Park, Soon Dal;Kim, Jung Suk;Joe, Kih Soo;Kim, Jong Gu;Kim, Won Ho
Analytical Science and Technology
/
v.17
no.3
/
pp.251-262
/
2004
It is for the purpose of investigating the tritium distribution in the leachates, the raw and treated leachates and the condensates of the methane gas, which have occurred from domestic solid waste landfills. Also it aims to measure the tritium distribution level on the colloid size of the leachates, the raw and treated leachates. It was found that the major inorganic contaminants of the leachates were Na, K, Ca, Mg, $NH{_4}^+$-N and $Cl^-$. The mean tritium level of the raw leachates of the investigated 13 landfill sites for 6 months was 17 ~ 1196 TU. It corresponded to a several scores or hundreds of magnitude higher value than that of the normal environmental sample level except for two landfill sites. Also such a high concentration of the tritium was found in the treated leachates and methane gas condensates as well. Nevertheless it is important to emphasize that the tritium level which was found in this research is about 100 times lower than the tritium limit for the drinking water quality. And most of the tritium existed in the dissolved colloid of the leachate of which the colloid size is below $0.45{\mu}m$. Also, according to the tritium analysis results of the leachates after filtration with $0.45{\mu}m$ membrane filter for some landfills, it is likely that some tritium of the leachate would be distributed in a colloid size over $0.45{\mu}m$. In general the relationship between the tritium and other contaminants in the raw leachate was low, but it was relatively high between the tritium and TOC. However, the tritium content in the leachate had no meaningful relationship with the scale, hydrological characteristics and age of the landfill.
Seok, Sang Il;Ahn, Bok Yeop;Suh, Tae Soo;Rhee, Dong Seok
Journal of Korean Society of Environmental Engineers
/
v.22
no.4
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pp.765-773
/
2000
The degradation of humic acid using $TiO_2$ coatings was studied, $TiO_2$ coatings were prepared by dip-coating method. Sol solutions for coating were prepared by mixing the gel, which can be produced by the reaction of $TiOCl_2$ and $NH_4OH$ solution, and hydrogen peroxide solution, and hydrolysis of titanium tetraisopropoxide (TTIP). It was shown from XRD that coatings from sol aged at $100^{\circ}C$ for 18h with titanium peroxo solution were crystallized to anatase in the range of temperatures of $25^{\circ}C$ to $500^{\circ}C$. In contrast, those coated from TTIP were crystallized to anatase at temperature above $400^{\circ}C$. So the sols originated from $TiCl_4$ can be applied for not only on the heat-resistance substrates but on the plastic substrates. Thickness and the quality of the films were dependent on the withdrawing speed, the concentration of sol, and the number of coating. The films showed various interference colors depending on the thickness of them. In the case that the films coated 2 times at withdrawing speed of 2.5cm per minute by 0.2M sol, the films had a transparent light blue color with thickness of around 50nm. It was known from the result of photo-degradation by $TiO_2$ coatings using humic acid that the removal efficiency of $COD_{cr}$ was over 85% after illumination of $UV/H_2O_2$ for 40min. and that of UV/VIS absorbable materials was over 95%.
Feather, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common professes. Four strains, SMMJ-2, FL-3, NO-4 and RM-12 were isolated from soil for production of extracellular keratinolytic protease. They were identified as Bacillus sp. based on their morphological and physiological characteristics. They shown high protease activity on 5.0% skim milk agar medium and produced a substrate like mucoid on keratin agar medium. Bacillus sp. SMMJ-2 had a faster production time for producing keratinolytic protease than other strains. This strain did not completely degrade whole chicken feather for five days in basal medium but completely degraded whole chicken feather when supplied with nitrogen source for 40hours in keratinolytic producing medium ($0.7%\;K_{2}HPO_{4},\;0.2%\;KH_{2}PO_{4},\;0.1%$ fructose, 1.2% whole chicken feather, $0.01%\;Na_{2}CO_3$, pH 7.0). When supplied with chicken feather as nitrogen source, keratinolytic protease activity was 89 units/ml/min. When soybean meal was used as nitrogen source, the keratinolytic protease production reached a maximum of 106 units/ml/min after 48 hours under $30^{\circ}C$, 180 agitation. To isolate the keratinolytic protease, the culture filtrate was precipitated with $(NH_4)_{2}SO_4$ and acetone. The recovery rate of keratinolytic protease was about 96% after treatment with 50% acetone. The enzyme was stable in the range of $30{\sim}50^{\circ}C$ and pH $6.0{\sim}12.0$.
