• 한국미생물·생명공학회지
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• 제17권5호
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• pp.468-474
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• 1989

Pseudomonas delafieldii이 다당류 생산을 위한 배양조건을 검토하였다. 최적 배지조성은 glucose 25g/$\ell$, peptone 2.06 g/$\ell$, yeast extract 0.5g/$\ell$, KH$_2$PO$_4$2g/$\ell$, MgSO$_4$2g/$\ell$, CaCO$_3$2.5g/$\ell$이며 교반속도는 300rpm, 온도는 30도, pH는 6.5이었다. 이 조건에서는 flask culture를 할 경우 5.91g/$\ell$가 생산되며 이 때 수율은 23.65%, overall productivity는 50.98mg/g/h이었다.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.212-217
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• 1997

A strain of Bacillus sp. WS-14 was isolated from soil. Medium optimization for ${\beta}-mannanase$ production by Bacillus sp. WS-14 was performed. Effect of various carbon sources on ${\beta}-mannanase$ production was investigated and locust bean gum was the most effective for ${\beta}-mannanase$ production. ${\beta}-mannanase$ activity and cell growth increased with increasing the concentration of locust bean gum, however, the amounts were not significant. Among nitrogen sources, soytone was the most effective for ${\beta}-mannanase$ production. Inorganic compounds such as $KH_2PO_4,\;NaCl\;Na_2CO_3\;and\;MgSO_4{\cdot}7H_2O\;on\;{\beta}-mannanase$ production were optimized for ${\beta}-mannanase$ production. Locust bean gum of 10.0 g/l, soytone of 5.0 g/l, $KH_2PO_4$ of 2.0 g/l, NaCl of 10.0 g/l, $MgSO_4{\cdot}7H_2O\;of\;0.2\;g/l,\;Na_2CO_3$, of 2.0 g/l were selected as optimum content. Production of ${\beta}-mannanase$ by using the optimum medium was carried out. The maximum ${\beta}-mannanase$ activity of 20.8 unit/ml could be obtained after 14 h fermentation which corresponed to the productivity of ${\beta}-mannanase$ of 1.48 unit/ml-h.

• 미생물학회지
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• 제40권1호
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• pp.43-48
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• 2004

본 연구에서는 우모의 생물학적 처리를 위하여 keratinolytic pretense를 생성함으로써 우모를 분해할 수 있는 Bacillus megaterium을 붕괴된 우모로부터 분리하였다. 본 균주에 의한 keratinolytic pretense생산 최적배지 조성 및 배양조건은 0.2% glucose, 0.8% skim milk, 0.05% NaCl, 0,01% $(K_2HPO_4$, 0.02%, $(KH_2PO_4$, 0.01% $MgCl_2$, 초기 pH 6.5 및 $25^{\circ}C$이었다. 특히, skim milk의 첨가는 효소 생산에 가장 효과적이었다. 최적조건에서 배양 5일만에 269 U/ml의 효소가 생산되었으며, 배양 6일경 98%의 우모가 분해되었다.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.835-843
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• 2010

The objectives of this study were to investigate the effect of fermentation medium on the hydroxyl radical scavenging activity of exopolysaccharides from Inonotus obliquus by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Corn flour, peptone, and $KH_2PO_4$ were important factors significantly affecting hydroxyl radical scavenging activity. These selected variables were subsequently optimized using path of steepest ascent (descent), a central composite design, and response surface analysis. The optimal medium composition was (% w/v): corn flour 5.30, peptone 0.32, $KH_2PO_4$ 0.26, $MgSO_4$ 0.02, and $CaCl_2$ 0.01. Under the optimal condition, the hydroxyl radical scavenging rate (49.4%) was much higher than that using either basal fermentation medium (10.2%) and single variable optimization of fermentation medium (35.5%). The main monosaccharides components of the RSM optimized polysaccharides are rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar proportion at 1.45%, 3.63%, 2.17%, 15.94%, 50.00%, and 26.81%.

• 한국식품과학회지
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• 제13권4호
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• pp.255-260
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• 1981

비타민과 무기염류가 팽나무 버섯균(菌)의 균사생육(菌絲生育)과 자실체형성(字實體形成) 미치는 영향에 대하여 실험하였다. 비타민 중에서는 티아민이 제일 우수하였고 그 최적 농도는 $50{\mu}g%$이었으며, 무기 염류 중에서 $KH_{2}PO_{4}$의 최적농도는 0.2%, $MgSO_{4}$의 최적농도는 0.02%이었으며 그 밖의 무기염류를 단용 또는 혼용시에 균사(菌絲)와 자실체(字實體)의 수율에 효과가 없었다.

