• Journal of Plant Biotechnology
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• 제7권4호
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• pp.233-240
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• 2005

Slices of embryonic axis of mature pea (Pisum sativum L. cv. Green Arrow) seeds were used as explant. Transformation of explants was done via Agrobacterium tumefaciens bearing vector pBI-P5CS construct. The best results for inoculation of explants were obtained when they were immersed for 90 s at a concentration of $6{\times}10^8$ cell $ml^(-1)$ of bacterial suspension. Transformed pea plants were selected on $50\;mg\;l^(-1)$ kanamycin and successful transformants were confirmed by PCR and blotting. Transgenic plants were further analyzed with RT-PCR to confirm the expression of P5CS. Transgenic plants and non-transgenic plants were treated with different concentrations of NaCl 0 (control), 100, 150 and 200 mM in culture medium. Measurement of proline content indicated that transgenic plants produced more amino acid proline in response to salt in comparison with non-transgenic plants. Photosynthetic efficiency in transgenic plants under salt-stress was more than that of non-transgenic plants.

• BMB Reports
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• 제41권6호
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• pp.472-478
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• 2008

Three Arabidopsis cDNAs, MAM1, CYP79F1, and CYP83A1, required for aliphatic glucosinolate biosynthesis were introduced into Chinese cabbage by Agrobacterium tumefaciens-mediated transformation. The transgenic lines overexpressing MAM1 or CYP83A1 showed wild-type phenotypes. However, all the lines overexpressing CYP79F1 displayed phenotypes different from wild type with respect to the stem thickness as well as leaf width and shape. Glucosinolate contents of the transgenic plants were compared with those of wild type. In the MAM1 line M1-1, accumulation of aliphatic glucosinolates gluconapin and glucobrassicanapin significantly increased. In the CYP83A1 line A1-1, all the aliphatic glucosinolate levels were increased, and the levels of gluconapin and glucobrassicanapin were elevated by 4.5 and 2 fold, respectively. The three CYP79F1 transgenic lines exhibited dissimilar glucosinolate profiles. The F1-1 line accumulated higher levels of gluconapoleiferin, glucobrassicin, and 4-methoxy glucobrassicin. However, F1-2 and F1-3 lines demonstrated a decrease in the levels of gluconapin and glucobrassicanapin and an increased level of 4-hydroxy glucobrassicin.

• 원예과학기술지
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• 제28권6호
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• pp.964-972
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• 2010

피트모스+버미큘라이트(5:5, A), 피트모스+펄라이트(7:3, B), 코코피트+펄라이트(7:3, C), 코코피트+피트모스+펄라이트(3.5:3.5:3.0, D), 왕겨+코코피트+펄라이트(2:7:1, E), 그리고 왕겨+코코피트(3:7, F)의 6종류 상토를 혼합하여 상부 직경 10cm의 플라스틱 포트에 충전한 후 '설향' 딸기의 모주에서 발생한 런너를 고정시켜 번식시키면서 상토 물리 화학성이 자묘 생육에 미치는 영향을 구명하기 위하여 본 연구를 수행하였다. 자묘 육묘용 상토의 용기용수량과 기상률은 상토별 차이가 뚜렷하였으며 E와 F 상토는 용기용수량이 낮고 기상률이 높아 상토의 수분관리에 어려움이 있을 것으로 판단하였다. 피트모스가 혼합된 상토 A, B, 및 D의 질소농도가 높았고, 왕겨를 혼합한 상토 E와 F의 질소 및 인산 농도가 낮았다. 또한 코코피트가 혼합된 상토가 피트모스가 혼합된 상토 보다 K 농도가 월등히 높았다. A상토에서 '설향' 자묘를 재배한 결과 약 13mm에서 관부직경의 회귀선이 형성되어 가장 굵었고, F, B와 C 상토에서 육묘한 자묘도 관부 직경의 회귀선이 약 10mm 이상에서 형성되고 있어 자묘의 생육에 바람직하다고 판단하였다. '설향' 자묘의 생체중은 A 상토에서 육묘한 경우 식물체당 10g 후반에서 회귀선이 형성되었지만, C, F, D, E, 그리고 B 상토의 순으로 가벼워졌다. '설향' 자묘의 건물중도 생체중과 유사한 경향을 보였으며 A, C 및 F 상토에서 비교적 건물중 생산량이 많았고, B 상토에서 적었다. 생체중과 건물중 생산량이 많을 경우 보편적으로 식물이 건전한 생육을 하고 있음을 의미하며, 이러한 판단을 적용할 때 A, C 및 F 상토가 자묘생육을 위해 바람직하다고 판단하였다.

• 한국작물학회지
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• 제33권4호
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• pp.386-391
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• 1988

보리의 교배육종에서 교배조합의 양부를 초기세대에 검정할 수 있는가를 Sakai의 이론에 따라 구명하기 위하여 보리의 수량형질인 추수, 입수, 수중, 개체별 입중에 대해서 5개 교배조합을 가지고 조사를 실시한 결과를 요약하면 다음과 같다. 1. 입수의 경우 3개 조합에서 그리고 수중의 경우는 1개 조합에서 상가적유전분산이 부의 방향으로 계산되었고 교배조합의 우량도를 계산할 수 없었으나 나머지 조합과 형질에서는 무난히 계산이 가능하였다. 2. 수수에 대한, 교배조합의 우량도 Y는 SB 76588$\times$SB72648 조합이 제1위였다. 또 이 조합은 F$_4$ 집단의 상위 5 % 및 10%내 식물의 평균순위에서도 2위를 하여 수수에 대해서는 이 조합이 5개 조합중 가장 좋은 조합으로 인정되었다. 3. 수당입수에 있어서는 3개 조합에서 Y값의 추정이 불가능하였으나 나머지 2조합을 가지고 보았을 때 F$_4$ 집단의 순위가 높고 Y값도 높은 밀양 006$\times$수원20003 조합이 우량조합인 것으로 보이었다. 4. 수종에서는 Y계산이 불가했던 1개 조합을 제외한 나머지 조합에서 Y에 의한 순위와 F$_4$에의 한 순위와 대체로 일치하였고 밀양006$\times$수원 20003 조합이 우수조합으로 나타났다. 5. 개체별 입중에서도 Y값에 의한 순위와 F$_4$에 의한 순위가 대체로 일치하였고 SB76588$\times$SB72648 조합이 가장 우수한 조합인 것으로 나타났다.

