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Postoperative Echocardiographic Hemodynamic Comparison between Recently Available Bileaflet Mechanical Valves (수종의 기계판막치환후 초음파심음향도를 이용한 판막간의 혈류역학적 비교)

  • Kang Joon Kyu;Hong Joon Hwa;Kim Hyung Tai;Park In Duk;Lee Cheol Joo
    • Journal of Chest Surgery
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    • v.38 no.7 s.252
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    • pp.496-500
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    • 2005
  • There was no difference between the bileaflet mechanical valves on the midterm and longterm clinical outcome. We reviewed the hemodynamic comparison between recently available mechanical valves by Doppler Echocardiography. Material and Method: We retrospectively reviewed 396 postoperative hemodynamic datas (EOA, MDPG, and MSPG) by doppler echocardiography in 345 patients. Mechanical valves from 5 venders (Sorin Bicarbon, SJM, ATS, On-X, and Edward MIRA) were compared. There were 232 valves in mitral position, 162 in aortic, and 2 in tricuspid. Result: There were 178 men (mean age; $50.6\pm13.9$ years old) and 167 women $(52.6\pm,4.6)$. MDPG/EOA of 27 mm in mitral position was Sorin; $4.2\pm1.5 mmHg/3.0\pm0.9cm^2,\;SJM;\;2.3\pm1.2/3.5\pm0.6$. In 29mm, Sorin, SJM, ATS, On-X, MIRA revealed $3.4\pm1.2/3.1\pm0.6,\;3.3\pm1.1/2.7\pm0.4,\;3.8\pm0.8/3.2\pm0.6,\;4.0\pm3.0/3.1\pm0.9,\;2.9\pm0.9/3.0\pm0.8$ In 31mm, Sorin, SJM, ATS, MIRA revealed $3.9\pm1.9/2.9\pm0.6,\;3.5\pm1.2/3.0\pm0.6,\;3.4\pm0.8/2.8\pm0.2,\;3.7\pm1.5/2.7\pm0.7$. In 33mm, Sorin, SJM, MIRA revealed $4.4\pm0.9/2.5\pm0.4,\;3.4\pm1.5/3.3\pm0.5,\;4.7\pm2.4\3.0\pm0.3$. MSPG/EOA of 19mm aortic position was Sorin, SJM, ATS, On-X, MIRA $18.0 mmHg/1.2cm^2,\;25.6\pm8.7/1.1\pm0.3,\;25.9\pm12.6/1.2\pm0.3,\;23.0/1.3,\;27.9\pm7.1/1.2\pm0.1$ in that order. In 21mm, SJM, ATS, On-X, MIRA revealed $18.3\pm6.7/1.5\pm0.5,\;13.7\pm2.1/1.7\pm0.3,\;17.0/1.4,\;17.1\pm5.5/1.8\pm0.5$. In 23mm Sorin, SJM, ATS, On-X, MIRA revealed $14.0\pm4.6/1.7\pm0.6,\;12.8\pm3.2/2.0\pm0.2,\;16.8\pm12.2/2.1\pm0.9,\;14.0/1.5,\;15.0\pm5.5/1,8\pm0.5$. In 25mm, SJM and MIRA revealed $14.0\pm5.1/1.8\pm1.0,\;11.0/2.3$. There was no statistically significant difference in these values between the venders given the same position and size. 2 redo valve replacements were performed, 1 due to severe hemolysis in ATS and 1 due to leaflet immobilization in SJM. Conclusion: Postoperative hemodynamic comparison by doppler echocardiography shows no statistically significant difference between recently available mechanical valves in this country.

Physicochemical Characteristics of Traditional Fermented Soybean Products Manufactured in Folk Villages of Sunchang Region (순창 고추장민속마을에서 생산한 전통 장류의 이화학적 특성)

