This studies were conducted to investigate the effect of holding time of broiler at slaughter house on chicken meat quality. Short holding time ($1{\sim}2hrs $) increased chicken meat quality compared to long holding time ($14{\sim}15 hrs$), which resulted in 14% higher grade $1^+$ chicken. In portioned meat, short holding time ($1{\sim}2 hrs$) resulted in 15% higher incidence of grade $1^+$ chicken breast. Also, long holding time occurred higher PSE incidence of chicken breast. pH value of the chicken meat with short holding time was similar to that with long holding time, and that with medium holding time showed the lowest. $L^*$ (lightness) value of the chicken meat was lower with medium holding time than with short holding time, but that in muscle and skin showed increasing tendency with long holding time. Holding time showed no effect on $a^*$ (redness) of the chicken meat, but longer holding time decreased $b^*$ (yellowness) value. This indicated that long holding time adversely affected chicken meat quality.
An experiment was conducted at the greenhouse of the International Rice Research Institute in 1987 to find out the character of dry matter production, the potential seed production ability and the ecological and physiological strategies to adaptation. For these, two rice cultivars, IR64(lowland rice) and UPLRi-5(upland rice), and seven weed species were used ; Echinochloa glabrescens Munro ex Hook. f., E. cress-galls ssp. hispidula (Retz.) Honda, E. colons (L.) Link, Monochoria vaginalis (Burin. f.), Ludwigia octovalvis Jacq.) Raven, Fimbristylis miliacea Vahl and Cyperus difformis L. Adaptation strategies of weed species varied by species. However, they had efficient seed production strategy through different ways. In general, sedge weed species (F. miliacea and C. difformis) produced great amount of seeds at the expense of seed size through greater ratooning ability and low relative dry weight for flowering. For broadleaved weed species, greater number of descendants were obtained through high plasticity and low relative dry weight for flowering (M. vaginalis) or greater growth ability through effective photosynthetic efficiency (L. octovalvis). Grass weed species, on the other hand, produced their seeds through effective growth (net assimilation rate and relative growth rate), high ratooning ability (except E. crus-galls ssp. hispidula) or low relative dry weight to maximum dry weight for flowering (E. glabrescens). The harvest indices of the weed species were considerably lower than those of rice. Fimbristylis miliacea had the greatest ratooning ability followed by C. difformis, E. colons and E. glabrescens. The greatest seed productivity was recorded by C. difformis (279, 000) and L. octovalvis (268,000) while rice produced the least number of seeds (1300-6100). Log seed weight had a negative linear relationship with log seed number (y=6.30-1.48X, $R=-0.965^{**}$). For all species plant plasticity response was not directly correlated with mortality response.
Jin H. J.;Kim I. C.;Wee M. S.;Yeon S. H.;Kim C. D.;Cho C. Y.;Choi S. H.;Cho S. R.;Son D. S.;Kim Y. K.;Jung J. H.;Choi H. S.;Park C. K.
Journal of Embryo Transfer
/
v.20
no.3
/
pp.289-295
/
2005
This study was performed to investigate the relationship between Heat shock protein 70 (HSP70) gene polymorphism and in vitro fertilization(IVF) embryo development in the pigs. The single strand conformation polymorphism(SSCP) genotypes from HSP70 K1, K3 and K4 PCR products were detected different patterns. In cleavage rate of oocyte fertilized in vitro, HSP70 K1-AA genotype($73.1\%$) and K1-AB genotype($62.3\%$) showed significantly higher oocyte cleavage rate than HSP70 K1-BB genotype($49.3\%$)(p<0.05). And HSP70 K3-AA genotype ($72.4\%$) and K3-AB($62.2\%$) also showed significantly higher oocyte cleavage rate than HSP70 K3-BB genotype($49.1\%$)(p<0.05). The IVF embryo development of 2-cell stage according to HSP70 genotypes of sperm and pig breeds also showed a significant difference. The number of embryos developed to 2-cell stage in Landrace(28.8) and Duroc(29.8) were significantly higher than in Yorkshire(10.9)(p<0.05). And also HSP70 K4-AB genotype group(29.6) higher than HSP70 K4-AA genotype group(10.6)(p<0.05). However, the number of embryos developed to blastocyst stage did not showed significant differences among breeds as well as HSP70 genotypes. These resrults suggest that in vitro development in porcine early embryos may be affected by HSP70 genotypes and breeds.
