• 대한치과교정학회지
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• 제27권1호
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• pp.45-54
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• 1997

본 연구의 목적은 bionator를 사용한 II급 1류 부정교합 환자의 치료후 결과를 토대로 치료전에 치료후 결과를 예측할 수 있는 항목들을 알아봄으로써 성장하는 II급 1류 부정교합을 가진 환자들의 치료에 도움을 얻는데 있다. bionator를 사용한 앵글 II급 1류 부정교합 환자 48명의 치료후 두부방사선계측사진을 통해 치료결과가 양호한 군(1군)과 치료결과가 불량한 군(2군)으로 나눈 후 양군의 치료전 측모두부방사선계측사진의 비교분석을 통해 두군 사이에 차이를 보이는 계측항목들을 알아보았고, 판별분석을 통해 다음의 결과를 얻을 수 있었다. 1. 치료전 골격계측 항목으로는 ANB, facial convexity angle, AB to facial plane angle 등이, 치성계측 항목으로는 L1 to A-Pog, U1 to facial plane, L1 to facial plane 등이, 연조직 계측항목으로는 Ricketts esthetic line 상에서 상, 하순의 돌출도가 양군 사이에 유의한 차를 보였다(SAS t-test, p<0.05). 2. 판별분석을 통해 유의성있게 나타나는 항목의 순위를 본 결과 L1 to facial plane, 하순의 돌출도, ANB과 FMIA 등이 양군의 치료결과의 예측에 도움을 주는 것으로 나타났다. 3. 증감판별분석을 통해 서로 독립적이며 상관계수가 높은 3개의 변수 - L1 to facial plane, articular angle, ANB- 를 선택하였으며, 이를 토대로 판별식을 도출하였다.

• 구강회복응용과학지
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• 제19권3호
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• pp.169-183
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• 2003

According to our recent investigation that the increase in the occlusal vertical dimension made the appendage muscle strength got higher, the occlusal appliances were made by increasing the occlusal vertical dimension "from the centric relation" position of the mandible. In this experiment, the authors tried to study the change in the appendage muscle strength due to increase in occlusal vertical dimension from intercuspal position(ICP) of mandible with the same subjects and manner as the former experiment. For this study, ten male athletes in a mean age of 23 year who were joined the former study were selected. All the subjects had a complete or almost complete set of natural teeth and reported no subjective symptoms of temporomandibular disorders. Upper and lower casts were mounted on the semi-adjustable articulator at the intercuspal position and a point was marked on the attached gingival area between the right canine and the right 1st. premolar in each upper and lower cast. From the points, the occlusal vertical dimension was increased by 2mm, 3.5mm and 5mm, and then each 10 maxillary type occlusal splint at each 3-increased position were fabricated with heat curing clear acrylic resin. Including the intercuspal position, the 3 kinds of occlusal splints were placed on the subjects individually, and then isokinetic muscle strength on 7 parts of the human appendage which are shoulder, knee, ankle, wrist, forearm, elbow and hip was measured with the CYBEX 6000 SYSTEM (Lumex, NewYork, USA). The results were as follows: The highest mean value in muscular strength was shown at the position of 2mm-increased vertical dimension. The muscle strength during internal/external rotation of shoulder and knee, plantarflexion of ankle, flexion of elbow, and flexion and extension of hip at the increased occlusal vertical dimension position were significantly higher than them at the intercuspal position (p<0.05). Only in view of the increase in the appendage muscle strength, regardless of the way of making the occlusal splints by elevating the occlusal vertical dimension from the centric relation position or intercuspal position, the occlusal splints had an effect on the increase of isokinetic muscle strength at the occlusal vertical dimension which increased within the proper range on the habitual arc of closure.

• 구강회복응용과학지
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• 제24권4호
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• pp.361-369
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• 2008

본 연구의 목적은 다공성의 티타늄 산화막에 불소를 처리한 결과를 평가하는 것이다. 디스크에 양극산화법을 통하여 다공성의 티타늄산화막표면을 얻은 후 불소를 처리하고 일정시간동안 Hank액에 침전을 시켰다. 양극산화를 통해 일정거칠기의 표면을 얻었으며 SEM과 XRD를 통하여 표면의 형상과 성분을 분석하였다. 생성된 표면은 빠른 표면 활성도를 보였으며, 적절한 거칠기와 좋은 골반응으로 골유착에 기여할 수 있을 것으로 여겨진다.

