• 한국통신학회논문지
• /
• 제36권7C호
• /
• pp.481-487
• /
• 2011

CDD(Cyclic Delay Diversity)는 다중 전송 안테나를 사용하는 OFDM(Orthogonal Frequency Division Multiplexing) 시스템에서 간단히 전송 다이버시티를 얻을 수 있는 기법이다. 그러나 CDD 안테나가 적용된 OFDM 시스템에서 형성되는 채널의 주파수 선택적 특성은 정수배 주파수 옵셋(Integer Frequency Offset)과 FFT(Fast Fourier Transform) 이후의 동기화 기법들의 성능 감쇄를 발생시킨다. 본 논문은 CDD를 갖는 OFDM 기반의 DRM+ 시스템을 위한 향상된 정수배 주파수 옵셋 추정 기법을 제안한다. 제안된 기법에서는 채널의 주파수 특성이 동일한 파일롯 부분 집합을 선택함으로써 CDD에 형성되는 채널의 주파수 선택적 특성에 의한 정수배 주파수 옵셋 추정의 열화 성능을 감소시킨다. 모의실험 결과를 통해 제안된 기법의 정수배 주파수 옵셋 추정 성능이 기존 기법에 비해 향상됨을 보인다.

• 한국양식학회지
• /
• 제22권1호
• /
• pp.83-91
• /
• 2009

The Korean doty barbel Hemibarbus mylodon (Cypriniformes; Cyprinidae) is a critically endangered freshwater fish species mainly because of its natural habitat degradation. Three full-length complementary DNA (cDNA) clones representing different muscle actin isoforms were isolated and characterized. The three muscle actin isoforms were 1,294-1,601 bp long with the identical open reading frames of 1,134 bp with the deduced amino acid residues of 377. They showed 83.9-87.2% identities in the coding nucleotide level and 96.8-98.1% identities in the amino acid level. Phylogenetic analysis with the coding nucleotide sequences revealed that three muscle actin isoforms of H. mylodon formed strongly supported monophyletic groups with one of cypriniform skeletal $\alpha$-actin (acta1), cypriniform aortic $\alpha$-actins (acta2), and uncharacterized Danio rerio muscle actin isoform/Salmo trutta slow muscle actin (a novel muscle actin type). Our phylogenetic tree further suggested that cypriniform acta2 only showed the orthologous relationship to tetrapod acta2. Other multiple actin isoforms from diverse teleostean taxa were however clustered to no tetrapod orthologs, i.e., acta1, cardiac $\alpha$-actins (aetc1), acta2, and enteric $\gamma$-actin (actg2). This result strongly suggested that teleostean muscle actins have experienced different and complicated evolutionary history in comparison to mammalian counterparts.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권3호
• /
• pp.358-365
• /
• 2013

A series of in vitro studies were carried out to determine i) the effects of enzyme and formaldehyde treatment on the degradation characteristics of carbohydrate and protein sources and on the synchronicity of these processes, and ii) the effects of synchronizing carbohydrate and protein supply on rumen fermentation and microbial protein synthesis (MPS) in in vitro experiments. Untreated corn (C) and enzyme-treated corn (EC) were combined with soy bean meal with (ES) and without (S) enzyme treatment or formaldehyde treatment (FS). Six experimental feeds (CS, CES, CFS, ECS, ECES and ECFS) with different synchrony indices were prepared. Highly synchronous diets had the greatest dry matter (DM) digestibility when untreated corn was used. However, the degree of synchronicity did not influence DM digestibility when EC was mixed with various soybean meals. At time points of 12 h and 24 h of incubation, EC-containing diets showed lower ammonia-N concentrations than those of C-containing diets, irrespective of the degree of synchronicity, indicating that more efficient utilization of ammonia-N for MPS was achieved by ruminal microorganisms when EC was offered as a carbohydrate source. Within C-containing treatments, the purine base concentration increased as the diets were more synchronized. This effect was not observed when EC was offered. There were significant effects on VFA concentration of both C and S treatments and their interactions. Similar to purine concentrations, total VFA production and individual VFA concentration in the groups containing EC as an energy source was higher than those of other groups (CS, CES and CFS). The results of the present study suggested that the availability of energy or the protein source are the most limiting factors for rumen fermentation and MPS, rather than the degree of synchronicity.

