• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.571-584
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• 2004

It is known widely that granulosa cell apoptosis leads follicular atresia and macrophages exert their effects directly and/or indirectly from the initiation to the completion of follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. However, the site of initiation, propagation routes and the elimination methods of apoptotic bodies, and the time and methods of penetration of macrophages into the follicles are not known completely. Using pig(Yorkshire-breed) ovary, immunohistochemical studies with TUNEL for apoptotic bodies and pig macrophage monoclonal antibody 4E9 for macrophages, and light and transmission electron microscopic observations were performed. In the pig, follicular atresia began with the granulosa cell apoptosis, and the apoptosis of theca intema cells occured at the same time. The apoptosis of granulosa cells initiated randomly within the granulosa cell layer and propagated rapidly into the whole layer. Ultrastructura1ly, apoptotic granulosa cells showed characteristic pyknotic and deformed nucleus and intracytoplasmic vesicles. Apoptotic bodies were eliminated by intact granulosa cells and macrophages. Intact granulosa cells ingested apoptotic bodies transiently, soon after they fell into the apoptosis. Finally, apoptotic bodies and degenerated oocyte were phagocytosed by macrophages. Macrophages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progression of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia These results will provide valuable informations on the study of the interrelation between macrophage and ovarian follicular atresia.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.603-612
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• 2004

This study was carried out to investigate the replacing fish meal or soybean meal effect of with feather meal or Bacillus sp. inoculated feather meal in broiler diets on the performances and nutrient utilization. One hundred and eighty broilers were randomly allotted to six dietary treatments(1) control, basal diet; (2) BFMl00, Fish meal replacing at 100% level with bacillus sp. inoculated feather meal; (3) BSM20, Soybean meal replacing at 20% level with bacillus sp. inoculated feather meal; (4) BFMl00+BSM20, Fish meal and soybean meal replacing at 100% level and 20% level with bacillus sp. inoculated feather meal, respectively; (5) GFMl00, Fish meal replacing at 100% level with general feather meal; and (6) GSM20, Soybean meal replacing at 20% level with general feather meal) in a 5 week feeding trial. In overall period, body weight gain of control was the highest(1,623g) and those of BFM 100, BFM 100+ BSM 20 and GFM 100 were 1,572g, 1,564g, and 1,078g, respectively. And that of GFM 100(1,078.3g) was the lowest(p<0.05) among treatments. Digestibility of dry matter for BFM 100+BSM 20(81.46%) was higher(p<0.05) than that for other treatments, and that of crude protein was the lowest (p<0.05). Digestibilities of organic matter were significantly(p<0.05) improved when Bacillus sp. inoculated feather meal was replaced at 100% level with fish meal in the basal diet. Methane and hydrogen sulfide gases from the feces were significantly(p<0.05) decreased in chicks fed the control, BFM 100 and GFM 100 diets, when observed after 3 weeks of feeding trials. Feed costs of the control and BFM 100 were 417 and 384 won, respectively but that of BSM 20 was 558 won. Therefore, replacement of fish meal with Bacillus sp. inoculated feather meal in the diets for chicks could be useful for economic production.

• Applied Biological Chemistry
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• v.15 no.2
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• pp.169-173
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• 1972

In five varietes of Citrus junos sieb grown in different area in Korea, the general components, total acids and inorganic components were calculated and compared with one other. In the orchard soils, the physical and chemical properties were studied. The results are summarized as follow: 1. Generally, the rind was weighter than tile flesh compared with the other fruits and the proportion of the rind was 49%. 2. Total contents of organic acids in rind and flesh were 12.29me and 39.79me respectively. 3. The order of the contents of inorganic components in the flesh and rind was $CaO>K_2O>MgO>P_2O_5>SO_4$ 4. The contents of crude protein and crude fat were increased with those of MgO and $SO_4$ in fruits. 5. The quantity of protein in the soil seed to influence greatly in rind formation not only in citrus fruits but also Citrus junos sieb. 6. In growing Citrus junos sieb, C.L. seemed to he the most adequate soil. 7. The pH range of the soil was from 5.05 to 7.20 generally. 8. The contents of crude protein and crude fat in citrus fruits seemed to be increased with those of organic material and total-nitrogen in the soil.

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.13-20
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• 2006

The purpose of this study was to develope the transgenic cattle expressing hFSH into the urine using the nuclear transfer. To produce the interest gene in urine, the specific vector was ligated with hFSH gene undo. maUII promoter. The fetal fibroblast cells (KbFF) were isolated from a 45-day male fetus. The hFSH gene was co-transfected with pcDNA3 (neo) vector to KbFF cells by electroporation. The gene-transfected cells were cultured with G-418 selection medium for 2 weeks. Selected colonies were confirmed by PCR. For nuclear transfer, enucleated bovine oocytes were transferred with hFSH transfected or nontransfected fetal fibroblasts. The cleavage and blastocyst formation rates were significantly lower (p<0.05) in cloned embryos transfected with hFSH gene (68.7% and 15.7%) than in those non-transfected (67.6% and 24.5 %), respectively. Apoptosis analysis showed no difference between hFSH transfected and non-transfected blastocysts (p>0.05). The blastocysts were transfected to 77 (control 24, hFSH 53) recipient cows. Two calves were born (1.9%) following transfer with NT embryos transfected with hFSH gene, but they were confirmed not to be transgenic calves. This result shows that the hFSH colonies were mixed with transfected and non transfected cells. Further research will be needed for selection and establishment of gene transfected cells.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.131-136
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• 2002

