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Production of a Keratinolytic Protease by a Feather-Degrading Bacterium, Bacillus megaterium F7-1  

손홍주 (밀양대학교 생물공학과)
박근태 (제주대학교 해양과학)
김용균 (밀양대학교 생물공학과)
Publication Information
Korean Journal of Microbiology / v.40, no.1, 2004 , pp. 43-48 More about this Journal
Abstract
Bacillus megaterium F7-1 producing keratinolytic protease was isolated from decayed chicken feather. The optimal culture conditions for the production of keratinolytic protease by B. megaterium F7-1 were investigated. The composition of optimal medium for the keratinolytic protease was 0.2% glucose, 0.8% skim milk, 0.05% NaCl, 0.01 % $(K_2HPO_4$, 0.02%, $(KH_2PO_4$ and 0.01 % $MgCl_2$. Especially, skim milk was found to be the most effective compound in keratinolytic protease production. The optimal temperature and initial pH were 6.5 and $25^{\circ}C$, respectively. The keratinolytic protease production under optimal condition reached a maximum of 269 U/ml after 5 days of cultivation. B. megaterium F7-1 degraded 98% of the feather used in the optimized medium within 6 days.
Keywords
Bacillus megaterium; feather; keratin; keratinolytic protease;
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