• 대한화장품학회지
• /
• 제48권1호
• /
• pp.11-24
• /
• 2022

Piper methysticum 뿌리의 에탄올 추출물은 멜라닌 세포 자극 호르몬 활성화 B16 흑색종 세포에서 멜라닌 생성을 억제하는 것으로 알려져 왔다. 이 추출물로부터 분리한 flavokawain B (FKB)와 flavokawain C (FKC)는 항멜라닌 생성 활성을 기반으로 멜라닌 생성을 억제하는 것으로 밝혀져 있다. 본 연구는 melan-a 세포에서의 멜라닌 합성에 대한 FKB 및 FKC의 억제 및 그 과정을 알아보고자 하였다. FKB와 FKC는 각각 10 μM, 5 μM 농도에서 melan-a 세포의 멜라닌 생성을 억제하였다. 그러나 FKB와 FKC 모두 세포 외 타이로시네이즈 활성은 억제하지 않았다. FKB는 타이로시네이즈 (tyrosinase, Tyr), 타이로시네이즈 연관 단백질 1 (tyrosinaserelated protein 1, TRP-1)과 2(tyrosinase-related protein 2, TRP-2), microphthalmia-associated transcription factor (MITF)의 단백질 발현량을 감소시켰으며 Tyr와 TRP-1의 mRNA 발현량을 감소시켰다. FKC는 TRP-2와 MITF의 단백질 발현량을 감소시켰으며 Tyr와 TRP-1의 mRNA 발현량을 감소시켰다. Tyr와 TRP-1의 감소된 발현은 주요 멜라닌 생성 단백질을 조절하는 MITF발현 감소로 인한 것임을 예상할 수 있었다. 다만 MITF의 mRNA 발현량은 FKB와 FKC 처리에 의해 변하지 않았기 때문에, MITF의 분해를 조절한다고 알려진 세포 외 신호 조절 키나아제(ERK)/AKT 인산화에 대한 FKB와 FKC의 영향을 확인하였다. FKB와 FKC는 AKT의 인산화를 증가시키지는 않았지만 ERK1/2의 인산화를 유의미하게 증가시켰다. 이러한 결과는 FKB와 FKC가 다양한 색소 침착 증상들에 대한 잠재적인 미백제로 도움이 될 수 있음을 말해준다.

• 전자공학회논문지D
• /
• 제34D권3호
• /
• pp.60-73
• /
• 1997

By introducing a compressive-strained quanternary InGaAsP quantum-wells instead of a conventional ternary InGaAs quantum-wells in 1.55.mu.m DFB-LD, the lasing performances canb e improved and the problems caused by the thickness non-uniformity and the compositional abruptness among the hetero-interpaces canb e relaxed. In this paper, we investigated an iptimum InGaAsP/InGaAsP multiple-quantum-well(MQW) structure as an active layer in a direct-modulated 1.55.mu. DFB-LD from the view point of threshold current, chirping charcteristics, and resonance frequency. The optimum compressive-strained MQW structure was revealed as InGaAsP/InGaAsP structure with strain amount of about 1.2%, number of wells $N_{w}$ of 7, well width $L_{w}$ of 58.agns.. The threshold current density J of 500A/c $m^{2}$, the linewidth enhancement factor a of 1.8, and differential resonance frequency of d $f_{r}$/d(I-I)$^{1}$2/=2GHz/(mA)$^{1}$2/(atI=2 $I_{th}$) were expected in 1.55.mu.m .gamma./4-shifted DFB-LD with the cavity length of 400.mu.m long and kL value of 1.25. These values are considerably improved ones compared to those of 1.55um DFB-LD with InGaAs/InGaAsP MQW which have enhancement factor and the resonance frequence frequency by the detuning of lasing wavelength and gain-peak wavelength. It was found that the linewidth enhancement factor of 20% and differential resonance frequency of 35% without the degradation of the threshold current density could be enhanced in the range of -15nm~-20nm detuning which can be realized by controlling the thickness and Incomposition of InGaAsP well. well.and Incomposition of InGaAsP well. well.

