• Journal of Korean Society of Forest Science
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• v.72 no.1
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• pp.27-31
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• 1986

To compare the fungal distribution of matsutake-producing (Tricholoma matsutake) stand with that of non-producing stand, mushrooms on forest floor were collected in September of 1984 from 28-year-old non-producing and 55-year-old matsutake-producing stands of Pinus densiflora in Kangwon Province. A total of 18 fungal species in 12 genera were collected from a non-producing stand, and all the species except one were putative ectomycorrhizal. Common genera in non-producing stand included Suillus, Russula, Lactarius, Hydnum, and Amanita. From a matsutake-producing stand a total of 30 fungal species in 22 genera were collected, of which 22 species were ectomycorrhizal. Common genera in the producing stand included Hydnum, Tricholoma, Laccaria, and Cantharellus. Common species in both stands were six species only. It is concluded that matsutake-producing stand had more diversified fungal population than non-producing stand, probably because of higher contents of organic matters in soil and better development of understory vegetation through the ageing of the stand.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.3
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• pp.276-285
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• 1986

This study was undertaken to investigate the characteristics of the proteolytic enzyme extracted from Neungee mushroom [Sarcodon aspratus (Berk.) S. Ito]. The enzyme was purified by using Tris-acryl CM-cellulose ion exchange, gel filtration on Ultrogel AcA 54, Hydroxy apatite column chromatography and preparative isoelectic focusing. The specific activity of the purified enzyme increased 8 times as compared with that of the crude enzyme. The enzyme was homogeneous on polyacrylamide gel electrophoresis (PAGE). The optimum pH was 10.1, indicating the enzyme to be alkaline protease and the optimum temperature was $57^{\circ}C$. The enzyme was stable at temperatures lower than $50^{\circ}C$and at pH values ranging from 4.0 to 10.8. However, the enzyme activity decreased by 26 and 65% at 60 and $65^{\circ}C$, respectively, when incubated for 30 minutes. The enzyme activity was activated by $Mn^{++}$ and inhibited by $Cu^{++}$ and $Hg^{++}$. The enzyme was consisted of monomer and its molecular weight estimated to be about 30,100 when determined by sodium dodecyl sulfate PAGE. Isoelectric point of the enzyme was determined to be 9.80.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.7 no.1
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• pp.29-37
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• 2013

The purpose of this study is to suggest the potential domestic and international market conditions vehicles for people with disabilities through a review of the literature and internet resources, and to propose a road map developing South Korean vehicles for people with disabilities taking into consideration the current state of domestic and international technology and the usage of the vehicles for people with disabilities depending on the type of disability. The results of the study are as follows: First, vehicles for people with disabilities safety standards of South Korea do not meet the safety standards of developed countries and the technology of development related vehicles for people with disabilities is not as advanced as that of developed countries. Vehicles for people with disabilities installation methods, specifications, features and safety standards should be designed to be more similar to those of Japan and the United States. Second, vehicle modifications for people with disabilities will become more common as measures directed at promoting the welfare of people with disabilities, such as travel and transportation measures, employment opportunities, education, and funding for vehicle modifications, are implemented along with laws, such as The Americans with Disabilities Act, The British Disability Discrimination Act, and Japan's barrier-free transport law, that protect people with disabilities generally. Third, through a comparative analysis of domestic and international market conditions and demands of people with disabilities in vehicle modifications, important things to pay attention by purchasing a modified vehicle for people with disabilities are the employment rate for people with disabilities and monthly income. To enable this, government agencies and industry bodies should work together through the revision of the current Disability Welfare Act or the enactment of the special law for guiding principles of Disability Welfare Act. Through the future changes in vehicles for people with disabilities-related system or society of South Korean, the annual demand of the vehicles is expected to be more than 10,000.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.39
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• pp.26.1-26.10
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• 2017

