• 대한의료기공학회지
• /
• 제16권1호
• /
• pp.78-100
• /
• 2016

Objects : The purpose of this study is to value measuring of sagital, horizontal, frontal aspects of DITI as scale of low back pain. Methods : We investigated 16 patients with low back pain who had visited in the H Korean medicine hospital in Gimpo city, Gyeonggi Province from January 1st to, August 31th, 2016. We evaluated the change of them by using Digital Infrared Thermographic Imaging. Results : There is meaningful differences in values for CV12 in pretest and posttest which were observed by DITI. In the Correlation analysis of values of meridian points in pretest and posttest, there is relation ΔSagital04(ST25/BL52), ΔSagital04(CV4/GV3) with low back pain. Conclusions : These results suggest that ΔSagital04(ST25/BL52), ΔSagital04(CV4/GV3) can be used as value of Low back pain.

• Reproductive and Developmental Biology
• /
• 제32권2호
• /
• pp.71-79
• /
• 2008

Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권12호
• /
• pp.1695-1701
• /
• 2002

The purposes of this study were to optimize the in vitro maturation (IVM) and culture (IVC) systems of rabbit oocytes. Cytoskeletal structures in the germinal vesicle stage (GV) and during IVM are also investigated. Ovaries were transported from local slaughterhouses and the cumulus-oocyte complexes (COCs) were collected from ovarian follicles (${\geq}1mm$). COCs were randomly allocated to TCM199-based medium ($T_1$, TCM-199) supplemented with $NaHCO_3$, glucose, sodium pyruvate and FSH ($T_2$), $T_2+E_2+LH$ ($T_3$), $T_3+FBS$ ($T_4$), or $T_1+E_2+LH+FSH+FBS$ ($T_5$), for IVM. In Experiment 1, COCs were retrieved from the follicles and 51 GV oocytes were fixed in the fixative (MTSB-XF) for nuclear and cytoplasmic examinations. In Experiment 2, progressive changes of both the nucleus and the cytoskeleton were examined at 0, 6, 16, and 20 h after IVM. Maturation (MR) and developmental rates were assessed in Experiment 3. Cytoplasmic microtubules (MT) were clearly observed in rabbit GV oocytes. To our knowledge, this is the first report that describes the appearance of MT structures in the GV stage ooplasm. Tremendous variations in cytoskeletal alterations were observed among treatments with the exception of the vitelline ring (VR), which is constantly visible and unchanged during maturation. Germinal vesicle breakdown (GVBD) does not occur at 6 h after onset of maturation culture. When the oocytes for IVM were collected within 2 h, results from Experiment 3 showed that rates of nuclear maturation were 42, 8, 42, 37 and 65% at 16 h of IVM for $T_1$ through $T_5$, respectively, in which $T_1$, $T_4$ and $T_5$ had significantly greater MR than those in other groups (p<0.05). Morula/blastocyst development after parthenogenetic activation ranged from 20 to 63% with significantly greater rates in $T_3$, $T_4$ and $T_5$ (p<0.05). These results suggested that oocytes recovered from slaughterhouse ovaries can be matured and parthenogenetically activated in vitro, but the MR remained low in this study. Addition of $E_2$ and LH in the medium may be beneficial for cytoplasmic maturation, but FBS exerts a nega- tive role in the subsequent development of parthenogenetic embryos when energy substrates are provided in the IVC media. More studies are required for improving the MR and further development of the GV stage rabbit oocytes.

• 대한한의학회지
• /
• 제22권3호
• /
• pp.119-128
• /
• 2001

Objectives: Daechu (Dazhui: GV 14 (Governor Vessel))-point is located between the spinous process of the 7th cervical vertebra and that of the 1st thoracic vertebra. GV 14 has been used to treat high fever, neck pain, common cold, headache and so on. Fever may badly affect the improvement of stroke patients, so we investigated whether wet-cupping at GV 14 had effects on fever. Methods: In this study, 100 stroke patients were studied from Nov. 1999 to Oct. 2000. They were divided into the Sample group (n=49) and Control group (n=5l). The Sample group (n=49) was divided into Sample-Severe (n=2l), Sample-Mild (n=12), and Sample-Normal groups (n=16) and the Control group (n=5l) was divided into Control-Severe (n=8) and Control-Mild (n=43). We checked body temperature 6 times (just before treatment, after 30 ruin., 60, 90, 120 (2 hrs.), and 240 ruin. (4 hrs.)) in the Sample group and 3 times (just before treatment, after 120 min. (2 hrs.), and 240 ruin. (4 hrs.)) in the Control group. Results: In comparison with fever between before treatment and after 2 and 4 hours in each group, fever in the Sample subgroups decreased significantly in all cases, fever in the Control subgroups didn't decrease significantly in most cases except fever after 4 hours in the Control-Mild group. In comparison with fever differences between the Sample and Control group, fever of the Sample group more significantly decreased than that of the Control group in all comparisons. In comparison with fever among sample subgroups, fever of the Sample-Severe group decreased more than that of the Sample-Mild group but it was not significant. Conclusions: This study suggested that wet-cupping at GV 14 has significant effects on fever in stroke patients. We hope that this treatment will be used more widely as an emergent treatment.

