• Restorative Dentistry and Endodontics
• /
• v.31 no.6
• /
• pp.460-469
• /
• 2006

This article complies a survey on the replacement of the posterior restorations and accesses possible factors that influence the replacement of posterior restorations. The data was collected from patients that visited department of conservative dentistry from Dec 1st 2003, to Sep 3rd 2004. Teeth was restricted to posterior permanent teeth. 9 dentists recorded age, gender of patients, tooth location, cavity farm and restorative material. They rated marginal adaptation, anatomic form, secondary caries of old restoration by modified Ryge criteria system. The statistical analysis was performed with Chi square test (p < 0.05) for replacement ratio according to patients, tooth factor and One way ANOVA was performed for comparison of old restoration according to restorative material. The results were as follows; 1. The female (62%) was statistically higher ratio than the male (38%). 2. The distribution of replacement case according to age, the rate of replacement was in descending order, 20's (38.3%), 40's (16.8%), 30's (15.9%), 10's (11.1%), 50's (9.2%), 60's (8.7%). 3. The rate of replacement was 88% for molar and 12% for premolar (p $gt; 0.05). 4. The rate of replacement was 39% for maxillar and 61% for mandible (p $gt; 0.05). 5. The material of restorations was amalgam (69%), gold inlay (17%), composite resin (13%). 6. In rating system by modified Ryge criteria system on margin adaptation, there was statistically significant difference between amalgam and gold inlay. But on anatomic form and caries, there was no statistically significant difference among the material of restorations.

• Restorative Dentistry and Endodontics
• /
• v.30 no.1
• /
• pp.31-37
• /
• 2005

The aim of this study was to evaluate the effectiveness of sealer placement in simulated root canal extensions. Forty resin blocks were attained from the Endo-training Bloc. In each block. The simulated root canal was made with $\#20$, 80taper GT file. After each block was longitudinally split into two halves, a standardized groove was prepared on one canal wall of two halves to simulate the canal extensions with various irregularities. The two halves of each block were assembled and all simulated root canals were obturated by single cone method with AH26 sealer. Four different methods of sealer placement were used: group A, $\#20$ K-file; group B, ultrasonic file; group C, lentulo spiral; group D, EZ-Fill bi-directional spiral. All obturated blocks were stored in $100\%$ humidity at $37^{\circ}C$ for 1 week, Using a low speed saw, each block was sectioned horizontally. Images of the sections were taken using a stereomicroscope at $\times$ 30 magnification and a digital camera. The amount of the sealer in the groove was evaluated using a scoring system, a higher score indicated better sealing effectiveness. The data was statistically analysed by Fisher's Exact Test. The sealing score was the lowest, specially at the middle area of canal extensions in group A, and that was statistically significant difference from other groups. In conclusion, the ultrasonic file, lentulo spiral and EZ-Fill bi-directional spiral were effective methods of sealer placement in simulated canal extensions. The K file was the least effective method, specially at the middle area of canal extensions.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.66 no.4
• /
• pp.365-374
• /
• 2021

Soybean isoflavones are essential secondary metabolites synthesized through the phenylpropanoid pathway, and they play vital roles in human health. Isoflavone content is a complex quantitative trait controlled by multiple genes, and the genetic mechanisms underlying isoflavone biosynthesis remain largely unknown. Therefore, the present study analyzed the content of isoflavone and expression of six key genes involved in its biosynthesis (i.e., CHS6, HID, IF7GT, IF7MaT, GmIMaT1, and GmIMaT3) during soybean seed germination. Isoflavone content was quantified using high-performance liquid chromatography, and isoflavone biosynthetic gene expression was analyzed using quantitative real-time PCR. Two cultivars, namely 'Daepung2ho' and 'Pungsannamulkong', which are high- and low-isoflavone cultivars, respectively, were used. Isoflavone accumulation gradually increased with the progression of the germination period. As such, malonyl glucosides accounted for over 80% of the total content, whereas acetyl glucosides were present at trace amounts. Transcriptional analysis of isoflavone biosynthetic genes demonstrated expression patterns parallel to isoflavone content; however, there was no clear correlation between isoflavone content and gene expression. Moreover, most isoflavone biosynthetic genes showed different expression patterns depending on the individual gene or genotypes. Among the tested genes, HID showed consistently higher expression, except at 3 days after germination, and its expression was upregulated in 'Daepung2ho' but downregulated in 'Pungsannamulkong'. In addition, all tested genes exhibited different expression patterns between cotyledons and hypocotyls and responded differently to the germination period. These findings suggest that the expression levels of isoflavone biosynthetic genes are not consistent with the germination period and appear to be genotype-dependent.