Kim, Jack-C.;Kim, Si-Hwan;Kim, Ji-A;Choi, Soon-Kyu;Park, Won-Woo
Archives of Pharmacal Research
/
v.21
no.4
/
pp.458-464
/
1998
Search for a new $\alpha$-methylene-$\gamma$-butyrolactone-bearing 6-substituted purine as a potental antitumor agent has led to synthesize seven, hitherto unreported, $5^1$-Methyl-$5^1$-[(6-substituted-9H-purin-9-yl)methyl]-$2^1$-oxo-$3^1$- methylenetetrahydrofurans (H, Cl, l, $CH_3$, $NH_2$, SH, >C=O) (6a-g). These include $5^1$-Methyl-$5^1$-[(9H-purin-9-yl)methyll-$2^1$-oxo-$3^1$ -methylenetetrahydrofurans (6a), $5^1$-Methyl-$5^1$-[(6-chloro-9H-purin-9-yl)methyl]-$2^1$-oxo-$3^1$-methylenetetrahydr ofurans (6b), $5^1$-Methyl-$5^1$-[(6-chloro-9H-purin-9-yl) methyl]-$2^1$-oxo-$3^1$-methylenetetrahydrofurans (6c), $5^1$-Methyl-$5^1$-[(6-methyl-9H-purin-9-yl) methyl]-$2^1$-oxo-$3^1$-methylenetetrahydrofurans (6d), $5^1$-Methyl-$5^1$-[(9H-adenin-9-yl)methyll-$2^1$-oxo-$3^1$-methylenetetrahydrofurans (6e), $5^1$-Methyl-$5^1$-[(6-mercapto-9H-purin-9-yl) methyl]-$2^1$-oxo-$3^1$-methylenetetrahydrofurans (6f) and $5^1$-Methyl-$5^1$-[(9H-hypoxanthin-9-yl)methyll-$2^1$-oxo-$3^1$-methylenetetrahydrof urans (6g) which were made by the Reformatsky-type reaction of ethyl $\alpha$-(bromomethyl) acrylate with the corresponding (6-substituted-9H-purin-9-yl)-2-propanone intermediates (5a-g). These ketone intermediates 5a-g, 1-(9H-purin-9-yl)-2-propanone (5a), 1-(6-chloro-9H-purin-9-yl)-2-propanone (5b), 1-(6-iodo-9H-purin-9-yi)-2-propanone (5c), 1-(6-methyl-9H-purin-9-yl)-2-propanone (5d), 1-(9H-adenin-9-yl)-2-propanone (Se), 1-(6-mercapto-9H-purin-9-yl)-2-propanone (5f), and 1-(9H-hypoxanthin-9-yl)-2-propanone (5g) were directly obtained by the alkylation of the 6-substituted purine bases with the chloroacetone in the presence of $K_2$$CO_3$ (or NaH) under DMF (or DMSO). The preliminary in vitro cytotoxcity assay for the synthetic .alpha.-methylene-y-butyro-lactone compounds (6a-g) were determined against three cell lines (PM-3A, P-388, and K-562) and showed the moderate antitumor activity ($IC_50$ ranged from 1.4 to 4.3 $\mu\textrm{g}$/ml) with the compound $5^1$-methyl-$5^1$ -[(9H-hypoxanthin-9-yl)methyl]-$2^1$-oxo-$3^1$-methylenetetrahydrofuran (6g) showing the least antitumor activity.
