• Clinical and Experimental Reproductive Medicine
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• 제27권1호
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• pp.47-57
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• 2000

Objective: The effects of cryopreservation with or without coculture on the in vitro development of blastomere-biopsied 8-cell mouse embryos were investigated. This experimental study was originally designed for the setup of a preclinical mouse model for the preimplantation genetic diagnosis (PGD) in human. Methods: Eight-cell embryos were obtained after in vitro fertilization (IVF) from F1 hybrid mice (C57BL(표현불가)/CBA(표현불가)). Using micromanipulation, one to four blastomeres were aspirated through a hole made in the zona pellucida by zona drilling (ZD) with acid Tyrode's solution (ATS). A slow-freezing and rapid-thawing protocol with 1.5M dimethyl sulfoxide (DMSO) and 0.1M sucrose as cryoprotectant was used for the cryopreservation of blastomere- biopsied 8-cell mouse embryos. After thawing, embryos were cultured for 110 hours in Ham's F-10 supplemented with 0.4% bovine serum albumin (BSA). In the coculture group, embryos were cultured for 110 hours on the monolayer of Vero cells in the same medium. The blastocyst formation was recorded, and the embryos developed beyond blastocyst stage were stained with 10% Giemsa to count the total number of nuclei in each embryo. Results: The survival rate of embryos after cryopreservation was significantly lower in the blastomere-biopsied (7/8, 6/8, 5/8, and 4/8 embryos) groups than in the non-biopsied, zona intact (ZI) group. Without the coculture, the blastocyst formation rate of embryos after cryopreservation was not significantly different among ZI, the zona drilling only (ZD), and the balstomere-biopsied groups, but it was significantly lower than in the non-cryopreserved control group. The mean number of cells in embryos beyond blastocyst stage was significantly higher in the control group ($50.2{\pm}14.0$) than in 6/8 ($26.5{\pm}6.2$), 5/8 ($25.0{\pm}5.5$), and 4/8 ($17.8{\pm}7.8$) groups. With the coculture using Vero cells, the blastocyst formation rate of embryos after cryopreservation was significantly lower in 5/8 and 4/8 groups, compared with the control, 7/8, and 6/8 groups. The mean number of cells in embryos beyond blastocyst stage was also significantly lower in 4/8 group ($25.9{\pm}10.2$), compared with the control ($50.2{\pm}14.0$), 7/8 ($56.0{\pm}22.2$), and 6/8 ($55.3{\pm}25.5$) groups. Conclusion: After cryopreservation, blastomere-biopsied mouse embryos have a significantly impaired developmental competence in vitro, but this detrimental effect might be prevented by the coculture with Vero cells in 8-cell mouse embryos biopsied one or two blastomeres. Biopsy of mouse embryos after ZD with ATS is a safe and highly efficient preclinical model for PGD of human embryos.

• The Korean Journal of Physiology and Pharmacology
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• 제26권6호
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• pp.405-413
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• 2022

Hair loss is a common status found among people of all ages. Since the role of hair is much more related to culture and individual identity, hair loss can have a great influence on well-being and quality of life. It is a disorder that is observed in only scalp patients with androgenetic alopecia (AGA) or alopecia areata caused by stress or immune response abnormalities. Food and Drug Administration (FDA)-approved therapeutic medicines such as finasteride, and minoxidil improve hair loss temporarily, but when they stop, they have a limitation in that hair loss occurs again. As an alternative strategy for improving hair growth, many studies reported that there is a relationship between the expression levels of prostaglandins (PGs) and hair growth. Four major PGs such as prostaglandin D2 (PGD2₂), prostaglandin I2 (PGI₂), prostaglandin E2 (PGE₂), and prostaglandin F2 alpha (PGF_2α) are spatiotemporally expressed in hair follicles and are implicated in hair loss. This review investigated the physiological roles and pharmacological interventions of the PGs in the pathogenesis of hair loss and provided these novel insights for clinical therapeutics for patients suffering from alopecia.

• 정보보호학회논문지
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• 제31권6호
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• pp.1215-1225
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• 2021