Background: The impairment of the appearance is a major problem for patients with carcinomas of the oral cavity. These patients want to recover their preoperative facial appearance. Some do not realize that this is not always possible and hence develop a desire for further cosmetic and reconstructive surgery (CRS) which often causes psychological problems. Method: The desire of patients for CRS (N = 410; 26%) has been acquired in this $D{\ddot{O}}SAK$ rehab study including multiple reasons such as medical, functional, aesthetic and psychosocial aspects. They relate to the parameters of diagnosis, treatment and postoperative rehabilitation. Patients without the wish for CRS (N = 1155; 74%) served as control group. For the surgeons, knowledge of the patient's views is relevant in the wish for CRS. Nevertheless, it has hardly been investigated for patients postoperatively to complete resection of oral cancer. In this retrospective cross-sectional study, questionnaires with 147 variables were completed during control appointments. Thirty-eight departments of Oral and Maxillofacial Surgery took part, and 1652 German patients at least 6 months after complete cancer resection answered the questions. Additionally, a physician's questionnaire (N = 1489) was available. Statistical analysis was performed with SPSS vers. 22. Results: The patient's assessment of their appearance and scarring are the most important criteria resulting in wishes for CRS. Furthermore, functional limitations such as eating/swallowing, pain of the facial muscles, numb regions in the operating field, dealing with the social environment, return to work, tumour size and location, removal and reconstruction are closely related. Conclusion: The wish for CRS depends on diverse functional psychosocial and psychological parameters. Hence, it has to be issued during conversation to improve rehabilitation. A decision on the medical treatment can be of greater satisfaction if the surgeon knows the patients' needs and is able to compare them with the medical capabilities. The informed consent between doctor and patient in regard to these findings is necessary.

• Journal of Ginseng Research
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• v.41 no.1
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• pp.103-112
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• 2017

Background: 20(S)-Protopanaxadiol 20-O-D-glucopyranoside, also called compound K (CK), exerts antidiabetic effects that are mediated by insulin secretion through adenosine triphosphate (ATP)-sensitive potassium ($K_{ATP}$) channels in pancreatic ${\beta}$-cells. However, the antidiabetic effects of CK may be limited because of its low bioavailability. Methods: In this study, we aimed to enhance the antidiabetic activity and lower the toxicity of CK by including it with ${\beta}$-cyclodextrin (CD) (CD-CK), and to determine whether the CD-CK compound enhanced pancreatic islet recovery, compared to CK alone, in an alloxan-induced diabetic zebrafish model. Furthermore, we confirmed the toxicity of CD-CK relative to CK alone by morphological changes, mitochondrial damage, and TdT-UTP nick end labeling (TUNEL) assays, and determined the ratio between the toxic and therapeutic dose for both compounds to verify the relative safety of CK and CD-CK. Results: The CD-CK conjugate ($EC_{50}=2.158{\mu}M$) enhanced the recovery of pancreatic islets, compared to CK alone ($EC_{50}=7.221{\mu}M$), as assessed in alloxan-induced diabetic zebrafish larvae. In addition, CD-CK ($LC_{50} =20.68{\mu}M$) was less toxic than CK alone ($LC_{50}=14.24{\mu}M$). The therapeutic index of CK and CD-CK was 1.98 and 9.58, respectively. Conclusion: The CD-CK inclusion complex enhanced the recovery of damaged pancreatic islets in diabetic zebrafish. The CD-CK inclusion complex has potential as an effective antidiabetic efficacy with lower toxicity.

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.177-181
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• 1983

To improve cultivation technique for the stable and high yield, the outdoor fermentation of rice straw bundles, and pasteurization and fermentation of rice straw compost in tunnel were carried out. 1) Mycelial growth of P. ostreatus was becoming rapid and dense according to prolongation of fermentation periods outdoor rice straw bundles, but days untill fully mycelial growth after spawning was short. 2) As the fermentation period of rice straw bundles was 6 days (three times turning), yield of P. ostreatus was the highest as $50.5kg/3.3m^2 and increased 24% of yield than those of conventional method. 3) When it was fermented rice straw compost in tunnel for 4 days, mycelial growth of P. ostreatus was rapid and dense, and also days untill budding after spawning, was shorter, yield $67.6kg/3.3m^2$, resulted in increasing 17% of yield than those of conventional method. 4) On being sterilization and fermentation of the rice straw compost in tunnel, the production cost was decreased 22.3% in compared with conventional method.

• Journal of the Korean Society of Clothing and Textiles
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• v.26 no.5
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• pp.630-641
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• 2002

The purpose of this study is to suggest the standard of bodytype which offers various data with regard to three-dimensional characteristics of body, and which offers the criterion for the sizes and shapes to interpret various bodytypes, to estimate filling, and to construct ready-made clothing. In this study, standard bodytype was defined as a typical type with beautiful proportion and shape which a large number of people have, too. In order to continue this study, there were two ways of analyzing bodytypes of subjects in the view of statistics and beauty. We primarily carried on statistical analysis in this report. Taken as a sample, the subjects were ninety-seven females who were between the ages of 18 and 24 years o1d. They were measured on November 1999. The data of seven hundred and few females, 18∼24 year-old, from the National Somatometry Survey of 1997 were used for analysis, too. Three kinds of classification methods were used for statistical analysis. The first method was based on the lateral view of the body, the second was based on the Factor and Cluster analysis of the photographic measurements of each of the anterior and lateral body, and the third was based on the Factor and Cluster analysis of the anthropometric measurements of the whole body. From the analysis on the distribution of R hrer Index the difference of width, the difference of depth, the proportion of height and head height, the High-frequency Proportion(H.P.)range were selected. The straight type from the lateral view, High-frequency type from each method, and subjects in H.P. range were compared with each other. As the result, it was confirmed that it is proper to base on the size and shape of subjects in H.P. range to analyze the standard bodytype.

