ginseng Research
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• 제30권1호
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• pp.1-7
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• 2006

Ceramide has been involved in celt death and acted as a lipid mediator of stress responses. Elevation of ceramide level was reported to occur in oxidative stress and lead to cell death in many cell types. This study was undertaken to elucidate a protective role of ginsenoside Rgl in cell death induced by oxidative stress. When LLC-PK1 cells were treated with $H_2O_2$ at a concentration of $400{\mu}M$ for 5 hr, cell death was observed and a released LDH activity indicative of cytotoxicity was Increased. $H_2O_2$ exposure to LLC-PK1 cells was shown to elevate the content of total ceramide by approximately 200% compared to control cells. Ceramide level was hypothesized to be a key to a reversal of cell death to survival. Ginsenoside Rgl at the concentrations ranging from 12.5 to $250{\mu}M$ protected LLC-PK1 cells from cell death induced by $H_2O_2\;at\;400{\mu}M$ for 5 hr, and decreased the ceramide level relative to $H_2O_2$. Ginsenoside Rgl inhibited neutral human ceramidase by 71% of controls, while sphingomyelinase was not inhibited. These results suggest that ginsenoside Rgl show the protection against cell death via the modulation of ceramide metabolism, and ceramide may be a promising therapeutic target for human diseases related to cell death.

• Natural Product Sciences
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• 제22권2호
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• pp.93-101
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• 2016

For efficient quality control of the Samryeongbaekchul-san decoction, a powerful and accurate an ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was developed for quantitative analysis of the thirteen constituents: allantoin (1), spinosin (2), liquiritin (3), ginsenoside Rg1 (4), liquiritigenin (5), platycodin D2 (6), platycodin D (7), ginsenoside Rb1 (8), glycyrrhizin (9), 6-gingerol (10), atractylenolide III (11), atractylenolide II (12), and atractylenolide I (13). Separation of the compounds 1 - 13 was performed on a UPLC BEH $C_{18}$ column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ with a gradient solvent system of 0.1% (v/v) formic acid aqueous-acetonitrile. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$. Calibration curves of all compounds were showed good linearity with values of the correlation coefficient ${\geq}0.9920$ within the test ranges. The values of limits of detection and quantification for all analytes were 0.04 - 4.53 ng/mL and 0.13 - 13.60 ng/mL. The result of an experiment, compounds 2, 6, 12, and 13 were not detected while compounds 1, 3 - 5, and 7 - 11 were detected with 1,570.42, 5,239.85, 299.35, 318.88, 562.27, 340.87, 12,253.69, 73.80, and $115.01{\mu}g/g$, respectively.

• Journal of Electrical Engineering and Technology
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• 제11권6호
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• pp.1754-1762
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• 2016

Finite difference analysis were applied to study the principle operation of monopole antenna for moisture determination in oil palm fruit at 2 GHz. The electromagnetic field interact with oil palm fruit on the interface between the antenna and oil palm fruit and cause a reflection. The reflection measurement is based on mismatch impedance or dielectric properties between two media. Reflection coefficient is used to quantify the level of reflection. The monopole antenna was made of RG405/U semi-rigid coaxial cable with an inner and outer diameter of 0.45 mm and 1.50 mm, respectively with 2.23 mm length of protruding conductor over 5.66 cm length of monopole antenna. This monopole antenna for moisture detection was compared with induced EMF method in terms of reflection coefficient at 2 GHz. The results show that the complex reflection coefficient measured using monopole antenna provides significant results to predict moisture content in oil palm fruit.

• 한국의류학회지
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• 제16권1호
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• pp.73-83
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• 1992

This paper examined risk reduction methods utilized by consumers in apparel buying situation in multidimensional conceptual framework, and analyzed the relationships between risk types, consumer demographic variables and preferences of risk reduction methods. Samples of 224 consumers were deliberately selected to include various demographic characteristics such as sex, age, educational level, occupation, income level. The results of the principal axis factor analysis indicated that 26 item risk reduction methods could be summarized into 6 meaningful factors; Marketer-dominated Information Sources Use (MIS), Prepurchase Deliberation / Observation i Dependence on Past Buying Experience (DOE), Independent Information Sources Use (lIS), Interpersonal Information Sources Use (PIS), Brand Loyalty (BL), Label Reading / Guarantee Buying (RG). DOE were used most whereas IIS used least. Correlations of various types of risk perceived with the preference of risk reduction methods were significant especially for positive relationship between psychologi-cal and/or economic risk and DOE, and between social risk and/or fashionability loss and MIS. Results of ANOVA and Duncan test suggested that sex, age, educational level, occupation of consumers can act as ones of determinant variables on making differences in the use of risk reduction methods.

