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Safety Evaluation of Microbiological and Aflatoxin of Traditional Dried Persimmon (곶감의 미생물 및 aflatoxin에 대한 안전성 평가)

  • Seo, Min-Kyoung;Choi, Song-Yi;Lee, Kyoung Ah;Kim, Jung-Sook;Chung, Duck-Hwa;Lee, Soo-Hyung;Park, Ki-Hwan;Kim, Won-Il;Ryu, Jae-Gee;Kim, Hwang-Yong;Kim, Se-Ri
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.260-267
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    • 2014
  • To evaluate microbiological and aflatoxin safety on traditional dried persimmon, a total of 315 samples were collected from 105 farms. The collected samples were assessed on aflatoxin and microorganisms (Aerobic plate count, coliform count, Escherichia coli, Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus). The the APC of sliced dried persimmon, dried persimmon, and semi dried persimmon were $3.93{\pm}0.96$, $2.12{\pm}0.93$, and $1.50{\pm}1.08{\log}\;CFU/g$, respectively. S. aureus was detected in 40.0% of sliced dried persimmon, 29.5% of dried persimmon, and 23.5% of semi dried persimmon. E. coli recovered from dried persimmon and semi dried persimmon was 6.6%, and 2.9%, respectively. However, E. coli O157:H7, Salmonella spp., and L. monocytogenes were not detected. According to the result of aflatoxin by ELISA and UPLC, aflatoxin was not detected in any sample. These data suggested that safety management system should be introduce to the farms producing traditional dried persimmon to enhance the safety of traditional dried persimmon.

Prevalence of CTX-M-type Extended-Spectrum $\beta$-Lactamases Producing Escherichia coli and Klebsieilla pneumoniae Isolates in General Hospitals in 2005 (임상에서 분리된 CTX-M형 Extended-Spectrum $\beta$-Lactamases를 생산하는 Escherichia coli와 Klebsiella pneumoniae의 유행)

  • Kim, Yun-Tae;Kim, Tae-Un
    • Microbiology and Biotechnology Letters
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    • v.34 no.4
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    • pp.342-351
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    • 2006
  • The aim of this study was to survey susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-M type extended-spectrum $\beta$-lactamases (ESBLs) producing E. coli and K. pneumoniae in Korea. During the period of February to July, 2005, 153 E. coli and 52 K. neumoniae isolates were collected from 2 hospitals in Busan. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production of E. coli and K. pneumoniae was determined by the double disk synergy test. MICs of $\beta$-lactam antibiotics were determined by the agar dilution method. Blac$_{CTX-M}$ genes of the organism were detected by PCR. Among 153 isolates of E. coli and 52 isolates of K. neumoniae, 27 (17.6%) and 25 (48.0%) were intermediate or resistant to cefotaxime, respectively. Twenty-three (15.0%) isolates out of 153 E. coli and 13 (25.0%) out of 52 K. neumoniae isolates showed positive results for ESBL by the double disk synergy test. Twenty isolates out of 23 ESBL producing E. coli and 12 out of 13 ESBL producing K. neumoniae isolates harbored biacTx-M gene,11 of ESBL producing E. coli and 12 of ESBL producing K. neuinoniae isolates harbored bla$_{CTX-M}$ gene, 11 of the ESBL producing E. coli and 2 of ESBL producing K. neumoniae isolates harbored bla$_{TEM}$ gene, and 1 of the ESBL producing E. coli and 12 of ESBL producing K. neumoniae isolates harbored bla$_{SHV}$ gene. E. coli and K. neumoniae isolates producing CTX-M-type ESBLs were not uncommon in Korea. It is thought that continuous survey are necessary for inspecting the spread and novel variants of CTX-M-type ESBL genes. Further me]'e investigation and research on ESBL producing strains are needed in order to prevent the spread of resistant bacteria.