• 한국식품과학회지
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• 제38권2호
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• pp.279-283
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• 2006

홍화씨의 폴리페놀 성분들이 HMGR 활성, LDL-oxidation과 Hep3B 세포로부터의 Apo A1 분비능에 미치는 영향을 조사하였다. 홍화씨 성분은 matairesinol(lignan), acacetin(flavone) 및 N-feruloylserotonin과 matairesinol의 대사산물인 enterolactone을 사용하였다. 그 결과, HMCR 저해활성은 mevastatin이 53%로 가장 높은 활성을 나타내었고 다음은 홍화씨 serotronin 유도체인 N-feruloylserotonin이 45%의 저해활성을 보여 홍화씨 폴리페놀 중에서는 가장 높은 활성을 보였으며 genistein은 35%의 활성을 보인 반면 나머지는 활성이 거의 미미하였다. N-feruloylserotonin의 활성은 용량 의존적 반응을 나타내었다. LDL 산화 억제효과는 사용한 모든 홍화씨 폴리페놀성분에서 나타났는데, 특히 N-feruloylserotonin에서 가장 강한 활성을 보였고 역시 용량 의존적 반응을 보였다. Hep3B에서의 Apo A1 분비능은 mevastatin에서 가장 높았고 enterolactone, genistein 및 ${\beta}-estradiol$에서 분비 증강활성을 나타내었다. 이상의 결과들로 미루어 볼 때, 홍화씨 폴리페놀 성분들은 콜레스테를 생합성과 LDL의 산화를 억제시킴으로서 지질대사 및 동맥경화 개선에 기여할 수 있을 것으로 사료된다.

• 한국식품과학회지
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• 제35권4호
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• pp.752-755
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• 2003

E. coli O157:H7에 대한 마늘즙액의 항균작용을 알아보기 위하여 마늘즙액의 처리량을 농도별 조건을 달리한 후 생균수 측정을 실시하였다. 마늘즙액 농도가 1%에서는 첨가하지 않았을 때에 비해 E. coli O157:H7의 생균수가 약간 줄어들었으나 마늘즙 처리량이 3%에서는 약 5 log, 마늘즙 처리량이 5%로 증가했을 때는 약 6 log의 생균수 감소를 보였다. 마늘즙의 식육내 항균작용 효과를 알아본 결과, 저육에서 3%, 6%, 10%의 마늘즙 농도별 차이에 따른 E. coli O157:H7에 대한 항균효과는 크게 다르지 않음을 알 수 있었다. 마늘즙의 가장 뚜렷한 저해 효과는 저장 9일에 나타나 약 2 log의 생균수 감소가 관찰되었다. 저장 9일 이후에는 E. coli O157:H7의 생균수가 다시 증가하는 것으로 미루어 마늘즙의 항균 효과가 소실되는 것으로 추정된다. 본 실험의 결과, 마늘의 조미료로써의 기능과 더불어 천연 방부제로써의 항균효과에 관한 기초 자료를 얻을 수 있었다.

• 대한약리학회지
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• 제20권2호
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• pp.49-57
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• 1984

The exocrine pancreatic secretion is controlled mainly by gastrointestinal hormones as well as cholinergic nerves. The adrenergic influence on exocrine pancreas is thought not to he important and the evidences supporting this contention are still contradictory. In an effort to elucidate the adrenergic influence on the exocrine pancreas, we have determined the amylase release from pancreatic slices of rats treated with adrenergic drugs. The albino rats of either sex, weighing $60{\sim}80\;g$, were decapitated and the uncinate pancreata were isolated and incubated in screw top vials containing 2 ml krebs-Ringer bicarbonate buffer solution gassed with 95% $O_2$ and 5% $CO_2$. These vials were shaken continuously in a waterbath maintained at $37^{circ}C$, and enzyme release was stimulated with acetylcholine$(10^{-5}M)$. For chronic treatment methoxamine$(an\;{\alpha}-adrenergic\;agonist,\;5\;mg/kg)$, isoproterenol (a\;{\beta}-adrenergic\;agonist,\;10\;mg/kg) and reserpine (0.5 mg/kg) along with cholecystokinin octapeptide$(CCK-op,\;2{\mu}g/kg)$ were given i.p. in rats daily for 3, 5, 7, 9 or 12 days. For acute experiment these drugs were added directly to the incubation medium in a concentration of $10^{-5}M$ except CCK-OP $(10^{-9}M)$. The results are summarized as follows. 1) The addition of methoxamine, isoproterenol or reserpine to the incubation medium containing pancreatic slices augmented the release of amylase induced by acetylcholine and among them the effect of isoproterenol was most prominent. 2) Chronic treatment of methoxamine or reserpine caused enhancement of acetylcholine response in amylase release from pancreatic slice throughout the experimental period, but the amylase release was less than that of control by 12 days isoproterenol treatment. 3) In the pancreatic slices obtained from 12 days treatment of CCK-OP, the amylae release responding to acetylcholine was enhanced. By these finding it is suggested that methoxamine, isoproterenol and reserpine had marked influence on the exocrine pancreatic functions in rats and that these effects are due to their inherent actions rather than sympathetic nerve or adrenergic receptor function.