Journal of Korean Society of Environmental Engineers
/
v.27
no.5
/
pp.499-506
/
2005
This study was performed to investigate the effects of influent C/N ratio on the removal of organic and nitrogenous compounds by two nonwoven fabric filter bioreactors. The reactors were alternately aerated at an aeration/nonaeration period ratio of 60 min/60 min, and fed with wastewater only during nonaeration period. The influent C/N ratio (COD/TKN) was gradually reduced from 10 to 2. The influent was prepared by diluting the leachate from a foodwaste treatment facility in I city so that the COD concentration could be about 2,500 mg/L. The C/N ratio of the wastewater was adjusted by adding ammonium chloride. The results of the experiment showed that the COD and BOD concentration of the effluent was $40{\sim}54\;mg/L$ and $1{\sim}4\;mg/L$, respectively at the C/N ratios of $10{\sim}3$, and the effluent SS concentration was always below 2.0 mg/L. The T-N removal efficiencies were 96% or higher at C/N ratios of $10{\sim}5$, but decreased to 83% and 81%, respectively at the C/N ratios of 3 and 2.8. At the C/N ratios of 2.6 and 2, the effluent quality deteriorated due to ammonia toxicity. The fraction of nitrifying microorganism in the reactors increased from 10% to 20% as the C/N ratio decreased from 5 to 2.6. Alkalinity consumed were $3.12{\sim}3.49\;g$ alkalinity/g T-N removed at the C/N ratios of $10{\sim}5$, which are lower than the theoretical value of 3.57. However, the ratio increased to 4.63 and 4.87 g alkalinity/g T-N removed, respectively at the C/N ratios of 3 and 2.8.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.7
no.2
/
pp.60-67
/
2002
To determine whether nitrogen (N) uptake by phytoplankton below the euphotic zone in the Yellow Sea is considerable, we measured the uptake rates of nitrate and ammonium using $^{15}N$-labeled stable isotope $K^{15}NO_{3}$ and $^{15}NH_{4}Cl$, in May and November 1997 at total 10 stations. Depth-integrated uptake rates of nitrate and ammonium over the euphotic zone during this study ranged from 1.8 to 15.3 mg N $m^{-2}$$d^{-1}$ and from 5.0 to 132.2 mg N $m^{-2}$$d^{-1}$, respectively, and ammonium uptake predominated at 9 of 10 stations (1.9-19.4 fold). Depth-integrated uptake rates of nitrate and ammonium over the whole water column ranged from 2.9 to 22.0 mg N $m^{-2}$$d^{-1}$ and from 15.7 to 175.5 mg N $m^{-2}$$d^{-1}$, respectively. The significant proportion of whole water column N uptake was attributed to uptake by phytoplankton below the euphotic zone, ranging from 13.0 to 86.2% for nitrate and from 13.8 to 67.8% for ammonium, indicating that phytoplankton N uptake below the euphotic zone is at times considerable in the study area. The results suggest that when phytoplankton below the euphotic zone in the Yellow Sea are again entrained into the euphotic zone by a certain physical forcing such as turbulent mixing and the vertical movement of thermocline, these episodic events may significantly affect the material fluxes within the euphotic zone. Furthermore, the results suggest that a portion of regenerated production estimated from $^{15}N$-ammonium uptake should be included in new production estimates, which otherwise could be underestimated in the Yellow Sea.
Kim Tae-Hyun;Kim Hyung-Joon;Park Joon-Sung;Kim Younhee;Lee Heung-Shick
Korean Journal of Microbiology
/
v.41
no.2
/
pp.99-104
/
2005
Corynebacterial clones which exert regulatory effects on the expression of the glyoxylate bypass genes were isolated using a reporter plasmid carrying the enteric lacZ fused to the aceB promoter of Corynebacterium glutamicum. Some clones carried common fragments as turned out by DNA mapping technique. Subcloning analysis followed by the measurement of $\beta-galactosidase$ activity in Escherichia coli identified the region responsible for the aceB-repressing activity. Sequence analysis of the DNA fragment identified two independent ORFs of ORF1 and ORF2. Among them, ORF2 was turned out to be responsible for the aceB-repressing activity. ORF1 encoded a 23,216 Da protein composed of 206 amino acids. Sequence similarity search indicated that the ORF may encode a ECF-type $\sigma$ factor and designated sigH. To identify the function of sigH, C. glutamicum sigH mutant was constructed by gene disruption technique and the sigH mutant showed growth retardation as compared to the wild type strain. In addition, the mutant strain showed sensitivity to oxidative-stress generating agent plumbagin. This result imply that sigH is probably involved in the stress response occurring during normal cell growth.