• Biomedical Science Letters
• /
• v.7 no.1
• /
• pp.1-5
• /
• 2001

A cDNA of coagulation Factor IX gene has been screened from the $\lambda$gt11 human fetal liver cDNA library, and used to construct a 2.8-kb full length cDNA after recombining with the N-terminal fragment from pTZ-FIX. Human genomic DNA was isolated, digested with the restriction endonucleases, TaqI, EcoRI, and HindIII, and Southern hybridization was performed using the full length factor IX cDNA as a probe. The hybridized bands generated by the restriction endonucleases were the followings: TaqI, 0.3, 1.0, 1.6, 1.8, 2.7, 3.7, and 5.3 kb bands; EcoRI, 1.8, 4.8, 4.9, 5.5, 6.8, and 12.6 kb bands; HindIII, 4.1, 4.4, 5.2, 5.8, 7.6, and 12.5 kb bands. When the Southern bands were physically mapped along the genome, about 50-kb continuous region harboring almost all of the genomic region of Factor Ⅸ gene was covered. These results suggest a possibility of using an exonal cDNA probe to diagnose abnormalities including large deletions, insertions, and rearrangements along the genome, if there is any.

• The Korean Journal of Pharmacology
• /
• v.30 no.3
• /
• pp.273-289
• /
• 1994

Reversible brain ischemia was produced by occluding both common carotid arteries for 5 min, and the effects of aminoguanidine (AG), $DL-{\alpha}-difluoromethylornithine$ (DFMO), MK-801, and nimodipine (NM) on the ischemia induced changes of the polyamine, glutamate and acetylcholine levels in the hippocampus CA1 subfield and the specific $[^3H]\;MK-801$ binding to the hippocampus synaptosomal membranes were studied with a histological reference of the cresyl violet stained hippocampus. The basal putrescine level $(PT:\;74.4{\pm}8.8\;nM)$ showed a rapid increase (up to 1.7 fold) for 5 min of ischemia, remained significantly increased for 6 h, and then resumed the further increase to amount gradually up to about 3 fold 96 h after recirculation. However, the level of spermidine was little changed, and the spermine level showed a transient increase during ischemia followed by a sustained decrease to about 40% of the preischemic level after recirculation. The increase of PT level induced by brain ischemia was enhanced with AG or MK-801, but it was reduced by DFMO or NM. The basal glutamate level $(GT:\;0.90{\pm}0.l4\;{\mu}M)$ rapidly increased to a peak level of $8.19{\pm}1.14\;{\mu}M$ within 5 min after onset of the ischemia and then decreased to the preischemic level in about 25 min after recirculation. And NM reduced the ischemia induced increase of GT level by about 25%, but AG, DFMO and MK-801 did not affect the GT increase. The basal acetylcholine level $(ACh:\;118.0{\pm}10.5\;{\mu}M)$ did little change during/after brain ischemia and was little affected by AG or NM. But DFMO and MK-801, respectively, produced the moderate decrease of ACh level. The specific $[^3H]\;MK-801$ binding to the hippocampus synaptosomal membrane was little affected by brain ischemia for 5 min. The control value (78.9 fmole/mg protein) was moderately decreased by AG and MK-801, respectively but was little changed by DFMO or NM. The microscopic findings of the brains extirpated on day 7 after ischemia showed severe neuronal damage of the hippocampus, particularly CA1 subfield. NM and AG moderately attenuated the delayed neuronal damage, and DFMO, on the contrary, aggravated the ischemia induced damage. However, MK-801 did not protect the hippocampus from ischemic damage. These results suggest that unlike to the mode of anti-ischemic action of NM, AG might protect the hippocampus from ischemic injury as being negatively regulatory on the N-methyl-D-aspartate (NMDA) receptor function in the hippocampus.