• 한국가스학회지
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• 제15권1호
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• pp.74-80
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• 2011

최근 국내의 많은 에너지 플랜트가 10여년 이상 가동되면서 설비의 장수명화 및 노후설비에 대한 건전성 확보에 대한 요구가 증가하고 있다. 이에 따라 플랜트의 RAM (reliability, availability and maintainability)에 대한 중요도가 높아지고 있다. 플랜트의 RAM을 높이기 위해 RBI (Risk Based Inspection)는 중요한 핵심 기술이다. RBI 기술은 주로 정류 플랜트 설비, 매설배관 설비, 원자력플랜트 설비 등의 에너지 플랜트 분야에서 개발이 진행되어 왔다. 하지만, 기 개발된 RBI 절차는 화력 발전플랜트 등 다른 에너지 플랜트에서의 적용이 어려웠다. 본 연구에서는 에너지 플랜트에서의 최적 예방점검을 위한 일반적인 RBI 표준 절차를 개발하여 보고하였다.

• 한국유기농업학회지
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• 제12권1호
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• pp.101-114
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• 2004

This study was carried out to clarify the effects of antagonistic microorganism, Bacillus sp. JC181 isolated from the greenhouse soil grown cucumber plants on the growth inhibition of plant pathogen, fusarium wilt (Fusarium oxysporum) occurred in cucumber plants in greenhouse. Antagonistic bacterial strains were isolated and were investigated into the antifungal activity of the antagonistic microorganism against fusarium wilt. Screened fourteen bacterial strains which strongly inhibited F. oxysporum were isolated from thc greenhouse soil grown cucumber plants, and the best antagonistic bacterial strain designated as JC181, was finally selected. Antagonistic bacterial strain JC181 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. JC181 showed 58.2% of antifungal activity against the plant pathogen growth of F. oxysporum. By the bacterialization of culture broth and heated filtrates of culture broth, Bacterial strain, Bacillus sp. JC181. showed 91.2% and 260% of antifungal activity against F. oxysporum, respectivrly.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.287-287
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• 2022

In this study, we analyze RNA-seq data from OxF3Hand WT at several points (Oh, 3 h, 12 h, and 24 h) after WBPH infection. A number of the genes were further validated by RT-qPCR. Results revealed that highest number of DEGs (4,735) between the two genotypes detected after 24 h of infection. Interestingly, many of the DEGs between the WT and OsF3H under control conditions were also found to be differentially expressed in OsF3H in response to WBPH infestation. These results indicate that significant differences in gene expression between the "OxF3H" and "WT" exist as the infection time increases. Many of these DEGs were related to oxidoreductase activity, response to stress, salicylic acid biosynthesis, metabolic process, defense response to pathogen, cellular response to toxic substance, and regulation of hormones level. Moreover, genes involved in salicylic acid (SA) and Ethylene (Et) biosynthesis were upregulated in OxF3H plants while jasmonic acid (JA), Brassinosteroid (Br), and abscisic acid (ABA) signaling pathways were found downregulated in OxF3H plant during WBPH infestation. Interestingly, many DEGs related to pathogenesis such as OsPR1, OsPR1b, NPR1, OsNPR3 and OsNPR5 were found significantly upregulated in OxF3H plants. Additionally, genes related to MAPKs pathway, and about 30 WRKY genes involved in different pathways were found upregulated in OxF3H plants after WBPH infestation. This suggests that overexpression of the OxF3H gene leads to multiple transcriptomic changes and impact plant hormones, pathogenic related and secondary metabolites related genes and enhancing the plant resistance to WBPH infestation.

• Molecules and Cells
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• 제27권3호
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• pp.329-336
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• 2009

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.367.1-367.1
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• 2002

Flavo.no.ids are a diverse group of phytqchemicals that are produced by various plants in high quantities. Dietary flavonoids in edible plants can be further subdivided into. several structural groups. The large number of compounds arises from various combinations of multiple hydroxyl and methoxyl groups substituting the basic flavonoid skeleton. The chemopreentive activity of flavonoids is dependent on their structural features. (omitted)

• 한국응용곤충학회지
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• 제34권1호
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• pp.1-8
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• 1995

The relationships between aphids, plants, other organisms and some physical components of the environment are reviewed. Aspects considered include year cycles, polymorphism fecundity, relationship of different groups of aphids with particular groups of plants, honeydew, alarm pheromones, aposematic colouring, camouflage, colour variation within species, morphological variation within species, multivariate analysis and problems of its interpretation, parasitism, stridulating mechanisms, predators, coevolution of plants and aphids, plant galls, trapping aphids and the interpretation of trap catches, an curation of aphid collections. References are given to sources of information about aphids, with special reference to the Korean fauna.