• The Korean Journal of Pesticide Science
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• v.5 no.2
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• pp.1-12
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• 2001

A competitive indirect enzyme-linked immunosorbent assay (ci ELISA) for the organophosphorus insecticide acephate, O,S-dimethyl acetylphosphoramidothioate, was developed using a polyclonal antibody. Three different haptens mimicking the analyze and containing hexanoic acid moiety as a linker were synthesized, and then conjugated with the carrier proteins bovine serum albumin and keyhole limpet hemocyanin by the N-hydroxysuccinimide active ester method. Polyclonal antibodies raised against hapten-KLH conjugates in rabbits and the hapten-BSA conjugates as coating antigens were screened and selected for the assay in the homologous and/or heterologous ELISA system. The effects of various assay conditions, including blocking reagents, detergent content, organic solvents, pH, and preincubation of tile mixture of the polyclonal antibody and the analyze on the sensitivity were evaluated. The $IC_{50}$ value of acephate of 110 ng/mL was obtained in an optimized heterologous system using hapten-3-BSA as a coating antigen and a polyclonal antibody 8377, showing the detection range of 10-1000 ng/mL and the lowest detection limit of 4 ng/mL. The cross-reactivities of the structurally related insecticides, including methamidophos were less than 0.02%. These results indicate that the ELISA could be a convenient and alternative tool for monitoring acephate residues in agricultural products and environmental samples.

• Journal of Animal Environmental Science
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• v.18 no.sup
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• pp.29-34
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• 2012

This study was conducted to investigate the effect of storage time of manure on the concentration of odorous compounds. Levels of odorous compounds were measured from manure incubated in $20^{\circ}C$ for 6 wk in pilot chamber whose structure is similar to slurry pit. Levels of short chain fatty acids were decreased (p<0.05) by 4,159, 1,925, 844, and 483 ppm as storage time increased as 0, 2, 4, and 6wk, respectively. Transfatty acid level was not changed for 2wk but decreased (p<0.05) afterwards (levels were 250, 248, 151, and 61 ppm at 0, 2, 4, 6wk, respectively). Levels of phenol compounds were decreased (p<0.05) by 68, 48, 26, and 9 as storage time increased as 0, 2, 4, 6wk, respectively. Phenol concentration was increased whereas p-cresol level was decreased as storage time increased showing ratios of phenol and p-cresol were 6:94, 34:66, 51:49, and 67:33 at 0, 2, 4, and 6wk, respectively. Concentration of indole compounds was not different for 2wk but increased (p<0.05) after 4wk. The ratios of indole and skatole were 71:29, 42:58, 28:72, and 36:64 at 0, 2, 4, and 6wk, respectively. Skatole concentration was increased as storage time increased. Therefore, our current results indicate that levels of volatile fatty acid and phenol compounds were deceased but indole compounds were increased as manure storage time was increased.

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.86-91
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• 2016

Oxidative stress caused by reactive oxygen species is ascribed to many neurodegenerative diseases like Alzheimer's disease. Phenolic antioxidants can reduce the oxidative stress. In this study, ripe fruits of Opuntia ficus-indica var. saboten (OFS) were extracted using 80% (v/v) aqueous ethanol. Total phenolic and flavonoid contents of the OFS fruits (100 g) were 409.9 mg gallic acid equivalents and 72.2 mg catechin equivalents, respectively. The OFS fruits had antioxidant capacity at 381.2, 298.2, and 3,219.9 mg vitamin C equivalents/100 g in ABTS, DPPH, and ORAC assays, respectively. The OFS fruits showed protective effects on PC-12 cells against oxidative stress in a dose-dependent manner, partly due to decrease of intracellular oxidative stress. Furthermore, the OFS fruits inhibited both acetylcholinesterase and butyrylcholinesterase. Consequently, these results suggest that the OFS fruits might be served as a source of functional materials to reduce oxidative stress in neuronal cells and to inhibit cholinesterases.