The experiments were carried out to develop simulation model for estimating the yield of soybean in upland and paddy field condition. Field experiments were done at National Institute of Crop Science in 2005. The evaluated soybean cultivars were Taekwangkong, Daewonkong, and Hwangkeumkong. Soybean seeds were planted by hill seeding with 3-4 seeds and row and hill spacing were $60{\times}10cm$ in upland and $60{\times}15cm$ in paddy field. Seeds were sown on row (without making ridge) and on the top of ridge in upland and paddy field, respectively. Field parameters were measured yield components ($plants/m^{2}$, pod no./plant, and 100-seed weight, seed yield and growth characteristics (stem length, leaf area at each stage, and dry weight of shoot) and after measuring they were compared the relationships with seed yield and yield components and seed yield and growth characteristics. Seed yield of soybean was affected by cultivars and planting density. Seed yield was higher in upland than paddy field due to the higher planting density in upland field. The upland soybeans generally had lower 100-seed weight than that of paddy field. Seed yield of soybean in a paddy field was greatest in Taekwangkong and followed by Daewonkong and Hwangkeumkong. The harvest index of taekwangkong and Hwanggumkong was higher in upland than paddy field, however, it was higher in paddy field than upland in Daewonkong. Seed yield was greatest in Daewonkong in both experimental fields. The greatest stem length was observed in taekwangkong and Hwanggumkong (R6) in late growth stage in paddy field. Dry weight of shoot and pod, pod number, stem length, and stem diameter were higher grown in paddy field than grown in upland. Crop growth rate (CGR) of cultivars was higher in paddy field after 8 WAS(weeks after sowing) and it was greatest at 13 WAS in Daewonkong among the cultivars. In upland field, CGR was greatest in Taekwangkong and then followed by Daewonkong and Hwanggumkong during 12 and 15 WAS. There was no significant relationships between 100-seed weight and seed yield in both experimental fields. A significant positive relationship was observed between seed number and seed yield. The correlation coefficients between leaf area and shoot dry weight were about 0.8 during the whole growth stage except 5 WAS and 4-5 WAS in paddy field and upland, respectively. This experiment was done just one year and drained paddy field condition was not satisfied drained condition successfully at 7th leaf age of soybean by the heavy rain, so we suggest that the excessive soil water reduced seed yield in paddy field and the weather condition should be considered for utilizing of these results.
Vitrification of oocytes has been applied recently fur pigs, but remains elusive. The purpose of this study is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine oocytes. When immature follicular oocytes frozen-thawed were cultured for in vitro maturation, maturation rates to metaphase-II stage were higher in oocytes with (25%) than without (15%) cumulus cells. After In vitro fertilization of oocytes frozen-thawed, the maturation rates were also significantly (P<0.05) higher in oocytes with (41%) that than without (17%) cumulus cells. However, the penetration rates were higher in oocytes without (19%) that than with (9%) cumulus. In another experiment, porcine oocytes matured in vitro were frozen and thawed for in vitro fertilization. The penetration rates were higher than in oocytes without (35%) that than with (26%) cumulus cells. However, the proportions of oocytes dead after in vitro fertilization were significantly (P<0.05) higher in oocytes with that than without cumulus cells. On the other hand, the rates of penetration and dead oocytes at 6 h after in vitro fertilization were not significant differences between oocytes with and without cumulus cells. However, the proportions of dead oocytes with (18%) and without (16%) cumulus cells were higher than in oocytes of control group (0%). These finding indicated the possible broader application for OPS, as they demonstrated that the maturation and fertilization in vitro by frozen-thawed oocytes may be accompained by cumulus cells and culture periods according to the requirements of the survival ability after freezing of mature and immature oocytes in pigs.