• KSBB Journal
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• 제11권5호
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• pp.550-556
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• 1996

Acetobacter xylinum A TCC 23769에 의한 미생물 생룰로오스의 생산을 위한 최적배지가 개발되었다. 미생물생룰로오스의 생산을 위한 각 영양성분의 최 척농도는 리터당 10 g peptone, 20 g yeast extract, 5g glucose, 1.56 g $Na_2HPO_4$, 1.8 g$KH_2PO_4$, 0.05 g $MaSO_4$, 0.002g $FeCl_3$, 5 g citric acid 그리고 10 mL ethanol로 결정되었다. 개발된 배지에서 수득한 생룰로오스의 생산성은 구연산과 에탄융의 상승효과 로 정치배양시 보고된 결과보다 매우 높은 포도당 단위그람당 0.446 그람이었다. 개발된 배지에서 정치배양시 세포성장 빛 셀룰로오스의 생산 또한 조사 되었다.

• 한국균학회지
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• 제17권3호
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• pp.149-153
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• 1989

내산성 ${\alpha}$-아밀라제를 강력히 생산하는 Aspergillus niger K-25 균주를 토양으로부터 분리하였다. 내산성 아밀라제 효소를 생산하기 위한 균주의 배양조건을 조사하였다. 합성배지 A(soluble starch 3.5%, peptone 2%, $KH_2PO_4$ 0.5%, $MaSO_4{\cdot}7H_2O$ 0.25%, $FeCI_3$ 1.0%, pH 6)에서 배양시 아밀라제 생산이 높았다. 밀기울에 fumaric acid 완충용액(pH3)을 1 : 1(W/V)로 첨가하여 배양했을 때 내산성 ${\alpha}$-아밀라제의 생산력은 완충액 HCI, citric acid과 밀기울만 넣은 배지보다 약 2배가 높았고, 배지 A보다 현저하게 높았다.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.433-439
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• 1999

Among microorganisms isolated from soil, YJ-66 strain was the best producer of flocculant and was examined for flocculating ability in the active carbon and CaCl2. YJ-66 strain was the best producer of flocculant and was examined for flocculating ability in the active carbon and CaCl2. YJ-66 strain was identified to be a species belonging to the genus Achromobacter. The optimum culture condition for production of bioflocculant with the isolated strain was for 72hrs at 3$0^{\circ}C$ and pH7.5. The favorable carbon, nitrogen sources and inorganic salts for production of the flocculant were sucrose, peptone, MgSO4 and KH2PO4, whose optimal concentrations were 2%. 0.067%, 0.1% and 0.1%, respectively. Addition of the carbon and inorganic salts significantly increased the production of flocculant. Compositions of optimized culture medium for bioflocculant production by Achromobacter sp. YJ-66 were 2% sucrose, 0.067% peptone, 0.1% MgSO4 and 0.1% KH2PO4 in initial pH 7.5 during at 3$0^{\circ}C$ for 72hrs.

• 자연과학논문집
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• 제8권1호
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• pp.33-39
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• 1995

Klebsiella sp. L-10을 N-methyl-N'-nitro-N''-nitrosoguanidine 과 ethylmethane sulfornate 및 자외선 등으로 돌연변이시켜 히아루론산 복합체의 수율이 친주보다 약 2.5배 증대된 NTG 50 변이주를 얻었다. NTG 50변이주를 이용한 히아루론산 복합체의 최적 생산배지 조성은 효모 추출물 0.1%, 트립톤 3%, 포도당 3%, $K_2HPO_4$ 와 $KH_2PO_4$ 각각 30nM 이었고 배양조건은 배지의 초기 pH 6.0-6.5, 배양온도 $37^{\circ}C$, 24시간의 진탕배양이었으며 이때 배양액 리터당 2900mg의 히아루론산 복합체가 생산되었다.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.311-316
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• 1997

A high xylitol producing yeast was isolated from the sludge of xylose manufacturing factory and then identified as Candida tropicalis DS-72 according to physiological properties. The strain was able to produce xylitol in a high concentration up to 72g/l from 100g/l xylose in 32 hours. Medium optimization for xylitol production by C. tropicalis DS-72 was performed. Effect of various nitrogen sources on xylitol production was investigated. Of nitrogenous compounds, yeast extract was the most suitable organic nitrogen nutrient for the enhancement of xylitol production. However, inorganic nitrogen resulted in a low cell concentration and did not produce xylitol. Effect of inorganic salts such as KH$_{2}$PO$_{4}$, and MgSO$_{4}$, 7H$_{2}$O on xylitol production was also studied. Optimal medium was selected as xylose 100g/l, yeast extract 10g/l, KH$_{2}$PO$_{4}$, 5 g/l and MgSO$_{4}$, 7H$_{2}$O 0.2 g/l. Xylitol of 88 g/l was produced from 100 g/l xylose in 30 hours using the optimal medium in a flask. In a fermentor, a fed-batch culture with 300g/l xylose was carried out. A final xylitol concentration of 240 g/l in the culture could be obtained in 43 hours of culture time by maintaining the high level of dissolved oxygen during growth phase and limiting the dissolved oxygen in the same culture during production phase. This result corresponded to a xylitol yield of 80% and a xylitol productivity of 5.58 g/1-h.