  • Kim, Jong-Wook;Kim, Yong-Suk;Jeong, Pyeong-Hwa;Kim, Hyung-Eun;Shin, Dong-Hwa
    • Journal of Food Hygiene and Safety
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    • v.21 no.4
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    • pp.223-230
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    • 2006
  • For standardization of quality of traditional fermented soybean products manufactured in Folk Villages of Sunchang Region, the physicochemical characteristics of 28 Kochujang, 28 Doenjang, and 18 Chunggukjang were compared. Moisture contents of Kochujang, Doenjang, and Chunggukjang were $46.9{\pm}3.6,\;60.6{\pm}1.9,\;and\;57.0{\pm}3.10%$, respectively. On the basis of average moisture contents, crude protein, crude fat, and crude ash contents were calculated to $6.2{\pm}0.7,\;2.0{\pm}0.5,\;and\;8.2{\pm}1.1%$ in Kochujang, $13.2{\pm}1.0,\;7.1{\pm}0.6,\;and\;15.2{\pm}1.5%$ in Doenjang, and $18.9{\pm}1.2,\;6.1{\pm}1.4,\;and\;5.1{\pm}1.7%$ in Chunggukjang, respectively. Reducing sugar, salinity, and water activities in Kochujang were $19.25{\pm}4.1%,\;7.3{\pm}1.1%,\;and\;0.790{\pm}0.003$, in Doenjang were $2.38{\pm}0.89%,\;14.2{\pm}1.4%,\;and\;0.835{\pm}0.020$, and in Chunggukjang were $0.51{\pm}0.24%,\;4.2{\pm}1.6%,\;and\;0.962{\pm}0.028$, respectively. Amino-type nitrogen contents, which affects delicate flavors of fermented soybean products, of Kochujang, Doenjang, and Chunggukjang were $114.03{\pm}19.04,\;734.32{\pm}147.70,\;and\;600{\pm}150mg%$, respectively. Lightness (l), redness (a), and yellowness (b) values in color of Kochujang were $14.49{\pm}1.44,\;15.45{\pm}1.77,\;and\;8.34{\pm}1.02$, respectively, and the redness was lower than that of other ones. Those of Doenjang were $26.69{\pm}4.33,\;7.25{\pm}1.03,\;and\;12.02{\pm}1.82$, respectively, and those of Chunggukjang were $35.62{\pm}2.05,\;6.31{\pm}0.37,\;and\;13.50{\pm}0.78$, respectively. These results indicate that the salt concentration and quality of traditional fermented soybean products manufactured in Folk Villages of Sunchang region must be lowered and standardized, respectively.

Plasma Protein Profile of Neonatal Buffalo Calves in Relation to the Protein Profile of Colostrum/Milk during First Week Following Parturition

  • Lone, Abdul Gani;Singh, Charanbir;Singha, S.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.3
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    • pp.348-352
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    • 2003
  • An investigation was made into the protein profile of colostrum/milk of ten Murrah buffaloes and of their ten buffalo calves during their first week of neonatal life to study the materno-neonatal transfer of immunoglobulins (Ig). Calves were pail fed 3.5 liter of colostrum and/or milk per calf/day exclusively from their dam. First blood sample from newborn calves was collected before colostrum feeding on the day of birth (day zero) and the sampling continued daily for seven days after colostrum/milk feeding. Colostrum/milk Ig and IgG values were $4.82{\pm}2.60$, $2.19{\pm}1.90$, $1.12{\pm}0.82$, $0.69{\pm}0.44$, $0.59{\pm}0.31$, $0.47{\pm}0.20$, $0.40{\pm}0.22$, $0.40{\pm}0.25$ and $3.58{\pm}1.90$, $1.08{\pm}0.92$, $0.52{\pm}0.40$, $0.31{\pm}0.20$, $0.27{\pm}0.14$, $0.22{\pm}0.08$, $0.18{\pm}0.09$, $0.14{\pm}0.08$ respectively during 0-7 days post partum. The concentration of total colostrum/milk proteins, Ig, IgG and albumin were highest within 12 h post-partum. Thereafter, the concentrations followed a declining trend which may be attributed to the reduced transfer of proteins from the maternal blood, declining synthesis by the mammary glands and/or depletion of stored proteins. The concentrations of plasma Ig and IgG before colostrum feeding on day zero were $0.42{\pm}0.09$ and $0.08{\pm}0.03$ respectively. The levels of plasma Ig were $1.90{\pm}0.37$, $1.80{\pm}0.31$, $1.80{\pm}0.26$, $1.81{\pm}0.28$, $1.78{\pm}0.31$, $1.79{\pm}0.21$, $1.80{\pm}0.32$ and of IgG were $1.57{\pm}0.41$, $1.30{\pm}0.29$, $1.31{\pm}0.21$, $1.27{\pm}0.18$, $1.23{\pm}0.21$, $1.23{\pm}0.16$, $1.26{\pm}0.21$ on days 1-7 after birth after colostrum/milk feeding. The concentrations of total plasma proteins, Ig, IgG were lowest before colostrum feeding and increased significantly (p<0.05) after colostrum feeding in buffalo neonates. The results suggest that the highest amounts of colostral Ig and IgG were available on the day of parturition and thus the calves should receive colostrum as early after birth as possible. Colostrum Ig and IgG concentrations were not correlated to plasma Ig and IgG concentrations in the post-suckle buffalo calves and therefore, colostrum Ig and IgG concentrations were probably not the principle determinants of calf post-suckle plasma Ig and IgG concentrations.

