• Journal of Animal Reproduction and Biotechnology
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• v.37 no.3
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• pp.202-208
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• 2022

This study investigated the influence of sodium bicarbonate (NaHCO₃) and progesterone on acrosome reaction and proportion of polyunsaturated fatty acid (PUFA) composition boar sperm. The sperm were diluted with semen extender and incubated with NaHCO₃ and progesterone at 38℃, 5% CO₂ for 6 h. Plasma membrane integrity and acrosome reaction were analyzed using SYBR14/propidium iodide (PI) and FITC-PNA/PI doubling staining method, and proportion of PUFA was analyzed using gas chromatography. In results, Plasma membrane integrity was significantly decreased in 50 mM NaHCO₃ group and acrosome reaction was significantly increased by over the 100 mM NaHCO₃ group compared to control group (p < 0.05). In addition, progesterone significantly increased decreased plasma membrane integrity at 100 mM progesterone and acrosome reaction at over the 5.0 µM progesterone (p < 0.05), but there was no difference among the 5.0 to 100 µM groups. PUFAs were significantly decreased in 100 mM NaHCO₃ and 50 µM progesterone treatments compared to control group. In summary NaHCO₃ and progesterone induce acrosome reaction and reduce PUFA composition in boar sperm, therefore, the results maybe help to understand basically knowledge for the acrosome reaction and PUFA composition in boar sperm.

• Journal of the Korean Applied Science and Technology
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• v.38 no.5
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• pp.1282-1291
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• 2021

The aim of this study was to investigate the hematology and serum chemistry values on Sprague-Dawley rats, used sodium fluoride (NaF) for 5 weeks. Seven-week-old male rats were divided into nine groups and fed experimental diets with oral NaF treatment, basal diet group (BG), basal diet plus oral NaF groups (NF3~NF50). Concentrations of non-esterified fatty acid (NEFA), blood urea nitrogen (BUN), creatinine, uric acid in serum were significantly higher in the oral NaF groups than BG (𝛼=0.05). Total calcium (T-Ca) and phosphorus (Pi) concentrations was observed to be significant difference, it was decreased with an increase in the NaF levels (𝛼=0.05). Accordingly, sodium fluoride exposures and dose-response relationship, which was found on the bad influences in hematology. Such association is considered that the long-term fluoride exposure caused nephrotoxicity.

• Bulletin of the Korean Chemical Society
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• v.15 no.11
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• pp.967-971
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• 1994

The stability constants, log K of the 1 : 1 complexation for IA ions, $Ag^+$, and $Tl^+$ with a series of podands having different aromatic end-groups (I-IV) have been determined conductometrically in methanol at 25.0 $^{\circ}$C. Exceptionally the equivalent conductivity, ${\lambda}_{eq}\;of\;Li^+\;and\;Na^+$ were increased by the addition of I, because the complexed ions are less mobile than solvated ions. The order of log K values for I was $Ag^+{\gg}Tl^+>K^+>Na^+>Rb^+>Cs6+>Li^+$. The log K sequence of the podands for the certain cations was I>II>III${\geq}$IV. And every podands except IV showed the maximum selectivity for $Ag^+$ among the cations. These results were discussed in terms of the aromatic end-group effects, such as hetero-donor atoms or conformational changes by ${\pi}-{\pi}$ stacking interactions. The detailed conformations of ${\pi}-{\pi}$ stacking were also discussed by the observations of upfield shifts of some aromatic protons upon complexation from $^1H$ NMR spectra.

• Progress in Superconductivity
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• v.10 no.2
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• pp.123-127
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• 2009

NaFeAs recently observed superconductivity with the maximum $T_c{\approx}25$ K is investigated using first principles approach. We will address briefly the electronic structure and contrast other superconducting pnictides. This system shows strong two-dimensionality and reduction of flatness in the Fermi surfaces undermines tendencies of magnetic or charge instabilities. As observed in other superconducting pnictides, $Q_M=(\pi,\pi,0)$ antiferromagnetic ordering, which has not been observed clearly yet in this compound, is energetically favored. However, contrast to other superconducting pnictides, the density of states in this ordering shows considerable electron-hole asymmetry, implying efficiency of hole-doping than electron-doping to enhance $T_c$.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.525-528
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• 1999

Mutagenic activities of extracts from the filtrate of the cultured broth (PI-I), mycelia (pI-II), and the fruiting bodies (PI-III) of Phellinus igniarius were examined by Ames/Salmonella tests. No mutagenic activity was found in Salmonella typhimurium strains TA98 and TA100, either with or without S9 activation. In contrast, PI-I, PI-II, and PI-III showed inhibitory effects on the mutagenic activities by the directly-acting mutagens, 4-nitro-ο-phenylenediamine(NPD) and sodium azide ($NaN_3$), and also by the indirectly-acting mutagens, 2-aminofluorene (2-AF) and benzo[a]pyrene (B[a]P). These results suggest that P. igniarius possesses some antimutagenic activity and may contain some chemopreventive agents.