• 한국한의학연구원논문집
• /
• 제16권1호
• /
• pp.43-50
• /
• 2010

Background : The varieties of manifestations referred to headache and vertigo have been long complicated physicians in the efficient diagnosis and treatment of these diseases. Objectives : Establish a distinctive and efficient acupuncture method for the treatment of headache and dizziness based in literature research. Method : We reviewed four Korean medical literature, "治腫指南Guide to Swollen Sore Treatment", "東醫寶鑑Treasured Mirror of Eastern Medicine", "鍼灸經驗方Experiential Prescriptions of Acupuncture and Moxibustion", and "舍岩鍼法Essential Rhymes on Acupuncture and Moxibustion by Master Sa-am", and analyzed the therapeutic characteristics in the treatment of headache and vertigo. Result : 1. "治腫指南Guide to Swollen Sore Treatment" inserted needle in GV20百會 and waited until the skin erupts and heat burns, then applied in those regions affected. 2. "東醫寶鑑Treasured Mirror of Eastern Medicine" mostly applied moxibustion on headache, however, needling acupoints such as GV20百會, GV24神庭, GV23上星, GV22顖會 were also considered important. 3. "鍼灸經驗方Experiential Prescriptions of Acupuncture and Moxibustion" used moxa cones on headache in order to disperse heat and direct Gi downward. 4. "校勘 舍岩道人鍼法Essential Rhymes on Acupuncture and Moxibustion by Master Sa-am" defined the cause of dizziness, wood excavates earth and earth kills water. Conclusions : After previous study on eye, oral, glottal, labial, dental diseases and stroke, we could also find various efficient methods according to headache and vertigo, through literature research of Korean medical classics. This study will concurrently result in establishing distinctive therapeutic method characteristic of Korea.

• Korean Journal of Acupuncture
• /
• 제38권4호
• /
• pp.197-208
• /
• 2021

Objectives : The aim of this study was to conduct a scoping review focusing on acupuncture points information applied in experimental studies using electroacupuncture for stroke. Methods : First, we set the research question of this study to identify which acupuncture points were studied for various symptoms of post-stroke sequelae in clinical and animal experiments. For this purpose, among the records searched through EMBASE, experimental studies including acupuncture points used in electroacupuncture treatment for stroke were selected as inclusion criteria for this study. The acupoints information used in each experiment was extracted from the included studies according to the type of study design and symptoms of stroke sequelae, and quantitative analysis was performed. Results : A total of 973 studies using acupuncture treatment, of which 429 papers including electroacupuncture were analyzed. Most of the animal experiments were conducted on general conditions instead of specific symptoms of stroke, and in clinical studies, electroacupuncture studies were conducted on symptoms such as hemiplegia and spasticity, cognitive and mental problems, dysphagia, shoulder problems, and depression that occur after stroke. Acupuncture points such as GV20, ST36, LI11, and LI4 were mainly used regardless of stroke symptoms. Except for these acupuncture points, GV26, GV14, GV16, and GV24 were frequently used in animal experiments, and TE5, LI15, LI10, and LR3 were widely used in clinical studies. Conclusions : Although this study extracted and analyzed only the frequent acupuncture points in the electroacupuncture study for stroke. However, in the future, it is necessary to be able to secure the evidence for acupuncture treatment elements through comparative studies between different acupuncture points or other elements constituting electroacupuncture.

• Korean Journal of Acupuncture
• /
• 제21권4호
• /
• pp.167-177
• /
• 2004

Objective : The purpose of this study is as follows. We classify headache into ten kinds as mentioned in DongUiBoGam(東醫寶鑑), and are going to present each acupuncture & moxibustion treatment. Methods : For the purpose of looking for each treatment, we referred to a. large number of literature for headache treatment from ancient to malearn. Results : The ten kinds of headache have each treatment. above all, In treatment of headache due to middle - headache (正頭痛) and megrim(偏頭痛) have pain in the formable of ache part. Therefore, general point such as GV20, GV23, ST8, TE23, Extra Meridian are used in turn, also wind-cold-headache(風寒頭痛) and many kind of headache such as GV16, LI4, GB20 are mainly used much in turn. Especially BL is in common use. Conclusions : We conclude that ten kinds of lumbago have each different acupuncture & moxibustion points and treatment, so if we follow each treatment we might obtain more higher rate the treatment of headache.