• Journal of Food Hygiene and Safety
• /
• v.10 no.1
• /
• pp.45-51
• /
• 1995

Ozone treatment was applied to ginseng powder for the improving hygienic quality of ginseng powder. A bacterial strain was isolated form ginseng powder contaminated. The strain designated as GT4, was identified as Escherichia coli species by IMVIC test method. Ozone inhibited strongly total bacteria and coliforms in ginseng powder(initial concentration 103/g) at 0.35 ppm. High ozone concentration reduced death time of the total bacteria in ginseng powder. However, ozone treatment caused significant degradation in saponins of ginseng powder. Ozone treatment also caused a increase in Hunter's color L value and decrease in a and b values of ginseng powder.

• 한국신재생에너지학회:학술대회논문집
• /
• 2008.05a
• /
• pp.239-242
• /
• 2008

As the distributed generation becomes more reliable and economically feasible, it is expected that a higher application of the distributed generation units would be interconnected to the existing grids. In this context, the Micro Gas Turbines (MGT) by using Bio-gas is being considered as a promising solution. In order to propose a feasible concept of those technologies such as improving environmental effect and economics, we performed a sensitivity study for a biomass fueled MGT using a simulation model. The study consists of 1) the fundamental modeling using manufacturer's technical specifications, 2) the correction with the experimental data, and 3) the prediction of off-design characteristics. The performance analysis model was developed by PEPSE-GT 72, commercial steam/gas turbine simulation technicque.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.24 no.8
• /
• pp.600-605
• /
• 2014

Two mufflers for a large-size sedan are suggested aiming (1) sporty-sound and (2) quiet-sound as well as both satisfying low back-pressure and low manufacturing cost. Transmission loss prediction considering heat and flow may increase the accuracy and reduce the development cost in muffler design; thus, GT-power prediction considering heat, flow, and acoustics is utilized. By understanding the fundamentals of flow-acoustic theory in small orifice(hole), an effective muffler design concept is proposed. Vehicle tests show the consistence with predictions for sound; also a back-pressure test bench confirms the advantage in pressure drop for both suggested mufflers. Those suggested mufflers also have advantages in manufacturing cost due to simplicity of the design.

• Journal of Energy Engineering
• /
• v.15 no.1 s.45
• /
• pp.67-73
• /
• 2006

A calculation method on performance impact of the controllable operations parameter such as GT inlet filter ${\Delta}P$, compressor efficiency, and condenser pressure was devised to achieve best performance of combined cycle power plant with the on-line performance monitoring system. This method calculates the performance impact on the deviation between 'Expected' values and 'Actual' values. Controllable parameter targets are determined based on achievable performance given existing equipments and control conditions.

• The Microorganisms and Industry
• /
• v.14 no.1
• /
• pp.17-20
• /
• 1988

A genetic analysis of the complex Bacillus subtilis transcriptional apparatus is essential to understand the function, regulation, and interaction of the transcriptase components during growth and sporulation. This approach in Escherichia coli has uncovered fundamental mechanisms regulating gene expression Cole and Nomura, 1986; Lindahl and Zengel, 1986) and an analysis of the B. subtilis transcriptase will allow comoparison of the E.coli system to another bacterium that has evolved under different selective pressures. To this end we used antibody probes to isolate the alpha, beta, and beta' core subunit genes from a .lambda.gtill expression vector library. To address the question of function ans regulation of the minor sigma factors that confer promoter specifity on the polymerase core (Losick et al., 1986), we used the same approach to isolate the gene for the 37,000 dalton sigma factor, sigma-37.