• Journal of Mechanical Science and Technology
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• 제17권9호
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• pp.1380-1387
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• 2003

When a cold HPSI (High pressure Safety Injection) fluid associated with an overcooling transient, such as SGTR (Steam Generator Tube Rupture), MSLB (Main Steam Line Break) etc., enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena will arise due to incomplete mixing. If the stratified flow enters the downcomer of the reactor pressure vessel, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. As general thermal-hydraulic system analysis codes cannot properly predict the thermal stratification phenomena, RG 1.154 requires that a detailed thermal-mixing analysis of PTS (pressurized Thermal Shock) evaluation be performed. Also. previous PTS studies have assumed that the thermal stratification phenomena generated in the stagnated loop side of a partially stagnated primary coolant loop are neutralized in the vessel downcomer by the strong flow from the unstagnated loop. On the basis of these reasons, this paper focuses on the development of a 3-dimensional thermal-mixing analysis model using PHOENICS code which can be applied to both partial and total loop stagnated cases. In addition, this paper verifies the fact that, for partial loop stagnated cases, the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is almost neutralized by the strong flow of the unstagnated loop but is not fully eliminated.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2006년도 정기 학술대회 논문집
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• pp.1025-1032
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• 2006

The PVRC(Pressure Vessel Research Council) damping is for the response spectrum analysis of the piping system. In this study, the possibility to apply it to the time history analysis is evaluated to reduce the higher conservatism for the structural integrity. The evaluation was performed for the surge line connecting the pressurizer to the hot-leg, and the whole mode includes the RCS and the building structures with the surge line. The analyses were performed using ANSYS code. The first modal analysis shows the modes of the surge line are isolated from those of the other structures. The composite modal damping was calculated with PVRC damping for the surge line and RG 1.60 damping for the other structures by using ANSYS routines. Of the calculated composite modal damping values, the composite modal damping values related to the modes of the surge line were replaced with the PVRC damping values with respect to its frequencies. With this replacement, the composite modal damping values of the other structures were not changed. Based on this decouple characteristics, the time history analyses for the seismic events with PVRC damping for the surge line were performed. And the results show the resultant loads can be reduced by up to 50%.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2001년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2001
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• pp.67-74
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• 2001

This paper presents the Safe Shutdown Earthquake(SSE) input motion for the seismic design of the Advanced Power Reactor 1400(APR1400). The Design Ground Response Spectra(DGRS) far the SSE is based on the design spectrum specified in regulatory Guide(RG) 1.60 of U.S. Nuclear Regulatory Commission(US NRC), anchored to a Peak Ground Acceleration(PGA) of 0.3g and enriched in the high frequency range. This SSE seismic input motion is to be applied to the seismic analysis as the free-field seismic motion at the ground surface of both the rock and generic soil sites fur APRI1400. The enrichment for APR1400 seismic input motion is performed considering the current US NRC regulations, the seismic hazard studies performed by the Lawrence Livermore National Laboratory (LINL) and Electric Power Research Institute(EPRI) for the Central and Eastern United States nuclear power plant sites, and the seismic input motions used in the design certifications of the three existing U.S. advanced standard plants. It is represented by a set of DGRS and the accompanying Target Power Spectral Density(PSD) Function in both the horizontal and vertical directions.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1993년도 학술대회지
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• pp.36-39
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• 1993

We reported earlier (Br. J. Pharmac. 82. 485 - 491. 1984) that ginsenosides from Panax ginseng CA. Meyer antagonized noradrenaline or prostaglandin $F_{2\alpha}-induced$ contractions of pulmonary and intrapulmonary arterial rings of rabbits. Because this effect resembled that of acetylcholine (ACh). we questioned whether these acitons were due to release of nitric oxide from vaseular endothelium. We therefore determined whether ginsenosides could vasodilate preconstricted lungs and also protect against free radical injury. which normally eliminates the vasodilator response to ACh(J. Appl. Physiol. 71. 821 - 825. 1991 J. We found that ginsenoside $Rg_1$ or a mixture of saponins could ,a) vasodilate perfused. $U_{46619}-preconstricted$ lungs. b) promote increased synthesis of nitric oxide by endothelial cells in culture and c) prevent the pulmonary edema often associated with free radical injury (Biochem. Biophys. Res. Comm. 189. 670 - 676. 1992). Thus, vasodilator and protective effects of ginsenosides against free radical injury may reflect enhanced synthesis and release of nitric oxide. These data suggest that ginsenosides may be useful in treatment of pulmonary and systemic hypertension. Aided by grants from the National Institutes of Health. Bethesda.