• The Korean Journal of Physiology and Pharmacology
• /
• v.3 no.5
• /
• pp.471-479
• /
• 1999

The Kv channel activity in vascular smooth muscle cell plays an important role in the regulation of membrane potential and blood vessel tone. It was postulated that increased blood vessel tone in hypertension was associated with alteration of Kv channel and membrane potential. Therefore, using whole cell mode of patch-clamp technique, the membrane potential and the 4-AP-sensitive Kv current in cerebral arterial smooth muscle cells were compared between normotensive rat and one-kidney, one-clip Goldblatt hypertensive rat (lK,lC-GBH rat). Cell capacitance of hypertensive rat was similar to that of normotensive rat. Cell capacitance of normotensive rat and 1K,lC-GBH rat were $20.8{\pm}2.3$ and $19.5{\pm}1.4$ pF, respectively. The resting membrane potentials measured in current clamp mode from normotensive rat and 1K,lC-GBH rat were $-45.9{\pm}1.7$ and $-38.5{\pm}1.6$ mV, respectively. 4-AP (5 mM) caused the resting membrane potential hypopolarize but charybdotoxin $(0.1\;{\mu}M)$ did not cause any change of membrane potential. Component of 4-AP-sensitive Kv current was smaller in 1K,lC-GBH rat than in normotensive rat. The voltage dependence of steady-state activation and inactivation of Kv channel determined by using double-pulse protocol showed no significant difference. These results suggest that 4-AP-sensitive Kv channels playa major role in the regulation of membrane potential in cerebral arterial smooth muscle cells and alterations of 4-AP-sensitive Kv channels would contribute to hypopolarization of membrane potential in 1K,lC-GBH rat.

• Korean Journal of Health Education and Promotion
• /
• v.23 no.1
• /
• pp.77-92
• /
• 2006

Objectives: This study is to investigate and to test the overall level of nutritional knowledge. attitude. practice and health status of the residents in rural area. Methods: The interview survey was performed in March 2004 with structured questionnaires to 510 respondents of the residents who lived in Muan-Gun of Jeonnam province. The questionnaire was the abridged Scale of the Nutritional Knowledge Attitude Practice(SNKA) and health status as the morbidity, subjective health status, health management, and health examination. The covariate, F-test or t-test, and Chi-squire method were used for some of the cross-sectional data. Results: Average weight of nutritional knowledge was $10.64{\pm}5.98$ by SNKA of the 20 items(0-20). Average weight of nutritional attitude practice was $64.59{\pm}7.72$ by SNKA of the 20 items(20-80). The level of the nutritional knowledge was lower than the level of the nutritional attitude practice. 77.3% of the respondents have been health management, 49.6% of the respondents have been health examination. A majority of the respondents(31.3%) had disease, while 13.5% of the respondents had chronic disease, and 17.8% of the respondents had acute disease. The level of nutritional knowledge and general characteristics was positively correlated while the relationships were positive between nutritional knowledge and health status, and between nutritional attitude practice and health status. Conclusion: These results suggested that education programs of the nutritional knowledge were necessary for the residents of rural area. And behavior change of the nutritional knowledge were necessary for the residents of rural area. Further research would be required to specify the necessities and operation researches.

• Journal of Pharmaceutical Investigation
• /
• v.37 no.1
• /
• pp.15-22
• /
• 2007

An oil-in-water solvent evaporation method was used to prepare the cyclosporin A (CyA)-loaded nanoparticles varying in poly (D,L-lactide-co-glycolide) (PLGA) polymer (RG 502H, RG 503H) and the amount of additive ethyl myristate (EM) or chitosan (CS). The particles were characterized for drug loading and entrapment efficiency by HPLC, surface morphology by scanning electron microscopy, particle size by dynamic light scattering and surface charge by Zetapotential. The results showed drug loadings ranging from 10.9% to 15.8% with high encapsulation efficiency (82.0-97.8%). SEM and DLS studies showed discrete and spherical particles with smooth surfaces and mean size ranging 257.6-721.7 nm. The additive EM or CS did not change the mean sizes of the nanoparticles, whereas by the coating effect of CS, the Zetapotential values of the CS-added nanoparticles were moved to the more positive direction as the amount of CS was increased. From the pharmacokinetic analysis, the nanoparticles formulations showed the higher bioavailability and MRT than $Neoral^{\circledR}$ While little adding effect of EM or CS was detected in pharmacokinetic profile when RG 503H was used as polymer carrier, more noticeable different pharmacokinetic behaviors could be observed in case of RC 502H. EM incorporation was found to elevate the $K_{el}$, whereas CS coating resulted in the decrease of F and $K_{el}$, which seems to be due to the function of CS as a barrier and a mucoadhesive coating.

• Preventive Nutrition and Food Science
• /
• v.4 no.2
• /
• pp.107-112
• /
• 1999

Effect of solvent extracts and juice supernatants from kimchis on the growth of various human cancer cells was studied, comparing with the actions on the normal cells. Inhibitory effect of kimchi extracts on[3H] thymidine incorporation n cancer cells was also investigated. The methanol extract, hexane extract and methanol soluble fraction (MSF) of 3-week fermented kimchi did not have growth inhibitory effect on Ac2F rat normal liver cells at the concentrations of 0.5~2%. However, marked decrease in the growth of AGS human gastric cancer cells was shown by the treatment of those extacts. The juice from the kimchi samples also suppressed the growth of K-562 human leukemia cells and MG-63 human osteosarcoma cells. Especially, the juice of 3-week fermented kimchi exhibited the strong growth inhibitory effect in MG-63 human osteosarcoma cells. At the photomicrographs, growth inhibition and morphological change of the cells treated with kimchi juice were observed. And the solvent extracts of 3-week fermented kimchi suppressed the growth of cancer morethan the extracts or juices from fresh and 6-week fermented kimchi. When AGS human gastric cancer cels were treated with the extracts of 3-week fermented kimchi, [3H] thymidine incorporation in the cells also decreased. These results showed that kimchi extracts and juices had growth inhibitory effects on human osteosarcoma, leukemia and gastric cancer cells, but had no toxicity to the normal cells. We suggest that kimchi might have anticancer effect in part due to inhibition of the growth and DNA synthesis of cancer cells.