A New Early-Heading and High-Yielding Winter Oat Cultivar for Whole Crop Forage, 'Okhan' (조숙 다수성 추파 총체사료용 귀리 신품종 '옥한')

  • Han, Ouk-Kyu;Park, Tae-Il;Park, Hyung-Ho;Song, Tae-Hwa;Kim, Kee-Jong;Park, Nam-Geon;Ju, Jung-Il;Jang, Young-Jik;Hwang, Jong-Jin;Kwon, Young-Up
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.87-93
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    • 2013
  • 'Okhan' (Avena sativa L.), an oat cultivar for winter use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2011. It was derived from an original cross between 'Early80', exhibiting early heading, and 'Maine PI-590' (CI 7518), exhibiting large-size grain, in 1995. Subsequent generations as well as cross-bred cultivars were handled in bulk, and pedigree selection programs took place at Suwon and Yeoncheon, respectively. A promising line, 'SO95027-B-45-16-10-6-2-Y7-10', was selected in 2004, and was designated 'Gwiri74' after being selected from a yield trial for three years from 2005 to 2008. The breeding line 'Gwiri74' was subsequently evaluated for earliness of heading and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2009 to 2011, and was finally named as 'Okhan'. Over 3 years, the heading date of 'Okhan' was about 6 days earlier than that of the check cultivar 'Samhan', and their average forage dry matter yield harvested at the milk-ripe stage was 15.0 ton $ha^{-1}$, compared with 14.1 ton $ha^{-1}$ of check cultivar. Cultivar 'Okhan' was lower than the check cultivar 'Samhan' in terms of the protein content (9.2% and 9.9%, respectively) and total digestible nutrients (58.5%, and 59.3%, respectively), while the TDN yield per ha was more than the check (8.70 and 8.36 kg, respectively). Fall sowing cropping of 'Okhan' is recommended only in areas where average daily minimum mean temperatures in January are higher than $-7^{\circ}C$, and it should not be cultivated in mountain areas, where frost damage is likely to occur.

Estimation of Carbon Emission and Application of LCA (Life Cycle Assessment) from Potato (Solanum tuberosum L.) Production System (감자의 생산과정에서 발생하는 탄소배출량 산정과 전과정평가의 적용)

  • So, Kyu-Ho;Ryu, Jong-Hee;Shim, Kyo-Moon;Lee, Gil-Zae;Roh, Kee-An;Lee, Deog-Bae;Park, Jung-Ah
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.728-733
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    • 2010
  • This study was carried out to estimate carbon emission using LCA and to establish LCI database of potato production system. Potato production system was categorized into the fall season potato and the spring season potato according to potato cropping type. The results of collecting data for establishing LCI D/B showed that input of fertilizer for fall season potato production was more than that for spring season potato production. Input of pesticide for spring season potato production was much more than that for fall season potato production. The value of field direct emission ($CO_2$, $CH_4$, $N_2O$) were 2.17E-02 kg $kg^{-1}$ for spring season potato and 2.47E-02 kg $kg^{-1}$ for fall season potato, respectively. The result of LCI analysis focussed on the greenhouse gas (GHG), it was observed that carbon footprint values were 8.38E-01 kg $CO_2$-eq. $kg^{-1}$ for spring season potato and 8.10E-01 kg $CO_2$-eq. $kg^{-1}$ for fall season potato; especially for 90% and 6% of $CO_2$ emission from fertilizer and potato production, respectively. $N_2O$ was emitted from the process of N fertilizer production (76%) and potato production (23%). It was observed that characterization of values of GWP were 8.38E-01 kg $CO_2$-eq. $kg^{-1}$ for spring season potato and 8.10E-01 kg $CO_2$-eq. $kg^{-1}$ for fall season potato.

Physico-chemical Characteristics of Seasoned Pork Prepared with Medicinal Plant Extracts During Storage (한약재 추출물을 첨가한 양념 돈육의 저장 중 이화학적 특성)

  • Cho, Hee-Sook;Shin, Jung-Hye;Choi, Duk-Ju;Lee, Soo-Jung;Kang, Min-Jung;Sung, Nak-Ju
    • Journal of Life Science
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    • v.18 no.1
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    • pp.38-45
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    • 2008
  • The seasoned pork loin manufactured using with seasoning sauce that were prepared with 3 different composites of medicinal plant extracts ($T-1{\sim}3$). The the quality characteristics were investigated during storage at $4^{\circ}C$ for 15 days. Sensory evaluation was higher in medicinal plant extracts added seasoning pork, in particular, significantly highest in group T-1. Lightness ($L^*$) value tended to decrease with increasing storage days, redness ($a^*$) was significantly higher in the medicinal plant composites added groups (T-1, 2 and 3) than control, yellowness ($b^*$) had no significant difference among three groups added medicinal paint composites. In hardness test, raw pork (control) was lower than seasoned pork groups, but tended to increase by added medicinal plant composites. Cooking loss increased with increasing storage period, but there was no significant difference between the seasoned pork groups ($T-0{\sim}3$). The changes of pH showed lower in control than seasoned pork groups ($T-0{\sim}3$) added seasoning sauce. Moisture content decreased with increasing storage period. VBN content increased during storage. After storage for 15 days, it was significantly higher in the order of T-3 >> T-0 > T-2 > T-1.