인공지능 기술은 우수한 성능을 기반으로 다양한 분야에 적용되고 있지만 입력 데이터에 인간이 감지할 수 없는 적대적 섭동을 추가하여 인공지능 모델의 오작동을 유도하는 적대적 예제에 취약하다. 현재까지 적대적 예제에 대응하기 위한 방법은 세 가지 범주로 분류할 수 있다. (1) 모델 재학습 방법; (2) 입력 변환 방법; (3) 적대적 예제 탐지 방법. 이러한 적대적 예제에 대응하기 위한 방법은 끊임없이 등장하고 있지만 각 적대적 공격 유형을 분류하는 연구는 미비한 실정이다. 따라서, 본 논문에서는 차원 축소와 군집화 알고리즘을 활용한 적대적 공격 유형 분류 방법을 제안한다. 구체적으로, 제안하는 방법은 적대적 예시로부터 적대적 섭동을 추출하고 선형 판별 분석(LDA)를 통해 적대적 섭동의 차원을 축소한 후에 k-means 알고리즘으로 적대적 공격 유형 분류를 수행한다. MNIST 데이터셋과 CIFAR-10 데이터셋을 대상으로 한 실험을 통해, 제안하는 기법은 5개의 적대적 공격(FGSM, BIM, PGD, DeepFool, C&W)을 효율적으로 분류할 수 있으며, 적대적 예제에 대한 정상 입력을 알 수 없는 제한적인 상황에서도 우수한 분류 성능을 나타내는 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제8권4호
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• pp.357-364
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• 1995

A biochemical genetic study on blood enzyme/protein systems in some breeds/crosses of sheep in Malaysia was carried out using horizontal starch gel electrophoresis. Blood samples were collected from 435 sheep, representing 8 breeds/crosses. These included 5 wool sheep breeds (Thai Longtail, wiltshire, Suffolk, Dorsimal and cMBLx), 1 hair sheep breed (Barbados Blackbelly) and 2 hybrids between wool sheep and hair sheep (Cameroon ${\times}$ Thai Longtail and Bali Bali ${\times}$ Malin). Twenty loci systems were examined. Of these, ten ($HB{\beta}$, ALB, TF, XP, CAT, DIA1, EsA, GPI, ME and NP) exhibited genetic variation whereas the other ten (AAT, CA, DIA2, ${\alpha}GLO$, ${\alpha}GLU$, LDH, MDH, PEP[leu-gly-gly], 6PGD and SOD) were monomorphic. The allelic frequencies which were obtained in 10 polymorphic markers are assessed and compared with the results obtained by previous workers. The estimations of inbreeding coefficient, intrabreed variation and breed relationships have been critically discussed and are used to reveal some important recommendations.

• Coupled systems mechanics
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• 제5권2호
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• pp.101-126
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• 2016

Seismic structural fragility constitutes an important step for performance based seismic design. Lateral load-resisting structural members are often analyzed under one component base excitation, while the effect of bi-directional shaking is accounted per simplified rules. Fragility curves are constructed herein under real bi-directional excitation by a simple extension of the conventional Incremental Dynamic Analysis (IDA) under uni-directional shaking. Simple SODF systems, parametrically adjusted to different periods, are examined under a set of near-fault and far-fault excitations. Consideration of bi-directional interaction appears important for stiff systems. Further, the study indicates that the peak ground accelertaion, velocity and displacement (PGA, PGV and PGD) of accelerogram are relatively stable and efficient intensity measures for short, medium and long period systems respectively. '30%' combination rule seems to reasonably predict the fragility under bi-directional shaking at least for first mode dominated systems dealt herein up to a limit state of damage control.

• 한국임상보건과학회지
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• 제2권1호
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• pp.17-23
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• 2014

Purpose. This study was to investigate the relationship between the palpebral fissure size and the exophthalmos in a population of Korean adult women subjects with normal eye status. Methods. From November 2012 to September 2013, The authors analyzed the morphological values in 73 Korean adults population. The test was performed using the Hertel exophthalmometer and verner califer. Analysis of subjects data were recorded using the spss version 20.0 a statistical program (IBM Co, Armonk, NY USA). Results. The average age of the 73 subjects (146 eyes) was (21.219+/-0.261)mm. the average value of palpebral fissure was (25.110+/-1.646)mm in width and (8.096+/-1.464)mm in height. The average value of exophthalmos was (15.002+/-1.922)mm. There was a significant relationship(P>0.05) in the average size between the width and height in palpebral fissure. Also, there was a significant relationship(P>0.05) in the average size between the palpebral fissure width and exophthalmos. On the other hand, there was a significant relationship(P>0.01) in the average size of palpebral fissure height and exophthalmos. Conclusions: In this study, these results suggested that the palpebral fissure and exophthalmos had a significant relationship in the average value of size in Korean adult women population.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.27-35
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• 1993

In an attempt to reveal the interrelationship between fowls of jungle and native origin, their gene constitutions were compared using gene frequencies at the 16 loci controlling blood protein variations. Of the 16 loci analysed by electrophoresis, polymorphism was detected at following seven loci: Es-1, Amy-1, Akp-akp, Akp-2, Alb, Tf and 6-PGD. The other nine loci: Amy-3, Es-D, PGM, PHI, MDH, To, LDH, Hb-1 and Hb-2, were noted to be monomorphic. Genetic distance between pairs of native fowl and jungle fowls was estimated by a numerical taxonomic method. The Indonesian native fowl was genetically close to the Indonesian red jungle fowl, and the grey jungle fowl was genetically similar to the Ceylonese jungle fowl. It was also suggested that the green jungle fowl was genetically remote from the other jungle fowls and from the Indonesian native flow. The proportion of polymorphic loci (Ppoly), the expected average heterozygosity per individual $\bar{H}$, and the effective number of alleles per locus (Ne) were calculated to evaluate the genetic variabilities in the native and jungle fowls. The Indonesian native fowl exhibited slightly higher the proportion of polymorphic loci than the jungle fowls.