• Animal Bioscience
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• 제34권6호
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• pp.975-984
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• 2021

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

• Journal of Veterinary Science
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• 제22권3호
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• pp.39.18-39.18
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• 2021

Background: Interferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear. Objectives: The purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV). Methods: The effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods. Results: In this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV. Conclusion: Expression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV.

• Asian-Australasian Journal of Animal Sciences
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• 제32권12호
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• pp.1942-1949
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• 2019

Objective: Leukocyte cell-derived chemotaxin 2 (LECT2) is associated with several physiological processes including inflammation, tumorigenesis, and natural killer T cell generation. Chicken LECT2 (chLECT2) gene was originally identified as one of the differentially expressed genes in chicken kidney tissue, where the chickens were fed with different calcium doses. In this study, the molecular characteristics and gene expression of chLECT2 were analyzed under the stimulation of toll-like receptor 3 (TLR3) ligand to understand the involvement of chLECT2 expression in chicken metabolic disorders. Methods: Amino acid sequence of LECT2 proteins from various species including fowl, fish, and mammal were retrieved from the Ensembl database and subjected to Insilco analyses. In addition, the time- and dose-dependent expression of chLECT2 was examined in DF-1 cells which were stimulated with polyinosinic:polycytidylic acid (poly [I:C]), a TLR3 ligand. Further, to explore the transcription factors required for the transcription of chLECT2, DF-1 cells were treated with poly (I:C) in the presence or absence of the nuclear factor ${\kappa}B$ ($NF{\kappa}B$) and activated protein 1 (AP-1) inhibitors. Results: The amino acid sequence prediction of chLECT2 protein revealed that along with duck LECT2 (duLECT2), it has unique signal peptide different from other vertebrate orthologs, and only chLECT2 and duLECT2 have an additional 157 and 161 amino acids on their carboxyl terminus, respectively. Phylogenetic analysis suggested that chLECT2 is evolved from a common ancestor along with the actinopterygii hence, more closely related than to the mammals. Our quantitative polymerase chain reaction results showed that, the expression of chLECT2 was up-regulated significantly in DF-1 cells under the stimulation of poly (I:C) (p<0.05). However, in the presence of $NF{\kappa}B$ or AP-1 inhibitors, the expression of chLECT2 is suppressed suggesting that both $NF{\kappa}B$ and AP-1 transcription factors are required for the induction of chLECT2 expression. Conclusion: The present results suggest that chLECT2 gene might be a target gene of TLR3 signaling. For the future, the expression pattern or molecular mechanism of chLECT2 under stimulation of other innate immune receptors shall be studied. The protein function of chLECT2 will be more clearly understood if further investigation about the mechanism of LECT2 in TLR pathways is conducted.

• 대한본초학회지
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• 제33권5호
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• pp.105-110
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• 2018

Objectives : We tried to observe the fluorescence anisotropy and intensity of ethidium ion in the intercalating binding interaction between DNA and ethidium ions in the presence of berberine, and then tried to explain the effect of berberine on the intercalating interaction of ethidium ion with DNA. Methods : DNA(calf thymus DNA), berberine and ethidium bromide(EtBr) were purchased from Sigma-Aldrich Co. Proper amount of each compound was dissolved in 20 mM sodium phosphate buffer(pH 7.0) containing 100 mM of NaCl to prepare stock solutions. Collections of the fluorescence anisotropy and intensity data were performed on JASCO FP-8300 spectrofluorometer equipped with a polarizer and a Peltier temperature controller. The excitation of ethidium ion was done at 550 nm and the emission data were collected at 600 nm. For Stern-Volmer plot, the fluorescence data were collected at $18^{\circ}C$ and $30^{\circ}C$. Results : According to the results of this research, the weak competitive binding pattern between ethidium ion and berberine appeared in binding with DNA at low ratio of DNA to ethidium ion. But at high ratio of DNA to ethidium ion, this weak competition disappeared. Instead, berberine might bind to DNA by intercalating way. In other words, berberine could de-intercalate ethidium ion from DNA at low concentration of DNA relative to ethidium ion, but could not at high concentration of DNA relative to ethidium ion. In addition, the mechanism of fluorescence quenching of ethidium ion could also proceed differently, depending on the ratio of the amount of DNA to that of ethidium ion. Conclusions : The effect of berberine on the DNA-ethidium ion intercalating interaction could work differently, depending on the relative ratio of the amount of DNA to that of ethidium ion. This study also showed that fluorescence anisotropy analysis is very useful method to obtain detailed information for investigation of the complex binding interactions. In order to fully understand the mechanism of action of the pharmacological effect by berberine, studies on the effect of berberine on the action of proteins such as various enzymes closely related to berberine-induced medicinal effects should be continued.