Effect of Exercise and Diet Control Program on Nitrogen Balance in Obese Children (단기간의 운동 및 식이 조절이 비만아동의 질소 평형에 미치는 영향)

  • 박소앙;이성숙;최인선;오승호
    • Journal of Nutrition and Health
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    • v.34 no.6
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    • pp.637-644
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    • 2001
  • The purpose of this study was to examine the effect of exercise and diet control program on nitrogen balance in obese children. The subjects of this study were five obese boys aged from 8 to 12 participating in the control period(C) for 3 days, exercise without energy deficit period(EEN) for one week, and exercise with energy deficit period(EED) far another one week, consecutively. The intensity of the exercise was 60-70% of HRmax and the energy was restricted at 493 kcal/day. Daily mean total energy intake was 2,152 $\pm$ 138kca1, 1,861 $\pm$ 138kca1, and 1,368 $\pm$ 87kca1 for the period C, EEN and EED, respectively. The body weight for EEN and EED decreased compared with the period C by average 1.2 $\pm$ 0.2kg and 1.8 $\pm$ 0.3kg, respectively. Of the body weight loss, the fat mass decreased bur 1.0 $\pm$ 0.3kg and 1.5 $\pm$ 0.5kg and the lean body mass by 0.2 $\pm$ 0.4kg and 0.3 $\pm$ 0.4kg, respectively. Of the daily mean nitrogen consumption was 9.1 $\pm$ 0.7g, 9.1 $\pm$ 0.8g and 7.1 $\pm$ 0.5g, the daily mean nitrogen excretion from feces was 1.3 $\pm$ 0.2g, 0.9 $\pm$ 0.1g and 0.7 $\pm$ 0.1g, and from urine was 6.7 $\pm$ 0.6g, 6.6 $\pm$ 0.5g and 6.7 $\pm$ 0.6g, for the period C, EEN and EED, respectively. Daily mean nitrogen balance excluding nitrogen excretion firm feces and urine indicated + 1.1 $\pm$ 1.0, +1.6 $\pm$ 0.6 and - 0.3 $\pm$ 0.9 for the period C, EEN and EED, respectively. Despite the negative indication during EED, there was no statistical signifiranre. The daily mean creatinine excretion was 0.5 $\pm$ 0.0g, 0.7 $\pm$ 0.1g and 0.6 $\pm$ 0.0g and the creatinine-height index(CHl) was 85.1 $\pm$ 6.7%, 83.8 $\pm$ 6.6% and 81.0 $\pm$ 6.5%, for the period C, EEN and EED, respectively, indicating little significant difference among the periods. Taking above results into consideration, it seems the exercise program conducted for this study did not substantially affect the nitrogen balance of the obese children, and may be used far the body weight control. Howeever, the adequacy of the energy restriction and amount of exercise applied to this study need to be further investigated as the nitrogen balance indicated negative and increasing tendency of the creatinine excretion showed during the EED compared with period C, despite the statistical insignificance.