• Geomechanics and Engineering
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• 제37권2호
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• pp.189-195
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• 2024

Buried pipelines can be classified as continuous and segmented pipelines. These infrastructures can be damaged either by ground movement or by seismic wave propagation during an earthquake. Permanent ground deformations (PGD) include surface faulting, liquefaction-induced lateral spreading and landslide. Liquefaction is a major problem for both superstructures and infrastructures. Buyukcekmece lake zone, which is the studied region in this paper, is a liquefaction prone area located near the North Anatolian Fault Line. It is an active fault line in Turkey and a major earthquake with a magnitude of around 7.5 is expected in this investigated region in Istanbul. It is planned to be constructed a new 12" steel natural gas pipeline from one side of the lake to the other side. In this study, this case has been examined in terms of two different support conditions. Firstly, it has been defined as a beam in liquefied soil and has built-in supports at both ends. In the other approach, this case has been modeled as a beam in liquefied soil and has vertical elastic pinned supports at both ends. These models have been examined and some solution proposals have been produced according to the obtained results. In this study, based on this sample, it is aimed to determine the behaviors of buried continuous pipelines subject to liquefaction effects in terms of buoyancy.

• 대한약침학회지
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• 제5권2호
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• pp.40-51
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• 2002

Objectives : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide-induced expression phospholipase $A_2$, cyclooxygenase-2 and inducible nitrogen oxide synthase, and the generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells, a murine macrophage cell line. Methods : The expression of phospholipase $A_2$, cyclooxygenase and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was assayed by ELISA method in RAW 264.7 cells. The non-toxic concentrations (0.1 to $5\;{\mu}g/ml$) of bee venom determined by MTT assay, were used in this study. Results : 1. Bee venom inhibited lipopolysaccharide-induced expression of phospholipase $A_2$ in a dose dependent manner after 48 hours treatment. 2. Bee venom inhibited lipopolysaccharide-induced expression of cyclooxygenase-2 in a dose dependent manner after 24 and 48 hours treatment. 3. Bee venom inhibited lipopolysaccharide-induced expression of inducible nitrogen oxidesynthase in a dose dependent manner after 48 hours treatment. 4. The generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was not much affected by the treatment of bee venom on the lipopolysaccharide-induced generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ in RAW 264.7 cells.

• Archives of Pharmacal Research
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• 제26권10호
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• pp.832-839
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• 2003

Activated macrophages express inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase (COX-2), and produce excessive amounts of nitric oxide (NO) and prostaglandin E$_2$ (PGE$_2$), which play key roles in the processes of inflammation and carcinogenesis. The root of Paeonia lactiflora Pall., and the root cortex of Paeonia suffruticosa Andr., are important Chinese crude drugs used in many traditional prescriptions. 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose (PGG) is a major bioactive constituent of both crude drugs. PGG has been shown to possess potent anti-oxidant, anti-mutagenic, anti-proliferative and anti-invasive effects. In this study, we examined the inhibitory effects of 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose (PGG) isolated from the root of Paeonia lactiflora Pall. on the COX-2 and iNOS activity in LPS-activated Raw 264.7 cells, COX-1 in HEL cells. To investigate the structure-activity relationships of gallate and gallic acid for the inhibition of iNOS and COX-2 activity, we also examined (-)-epigallocatechin gallate (EGCG), gallic acid, and gallacetophenone. The results of the present study indicated that PGG, EGCG, and gallacetophenone treatment except gallic acid significantly inhibited LPS-induced NO production in LPS-activated macrophages. All of the four compounds significantly inhibited COX-2 activity in LPS-activated macrophages. Among the four compounds examined, PGG revealed the most potent in both iNOS ($IC_{50}$ = 18 $\mu\textrm{g}/mL$) and COX-2 inhibitory activity (PGE$_2$: $IC_{50}$ = 8 $\mu\textrm{g}/mL$ and PGD$_2$: $IC_{50}$ = 12 $\mu\textrm{g}/mL$), respectively. Although further studies are needed to elucidate the molecular mechanisms and structure-activity relationship by which PGG exerts its inhibitory actions, our results suggest that PGG might be a candidate for developing anti-inflammatory and cancer chemopreventive agents.