• 콘크리트학회논문집
• /
• 제18권5호
• /
• pp.667-675
• /
• 2006

대분분의 콘크리트 구조물은 여러 가지 환경요인에 노출되었는데, 여러 가지 환경요인 중, 요인 한가지만의 작용이 아니라 여러 가지 요인이 복합적으로 작용하여 콘크리트의 내구성은 현격하게 저하하게 된다. 본 연구에서는 복합양생을 받는 폴리머 시멘트 모르타르에 관한 강도 및 내구성 개선효과를 실험하였다. 폴리머 종류, 폴리머 시멘트비, 시멘트-잔골재비를 변화하여 폴리머 시멘트 모르타르를 제작한 후, 5가지의 환경요인, 즉 양생조건을 달리하여, 강도성상, 중성화, 염화물이온 침투, 내동해성, 내산성을 시험하였다. 시험결과, 폴리머 시멘트 모르타르는 보통 시멘트 모르타르에 비해 우수한 강도성상을 나타냈으며, 양생조건 II와 V에서 강도가 개선되었으나, 양생조건 I와 IV에서는 강도의 발현이 낮았다. 또한 중성화 및 염화물이온 침투 깊이는 양생조건 III-C, IV-B, V-A 순으로 작아졌으며, 폴리머 시멘트비의 증가에 따라 감소하였다. 본 연구에서 폴리머 시멘트비의 적정 혼입비는 10% 또는 15%이었다.

• 대한화학회지
• /
• 제7권2호
• /
• pp.96-105
• /
• 1963

The weight decrease curves of 18 kinds of polymers have been measured by thermobalance at the same condition where temperature is increased $1^{\circ}C$ per minutes under nitrogen or air atmosphere. The curves are further differentiated to obtain rate curve of weight decrease. Those curve offer a method to compare relative thermal stability, effects of oxygen or modes of thermal degradation of polymers qualitatively. The curves could be classified into following four types: Polystyrene, polymethylmethacrylate and acetal polymer belong to the first type. Those polymers depolymerize mainly into corresponding monomers, weight decrease curves are steepy up to perfect vaporization of polymers and rate curves show a relatively sharp peak. (Type I) Polyvinyl chloride represents the second type. This polymer decomposes with splitting off of hydrogen chloride. The thermogravimetric curve rises rapidly at first, then level off at the moderate weight decrease and gradually rises. Polyvinyl acetate also belongs to this class. (Type II) The modification of the second type is represented by polyester. The curve at the early stage is less steep, the leveling off at the next stage is less clear and the final rising of the curve is steeper than the normal second type. Polyamide, polyurethane, and polycarbonate belong to this type. (Type II') The thermal decomposition of the third type polymers is more complex than that of others. Various irregular chain scissions including side chain splitting and depolymerization to monomers occur simultaneously. The weight of the polymer decreases gradually and the rate curve does not show sharp peaks. Polyvinyl alcohol and diene polymers belong to this type. (Type III) Generally, polycondensation polymers are more stable toward heat than addition polymers and polymers having aromatic nucleus show good thermal stability. Polymers having tertiary carbon atoms such as polystyrene or polypropylene and acetal resin start decomposition under airatmosphere at the temprature below $50^{\circ}C$ or more of the temperature where the polymers start decomposition under nitrogen atmosphere.

• Parasites, Hosts and Diseases
• /
• 제60권5호
• /
• pp.345-352
• /
• 2022

Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)₈ triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40℃ and pH values between 4-7. Enzyme activity was inhibited by Zn2⁺, Ca2⁺, and Fe3⁺, whereas Mg2⁺ and K⁺ potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.