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Study on the Effects of the Extracts from Sophora Japonica L. Flowers, Fruits and Branches on Improvements in Skin Condition (회화나무 꽃, 열매, 가지 추출물의 피부개선 효과에 관한 연구)

  • Song, Hang Sun;Jang, Hye In
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.4
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    • pp.351-358
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    • 2016
  • The purpose of this research was to examine the effect of the ehanol extracts of Sophora japonica L. (S. japonica). flowers, fruits and branches on skin enhancement with assessing anti-oxidative, whitening, and wrinkle enhancement effects. Results showed that l,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities were $17.68{\pm}1.59{\sim}51.40{\pm}1.04$, $27.48{\pm}0.22{\sim}50.89{\pm}0.13$ and $30.79{\pm}0.55{\sim}45.17{\pm}0.83%$, respectively, in 50~1,000 mg/L of concentrations. The capacities of inhibiting tyrosinase of ethanol extracts from S. japonica. flowers, fruits and branches were $0.27{\pm}0.12{\sim}11.38{\pm}0.57$, $0.27{\pm}0.02{\sim}0.82{\pm}0.27$ and $0.09{\pm}0.16{\sim}0.55{\pm}0.27%$, respectively. The capacities of preventing porcine pancreatic elastase (PPE) were $3.70{\pm}1.23{\sim}7.28{\pm}1.01$, $3.06{\pm}2.13{\sim}13.03{\pm}2.99$ and $6.00{\pm}0.96{\sim}9.71{\pm}0.44%$, respectively, in the case of 50~1,000 mg/L of concentrations. It is concluded that the effects of S. japonica. flowers, fruits and branches on skin improvement are varied significantly.

The effects of sodium fluoride on oral normal cell cultured in vitro (체외에서 배양된 구강 내 정상세포에 불화나트륨이 미치는 영향)

  • Choi, Byul-Bora;Kim, Da-Hye;Kim, Ji-Young;Park, Sang-Rye
    • Journal of Korean society of Dental Hygiene
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    • v.16 no.3
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    • pp.471-477
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    • 2016
  • Objectives: Fluoride is widely used in the prevention and control of dental caries. The purpose of this study is to examine the biological effects of Sodium fluoride on the proliferation of oral normal cell in vitro(MDPC-23, HaCaT, HGF-1 cells). Methods: The proliferation of normal cells and the cyto-skeletal change of normal cells were assessed by WST-1 assay and F-actin stain assay. The statistical significances of the resulting data were analyzed using SPSS(Window 12.0). Results: The sodium fluoride(0-12 mM) treatment decreased the cell viability in a dose and time dependent manner: HaCaT(6 h): $100{\pm}0$, $98{\pm}0.39$, $82{\pm}2.68$, $75{\pm}0.83$, $69{\pm}1$, $67{\pm}1.42%$(p<0.005); HaCaT(24 h): $100{\pm}0$, $98{\pm}1.85$, $54{\pm}0.64$, $43{\pm}0.4$, $38{\pm}0.32$, $36{\pm}0.13%$(p<0.006), MDPC-23(6 h): $100{\pm}0$, $93{\pm}1.48$, $85{\pm}0.28$, $82{\pm}1.58$, $79{\pm}1.48$, $76{\pm}1.93%$(p<0.009); MDPC-23(24 h): $100{\pm}0$, $91{\pm}1.26$, $58{\pm}0.65$, $49{\pm}1$, $44{\pm}0.74$, $2{\pm}0.05%$(p<0.005), HGF-1(6 h): $100{\pm}0$, $97{\pm}2.93$, $89{\pm}5$, $71{\pm}5.42$, $58{\pm}4.82$, $43{\pm}3.47%$(p<0.009); HGF-1(24 h): $100{\pm}0$, $97{\pm}2.05$, $73{\pm}1.73$, $22{\pm}1.61$, $14{\pm}1.73$, $7{\pm}0.85%$(p<0.005). Thus, changes in cell morphology and disruption of filamentous(F)-actin organization were observed in higher concentration. Conclusions: These results suggest that higher concentrations of fluoride lead to a reduce the number of cells and morphology change of normal cell.

An Effectiveness of Simultaneous Measurement of PM10, PM2.5, and PM1.0 Concentrations in Asian Dust and Haze Monitoring

