Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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The Effects of Bee Venom on PLA2, COX-2, iNOS, AA and PG in RAW 264.7 Cells

봉약침액(蜂藥鍼液)이 PLA2, COX-2, iNOS, AA 및 미치는 영향(影響)에 관(關)한 연구(硏究)

  • Ha, Seang-Jong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University) ;
  • Lee, Seong-No (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University) ;
  • Jo, Hyun-Chul (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-Won University) ;
  • Kim, Kee-Hyun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University)
  • 하성종 (경원대학교 한의과대학 침구학교실) ;
  • 이성노 (경원대학교 한의과대학 침구학교실) ;
  • 조현철 (경원대학교 한의과 대학 한방재활의학과 교실) ;
  • 김기현 (경원대학교 한의과대학 침구학교실)
  • Published : 2002.12.01
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Abstract

Objectives : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide-induced expression phospholipase $A_2$, cyclooxygenase-2 and inducible nitrogen oxide synthase, and the generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells, a murine macrophage cell line. Methods : The expression of phospholipase $A_2$, cyclooxygenase and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was assayed by ELISA method in RAW 264.7 cells. The non-toxic concentrations (0.1 to $5\;{\mu}g/ml$) of bee venom determined by MTT assay, were used in this study. Results : 1. Bee venom inhibited lipopolysaccharide-induced expression of phospholipase $A_2$ in a dose dependent manner after 48 hours treatment. 2. Bee venom inhibited lipopolysaccharide-induced expression of cyclooxygenase-2 in a dose dependent manner after 24 and 48 hours treatment. 3. Bee venom inhibited lipopolysaccharide-induced expression of inducible nitrogen oxidesynthase in a dose dependent manner after 48 hours treatment. 4. The generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ was not much affected by the treatment of bee venom on the lipopolysaccharide-induced generation of arachidonic acid, prostaglandin $D_2$ and $E_2$ in RAW 264.7 cells.

