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Relationship between HSP70 Gene Polymorphisms and IVF Embryo Development in Pigs  

Jin H. J. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Kim I. C. (Swine Science Division, NLRI, RDA)
Wee M. S. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Yeon S. H. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Kim C. D. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Cho C. Y. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Choi S. H. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Cho S. R. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Son D. S. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Kim Y. K. (Animal Genetic Resources Station, National Livestock Research Institute, RDA)
Jung J. H. (Animal Science and Biotechnology, Seoul National University)
Choi H. S. (Course of Pet Beauty Fashion Design, Kimcheon College)
Park C. K. (College of Animal Resource Science, Kangwon National University)
Publication Information
Journal of Embryo Transfer / v.20, no.3, 2005 , pp. 289-295 More about this Journal
Abstract
This study was performed to investigate the relationship between Heat shock protein 70 (HSP70) gene polymorphism and in vitro fertilization(IVF) embryo development in the pigs. The single strand conformation polymorphism(SSCP) genotypes from HSP70 K1, K3 and K4 PCR products were detected different patterns. In cleavage rate of oocyte fertilized in vitro, HSP70 K1-AA genotype($73.1\%$) and K1-AB genotype($62.3\%$) showed significantly higher oocyte cleavage rate than HSP70 K1-BB genotype($49.3\%$)(p<0.05). And HSP70 K3-AA genotype ($72.4\%$) and K3-AB($62.2\%$) also showed significantly higher oocyte cleavage rate than HSP70 K3-BB genotype($49.1\%$)(p<0.05). The IVF embryo development of 2-cell stage according to HSP70 genotypes of sperm and pig breeds also showed a significant difference. The number of embryos developed to 2-cell stage in Landrace(28.8) and Duroc(29.8) were significantly higher than in Yorkshire(10.9)(p<0.05). And also HSP70 K4-AB genotype group(29.6) higher than HSP70 K4-AA genotype group(10.6)(p<0.05). However, the number of embryos developed to blastocyst stage did not showed significant differences among breeds as well as HSP70 genotypes. These resrults suggest that in vitro development in porcine early embryos may be affected by HSP70 genotypes and breeds.
Keywords
HSP70 gene; IVF embryo; PCR-SSCP; pig;
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