Purpose: Chitosan has been studied as a non-viral gene delivery vector, drug delivery carrier, metal chelator, food additive, and radiopharmaceutical, among other things. Recently, galactose-graft chitosan was studied as a non-viral gene and drug delivery vector to target hepatocytes. The aim of this study was to investigate the usefulness of nuclear imaging for in vivo evaluation of targeting the hepatocyte by galactose grafting. Methods and Materials: Galactosyl methylated chitosan (GMC) was produced by methylation to lactobionic acid coupled chitosan. Cytotoxicity of $^{99m}Tc$-GMC was determined by MTT assay. Rabbits were injected via their auricular vein with $^{99m}Tc$-GMC and $^{99m}Tc$-methylated chitosan (MC), the latter of which does not contain a galactose group, and images were acquired with a gamma camera equipped with a parallel hole collimator. The composition of the galactose group in galactosylated chitosan (GC), as well as the tri-, di-, or mono-methylation of GMC, was confirmed by NMR spectroscopy. Results: The results of MTT assay indicated that $^{99m}Tc$-GMC was non-toxic. $^{99m}Tc$-GMC specifically accumulated in the liver within 10 minutes of injection and maintained high hepatic uptake. In contrast, $^{99m}Tc$-MC showed faint liver uptake. $^{99m}Tc$-GMC scintigraphy of rabbits showed that the galactose ligand principally targeted the liver while the chitosan functionalities led to excretion through the urinary system. Conclusion: Bioconjugation with a specific ligand endows some degree of targetability to an administered molecule or drug, as in the case of galactose for hepatocyte in vivo, and evaluating said targetabililty is a clear example of the great benefit proffered by nuclear imaging.
Mineral carbonation is a technology in which carbonates are synthesized from minerals including serpentine and olivine, and industrial wastes such as slag and cement, of which all contain calcium or magnesium when reacted with carbon dioxide. This study aims to develop the mineral carbonation technology for commercialization, which can reduce environmental burden and process cost through the reduction of carbon dioxide using steel slag and the slag reuse after calcium extraction. Calcium extraction was conducted using NH4Cl solution for air-cooled slag and convert slag, and ${\geq}98%$ purity calcium carbonate was synthesized by reaction with calcium-extracted solution and carbon dioxide. And we conducted experimentally to minimize the quantity of by-product, the slag residue after calcium extraction, which has occupied large amount of weight ratio (about 80-90%) at the point of mineral carbonation process using slag. The slag residue was used to replace silica sand in the manufacture of cement panel, and physical properties including compressive strength and flexible strength of panel using the slag residue and normal cement panel, respectively, were analyzed. The calcium concentration in extraction solution was analyzed by inductively coupled plasma optical emission spectrometer (ICP-OES). Field-emission scanning electron microscope (FE-SEM) was also used to identify the surface morphology of calcium carbonate, and XRD was used to analyze the crystallinity and the quantitative analysis of calcium carbonate. In addition, the cement panel evaluation was carried out according to KS L ISO 679, and the compressive strength and flexural strength of the panels were measured.
Journal of The Korean Society of Grassland and Forage Science
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v.32
no.3
/
pp.309-316
/
2012
This study was performed to investigate the effects of forage cropping system and cattle slurry on productivity of whole crop rice, whole crop barley and Sorghum-Sudangrass hybrid and environmental pollution in paddy land. Forage cropping system used in this study was consisted of double-cropping whole crop barley followed by whole crop rice applied with cattle slurry (DWBRC) and double-cropping whole crop barley followed by Sorghum-Sudangrass hybrid applied with cattle slurry (DSSBC). The field experiments were conducted on the clay loam at Backsanmyun, Kimje, Chunlabukdo province in Korea for three years (May 2006 to Apr. 2009). This study was arranged in completely randomized design with three replicates. The field had been sown with whole crop rice 'Nampyung', Sorghum-Sudangrass hybrid 'Sordan79' and whole crop barley 'Younyang'. The yields of whole crop barley in DWBRC and DSSBC were 7,515 kg/ha and 8,515 kg/ha, respectively. The yields of whole crop barley in DSSBC significantly increased as compared with that of DWBRC (p<0.05). The contents of crude protein, neutral detergent fiber (NDF), acid detergent fiber (ADF), total digestible nutrient (TDN) of whole crop barley in DWBRC were not difference as compared with those of DSSBC. The pH, and contents of total nitrogen and organic matter in soil samples collected at the end of the experiment increased as compared with those at the beginning of the experiment (p<0.05). However, The content of phosphate in DWBRC was no difference as compared with DSSBC. In addition, after the end of experiment, the concentrations of exchangeable cations (Ca, Na, Mg and K) in soil samples collected at the end of the experiment were remarkably higher than those at the beginning of the experiment (p<0.05). The concentrations of $NH_4$-N, $NO_3$-N, $PO_4$-P, Cl, Ca, K, Mg and Na in leaching water were hardly influenced by the cropping system and application of cattle slurry.
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