• 대한약침학회지
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• 제5권1호
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• pp.61-79
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• 2002

Objective: The purpose of this study was to investigate acute and subacute toxicity and sarcoma-180 anti-cancer effects of Herbal acupuncture with Anneniacae amarum semen (Haeng-in) in mice and rats. Method: Balble mice were injected intraperitoneany with Haeng-In extract for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were injected intraperitoneally with Haeng-In extract for subacute toxicity test. TheAnneniacae amarum semen Herbal-Acupuncture was injected on Chung-wan (CV12) of mice with Sarcoma-180 cancer cell line. Results: 1. $LD_{50}$ was uncountable as none of the subjects expired from the treatment groups during the test. 2. The clinical signs and the body weight of mice treated with 0.1cc and 0.2cc Haeng-In extract were not affected during the acute toxicity test. 3. In acute toxicity test of serum biochemical values of mice, total protein and albumin were decreased in treatment group Ⅰ. Glucose was increased, and total cholesterol was decreased in treatment groups. GPT was increased in treatment group Ⅰ. 4. In subacute toxicity test, toxic symptoms were not detected in the treatment groups. 5. In subacute toxicity test, the body weight was increased in treatment groups on 14th and 21st day. 6. In subacute toxicity test. liver weight was increased in treatment group Ⅱ, and spleen weight was increased in treatment group Ⅱ. Lung weight was increased in an the treatment groups.(p<0.05) 7. In subacute toxicity test, severe tissue injury was found in lung and liver, especially treatment group Ⅰshowed more significant lung damage compared to treatment group l. 8. In subacute toxicity test, WBC. MCH and MCHC were increased in an the treatment groups, RBC, HGB and HCT were decreased in treatment group H(p<0.05). 9. In subacute toxicity test of serum biochemical values of rats, triglyceride was decreased in all the treatment groups. ALP was decreased in treatment group Ⅰ. and creatinine was decreased in treatment group Ⅱ. BUN/CR was increased in treatment group Ⅱ(p<0.05). 10. Median survival time of Sarcoma-180 cancer cell treated with Haeng-In was increased in all the treatment groups by twenty percent, compared to the control group(p<0.05). 11. Natural killer cell activity about the Sarcoma-180 cell was decreased at the ratio of 100:1 but was increased at the ratio of 10:1. In treatment group Ⅱ, increase was found at the ratio of 100:1 and 50:1 (p<0.05). 12. Interleukin-2 productivity of the Sarcoma-180 cell was decreased in treatment group I, but was increased in treatment group Ⅱ(p<0.05). Conclusion: According to the results, we can conclude Herbal-acupuncture with Anneniacae amarum semen caused toxicity, and had effects in Sarcoma-180 cancer cell.

• 한국식품위생안전성학회지
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• 제13권2호
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• pp.77-82
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• 1998

생약제 농축액의 안정성 확보하기 위한 기초자료를 제공하고자 생약제 농축액에서 수분 활성도 변화에 따른 미생물 성장 변화 및 일부 이화학적 특성 변화를 조사하였다. 수분 활성도를 각각 0.86, 0.89, 0.69로 조정한 생약제 농축액을 $40^{\circ}C$에서 180일 동안 저장한 후 세균수의 생균수를 측정한 결과 수분활성도 0.86(수분 활성도 63.85%) 시료의 경우 초기 생균수가 18/g에서 90일 저장시 80/g으로 180일 저장시 190/g으로 증가하였다. 수분활성도 0.80(수분함량 43.04%) 경우에는 초기생균수가 24/g에서 90일 저장시 83/g으로, 180일 저장시 170/g으로 증가하였다. 그러나 수분활성도 0.69(수분함량 33.05%) 경우에는 초기 생균수가 16/g에서 90일 및 180일 저장시 각각 2-/g, 25/g으로 큰 균수가 증가를 나타내지 않았다. 한편 수분 활성도에 따른 병원성 미생물의 성장은 수분활성도가 낮을수록 저해되었으며 C. albicans, A. niger의 경우 $28^{\circ}C$에서 30일 저장 후 초기 생균수가 150/g, 140/g에서 각각 30/g, 20/g으로 크게 감소하였고, E. coli, S. aureus, P. aeruginosa의 경우에도 수분활성도 0.69 시료에서는 $37^{\circ}C$에서 30일 저장 후 생균수가 0로 나타나서 균은 완전히 사멸되었다. 수분활성도 0.86, 0.80, 0.69 시료에서 pH의 변화는 180일 저장시의 pH에서 초기 pH를 뺀 pH의 감소 폭이 각각 0.84, 0.72, 0.31로 나타나서 수분활성도가 낮을수록 pH의 감소폭이 적은 경향이었다. 생약제 농축액에 포함된 인삼 중의 지표성분인 사포닌은 180일 저장한 시료에서 측정한 사포닌함량에서 저장 초기 시료의 함량을 뺀 사포닌 함량의 감소폭은 0.12~0.30%로 나타나서 비교적 작은 편이었다. 또한 생약제 농축액에 감미료, 산미료 등의 첨가물을 첨가하여 $40^{\circ}C$에서 60일 및 180일 동안 저장한 후 TLC로 사포닌을 조사한 결과 모든 사포닌이 확인되어 이러한 조건에서 사포닌은 안정한 것으로 생각된다.

