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The Effect of Polyphenols from Safflower Seed on HMG-CoA Reductase (HMGR) Activity, LDL Oxidation and Apo A1 Secretion  

Cho, Sung-Hee (Department of Food Science and Nutrition, Catholic University of Daegu)
Park, Young-Yi (Department of Food Science and Nutrition, Catholic University of Daegu)
Yoon, Ji-Young (Department of Food Science and Nutrition, Catholic University of Daegu)
Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu)
Ha, Tae-Youl (Food Function Research Division, Korea Food Research Institute)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.2, 2006 , pp. 279-283 More about this Journal
Abstract
This study was conducted to examine the effect of polyphenols from safflower seed on HMG-CoA reductase (HMGR) activity, LDL oxidation and Apo A1 secretion from Hep3B cell. The safflower seed polyphenols were matairesinol (Iignan), enterolactone (lignan metabolite), acacetin (flavone) and serotonin derivative. In addition to safflower polyphenols, mevastatin, ${\alpha}-estradiol,\;{\alpha}-tocopherol$ and soy genistein were tested as reference compounds depending on the type of the test. HMGR source was liver microsome obtained from rat fed 2% cholestyramine for 10 days. Inhibition of HMGR activity was greater with mevastatin (53%) than safflower serotonin derivatives (45%), followed by genistein (35%), but was very small with matairesinol, enterolactone and acacetin. LDL oxidation induced by $CuSO_4$ was suppressed by all the test material used in the present study and in the order of safflower serotonin derivatives> matairesinol > ${\beta}-estradiol$ > genistein > acacetin > enterolactone. Apo A1 secretion from Hep3B cell was significantly stimulated by mevastatin, but moderately (p<0.1) by ${\beta}-estradiol$ and genistein as well as enterolactone. These results suggest that the safflower polyphenols improve body lipid status via inhibition of cholesterol synthesis and suppression of LDL oxidation.
Keywords
safflower polyphenol; HMG-CoA reductase; Apo A1; LDL oxidation;
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1 Ha TY, Cho IJ, Lee SH. Screening of HMG-CoA reductase inhibitory activity of ethanol and methanol extracts from cereals and legumes. Korean J. Food Sci. Technol 30: 224-229 (1998)   과학기술학회마을
2 Moon KD, Back SS, Kim JH, Jeon SM, Lee MK, Choi MS. Safflower seed extract lowers plasma and hepatic lipids in rats fed high-cholesterol diet. Nutr. Res. 21: 895-904 (2001)   DOI   ScienceOn
3 Segreat J, Li L, Ananthatamaiah GM. Harvey SC, Liadaki KN, Zannis V. Structure and function of apolipoprotein A-l and high-density lipoprotein. Curr. Opin. Lipidol. 11: 105-115 (2000)   DOI   ScienceOn
4 Bonn V, Cheung RC, Chen B. Taghibiglou C. van Iderstine SC, Adeli K. Simvastatin, an HMG-CoA reductase inhibitor, induces the synthesis and secretion of apolipoprotein Al in HepG2 cells and primary hamster hepatocytes. Atherosclerosis 163: 59-68 (2002)   DOI   ScienceOn
5 Tam SP, Archer YK, Deeley RG. Effects of estrogen on apolipoprotein secretion by the human hepatocarcinoma cell line, HepG2. J. Biol. Chem. 260: 1670-1675 (1985)
6 Lamon-Fava S. Genestein activates apolipoprotein A-I gene expression in human hepatoma cell line HepG2. J. Nutr. 130: 2489-2492 (2000)   DOI
7 Witzum JL. The role of monocytes and oxidized LDL in atherosclerosis. vol 21, pp. 59-69. In: Atherosclerosis Reviews. Leaf A, Weber PC (eds). Raven Press, New York, USA (1990)
8 Rotheneder M, Puhl H, Waeg G. Striegl G. Esterbauer H. Effect of oral supplementation with D-a-tocopherol on the vitamin E content of human low density lipoprotein and resistance to oxidation. J. Lipid Res. 32: 1325-1332 (1991)
9 Shapiro DJ, Nordstrom JL, Mitschele JJ, Rodwell VW. Schimke RT. Micro assay for 3-hydroxy-3-methylglutaryl CoA reductase in rat liver and in L-cell fibroblasts. Biochim. Biophys. Acta. 370: 369-377 (1974)   DOI
10 Hirano R, Kondo K, Iwamoto T, Igarashi O, Itakura H. Effects of antioxidants on the oxidative susceptibility of low-density lipoprotein. J. Nutr. Sci. Vitaminol. 43: 435-444 (1997)   DOI   ScienceOn
11 Balasubashini M, Rukkumani R, Viswanathan P, Menon VP. Ferulic acid alleviates lipid peroxidation in diabetic rats. Phytother. Res. 18: 310-314 (2000)   DOI   ScienceOn