Mulberry plants (Morus alba L.) were grown in pots with the following different nitrogen sources: ammonium sulphate, urea, ammonium nitrate, sodium nitrate + ammonium nitrate ($NO_3:NH_4$=2:1), and sodium nitrate. The effects of the nitrogen sources on mulberry yields, nitrogen recovery, distribution of ions and cation-anion balance (C-A) along leaf sequence and growth stage were investigated. The results were as follows: 1. Leaf yields and nitrogen recovery decreased with increasing $NO_3$-N application rates. 2. Relative cation contents in leaves in the early growth stages showed the following pattern : Na < Mg < Ca < K. However, the order of Ca and K reversed in the later stages. The order of anion contents chifted from $SO_4$ < $NO_3$ < Cl < $H_2PO_4$ in the early stages to $NO_3$ < Cl < $SO_4$ < $H_2PO_4$ in the later stages. 3. Contents of K, $H_2PO_4$, $SO_4$, $NO_3$, T-N and the sum of anion contents (${\sum}A$) were higher in upper leaves whereas Ca, Mg, Cl, the sum of cation contents (${\sum}C$) and (C-A) were higher in lower leaves. 4. When $NO_3$ in leaves decreased, Cl and K as counter-cations increased and consequently Ca decreased. 5. The (C-A) in leaves varied with leaf sequence and growth stage from 700 to 900 me/kg D.M.
Nationwide weed survey of paddy rice field conducted in 1981 and 1971 was compared and determined major dominant weed community types distributed by province. Based on the similarity coefficients between 1971 and 1981 the floristic composition based on the degree of dominance was greatly dissimilar while kind of weed flora were not much differed. Degree of dominance were concentrated to perennial weeds in 1981 while these were at animal weeds in 1971. The moat important 10 weed species and their dominance in 1981 were Monochoria vaginalis Presl.(22.2%), Sagittaria pygmaea Miquel(17.5%), S. trifolia L.(9.0%), Poramogeton distinctus Benn.(9.0%), Cyperus serotinus Rottb.(8.5%), Rotala indica Koehne(6.0%), Aneilema japonica Kunth(4.4%), Lindernia procumbens Philcox(3.9%), Eleocharis kuroguwai Ohwi(3.4%) and Ludwigia proarrara Roxb(3.0%), respectively while these for 1971 were R. indica(34.5%), Eleocharia acicularis Roem, et Schult (11.9%), M. vaginalis(11.1%), Cyperua difformis L.(8.7%), Echinochloa crus-galli Beauv.(6.9%), L. procumbens(3.3%), P. distinctus(3.1%), A. Japonica (2.4%), E. kuroguwai(1.8%) and P. hydropiper(1.8%), respectively. Weed occurrence was also closely related with soil type, cropping pattern and cultural practices. Particularly, the occurrence of P. distincrus was negatively correlated with the degree of land utilization. Weed community types dafined by two-dimensional ordination analysis were 11 for Jeonnam province, 9 for Gyeoaggi, Gangweon, Chungbuk and Gyeongnam provinces, 8 for Jeonbuk and Gyeongbuk provinces, 7 for Chungnam province and 4 for Jeju province, respectively.