  • Cho, Changbum;Park, Gilun;Kim, Baekjo
    • Journal of Environmental Science International
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    • v.22 no.6
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    • pp.651-666
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    • 2013
  • This study introduces a novel approach to the differentiation of two phenomena, Asian Dust and haze, which are extremely difficult to distinguish based solely on comparisons of PM10 concentration, through use of the Optical Particle Counter (OPC), which simultaneously generates PM10, PM2.5 and PM1.0 concentration. In the case of Asian Dust, PM10 concentration rose to the exclusion of PM2.5 and PM1.0 concentration. The relative ratios of PM2.5 and PM1.0 concentration versus PM10 concentration were below 40%, which is consistent with the conclusion that Asian Dust, as a prime example of the coarse-particle phenomenon, only impacts PM10 concentration, not PM2.5 and PM1.0 concentration. In contrast, PM10, PM2.5 and PM1.0 concentration simultaneously increased with haze. The relative ratios of PM2.5 and PM1.0 concentration versus PM10 concentration were generally above 70%. In this case, PM1.0 concentration varies because a haze event consists of secondary aerosol in the fine-mode, and the relative ratios of PM10 and PM2.5 concentration remain intact as these values already subsume PM1.0 concentration. The sequential shift of the peaks in PM10, PM2.5 and PM1.0 concentrations also serve to individually track the transport of coarse-mode versus fine-mode aerosols. The distinction in the relative ratios of PM2.5 and PM1.0 concentration versus PM10 concentration in an Asian Dust versus a haze event, when collected on a national or global scale using OPC monitoring networks, provides realistic information on outbreaks and transport of Asian Dust and haze.

Comparison of Litter Production and Nutrient returned to Forest Floor according to Forest Fire Type and Recovery (산불유형과 회복정도에 따른 낙엽생산량과 임상으로 이입되는 영양염류 함량)

  • Kim, Jung-Sup;Yang, Keum-Chul
    • Korean Journal of Environment and Ecology
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    • v.26 no.1
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    • pp.67-73
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    • 2012
  • This study has compared the different types of forest fire sites and the unburned site at Samcheuk-si Gangwon-do by assessing the amount of litter production, nutrient concentration returned to forest floor from July 2007 through October 2010. The research showed that the average amounts of litterfall produced in the unburned site, the crown fire site(C-1), the crown fire site(C-3) and the ground fire site(G-2) were $7.74{\pm}2.56$, $1.17{\pm}0.67$, $2.97{\pm}0.44$ and $2.92{\pm}0.42ton{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively. In the unburned site, the average amounts of total nitogen(T-N), total phosphorus(T-P), potassium(K), calcium(Ca) and magnesium(Mg) returned to the forest floor were $22.20{\pm}12.43$, $1.16{\pm}0.46$, $2.68{\pm}1.44$, $16.22{\pm}4.69$ and $1.36{\pm}0.32 kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively whereas those in the crown fire site(C-1) were $3.73{\pm}2.31$, $0.10{\pm}0.09$, $0.27{\pm}0.20$, $2.75{\pm}2.58$ and $0.24{\pm}0.27 kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively. This study showed that the nutrient content returned to forest floor was higher in the following order: unburned site(Un) > crown fire site(C-3) > ground fire site(G-2) > crown fire site(C-1).

Quality Analysis of Diverse Rice Species for Rice Products (쌀 가공제품을 위한 다양한 쌀의 품질분석)

  • Kim, Hye-Ryun;Kwon, Young-Hee;Kim, Jae-Ho;Ahn, Byung-Hak
    • Korean Journal of Food Science and Technology
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    • v.43 no.2
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    • pp.142-148
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    • 2011
  • The objective of this study was to analyze proximate composition and physicochemical properties according to different kinds of rice. In total, 20 varieties of rice were used (Domestic-19, Imported-1). The moisture contents ranged from $11.11{\pm}0.20$ to $3.28{\pm}0.03%$. The crude protein and crude lipid contents were ranged from $5.04{\pm}0.03$ to $7.02{\pm}0.10%$ and $0.18{\pm}0.01$ to $0.73{\pm}0.05%$, respectively. The mineral contents were Calcium, $3.56{\pm}0.11-6.69{\pm}0.08mg/100g$; Sodium, $3.39{\pm}0.01-17.43{\pm}0.04mg/100g$; Phosphorus, $64.12{\pm}0.88-102.0{\pm}0.36mg/100g$; Zinc, $0.95{\pm}0.01-1.75{\pm}0.0mg/100g$; Iron, $0.19{\pm}0.0-0.69{\pm}0.02g/100g$; Magnesium, $9.89{\pm}0.47-23.31{\pm}0.21mg/100g$; Potassium, $47.11{\pm}3.49-82.19{\pm}1.08mg/100g$;and Manganese, $0.47{\pm}0.0-1.14{\pm}0.01mg/100g$. Eighteen kinds of rice exhibited small kernels. Amylose contents ranged from $10.3{\pm}1.27$ to $19.4{\pm}0.15%$, while starch value ranged from $70.8{\pm}2.67$ to $80.1{\pm}5.09%$. Alkali digestion value was described as 5-6 grade and gel consistency was shown to be 'soft' regardless of the rice kinds.

