• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.8
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• pp.1183-1189
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• 2013

We investigated the effects of oyster shell extract (OSE) on papain-induced osteoarthritis in C57BL/6J mice. Osteoarthritis was induced in mice by a papain injection into the knee joint. The mice were divided into a total of five groups (n=8). The normal group was untreated, whereas the papain group (negative control) was induced with osteoarthritis and treated with water daily. The papain+DS group (positive control) was treated with diclofenac sodium. Papain+OSE groups were treated with OSE concentrations of 100 and 200 mg/kg/bw for 20 days. Proteoglycan content in articular cartilage was analyzed through safranine-O fast green staining and H&E staining. The histopathological changes in cartilage were measured by the Rudolphi score approach. The contents of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin (IL)-$1{\beta}$, and IL-6 in plasma were analyzed by the ELISA method. After experiments, body weights of the treated groups were not significantly different compared with the normal group. Cartilage loss and joint instability significantly improved in a dose-dependent manner in the OSE-treated group compared with the papain group (P<0.05). Proteoglycan content was significantly higher in the OSE-treated group than the papain group (P<0.05). Osteoarthritis scores of the OSE-treated group were significantly decreased compared with the papain group (P<0.05). TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 content in the plasma of the papain+OSE treated groups significantly decreased in a dose-dependent manner compared with the papain group (P<0.05). These results suggest that OSE treatment might have anti-arthritic effects on papain-induced osteoarthritis in C57BL/6J mice.

• Advances in pediatric surgery
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• v.19 no.1
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• pp.1-13
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• 2013

The Korean Association of Pediatric Surgeons (KAPS) performed the second nationwide survey on biliary atresia in 2011. It was a follow-up study to the first survey, which was performed in 2001 for the retrospective analysis of biliary atresia between 1980 and 2000. In the second survey, the authors reviewed and analyzed the clinical data of patients who were treated for biliary atresia by the members of KAPS from 2001 to 2010. A total of 459 patients were registered. Among them, 435 patients primarily underwent the Kasai operation. The mean age of patients who underwent the Kasai operation was $66.2{\pm}28.7$ days, and 89.7% of those patients had type III biliary atresia. Only five patients (1.4%) had complications related to the Kasai operation. After the Kasai operation, 269 (61.8%) of the patients were re-admitted because of cholangitis (79.9%) and varices (20.4%). One hundred and fifty-nine (36.6%) of the patients who underwent the Kasai operation subsequently underwent liver transplantation. The most common cause of subsequent liver transplantation was persistent hyperbilirubinemia. The mean interval between the Kasai operation and liver transplantation was $1.1{\pm}1.3$ years. Overall the 10-year survival rate after the Kasai operation was 92.9% and the 10-year native liver survival rate was 59.8%. We had 23 patients for primary liver transplantation without the Kasai operation. The mean age patients who underwent primary liver transplantation was $8.6{\pm}2.9$ months. In summary, among the 458 Kasai-operation and liver-transplantation patients, 373 lived, 31 died, and 54 were unavailable for follow up. One-third of the patient who survived have had complications correlated with biliary atresia. In comparison with the first survey, this study showed a higher survival rate and a greater number of liver transplantation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.8
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• pp.1055-1061
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• 2007

Caesalpinia sappan L. is an indeciduous tree distributed in China, India, Miyanmar and Vietnam. Its heartwood has long been used in oriental folk medicines to treat diseases. In this study, antioxidative activities of Caesalpinia sappan L. and the effect of gamma irradiation on its chemical and biological properties were investigated. The ethyl acetate fraction (EtOAc fr.) of Caesalpinia sappan L. was irradiated with 100 kGy of gamma ray. The dark red color of EtOAc fr. was significantly (p<0.05) removed by irradiation (Hunter L and b values increased and a value decreased). The total phenolic content of EtOAc fr. was 865 mg/g and it was increased to 1195 mg/g by gamma irradiation. DPPH radical and superoxide anion radical scavenging activities and lipid peroxidation inhibitory activity of EtOAc fr. were very high and its activities were also increased by gamma irradiation. EtOAc fr. also inhibited the irradiation-induced DNA damage of lymphocyte as determined by comet assay. In conclusion, EtOAc fr. of Caesalpinia sappan L. extract showed high antioxidative activities in vitro. Furthermore, gamma irradiation on EtOAc fr. ameliorated the color and antioxidative properties. Therefore, it can be suggested that Caesalpinia sappan L. may be a good material for antioxidant function and gamma irradiation may be applied for the improvement of chemical and biological properties of Caesalpinia sappan L.