The Antioxidant Activity of Yacon (Polymnia sonchifoliaty) and its Application to the Pork Patties as a Natural Antioxidant (야콘 착즙액의 항산화 활성 및 천연 항산화제로서 돈육패티에 이용)

  • Park, Jin-Sun;Kim, Hyeong-Sang;Chin, Koo-Bok
    • Food Science of Animal Resources
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    • v.32 no.2
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    • pp.190-197
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    • 2012
  • This study was performed to evaluate the extraction method (Yacon ethanol extracts; YEE, Yacon pressed extracts; YPE) and various levels (0.05-1.0%) of Yacon (Polymnia sonchifolia) on antioxidant and antimicrobial activities. In linoleic acid emulsion of YPE, there were higher iron chelation activity and antioxidant activity than those of YEE (p<0.05). A 1,1- diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and reducing power of both extracts showed a higher rate at 0.5% level. Ground pork patties, which contain 0.5% YEE and YPE, were manufactured and BHT (0.01%) was used as a reference. Physicochemical properties and microbial counts of ground pork patties, containing a different type of Yacon, were evaluated during the 14 d of storage at $4^{\circ}C$. A pH level, and lightness (Hunter L), as well as the yellowness (Hunter b) values of treatments were not different from those of the control (p>0.05), but increased during storage, at $4^{\circ}C$. Lightness values of ground pork patties, with Yacon extracts, showed the highest. TBARS value of ground pork patties that contains Yacon increased with increased storage at $4^{\circ}C$ (p<0.05), and pork patties with YPE or YEE retarded the lipid oxidation, during refrigerated storage, as compared to that of the CTL. Thus, YPE could be used as a potential possibility to inhibit the lipid oxidation of processed meats, during the refrigerated storage.

Influence of low-pressure tumbling on the quality characteristics of thawed pork (감압 텀블링 해동이 돈육의 품질 특성에 미치는 영향)

  • Won-Ho Hong;Jeong Kim;Yu-Jeong Gwak;Jiyeon Chun
    • Food Science and Preservation
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    • v.30 no.1
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    • pp.88-97
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    • 2023
  • As livestock consumption in Korea has been gradually increasing, the quality of the final products has been improved to meet this increased demand. In particular, maintaining the water holding capacity (WHC) and minimizing the drip loss during the thawing of frozen meat are of utmost importance. This study investigated the physicochemical properties of frozen pork subjected to thawing under different conditions: at room temperature (20℃, under air), at a low temperature (4℃ refrigerator, under air), under water (20℃, under water in a vacuum bag), under microwave (microwave-thawing, 260 W), and under low-pressure tumbling (20℃, 0.015 bar, tumbling). The shortest thawing time for frozen pork was recorded upon low-pressure tumbling thus indicating a fast heat transfer. The lowest drip loss (0.2%) and highest WHC (94.5%) were also recorded under this condition. A significantly higher drip loss was observed upon microwave- (1.0%) and water-thawing (1.2%), which resulted in the lowest WHC in microwave thawing (87.2%). The highest total count of aerobic bacteria and coliform group were observed upon room temp thawing while the low pressure tumbling and thawing resulted in the lowest aerobic bacteria (1.90 log CFU/g) and coliform (0.78 log CFU/g) count. Consequently, thawing by low pressure tumbling afforded the best food quality.

Preparation of Coating Agent for Fresh-Cut Fruit on Cake and Its Storage Characteristics (케이크용 신선편의 과일 코팅제의 제조 및 저장 특성)