• 한방안이비인후피부과학회지
• /
• 제11권1호
• /
• pp.162-179
• /
• 1998

In the literatual studies on the yupung(油風), the results were as follows. 1. Yupung(油風) was called 'Quijidu(鬼지頭)', 'Quichedu(鬼剃頭)', 'Ballak(髮落)' etc. It was a localized loss of hair in round or oval ares without any subjective symptom 2. The etiology and pathogenesis of Yupung were disorder of vital energy& blood circulation caused by deficiency of blood and wind-dryness syndrome, energy-stagnation and blood stasis, impairment of the liver & kidney. The treatments of Yupung were invigorating the liver & kidney, clearing away heat-evil and cooling blood, nourishing the liver & kidney, activating blood circulation to dissipate blood stasis etc, 3. In the frequency of prescription, the most numerous prescription is Shineuingyangjindan(神應養眞丹) and the next are Tonggyuhwalhyultang(通竅活血湯) & Chilbomiyumdan(七寶美髥丹), 4. In the frequency of medicine, the most numerous medicine is Radix Angelicae Sinensis(當歸) and the next are Rhizoma Rehmanniae Praeparatae(熟地黃) & Rhizoma Ligusiici Chuanxiong(川芎). 5. In classification of drug action, medicines of clearing away wind-heat evil and invigorating yin used to be very busy. 6. In classification of four characters, the most parts are warm medicine. 7. In classification of five tastes, the most numerous tastes are sweet and bitter tastes. 8. In classification of toxicity, the most is non-toxic medicines. 9. In classification of the channel distribution, the most is the medicine that belongs to liver channel. 10. In acupuncture theraphy, it was used 'GV20(百會)', 'G20(風池)', 'GV16(風府)', 'GV14(大椎)', 'LI4(合谷)' etc. in body acupuncture, was used 'S25(天樞)', 'GV14(大椎)', 'B13(肺兪)', 'LI11(曲池)' etc. in moxibustion.

• 대한한방내과학회지
• /
• 제37권5호
• /
• pp.831-838
• /
• 2016

Objective: To describe the effectiveness of traditional Korean medicine (TKM) on a patient showing aggressive behavior after a traumatic brain injury. Methods: We used complex TKM treatments. The acupoints used were as follows: Soyangin Taegeuk acupuncture (HT3, KI3, and SP3) and acupoints frequently used in hemiparesis (GV20, GV24, ST4, LI11, LI4, ST36, GB34, SP9, and LR3). Moxibustion was also applied to GV20, and herbal medicine (Samulanshin-tang-gami, 四物安神湯 加味方) was administered. The efficacy was evaluated at baseline, week 8, and week 16 using the Modified Overt Aggression Scale (MOAS). Results: After 8 weeks, the total MOAS score was decreased from 13 to 5, and this score was maintained until week 16. The average sleep time was increased and waking up during the night was decreased. Conclusions: We suggest that TKM could be effective for reducing aggressive behavior and alleviating sleep disturbances. Further studies are needed.

• 한국임상수의학회지
• /
• 제23권1호
• /
• pp.65-68
• /
• 2006

개 추간판 디스크의 2증례를 수침치료를 하였다. 증례 1은 경부 디스크(C3-C4)의 증례이었으며, 1-2차 치료에서는 덱사메타손(1 mg/ml), 3-5차 치료에서는 염산치아민(25mg/ml)을 각각 수침하였다. 사용혈위는 GV-16, GB-20, BL-10, LU-7, LI-4 및 SI-6이었다. 2-5차 치료에는 GV-6, GV-20 및 SP-6의 혈위를 추가하였다. 3-5차 치료에는 2% 염산리도카인(0.2ml)를 이용하여 견갑아래근 및 상완세갈래근의 압통점에 각각 수침하였다. 5차 치료 후 마비증상은 소실되어 치료를 중단하였으며, 5개월 후 증상의 재발은 인정되지 않았다. 증례 2는 요부디스크의 증례(L1-L2)로 1-2차 치료에는 덱사메타손 수침을 3-4차치료에는 염산치아민을 각각 수침하였다. 사용혈위는 GV-6를 주혈로, ST-36, ST-40, GB-34, ST-41 및 BL-40을 부혈로 각각 사용하였다. 또한 허리장골 늑골근 및 대퇴네갈래근의 압통점에 2% 염산리도카인(0.2ml)를 각각 수침하였다. 4차 치료 후 마비증상이 호전되어 치료를 중단하였고, 5개월 후 현재 까지 증상의 재발은 인정되지 않았다. 이상의 증례를 통하여, 덱사메타손과 염산치아민의 수침 및 리도카인을 이용한 압통점치료의 병용은 개의 경부 및 요부디스크의 증상을 효과적으로 콘트롤 할 수 있는 것으로 판단되었다.