• 한국축산식품학회지
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• 제40권1호
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• pp.87-95
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• 2020

In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37℃ until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC₅₀) was 2,817 ㎍/mL.

• 한국융합학회논문지
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• 제13권5호
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• pp.287-294
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• 2022

본 연구는 스포츠 현장에서 부상예방으로 많이 사용되고 있는 탄력 테이핑을 슬관절 굴곡과 신전에 적용하여 근력, 근파워, 근지구력에 미치는 영향을 검증하기 위해 진행하였다. 연구 대상자는 슬관절의 굴곡과 신전 운동에 이상이 없는 남자 대학생 10명으로 하였다. 측정방법은 등속성근기능측정 장비를 활용하여 슬관절 굴근과 신근에 키네시오 테이핑을 적용 전, 후로 각속도 60°/sec 5회, 180°/sec 5회, 240°/sec 25회를 측정하였다. 연구결과는 슬관절 신전 시 키네시오 테이핑 적용 전보다 후에 우측 각속도 180°/sec에서 Average Power가 유의하게 증가하였다(p=.010). 슬관절 굴곡 시 키네시오 테이핑 적용 전보다 후에 우측 각속도 180°/sec에서 Average Power가 유의하게 증가하였다(p=.016). 또한 각속도 240°/sec에서는 Total Work Done에서 좌(p=.002), 우(p=.002) 모두 유의하게 증가하였다. 대퇴직근과 대퇴이두근에 키네시오 테이핑 적용이 슬관절 운동 시 근파워과 근지구력을 향상시키는 보조적인 영향이 긍정적으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.789-795
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• 2004

A promoter-probe shuttle vector pSK1Cat was constructed for the isolation of transcriptional signal sequences from Corynebacterium glutamicum. Besides conferring resistance to kanamycin in Escherichia coli and C. glutamicum, the vector carried a promoterless cat gene to confer resistance to chloramphenicol upon insertion of the appropriate transcriptional signals in the multiple cloning site. By utilizing the vector, a series of transcriptionally active fragments were isolated from the genome of C. glutamicum. The clones, ranging from 200 bp to 1 kb in size, were grouped into 3 classes of strong, medium, and weak, based on the chloramphenicol acetyltransferase (CAT) activity and sensitivity to the chloramphenicol of the clone-carrying C. glutamicum cells. C. glutamicum cells carrying the $P_{19}$ clone, a representative in the strong class, were able to grow on minimal agar plates containing over $40 mg/mell$ chloramphenicol, and showed CAT activity of 10 m㏖/mgㆍmin, performing slightly better than the cells carrying $P_{tac}$ , a strong E. coli promoter. Subcloning analysis of the $P_{19}$ clone identified a 180 bp intergenic fragment ($P_{180}$), which was located upstream of a gene encoding a hypothetical membrane protein. The expression conferred by $P_{180}$ was not affected by either the kinds of carbon sources or changes in temperature. These properties make the $P_{180}$ clone useful for the deregulated expression of biosynthetic genes in C. glutamicum during amino acid fermentation.

• 약학회지
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• 제45권6호
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• pp.641-649
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• 2001

In this study, the antitumor activities were investigated using $\beta$-lmmunan, a proteoglycan obtained from cultured mycelia of the IY009 strain of Ganoderma lucidum belonging to basidiomycetes. The result showed the significant effect of cytotoxicity test against murine sarcoma 180 and murine lymphocytic leukemia L1210 using immunized macrophage cultures by $\beta$-lmmunan. When intraperitoneally injected at 40 mg/kg/day daily for 10 days, $\beta$-lmmunan inhibited the growth of sarcoma 180 solid tumor in ICR mice by 88.8% (p<0.05). It was also observed that $\beta$-lmmunan increased life span by 85.2% (p<0.01) after treatment of 100 mg/kg/day in BDF1 mice bearing lymphocytic leukemia L1210. And combination therapy with cisplatin (dosage: 4 mg/kg) increased life span by 140.4% (p<0.05) after treatment of 100 mg/kg/day daily in BDF1 mice bearing lymphocytic leukemia L1210.