  • Park, Jung-Eun;Yeon, Soo-Ji;Kim, Dong-Ho;Park, Yeo-Jin;Jang, Keum-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.12
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    • pp.2019-2027
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    • 2013
  • In this study, we tried to develop a coating agent for the fresh-cut fruits used in cakes. First, the coating agent mixing ratios of sugar, pectin, sodium alginate, carrageenan, xanthan gum, vitamin C, and purified water were selected to be 55, 2, 2, 0.04, 0.1, 0.05, and 40.81% (w/w), respectively. In a freeze-thaw stability of the coating agent, the viscosity remained constant for 3 cycles of freezing and thawing repetition process, but showed a slightly decreasing trend in the 4th repetition process (P<0.05). On the other hand, the sugar content, pH, and chromaticity remained constant even in the 4th repetition process. Pineapple coated with the coating agent had smaller weight loss, hardness changes, and total bacteria distribution compared to the uncoated pineapple (P<0.05). In the chromaticity, both of the two pineapples experienced browning with increasing storage duration, as L value decreases and b value increases. However, when the color difference was compared, the progress of browning for the uncoated pineapple was faster than the coated pineapple. Also, the progress of browning at $4^{\circ}C$ was found to be slower than the progress of browning at $25^{\circ}C$. Therefore, the storage stability of the fresh-cut fruits could be improved by coating the fresh-cut fruits for cakes with the coating agent and storing at a low temperature, which would contribute to extending the shelf-life of cakes.

Effect of Immature Compost on Available Nutrient Capability and Heavy Metal Accumulation in Soil for Lettuce (Lactuca sativa L.) Cultivation (퇴비 내 영양소 및 중금속이 상추 재배에 미치는 영향)

  • Phonsuwan, Malinee;Lee, Min Ho;Moon, Byeong Eun;Kim, Young Bok;Kaewjampa, Naruemol;Yoon, Yong Cheol;Kim, Hyeon Tae
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.343-350
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    • 2016
  • The aim of this study was to evaluate effects of immature compost on the amount of nutrient content, heavy metal concentration, and application rate that were used for lettuce cultivation. The characteristics of the two composts (Compost A (CA) was immature compost and Compost B (CB) was mature compost) were evaluated upon mixing with commercial soil at 0%, 25%, 50%, and 75% (w/w). The poor chemical characteristics were appeared by use of immature compost as soil amendment; the 50% and 75% rates were weakly acidic at pH 5.39 and 5.50, respectively. The total carbon content at using of 75% of the immature compost and mature compost increased the most to 14.5 and 6.5% and it significantly increased concentrations of the total nitrogen and phosphorus compared to control. As for 75% mature compost rate increased significantly the concentrations of Cu ($128mg\;kg^{-1}$), Zn ($260mg\;kg^{-1}$), Pb ($0.32mg\;kg^{-1}$) and, Cd ($0.48mg\;kg^{-1}$) compared to control, and the highest As concentration increased significantly at 75% and 50% (6.69 and $6.28mg\;kg^{-1}$) including in 25% immature compost as $6.48mg\;kg^{-1}$. However, all of the high compost rates significantly decreased the shoot biomass of lettuce. The immature compost was potentially amended at an application rate of 25% due to a slight salinity and low risk to heavy metal uptake on lettuce growth. This use may be available if the rate is lower than that used in this trial.

Studies on the Standardization of Carcass Quality Scores for Pork Quality Assurance (돈육 품질인증을 위한 도체 육질점수 표준화에 관한 연구)

  • Kim, Doo-Hwan;Seo, Jong-Tae;Kwack, Suk-Chun;Lee, Jeong-Ill
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.424-431
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    • 2007
  • This study was conducted to investigate the effect of intramuscular fat scores on pork quality assurance. Pork loins were collected from animals (110-120 kg body weight) slaughtered in a commercial slaughterhouse, assigned an IMF score (1-3) from and stored for 24 hrs at $-3^{\circ}C$. Samples were analyzed for chemical composition, pH, cooking and drip loss, shear force, meat color, and texture characteristics. The moisture, crude protein and crude ash content were not significantly different among the various IMF score groups. The crude fat content of the IMF score 3 group was significantly higher than the IMF score 1 and 2 groups (p<0.05). The pH values of the IMF score 2 and 3 groups was significantly higher than the IMF score 1 group (p<0.05). There was a no significant difference in shear force value and cooking loss among the IMF score groups. The purge loss content of the IMF 3 group was significantly lower than that of the IMF score 1 group (p<0.05). The increase in IMF score resulted in lower hardness, gumminess, and brittleness values. The hardness and gumminess of the IMF score 3 group were significantly lower than those of the IMF 1 score group. The adhesiveness, cohesiveness, and springiness were not significantly different among the IMF score groups. With regard to meat color traits, lightness ($CIE\;L^*$) was not significantly different among the IMF score groups. The $a^*\;and\;b^*$ values correlated positively with the IMF score. In general, the results of this study show that the CIE color values and drip loss had a positive correlation, while only redness was positively correlated with shear force and hardness. pH was negatively correlated with CIE color values and drip loss, while positively correlated with moisture content.