A Study of the Insulin and the C-Peptide Responses to Oral Glucose Load in Nondiabetic and Diabetic Subjects (정상인(正常人) 및 당뇨병환자(糖尿病患者)에서의 경구당부하시(經口糖負荷時) 혈중(血中) Insulin과 C-Peptide의 변동(變動))

  • Lee, Myung-Chul;Choi, Sung-Jae;Kim, Eung-Jin;Min, Hun-Ki;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.11 no.1
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    • pp.17-32
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    • 1977
  • The present study was undertaken to evaluate the significance of the insulin and the C-peptide rseponse to oral glucose loads in normal and diabetic subjects and to establish the effects of the obesity. In this study, the authors have measured plasma insulin and C-peptide by means of radioimmunoassay in 10 nonobese normal, 5 obese normal, 13 nonobese moderate diabetic patients, 9 obese moderate diabetic patients and 9 severe diabetic patients. The results obtained were as follows; 1. In 10 nonobese normal subjects, the plasma insulin level at fasting state and at 30, 60, 90, and 120 min after oral glucose loads were $15.7{\pm}3.4,\;48.3{\pm}9.8,\;40.4{\pm}6.7,\;37.4{\pm}6.5\;and\;26.0{\pm}4.2uU/ml(Mean{\pm}S.E.)$ and C-peptide were $1.9{\pm}0.3,\;3.9{\pm}0.6,\;6.3{\pm}0.6,\;5.7{\pm}0.5\;and\;4.0{\pm}0.5ng/ml$. The change of C-peptide was found to go almost parallel with that of insulin and the insulin value reaches to the highest level at 30 min whereas C-peptide reaches to its peak at 60min. 2. The plasma insulin level in 5 obese normal subjects were $38.9{\pm}12.3,\;59.5{\pm}12.3,\;59.2{\pm}17.1,\;56.1{\pm}20.0\;and\;48.4{\pm}17.2uU/ml$ and the C-peptide were $5.5{\pm}0.4,\;6.8{\pm}0.5,\;7.9{\pm}0.8,\;7.9{\pm}0.8\;and\;7.8{\pm}2.0ng/ml$. The insulin response appeared to be greater than nonobese normal subjects. 3. In 13 nonobese moderate diabetic patients, the plasma insulin levels were $27.1{\pm}4.9,\;44.1{\pm}6.0,\;37.3{\pm}6.6,\;35.5{\pm}8.1\;and\;34.7{\pm}10.7uU/ml$ and the C-peptide levels were $2.7{\pm}0.4,\;4.9{\pm}0.7,\;6.5{\pm}0.5,\;7.0{\pm}0.3\;and\;6.7{\pm}1.0ng/ml$. There was little significance compared to nonobese normal groups but delayed pattern is noted. 4. In 9 obese moderated diabetic patients, the plasma insulin levels were $22.1{\pm}7.9,\;80.0{\pm}19.3,\;108.0{\pm}27.0,\;62.0{\pm}17.6\;and\;55.5{\pm}10.1uU/ml$ and the C-peptide levels were $5.2{\pm}0.4,\;8.0{\pm}1.0,\;10.4{\pm}1.6,\;10.4{\pm}1.7\;and\;10.1{\pm}1.0ng/ml$ and its response was also greater than that of nonobese moderate diabetic patients. 5. The plasma insulin concentrations in 9 severe diabetic subjects were $8.0{\pm}3.8,\;12.1{\pm}3.5,\;16.8{\pm}4.6,\;19.6{\pm}5.2\;and\;15.0{\pm}5.0uU/ml$ and the C-peptide levels were $1.6{\pm}0.3,\;2.4{\pm}0.4,\;4.1{\pm}0.6,\;4.0{\pm}0.8\;and\;4.5{\pm}0.7ng/ml$ and the insulin and C-peptide responses were markedly reduced in severe diabetic groups. 6. There were-significant differences between each groups of patients on the magnitude of total insulin or C-peptide areas, the insulinogenic index and the C-peptide index.

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