A field experiment was carried out to compare the nitrate nitrogen concentration, sugar content and animal intake of low $sorghum{\times}sudangrass$ hybrids (Sorghum bicolor (L.) Moench) grown under application of animal manure in 1995. The application amount of animal manure were 40MT in cattle manure, and 30MT/ha in swine and poultry manure. The four NLCF's recommended hybrids used in this study were Pioneer 988, TE-Haygrazer and NC+ 855 (heading type), and Jumbo(headless type). The nitrate nitrogen concentration of forage was 365mg/kg at 50~60cm of plant height, 501mg at 100~120cm, and 502mg at 200~220cm, regardless of animal manure type and cultivar. The nitrate nitrogen concentration of NC+ 855 and P 988 was 519 and 526mg, respectively, and were higher than that(317mg) of Jumbo(P<0.05). The nitrate nioogen concentration of stems was 376mg, and significantly higher than that(135mg) of leaves(P<0.05) regardless of cultivar, and lower parts of stems and leaves were higher than those of upper parts of plants. The sugar contents of four $sorghum{\times}sudangrass$ hybrids were ranged between 3.1 and $3.7^{\circ}$ in cattle manure, 2.9 and $3.3^{\circ}$ in swine manure, and 2.8 and $4.9^{\circ}$ in poultry manure. The sugar content of NC+ 855 was the highest as $3.9^{\circ}$, p 988 was second as $3.4^{\circ}$, and then TE-Haygrazer and Jumbo were lower as $3.2^{\circ}$ and $2.9^{\circ}$, respectively. Animal intake of sorghum ${\times}$ sudangrass hybrids P 988 and NC+ 855 were slightly higher than those of Jumbo and TE-Haygrazer grown under application of cattle manure. Also the average intake of P 988 was a little higher than that of Jumbo. However, there were no significant differences of forage intake among few recommended $sorghum{\times}sudangrass$ hybrids.

• Proceedings of the KSAR Conference
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• 2005.06a
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• pp.47-54
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• 2005

In boars, unlike the cases in males of other species, gonadal hormones suppress voluntary feed intake. for this reason, barrows, compared with gilts or boars, eat too much feed resulting in excessive fat deposition. Two experiments were performed in the present study to investigate the effects of implantation of Revalor H[Experiment(Exp.) I: 140mg trenbolone acetate(a synthetic androgen) + 14mg estradiol-$17\beta(E_{2}\beta)$] and Compudose(Exp. II; 24mg $E_{2}\beta$) on growth efficiency, carcass characteristics and circulating concentrations of IGF-I and IGF-binding protein-3(IGFBP-3). In Exp. I, sixty-four cross-bred finishing barrows weighing approximately 60kg were randomly divided into eight pens under a 2[control vs Revalor implant] $\times$ 2(ad libitum vs $80\%$ ad libitum feeding) $\times$2[control($103\%$ NRC-recommended level) vs low-energy($87\%$ NRC recommendation) diet] arrangement of treatments. In Exp. II, effects of Compudose were studied using 80 finishing barrows(10 animals/pen). In both Exps., all the animals were slaughtered at 100- to 110-kg body weight. Both Revalor and Compudose implants caused a decrease in feed intake and backfat thickness without affecting major physicochemical characteristics of the carcass and an increase in circulating IGF-I concentration. Moreover, Revalor implant exhibited greater effects than restricted feeding, low-energy diet, or Compudose in these variables. In addition, Revalor implantation suppressed weight gain, but enhanced the feed efficiency without exhibiting any interaction with the diet or feeding. In summary, results suggest that 1) both androgen and estrogen suppress voluntary feed intake and backfat deposition and enhance IGF-I secretion and 2) these effects of the gonadal steroid hormones in growth are likely to be mediated, in part, by IGF-I in finishing barrows.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.703-710
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• 2003

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of $\beta$-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F$_2$ population composed of 214 individuals from an intercross between Korean Native Boars and Landrace sows. PCR products from two primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and HinfⅠ, revealed fragment length polymorphisms(RFLPs). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP/HinfⅠ was .38, .41 and .20, respectively, in the population. Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weights at 3, 5, 12, and 30 week of age (p〈.05 to .001). The ‘d’ allele was associated with gaining of body weight. In H-FABP/HinfⅠ locus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p〈.05 or p〈.001) and back fat thickness, body fat including abdominal and trimmed fat (p〈.001) and intramuscular fat(p〈.05) The ‘H’ allele was positively associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.875-884
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• 2003