• 한국염색가공학회지
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• 제10권3호
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• pp.50-56
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• 1998

Nonwoven fabric was treated with chitosan solution to impart antimicrobial activities by pad-dry method. Antimicrobial activity was measured by Shake Flask Method. Two chitosans of different molecular weight(Mw) with similar degree of deacetylation(DDA) were used : ca. 1,800(chitosan oligomer : DDA 84%) and 180,000(DDA 86%). Chitosan oligomer displayed high antimicrobial activity against P. vulgaris at 0.01%, S. aureus and E. coli at 0.05% treatment concentration, shelving above 90% of reduction rate. Chitosan of Mw 180,000 was effective against S. aureus, E. coil and P. vulgaris at 0.05% treatment concentration, showing almost 100% reduction rate. While chitosan of Mw 180,000 shelved reduction rate above 75% against K. pneumoniae and P. aeruginosa at 0.5% treatment concentration, chitosan oligomer was not effective against them. Fabrics become stiffer and less air permeable as treatment concentration increases. Liquid strike-through time of the sample treated with 0.5% chitosan oligomer solution (3.0 sec) was comparable with a hydrophilic finished sample commercially available(2.6 sec).

• 미생물학회지
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• 제25권3호
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• pp.173-179
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• 1987

한국산 A, tumefaciens의 3균주에서 분리한 Ti plasmid의 type과 분자량을 조사한 결과로 이들 모두는 octopine type 이었으며 각 plasmid의 분자량은 pTi12가 44Kb이었고, pTi14는 180Kb이었으며, pTi14는 172Kb이었다. 이중 pTi12를 Smal 및 HindIII로 가수분해한 결과 8개의 Smal digest fragment와 10개의 Hind III digest fragment를 얻었으며 Southern hybridization techniques을 이용하여 잠정적인 physical map을 작성하였다.

• 공업화학
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• 제26권2호
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• pp.159-164
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• 2015

Benzimidazolone계 황색 안료 Benzimidazolone 180 (P.Y. 180)은 잉크, 도료, 플라스틱, 토너, 칼라필터 등의 다양한 분야에서 널리 사용되며, 녹색 빛을 나타내는 황색 안료로 내열성, 내용제성 및 내산 염기성에 우수한 고기능성 안료이다. 본 연구에서는 커플링 반응을 통하여 다양한 온도 조건 하에서 합성하였으며 가압 장치 autoclave를 사용하여 여러 용매 및 온도별 결정화 처리를 통하여 시료의 물성에 미치는 영향을 고찰하였다. DMSO 용매를 사용하여 결정화 처리한 안료는 상대적으로 높은 회절 강도 비와 입자크기 증가, 분산성 향상, 색력 증가를 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.681-685
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• 2009

L-carnitine promotes mitochondrial ${\beta}$-oxidation of long chain fatty acids and their subsequent transport across the inner mitochondrial membrane. Although the role of L-carnitine in fatty acid metabolism has been extensively studied, its role in live performance and carcass responses of commercial broilers is less understood. The objective of this research was to determine if Lcarnitine fed at various levels in diets differing in CP and amino acids impacted on live performance and carcass characteristics of commercial broilers. Two floor pen experiments were conducted to assess the effect of dietary L-carnitine in grower diets. In Exp. 1, Ross${\times}$Hubbard Ultra Yield broilers were placed in 48 floor pens (12 birds/pen) and fed common diets to d 14. A two (0 or 50 ppm Lcarnitine) by three (173, 187, and 202 g/kg CP) factorial arrangement of treatments was employed from 15 to 35 d of age (8 replications/treatment). An interaction (p<0.05) in carcass yield indicated that increasing CP (187 g/kg) resulted in improved yield in the presence of L-carnitine. Increasing CP from 173 to 202 g/kg increased (p<0.05) BW gain and decreased (p<0.05) feed conversion and percentage abdominal fat. Feeding dietary L-carnitine increased back-half carcass yield which was attributable to an increase (p<0.05) in thigh, but not drumstick, yield relative to carcass. In Exp. 2, $Ross{\times}Ross$ 708 broilers were fed common diets until 29 d. From 30 to 42 d of age, birds were fed one of seven diets: i) 200 g/kg CP, 0 ppm L-carnitine; ii) 200 g/kg CP, 40 ppm L-carnitine; iii) 180 g/kg CP, 0 ppm L-carnitine; iv) 180 g/kg CP, 10 ppm L-carnitine; v) 180 g/kg CP, 20 ppm L-carnitine; vi) 180 g/kg CP, 30 ppm L-carnitine; and vii) 180 g/kg CP, 40 ppm L-carnitine (6 replications of 12 birds each). BW gain, feed conversion, mortality (30 to 42 d), and carcass traits (42 d) were measured on all birds by pen. There were no treatment differences (p<0.05). However, the addition of 40 ppm L-carnitine in the 200 g CP/kg diet increased (p = 0.06) thigh yields relative to BW in comparison to birds fed diets without L-carnitine, which was further confirmed via a contrast analysis (0 vs. 40 ppm L-carnitine in the 200 and 180 g CP/kg diets; p<0.05). These results indicated that dietary L-carnitine may heighten metabolism in dark meat of commercial broilers resulting in increased relative thigh tissue accretion without compromising breast accretion.