• Korean Journal of Environmental Agriculture
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• v.6 no.1
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• pp.1-6
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• 1987

A pot experiment was conducted to find out the effect of water management on the growth and uptake of arsenic and inorganic nutrients of rice plant at As added soil. The arsenic were added to soil at the levels of As 0, 10, 50, 100 and 150 ppm, respectively. Water management was done with two ways: intermittent irrigation from ten days after transplanting, and continuous submersion until harvest. Higher soil As levels increased As content in plant but reduced growth rate. Aresenic content in plant was considerably reduced with intermittent irrigation compared to continuous submersion. Rice growth showed also same trend. With increasing As levels in soil, N content in plant was increased but P, K, Ca, Mg, $SiO_2$, Fe and Mn content in plant were tend to be decreased. These inorganic nutrients in plant were also much absorbed in continuous submersion compared to intermittent irrigation. Soil pH was slightly increased with increasing As levels in soil while soil Eh has no relationship with soil As levels. On the other hand, soil pH was higher in the treatment of continuous submersion than that of intermittent irrigation but soil Eh showed reverse trend. With increasing As levels in soil, water soluble-As and Ca-As fractions in soil tend to be increased with continuous submersion, but these fractions has no tendency with intermittant irrigation.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.165-172
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• 2004

This study was conducted to investigate changes in physico-chemical characteristics of Hanwoo carcass according to different castration and slaughtering ages. Total 75 Hanwoo calves(average 4 months old) were randomly assigned to Control(bulIs). Treatment I. Il , III. or IV(castrated on 4. 9. 12. and 16 months of age, respectively) and slaughtered at 7. 9. 12, 16, and 28 months of age. Moisture contents in M. Longissimus dorsi of both Hanwoo bulls and steers were decreased as the slaughtering age increased. Moisture contents in all steer groups were significantly(P <0.05) lower(average 68.63%) than that in bulls(72.18%) at 28 months of age. Crude fat contents tended to increase as the slaughtering age increased. Crude fat contents in Treatment III(castrated at 12 months of age) at 28 months of age were 10.24% which was the highest among all steer groups. Crude protein contents in M. Longissimus dorsi of Hanwoo bulls were around 23% regardless of slaughtering age. Water holding capacity(WHC) of both bulls and steers tended to increase as the animals aged. Steers showed rapid increase in WHC after 16 months of age and showed higher(78.39%)) WHC than bulls(70.00%) at 28 months of age. Palmitic acid(CI8: 0) contents in Hanwoo bulls were increased as the slaughtering age increased(20.92 \longrightarrow 24.22%) whereas stearic acid(CI8: 0) contents were decreased(I8.l5 \longrightarrow11.32%). Oleic acid(CI8: I). a major unsaturated fatty acid. significantly(P <0.05) increased(25.59 \longrightarrow 37.90%) when slaughtering age of Hanwoo increased. Changes in fatty acid composition in Hanwoo steers were similar to those in bulls except oleic acid contents were higher and stearic acid contents were lower than bulls at 28 months of age.

• Proceedings of the KOR-BRONCHOESO Conference
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• 1972.03a
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• pp.6-7
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• 1972