Operational parameters for struvite crystallization, as a process to recover nitrogen and phosphorus resources from animal wastewater, were studied in this research. Crystallization distinctive of NH$_4$-N and PO$_4$$^{3-}$ in accordance to chemical sources, influent pH, aeration and stirring was examined using 2L of working volume of struvite reactor. Also, to find an effective treatment process combining with electrolysis method, removal characteristics of NH$_4$-N and PO$_4$$^{3-}$ in 6 different processes was tested. As chemical sources for the derivation of struvite formation, MgSO$_4$ and MgCl$_2$ were superior to CaCO$_3$ and CaCl$_2$. From experiment which was conducted to know the effects of aeration and stirring on struvite formation, it was revealed that aeration stimulated the crystallization reaction by inducing faster pH increase. While 90% of P removal was achieved within 1 hour under aeration, 14 hours was consumed under stirring condition. Struvite formation under aeration was affected by influent pH. No crystallization was observed at pH 5 level, but active crystallization reaction was induced over pH 6.0. 95% of P removal by struvite formation at pH 6, 7 and 9 was achieved within 3h, 2h and 10 min., respectively. However, over pH 10, operational problem due to excessive foam formation occurred, and blunting of crystallization reaction was observed at pH 11. When consider the pH range of animal wastewater, pH 7 to 9, efficient struvite formation could be achieved by simple aeration, without any chemical usage for pH adjustment. Among tested processes, the treatment process which electrolyzing the supernatant from struvite reactor, providing air to both reactors, showed best pollutant removal efficiencies. In this combined process, the removal efficiencies of NH$_4$-N and PO$_4$$^{3-}$ was 86% and 98%, respectively, and 92.4% of color removal was obtained.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.767-776
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• 2003

An experiment was conducted to investigate the effects of dietary herbal product(Miracle$^{\circledR}$) supplementation on the performance, nutrient digestibility, fecal microflora and blood parameters in pigs. Forty eight cross bred(Y${\times}$L${\times}$D) weanling pigs (average initial body weight 7.84kg${\pm}$0.17, 28 d old) were used in 35 d feeding trial. Pigs were allotted to 16 raised floor cages(W 35.5cm${\times}$L45cm${\times}$H 55cm). Each treatment had four replications of 3 pigs each. Treatments were: control (T1), CTC 100ppm (T2), Miracle$^{\circledR}$ 0.15% (T3) and CTC 100ppm + Miracle$^{\circledR}$ 0.15% (T4). The herbal product supplementation(T3) tended to improve the weight gain, feed intake, feed/gain but there were no significant differences among treatments. Nutrient digestibility was significantly(P〈0.05) affected by dietary treatments. The pigs fed T3 diet showed the lowest digestibility of DM, crude protein, NFE, P, and amino acids among treatments. Serum cholesterol level of pigs fed T3 diet was lowest and significantly(P〈0.05) lower than those fed antibiotic supplemented diet(T2). Triglyceride and HDL level of the pigs fed T3 diet were also lower than those of others but the differences were not significant. The level of serum IgG was significantly(P〈0.05) different among treatments. The level of serum IgG was highest in T4, followed by T1, T2 and T3. Serum AST(Aspartate aminotransferase) level of the control(T1) was lower than other treatments. Levels of serum total protein, albumin and ALT(Alanine aminotransferase) were not significantly different among treatments. The colony forming units (CFU) of Lactobacillus, Cl. perfringens and E.coli in feces were not significantly different among treatments but those of Cl. perfringens of T2 (CTC 100ppm) showed the lowest count at both 3rd and 5th wk. It is concluded that herbal product Miracle$^{\circledR}$ does not significantly affect growth performance of weanling pigs but it influenced the digestibility, serum IgG, cholesterol and AST level. The combination of the herbal product Miracle$^{\circledR}$ with antibiotic(CTC) showed no synergistic effects.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.679-688
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• 2003

The leptin receptor gene(LEPR) produces a high affinity receptor that mediates the regulation of the leptin gene. Leptin secreted from adipose tissue plays an important role in regulating feed intake and energy balance. In this study, a microsatellite marker within LEPR was selected and genotyped for the F2 population composed of 354 individuals from an intercross between Korean Native boars and Landrace sows. Totally, six alleles (255, 259, 261, 263, 265 and 267bp) and nineteen genotypes were detected in the population, of which the CE (261/265), CC (261/261) and EE (265/265) types were observed by 20.0%, 10.1% and 9.6%, respectively. Relationships between their genotypes and economic traits were analyzed. We found specific genotypes associated with economic traits such as body weight at 12 weeks of age/body fat including abdominal and trimmed fat/shear force (P〈0.001), body weight of 30 weeks of age (P〈0.01) and body weight of 3 weeks of age/back fat thickness (P〈0.05). The DD (263/263) and DF (263/267) types were associated with body weight at 3, 5, 12 and 30 weeks of age. The DF (263/267) type showed a highly significant effect on back fat thickness and body fat including abdominal and trimmed fat. The DF (263/267) type showed positive effect on shear force, whereas the BB (259/259) and DD (263/263) types negatively affected on tenderness.