• 한국환경성돌연변이발암원학회지
• /
• 제25권2호
• /
• pp.51-59
• /
• 2005

Overexpression of HSP25 delayed cell growth, increased the level of $p21^{waf}$, reduced the levels of cyclin D1, cylcin A and cdc2, and induced radioresistance in L929 cells. We demonstrated that extracellular regulated kinase (ERK) and MAP kinase/ERK kinase (MEK) expressions as well as their activation (phospho-forms) were inhibited by hsp25 overexpression. To confirm the relationship between ERK1/2 and hsp25-mediated radioresistance, ERK1 or ERK2 cDNA was transiently transfected into the hsp25 overexpressed cells and their radioresistance was examined. HSP25-mediated radioresistance was abolished by overexpression of ERK2, but not by overexpression of ERK1. Alteration of cell cycle distribution and cell cycle related protein expressions (cyclin D, cyclin A and cdc2) by hsp25 overexpression were also recovered by ERK2 cDNA transfection. Increase in Bc1-2 protein by hsp25 gene transfection was also reduced by subsequent ERK2 cDNA-transfection. In addition, HSP25 overexpression reduced reactive oxygen species (ROS) and increased expression of manganese superoxide dismutase (MnSOD) gene. Increased activation of NF-kB (IkB degradation) was also found in hsp25-overexpressed cells. Moreover, transfection of hsp25 antisense gene abrogated all the HSP25-mediated phenomena. To further elucidate the exact relationship between MnSOD induction and NF-kB activation, dominant negative $I-kB\alpha(I-kB\alpha-DN)$ construction was transfected to HSP25 overexpressed cells. $I-kB\alpha-DN$ inhibited HSP25 mediated MnSOD gene expression. In addition, HSP25 mediated radioresistance was blocked by $I-kB\alpha-DN$ transfection. Blockage of MnSOD with antisense oligonucleotides in HSP25 overexpressed cells, prevented apoptosis and returned the ERK1/2 activation to the control level. From the above results, we suggest for the first time that reduced oxidative damage by HSP25 was due to MnSOD-mediated down regulation of ERK1/2.

• Journal of Acupuncture Research
• /
• 제32권3호
• /
• pp.27-40
• /
• 2015

Objectives : This study was performed to evaluate the effects of water extract of Cervi Parvum Cornu(CPC), Aconiti Lateralis Radix Preparata(ALR), and Yongbu-tang(YBT) on suppression of the receptor activator of nuclear factor kappa-B ligand(RANKL)-induced osteoclast differentiation and bone resorption. Methods : The effects of CPC, ALR, YBT extracts on osteoclast differentiation were determined by culture of bone marrow macrophage(BMM). The mRNA expression levels of the nuclear factor of activated T-cells cytoplasmic 1(NFATc1), c-Fos and tartrate-resistant acid phosphatase(TRAP) in BMMs were analyzed by reverse transcriptase polymerase chain reaction(RT-PCR). Similarly, the protein expression levels of NFATc1, c-Fos, mitogen-activated protein kinase(MAPK)s and ${\beta}$-actin in cell lysates were measured by western blotting. In addition, effects of CPC, ALR and YBT extracts were determined by means of Lipopolysaccharide(LPS)-induced bone-loss with mice. Results : CPC, ALR and YBT extracts showed remarkable inhibition on RANKL-induced osteoclast differentiation without cytotoxicity. CPC and ALR extracts significantly reduced the protein expression level of NFATc1. YBT extract significantly reduced the mRNA expression levels of c-Fos, NFATc1 and the protein expression levels of c-Fos, NFATc1, AKT, p38, c-Jun N-terminal kinase(JNK). Further, YBT extract suppressed degradation of$ I-{\kappa}B$. And ALR extract significantly restored the bone erosion by LPS treatment in mice. Conclusions : YBT extract showed more remarkable inhibition on osteoclast differentiation than CPC and ALR extracts in vitro. ALR extract showed remarkable inhibition on bone resorption in vivo. Thus, YBT extract can be a useful treatment for bone-loss diseases such as osteoporosis.