• Journal of Acupuncture Research
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• v.20 no.6
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• pp.128-139
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• 2003

Juglans sinensis Dode has been reported to have antioxidant activity. However, the effect of Juglans sinensis Dode aquacupuncture(JS) on reactive oxygen species(ROS)-induced alterations in membrane transport function in renal tubular cells. This study was performed to evaluate the effect of JS on the organic hydroperoxide t-butylhydroperoxide(tBHP)-induced inhibition of $Na^+$-dependent phosphate($Na^+$-Pi) uptake in opossum kidney (OK) cells, an established renal proximal epithelial cell line. tBHP inhibited $Na^+$-Pi uptake in a time-dependent manner. The inhibitory effect of tBHP was prevented by JS over concentration range of 0.05-1mg/100ml in a dose-dependent manner. Kinetic studies showed that tBHP caused an decrease in Vmax for $Na^+$-Pi uptake without any a significant change in Km. $Na^+$-dependent phosphonoformic acid binding, a irreversible inhibitor of renal $Na^+$-Pi uptake, was decreased by tBHP treatment. The reduction in Vmax and phosphonoformic acid binding by tBHP was prevented by JS. tBHP induced lipid peroxidation and its effect was completely inhibited by JS and antioxidant N,N'-diphenyl-p-phenylenediamine. These data suggest that the oxidant inhibits phosphate uptake by a reduction in the number of active carrier across the membrane. JS may prevent oxidant-induced inhibition of membrane transport function by a mechanism similar to antioxidants in renal epithelial cells. Although the precise constituents remain to be explored, JS may be employed as a useful candidate herb for drug development to prevent and treat oxidant-mediated renal failure.

• The Science & Technology
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• no.5 s.432
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• pp.55-57
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• 2005

• Korean Chemical Engineering Research
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• v.52 no.3
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• pp.328-334
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• 2014

This study examined the effect of concentration polarization on permeate flux in forward osmosis (FO) membrane process for saline and sucrose solution. The reduction in permeate flux during the FO membrane process is largely due to the formation of concentration polarization on membrane surfaces. The flux reduction due to internal concentration polarization formed on the porous support layer was larger than that due to the external concentration polarization on the active membrane surface. Water permeate flux through the FO membrane increased nonlinearly with the increase in osmotic pressure. The water permeability coefficient was $1.8081{\times}10^{-7}m/s{\cdot}atm$ for draw solution on active layer (DS-AL) mode and $1.0957{\times}10^{-7}m/s{\cdot}atm$ for draw solution on support layer (DS-SL) mode in NaCl solution system. The corresponding membrane resistance was $5.5306{\times}10^6$ and $9.1266{\times}10^6s{\cdot}atm/m$, respectively. With respect to the sucrose solution, the permeate flux for DS-AL mode was 1.33~1.90 times higher than that for DS-SL mode. The corresponding variation in the permeation flux (J) due to osmotic pressure (${\pi}$) would be expressed as $J=-0.0177+0.4506{\pi}-0.0032{\pi}^2$ for the forward and $J=0.0948+0.3292{\pi}-0.0037{\pi}^2$ for the latter.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.1
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• pp.7-13
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• 1990

Nutritional status of cattle in Java in Indonesia was investigated by evaluating P, Ca, Mg, K and Na concentrations in forage and plasma inorganic phosphorus (Pi), Ca and Mg, hematocrit and hemoglobin concentrations of cattle during both rainy and dry seasons in 1988. Investigations were conducted at Jonggol (West Java), Malang (East Java) and Majokerto (East Java). Low P content in forage averaging 0.11% on a dry matter basis, high Ca:P ratio in forage in the rainy season (7.7:1) and low plasma Pi concentrations averaging 4.71 mg/dl were observed in Jonggol. In all three places, Ca content in forages was higher than the requirement according to the NRC standard and plasma Ca concentrations were above the critical level. During the rainy season forty percent of cattle in Malang and Mahokerto showed plasma Mg concentrations below 2mg/dl in spite of normal Mg and K content in forage. Forage samples from each place showed a wide variety in Na content, and Na content of two thirds of forage samples was lower than the requirement according to the NRC standard. Hematocrit and hemoglobin concentrations in Malang and Majokerto showed higher values than the normal range.

• Applied Biological Chemistry
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• v.37 no.3
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• pp.189-193
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• 1994

The hydrolysis of fungicidal N-[1-(benzotriazol-1-yl)benzyl]aniline (BBA) molecule in the presence of cationic cetyltrimethylammonium bromide (CTABr) and anionic sodium laurylsulfate (NaLs) micellar solutions has been studied kinetically. The Micellar catalysis effect shows that the rate is slightly accelerated by the addition of the anionic NaLs at high pH and the binding constant (Ks) is $1.45{\times}10^4M^{-1}$. This result presumably is due to the electrostatic stabilization by the anionic micelle of the developing carbocation in the transition state rather than the hydrophobic character (${\pi}$: 4.93) of (BBA).