12 Klensek DA, Dugan RE, Baker TA, Porter JW. 3-Hydroxy-3-methylglutaryl coenzyme A reductase from rat liver. Methods Enzymol. 71: 462-79 (1981)   DOI
13 Kamal-Eldin A, Fran J, Razdan A, Tengbad S, Basu S, Vessby B. Effects of dietary phenolic compounds on tocopherol, cholesterol, and fatty acids in rat. Lipids 35: 427-435 (2000)   DOI   ScienceOn
14 Horner NK, Kristal AR, Prunty J, Skor HE, Potter JD, Lampe JW. Dietary determinant of plasma enterolactone. Cancer Epidemiol. Biomarkers Prev. 11: 121-126 (2002)
15 Cho SH, Lee HR, Choi SW, Lee WJ, Choi Y. Formulated product containing safflower seed extract improves lipid status of ovariectomized rats. Korean J. Gerentol. 15: 39-46 (2005)
16 Setchell KD. Adlercreutz H. Mammalian lignans and phytoestrogens. Recent studies on their formation, metabolism, and biological role in health and disease. pp, 315-345. In: Role of the Gut Flora in Toxicity and Cancer. Rowland I (ed). Academic Press, London, England (1988)
17 Kim HK, Jeong TS, Lee MK, Park YB, Choi MS. Lipid-lowering efficacy of hesperetin metabolites in high-cholesterol fed rats. Clinica. Chimica. Acta. 327: 129-137 (2003)   DOI   ScienceOn
18 Kilkkinen A. Valsta LM. Virtamo J, Stumpf K, Adlercreutz H, Pietinen P. Intake of lignans is associated with werum enterolactone concentration in Finnish men and women. J. Nutr. 133: 1830-1833 (2003)
19 Kim HJ, Bae YC, Park RW, Choi SW, Cho SH, Choi YS, Lee WJ. Bone-protecting effect of safflower seeds in ovariectomized rats. Calcif. Tissue Int. 71: 88-94 (2002)   DOI   ScienceOn
20 von Echardstein A, Assemann G. Prevention of coronary heart disease by rasing high-density lipoprotein cholesterol. Curr. Opin. Lipidol. 11: 627-637 (2000)   DOI   ScienceOn
21 Kang GH, Chang EJ, Choi SW. Antioxidative activity of phenolic compounds in roasted safflower (Carthamus tinctorious L.) seeds. J. Food Sci. Nutr. 4: 221-225 (1999)   과학기술학회마을
22 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275 (1951)
23 Ndong-Akoume MY, Mignault D, Perwaiz S, Plaa GL, Yousef IM. Simultaneous evaluation of HMG-CoA reductase and cholesterol $7{\alpha}$-hydroxylase activites oy electrospray tandem MS. Lipids 37: 1101-1107 (2002)   DOI   ScienceOn
24 Hendriks HF, Veenstra J, van Tol A, Greener JF. Schaafsma G. Moderate doses of alcoholic beverage with diner and post-prandial high density lipoprotein composition. Alcohol 33: 403-410 (1998)
25 Jin FY, Kamanna VS, Kashyap ML. Estradiol stimulates apolipoprotein A-I but not A-II containing particle synthesis and secretion by stimulating mRNA trascription rate in HepG2 cells. Arterioscle. Thromb. Vasc. Biol. 18: 999-1006 (1998)   DOI   ScienceOn
26 Cho SH, Jung SE, Lee HK. Ha TY.. Effects of methanol extract of prosomillet on cholesterol and fatty acid metabolism in rat. J. Food Sci. Nutr. 3: 188-192 (1999)
27 Cho SH, Lee HR. Kim TH. Choi SW, Lee WJ, Choi Y. Effects of defatted safflower seed extract and phenolic compounds in diet on plasma and liver lipid in ovariectomized rats fed high cholesterol diets. J. Nutr. Sci. Vitaminol. 50: 32-37 (2004)   DOI   ScienceOn
28 Meng Q, Lewis P, Wahala K, Adlercreutz H, Tikkanen. Incorporation of esterified soybean isoflavone with antioxidant activity into low density lipoprotein. Biochim. Biophys. Acta. 1438: 369-376 (1999)   DOI   ScienceOn
29 Fungwe TV, Kudchodkar BJ, Lacko AG, Dory L. Fatty acids modulate lecithin:cholesterol acyltransferase secretion independent of effects on triglyceride secretion in primary rat hepatocytes. J. Nutr. 128: 1270-1275 (1998)
30 Lamon-Fava S. High-density lipoprotein: Effects of alcohol, estrogen and phytoestrogens. Nutr. Rev. 60: 1-7 (2002)   DOI   ScienceOn
31 Cho SH, Choi SW, Choi Y, Lee WJ. Effects of defatted safflower and perilla seed powders on lipid metabolism in ovariectomized female rats fed high cholesterol diets. J. Korean Soc. Food Sci. Nutr. 30: 112-118 (2001)   과학기술학회마을
32 Chen CY, Milbury PE, Kwak HK, Collins FW, Samuel P, Blumber JB. Avenanthramides and phenolic acids from oats are bioavailable and act synergistically with vitamin C to enhance hamster and human LDL resistance to oxidation. J. Nutr. 134: 1459-1466 (2004)