The air pollutants can be classified into the irritant gas and the asphixation gas, and the irritant gas is closely related to the otorhinolaryngological diseases. The common irritant gases are nitrogen oxides, sulfur oxides, hydrogen carbon compounds, and the potent and irritating PAN (peroxy acyl nitrate) which is secondarily liberated from photosynthesis. Those gases adhers to the mucous membrane to result in ulceration and secondary infection due to their potent oxidizing power. 1. Sulfur dioxide gas Sulfur dioxide gas has the typical characteristics of the air pollutants. Because of its high solubility it gets easily absorbed in the respiratory tract, when the symptoms and signs by irritation become manifested initially and later the resistance in the respiratory tract brings central about pulmonary edema and respiratory paralysis of origin. Chronic exposure to the gas leads to rhinitis, pharyngitis, laryngitis, and olfactory or gustatory disturbances. 2. Carbon monoxide Toxicity of carbon monoxide is due to its deprivation of the oxygen carrying capacity of the hemoglobin. The degree of the carbon monoxide intoxication varies according to its concentration and the duration of inhalation. It starts with headache, vertigo, nausea, vomiting and tinnitus, which can progress to respiratory difficulty, muscular laxity, syncope, and coma leading to death. 3. Nitrogen dioxide Nitrogen dioxide causes respiratory disturbances by formation of methemoglobin. In acute poisoning, it can cause pulmonary congestion, pulmonary edema, bronchitis, and pneumonia due to its strong irritation on the eyes and the nose. In chronic poisoning, it causes chronic pulmonary fibrosis and pulmonary edema. 4. Ozone It has offending irritating odor, and causes dryness of na sopharyngolaryngeal mucosa, headache and depressed pulmonary function which may eventually lead to pulmonary congestion or edema. 5. Smog The most outstanding incident of the smog occurred in London from December 5 through 8, 1952, because of which the mortality of the respiratory diseases increased fourfold. The smog was thought to be due to the smoke produced by incomplete combustion and its byproduct the sulfur oxides, and the dust was thought to play the secondary role. In new sense, hazardous is the photochemical smog which is produced by combination of light energy and the hydrocarbons and oxidant in the air. The Yonsei University Institute for Environmental :pollution Research launched a project to determine the relationship between the pollution and the medical, ophthalmological and rhinopharyngological disorders. The students (469) of the "S" Technical School in the most heavily polluted area in Pusan (Uham Dong district) were compared with those (345) of "K" High School in the less polluted area. The investigated group had those with subjective symptoms twice as much as the control group, 22.6% (106) in investigated group and 11.3% (39) in the control group. Among those symptomatic students of the investigated group. There were 29 with respiratory symptoms (29%), 22 with eye symptoms (21%), 50 with stuffy nose and rhinorrhea (47%), and 5 with sore thorat (5%), which revealed that more than half the students (52%) had subjective symptoms of the rhinopharyngological aspects. Physical examination revealed that the investigated group had more number of students with signs than those of the control group by 10%, 180 (38.4%) versus 99 (28.8%). Among the preceding 180 students of the investigated group, there were 8 with eye diseases (44%), 1 with respiratory disease (0.6%), 97 with rhinitis (54%), and 74 with pharyngotonsillitis (41%) which means that 95% of them had rharygoical diseases. The preceding data revealed that the otolaryngological diseases are conspicuously outnumbered in the heavily polluted area, and that there must be very close relationship between the air pollution and the otolaryngological diseases, and the anti-pollution measure is urgently needed.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.147-155
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• 1977

In this experiment, we expected yield increase depending on the control of ineffective tiller, heightening of effective tillering ratio and continuous supply of nitrogen until later growth stage of rice plant by deep layer split application. Treats were applied at Tongil and Jinheung variety, clayey loam and sandy loam soil, and drained and non-drained condition. Nitrogenous fertilizer application wab adopted as liquefied(50%) and lumped (50% and 80%) fertilizer at 12cm depth of soil before 35 days of rice heading time against the standard soil surface application. The results are summarized as follaw. 1. a. Jinheung showed great variant width of tiller numbers per rice plant growth stage, and low effective tillering ratio at soil surface dressing. But in the case of deep layer split application, the number of tiller increased normally, and effective tillering ratio was high. b. At Tonsil, the width of increase and decrease range of effective tiller number between soil surface dressing and deep layer split application was not so high as Jinheung. Deep layer split application of 80% lumped fertilizer showed maximum effective tillering ratio ($83%{\sim}93%$). C. In the case of Jinheung, it was supposed that deep layer split application of 80% lumped fertilizer was excessive nitrogen quantity. d. Effective tillering ratio was higher than Tonsil at Jinheung. 2. The number of grains per hill was increased by the deep layer split application, but the ripening ratio was decreased inversely with the increase of total grain number. 3. Length of top leaves was elongated at Jinheung by deep layer split application. It showed significant correlation between top leaves length and grain yield. 4. Deep layer split application inclosed N content of harvested straw. Yield and N content of straw showed possitive correlation. 5. The ratio of unhulled grain yield per straw weight was increased by deep layer splication. This ratio was higher at Jinheung than Tonsil. 6. Grain yield was appeared in order of 80% lumped fertilizer>50% lumped fertilizer>50% liquefied fertilizer>surface dressing by the deep layer split application. The yield increasing factors were the increasing of effective tillering ratio, number of panicles per hill and number of ripening grains per hill. 7. Grain yield was increased at Tongil in sandy loam soil and at Jinheung in clayey loam soil by deep layer split application. 8. The grain yield was increased at drained conditions of clayey loam soil and non-drained conditions of sandy loam soil. But in the case of 80% lumped fertilizer of deep layer split application at the sandy loam soil, the yield was not increased at non-drained conditions. 9. The effect of yield increase by deep layer split application comparing with the surface dressing was higher at Tonsil than ginheung, in spite of low ripening ratio of Tonsil caused by low temperature at heading and harvesting time.

• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.465-478
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• 1995

The objective of this study was to investigate the impacts of large-scale timber harvesting on the environment of a mature hardwood forest. To achieve the objective, the effects of harvesting on forest environmental factors were analyzed quantitatively using the field data measured in the study sites of Seoul National University Research Forests [(Mt.) Paekunsan] for two years(1993-1994) following timber harvesting. The field data include information on vegetation, soil mesofauna, physicochemical characteristics of soil, surface water runoff, water quality in the stream, and hillslope erosion. For comparison, field data for each environmental factor were collected in forest areas disturbed by logging and undisturbed, separately. The results of this study were as follows : The diversity of vegetational species increased in the harvested sites. However, the similarity index value of species between harvested and non-harvested sites was close to each other. Soil bulk density and soil hardness were increased after timber harvesting, respectively. The level of organic matter, total-N, avail $P_2O_5$, CEC($K^+$, $Na^+$, $Ca^{{+}{+}}$, $Mg^{{+}{+}}$) in the harvested area were found decreased. While the population of Colembola spp., and Acari spp. among soil mesofauna in harvested sites increased by two to seven times compared to those of non-harvested sites during the first year, the rates of increment decreased in the second year. However, those members of soil mesofauna in harvested sites were still higher than those of non-harvested sites in the second year. The results of statistical analysis using the stepwise regression method indicated that the diversity of soil mesofauna were significantly affected by soil moisture, soil bulk density, $Mg^{{+}{+}}$, CEC, and soil temperature at soil depth of 5(0~10)cm in the order of importance. The amount of surface water runoff on harvested sites was larger than that of non-harvested sites by 28% in the first year and 24.5% in the second year after timber harvesting. The level of BOD, COD, and pH in the stream water on the harvested sites reached at the level of the domestic use for drinking in the first and second year after timber harvesting. Such heavy metals as Cd, Pb, Cu, and organic P were not found. Moreover, the level of eight factors of domestic use for drinking water designated by the Ministry of Health and Welfare of Korea were within the level of the first class in the quality of drinking water standard. The study also showed that the amount of hillslope erosion in harvested sites was 4.77 ton/ha/yr in the first year after timber harvesting. In the second year, the amount decreased rapidly to 1.0 ton/ha/yr. The impact of logging on hillslope erosion in the harvested sites was larger than that in non-harvested sites by seven times in the first year and two times in the second year. The above results indicate that the large-scale timber harvesting cause significant changes in the environmental factors. However, the results are based on only two-year field observation. We should take more field observation and analyses to increase understandings on the impacts of timber harvesting on environmental changes. With the understandings, we might be able to improve the technology of timber harvesting operations to reduce the environmental impacts of large-scale timber harvesting.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.6
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• pp.570-581
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• 2013

To improve productivity of aquaculture animals with management of culturing grounds, survey of mariculture management area in Ongjin-gun about water quality and sedimentary environment had been conducted on June, August and November in 2011. Water temperature in surface and bottom waters ranged from 9.49 to $24.14^{\circ}C$. Salinity and dissolved oxygen concentrations were in the range of 23.19~31.49 and 5.48~9.36 mg/L, respectively, depending on the variation of water temperature. The average concentration of COD was 1.57 mg/L and the concentrations of DIN and DIP showed entirely low level. As the result of grain size analysis, sand(56.66 %) and silt(34.60 %) were predominated. The Mz of sediment showed a variation of 2.59 to $6.62{\O}$ and sorting appeared to be poorly sorted. The concentrations of COD and IL in surface sediment ranged from 1.00 to $11.03mg/g{\cdot}dry$ and 0.72 to 5.29 %, respectively, which showed relatively good positive correlations. On the environmental assessment of trace metals in surface sediment, geoaccumulation index ($I_{geo}$) class indicated that sediments were not contaminated by most of metallic elements except Cr and As. Our result implies that this study area showed good water quality and sediments were not polluted by organic matters and metallic elements.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.323-330
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• 2013

This study was conducted to know the effect of major physical-chemical characteristics of soil to be in the area of indigenous and naturalized plant in the sand dune of the western coastal area of Korea. The study was performed on the sand dunes distributed across Taean-gun and Buan-gun and the study period was from April to October in 2010. Sixty nine of $5m{\times}5m$ study plots were installed and the distribution of plants were investigated. We measured the soil characteristics including soil pH, organic matter, total nitrogen, available $P_2O_5$, soil cation exchange capacity, exchangeable cation, EC and NaCl. As a result, soil texture was classified as sand, in case of average pH, NaCl, and EC of soil to be in the area of indigenous herbaceous plant were 7.77, 0.03% and $0.52ds.m^{-1}$, indigenous woody plant of the mean pH, NaCl and EC were 7.31, 0.01%, $0.23ds.m^{-1}$. In case of naturalized herbaceous plant of the mean soil pH, NaCl and EC were 7.12, 0.01%, $0.29ds.m^{-1}$, naturalized woody plant of the mean soil pH, NaCl and EC were 7.34, 0.01%, $0.20ds.m^{-1}$ respectively. On average, naturalize plants showed in low salinity concentration than indigenous plants.