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References

  1. 蜂毒療法과 蜂針療法 김문호
  2. 大韓鍼灸學會誌 v.11 no.1 蜂針에 대한 考察 권기록;고형균;김창환
  3. 大韓韓醫學會誌 v.13 no.1 蜂毒鍼療法이 抗炎, 鎭痛 및 解熱에 미치는 效能에 관한 實驗的 硏究 고형균
  4. 大韓鍼灸學會誌 v.12 no.1 蜂毒療法이 흰쥐의 膝關節 炎症性에 미치는 影響 도원석;장준혁;김경호(외 2인)
  5. 大韓鍼灸學會誌 v.15 no.2 蜂毒藥鍼療法이 抗炎, 鎭通作用에 미치는 效能에 관한 實驗的 硏究 권기록;고형균
  6. 大韓鍼灸學會誌 v.15 no.1 蜂毒藥鍼療法의 抗炎症 作用에 관한 實驗的 硏究 김지영;고형균;김용섭(외 3인)
  7. 大韓鍼灸學會誌 v.18 no.1 柳根皮 桂枝 牛膝 蜂毒 및 牛黃 態膽 麝香 復合製制 藥鍼이 mouse의 LPS 銹發 關節炎의 血液學的 變化에 미치는 影響 도원석;김경호;김갑성
  8. 大韓鍼灸學會誌 v.10 no.1 蜂針毒療法이 抗痙攣에 미치는 影響 공현숙;고형균;김창환
  9. 大韓鍼灸學會誌 v.14 no.2 蜂毒藥鍼刺戟이 3-MCA 誘發 上皮腫에 대한 抗癌 및 免疫反應에 미치는 影向 권기록;고형균;김창환(외 3인)
  10. 大韓藥鍼學會誌 v.3 no.1 蜂毒藥鍼의 抗癌 效果에 대한 分子生物學的 硏究 박찬열;서정철;최도영(외 1인) https://doi.org/10.3831/KPI.2000.3.1.001
  11. 藥鍼用 蜂毒成分 中 apamin의 抗癌效果와 MAP-Kinase 神毫傳達體系에 대한 硏究 v.18 no.4 김윤미;이재동;박동석
  12. 大韓鍼灸學會誌 v.18 no.1 藥鍼用 蜂毒成分 中 apamin, Melittin의 抗癌作用
  13. 大韓鍼灸學會誌 v.19 no.4 蜂毒藥鍼이 C57BL mouse의 黑色腫에 미치는 影響 오기남;이재동;박동석
  14. Endocrinology v.121 no.5 Inhibition by Melittin and fluphenazine of melanotropin receptor function and adenylate cyclase in M2R melanoma cell membranes Gerst, J.E.;Salomon, Y. https://doi.org/10.1210/endo-121-5-1766
  15. 大韓鍼灸學會誌 v.17 no.1 蜂藥鍼療法의 免疫反應에 관한 臨床的 硏究 권기록;고형균
  16. 大韓鍼灸學會誌 v.16 no.3 牛膝 및 蜂毒藥鍼 이 생쥐의 LPS 誘發 關節炎의 細朐性 免疫反應에 미치는 影響 이승덕;김갑성
  17. 大韓鍼灸學會誌 v.17 no.4 山地別 蜂毒液藥鍼刺戟이 免疫機能低下에 미치는 影響 이경희;김창환;강성길(외 1인)
  18. 大韓鍼灸學會誌 v.18 no.3 退行性 膝關節炎의 蜂毒藥鍼 治療效果에 대한 臨床的 硏究 왕오호;안규범;임진강(외 1인)
  19. 大韓鍼灸學會誌 v.16 no.3 膝關節炎에 대한 蜂毒藥鍼의 臨床的 考察 김지훈;이재동
  20. 大韓鍼灸學會誌 v.18 no.5 蜂藥鍼을 이용한 류마토이드 關節炎의 臨床的 硏究 황유진;이건목;황우준(외 5인)
  21. 大韓鍼灸學會誌 v.19 no.4 蜂毒藥鍼이 제2형 콜라겐 誘導 關節炎에 미치는 影響 김태우;최도영;이재동
  22. 暻園大學校 韓醫科大學 碩士學位論文 蜂藥鍼이 實驗的 急慢性 關節炎에 미치는 效果와 機轉 硏究 이재웅
  23. 大韓鍼灸學會誌 v.19 no.4 蜂毒 및 Melittin 藥鍼液이 關節炎 滑液細구에 미치는 影響 한상원;박기현;정태영(외 1인)
  24. 大韓鍼灸學會誌 v.19 no.4 國産蜂毒 및 精製蜂毒藥鍼液이 류머틱스 關節炎 滑液細구에 미치는 影響 이윤섭;서정철;이승우(외 1인)
  25. 大韓鍼灸學會誌 v.19 no.4 蜂毒 藥鍼液의 細구毒性에 관한 硏究 박원;김용석;고형균
  26. 大韓鍼灸學會誌 v.11 no.1 藥鍼用 蜂毒液의 急性毒性에 관한 硏究 이종석;고형균;김창환
  27. 大韓鍼灸學會誌 v.16 no.1 藥鍼用 蜂毒液의 局所毒性試驗에 관한 硏究 이종석;고형균;김창환
  28. 免疫學 김세종
  29. Biochim Biophys Acta v.1170 no.3 Mammalian non-pancreatic phospholipases A$_2$ Kudo, I.;Murakami, M.;Hara, S.;Inoue, K. https://doi.org/10.1016/0005-2760(93)90003-R
  30. Prostaglandins v.53 no.3 Phospholipase A$_2$ in human placental serum Buhl, W.J.;Eisenlohr, L.M.;Gehring, U. https://doi.org/10.1016/S0090-6980(97)00012-9
  31. FASWB J. v.12 no.12 Cyclooxygenase in biology and disease Dubios, R.N.;Abramson, S.B.;Crofford, L.;Gupta, R.A.;Simon, L.S.;Putte, Van De;Lipsty, P.E.
  32. J. Immunol v.165 Capsaicin inhibits platelet activating factor-induced cytosolic Ca2+ increase and superoxide production Choi, S.Y.;Ha, H.;Kim, K.T. https://doi.org/10.4049/jimmunol.165.7.3992
  33. Prostaglandins Other Lipid Mediat v.56 no.5-6 The advent of highly selective inhibitors of cyclooxygenase-a review Cryer, B.;Dubois, A. https://doi.org/10.1016/S0090-6980(98)00064-1
  34. Anal Biochem. v.271 no.1 Intracellular measerment of prostaglandin E2: effect of anti-inflammatory drugs on cycloozygenase activity and prostanoid expression Horton, J.K.;Williams, A.S.;Smith-Phillips, Z.;Martin, R.C.;O'Beirne, G. https://doi.org/10.1006/abio.1999.4118
  35. Inpharma no.1122 Selective COX-2 inhibitors gather speed Cameron, A.
  36. 大韓鍼灸學會誌 v.15 no.1 蜂毒療法에 대한 韓醫學 最初의 文獻紀錄:마왕퇴이서의 蜂毒療法2列 인창식;고형균
  37. 鍼灸治療學 임종국
  38. 鍼灸學 上海中醫學院
  39. In Venomous Animals and their Venoms v.3 Chemistry, pharmacology and toxicology of bee, wasp and hornet venoms Habermann, E.
  40. brit. Pharmacol Histamine release by MCDP (401), A epetide from the venom of the honey bee Assem, E.S.;Atkinson, G.
  41. Biochem Biophys Res commun v.238 no.2 Melittin binds to secretory phospholipase A$_2$ and inhibits its enzymatic activity saini, S.S.;Peterson, J.W.;Chopra, A.K. https://doi.org/10.1006/bbrc.1997.7295
  42. Can Med Assoc J. v.155 no.1 An evidence-based approach to prescirbing NSAIDs in musculoskeletal disease: A canandian consensus Tannenbaum, H.;Davis, P.;Russell, A.S.
  43. Prostaglandins v.54 no.3 Cyclooxygenases and the central nervous system Kaufmann, W.E.;Andreasson, K.I.;Isakson, P.C.;Worley, P.F. https://doi.org/10.1016/S0090-6980(97)00128-7
  44. Biochimica et Bioghysica Acta. v.1568 Inhibitory effects of phloroglucinol derivatives from Mallotus japonicus on nitric oxide production by a murine macrophage-like cell line, RAW 264.7, activatied by lipopolysacchaide and interferon-${\alpha}$ https://doi.org/10.1016/S0304-4165(01)00203-3