Purpose: The conventional gamma camera is not ideal for scintimammography because of its large detector size (${\sim}500mm$ in width) causing high cost and low image quality. We are developing a small gamma camera dedicated for breast imaging. Materials and Methods: The small gamma camera system consists of a NaI (T1) crystal ($60 mm{\times}60 mm{\times}6 mm$) coupled with a Hamamatsu R3941 Position Sensitive Photomultiplier Tube (PSPMT), a resister chain circuit, preamplifiers, nuclear instrument modules, an analog to digital converter and a personal computer for control and display. The PSPMT was read out using a standard resistive charge division which multiplexes the 34 cross wire anode channels into 4 signals ($X^+,\;X^-,\;Y^+,\;Y^-$). Those signals were individually amplified by four preamplifiers and then, shaped and amplified by amplifiers. The signals were discriminated ana digitized via triggering signal and used to localize the position of an event by applying the Anger logic. Results: The intrinsic sensitivity of the system was approximately 8,000 counts/sec/${\mu}Ci$. High quality flood and hole mask images were obtained. Breast phantom containing $2{\sim}7 mm$ diameter spheres was successfully imaged with a parallel hole collimator The image displayed accurate size and activity distribution over the imaging field of view Conclusion: We have succesfully developed a small gamma camera using NaI(T1)-PSPMT and nuclear Instrument modules. The small gamma camera developed in this study might improve the diagnostic accuracy of scintimammography by optimally imaging the breast.
Attenuation correction is important in producing quantitative positron emission tomography (PET) images. Conventionally, photon attenuation effects are corrected using transmission measurements performed before tracer administration. The pre-injection transmission measurement approach may require a time delay between transmission and emission scans for the tracer studies requiring a long uptake period, about 45 minutes for F-18 deoxyglucose study. The time delay will limit patient throughput and increase the likelihood of patient motion. A technique lot performing simultaneous transmission and emission scans (T+E method) after the tracer injection has been validated. The T+E method substracts the emission counts contaminating the transmission measurements to produce accurate attenuation correction coefficients. This method has been evaluated in experiments using a cylindrical phantom filled with background water (5750 cc) containing $0.4{\mu}Ci/cc$ of F-18 fluoride ion and one insert cylinder (276 cc) containing $4.3{\mu}Ci/cc$. GE $Advance^{TM}$ PET scanner and Ge-68 rotating pin sources for transmission scanning were used for this investigation. Post-injection transmission scan and emission scan were peformed alternatively over time. The error in emission images corrected using post-infection transmission scan to emission images corrected transmission scan was 2.6% at the concentration of $1.0{\mu}Ci/cc$. No obvious differences in image quality and noise were apparent between the two images. The attenuation correction can be accomplished with post-injection transmission measurement using rotating pin sources and this method can significantly shorten the time between transmission and omission scans and thereby reduce the likelihood of patient motion and increase scanning throughput in PET.
Purpose: The purpose of this study was to evaluate if short-term serum thyroglobulin (Tg) elevation after radioiodine administration can predict successful radioiodine remnant ablation (RRA) and whether comparable RRA effectiveness is exhibited between a group administered with recombinant human thyrotropin (rhTSH) and a group experiencing thyroid hormone withdrawal (THW), in preparation for RRA. Materials and Methods: A retrospective chart review was performed on 39 patients in the rhTSH group and 46 patients in the THW group. They were treated for differentiated thyroid carcinoma by total or near total thyroidectomy, and referred for RRA between 2003 and 2006 (the rhTSH group) and between January and June of 2006 (the THW group). They were assessed for serum Tg levels just before I-131 administration (TgD0), reassessed 9 days later (TgD9), and again 6-12 months later. Results: RRA was successful in 64 (37 from the THW group and 27 from the rhTSH group) of the total 85 patients. The success rates of RRA had no statistically significant differences between the two groups. In both groups, TgD9/TgD0 values were significantly higher in the RRA success group (the rhTSH group; P = 0.03, the THW group; P = 0.04). By combining cutoff values of TgD0 and TgD9/TgD0, the successful RRA value was determined to be 96.7% (29/30) with TgD0$\leq$5.28 ng/mL and TgD9/TgD0>4.37 in both groups (the rhTSH group; 100% (16/16), the THW group; 92.9% (13/14)). Using logistic multivariate analysis, only TgD0 was independently associated with successful RRA. Conclusion: We may predict successful ablation by evaluating short-term serum Tg elevation after I-131 administration for RRA, in both rhTSH and THW patients.
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