• Journal of Korean Academy of Nursing
• /
• v.29 no.1
• /
• pp.48-60
• /
• 1999

This study was done to determine the factors influencing smoking-cessation behavior in female university students. A total of 534 students participated in this cross sectional study by answering a questionnaire. The data collection was done between September 1 and October 31, 1997 The measurement tools used in this study were the self help change process scale (Cronbach's alpha＝.9930 : developed by Oh & Kim, 1996) for smoking-cessation behaviors, the self efficacy scale(Cronbach's alpha＝.8250 : developed by Sherer et al, 1982), the sex role acceptance scale (KR－20＝.7757 : developed by Kim, 1991) and the social support scale(Cronbach's alpha＝.9172 : developed by Park, 1985). The summarized results are follows : 1. The mean scores for smoking-cessation behaviors in smokers (N＝150) was 91.72 that was considered a middle score compared to the total possible score of measurement tool (150.0). The mean score for smoking-cessation behaviors by smoking-cessation step showed significant different between the groups(F＝11.71, p＝.000). 2. The group with no experience in smoking(N＝332) showed a high general self efficacy score (t＝5.24, p＝.000), and more openness to sex role acceptance(t＝-2.15, p＝.032) compared to the group with smoking experience (N＝202). 3. General self efficacy, sex role acceptance, and social support were not different significantly between the groups according to the steps in smoking-cessation. 4. Significant factors influencing smoking-cessation behavior (total, sub concepts) were religion, sex role acceptance, social support, smoking duration, smoking attitude, time of smoking onset, amount of smoking, drinking, and perception of health status. 5. Smoking-cessation behaviors which explained 11% of the variance were smoking attitude, and smoking duration. In conclusion, this study identified factors influencing smoking-cessation behavior. Thereby it will help in the development of smoking-cessation intervention strategies. For future research, exploration other determinants of smoking cessation behaviors, evaluation of intervention efficiency, and comparative study by gender characteristics are needed.

• Journal of Ginseng Research
• /
• v.44 no.5
• /
• pp.738-746
• /
• 2020

Background: Red ginseng contains components, including microelements, vitamins, essential oils, and fatty acids, that can be used in skincare to delay the aging process. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis. Methods: Human dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using atomic force microscopy. Cytoskeletal changes and mRNA expression of biomarkers of aging, including that of procollagens I and VII, elastin, and fibrillin-1, were investigated. Collagen in a human skin equivalent treated with red ginseng was visualized via hematoxylin and eosin staining, scanning electron microscopy, and atomic force microscopy. Results and conclusion: The stiffness of fibroblasts was significantly reduced by treatment with red ginseng concentrations of ≥ 0.8 mg/mL. The ratio of F-actin to G-actin decreased after treatment, which corresponded to a change in fibroblast stiffness. The storage modulus (G') and loss modulus (G'') of the skin equivalent were both lowered by red ginseng treatment. This result indicates that the viscoelasticity of the skin equivalent can be restored by red ginseng treatment.

• Journal of the Semiconductor & Display Technology
• /
• v.7 no.1
• /
• pp.1-5
• /
• 2008

In this paper, we fabricated pressure difference type gas flow sensor using only dry etching technology by ICP-RIE(inductive coupled plasma reactive ion etching). The sensor's structure consists of a common shear stress type piezoresistive pressure sensor with an orifice fabricated in the middle of the sensor diaphragm. Generally, structure like diaphragm is fabricated by wet etching technology using TMAH, but we fabricated diaphragm by only dry etching using ICP-RIE. To equalize the thickness of diaphragm we applied insulator($SiO_2$) layer of SOI(Si/$SiO_2$/Si-sub) wafer as delay layer of dry etching. Size of fabricated diaphragm is $1000{\times}1000{\times}7\;{\mu}m^3$ and overall chip $3000{\times}3000{\times}7\;{\mu}m^3$. We measured the variation of output voltage toward the change of gas pressure to analyze characteristics of the fabricated sensor. Sensitivity of fabricated sensor was relatively high as about 1.5mV/V kPa at 1kPa full-scale. Nonlinearity was below 0.5%F.S. Over-pressure range of the fabricated sensor is 100kPa or more.

• Journal of Korean Academy of Nursing Administration
• /
• v.12 no.2
• /
• pp.225-232
• /
• 2006

Purpose: The purpose of this study was to analyze the services and cost of CVA patients in hospital-based home health care and compare the differences of home health care cost by hospital types. Methods: The subjects of this study were 5,756 home care patients with cerebrovascular disease. Data were collected by using home health care medical expense claims from 127 hospitals in 2004. Results: The home care service 'indewelling catheterization' was the highest(19.28%), and then 'nasogastric tube insertion and change(16.72%)', 'bladder irrigation(15.98)', 'wound management(simple dressing)(10.42%)' followed. Average home health care cost per visit was 39,943 won, and the highest 46,058 won in general hospitals and the lowest 33,922 won in tertiary hospitals, so there were statistically significant among the types of hospitals(F=1112.47, p<0.0001). Conclusions: The number of home health care patients has been rapidly growing with the increase of aged population and demand for home care services is rising. So, it could be urgent to develop a reasonable cost reimbursement system for home health services and to expend scopes of the roles of home care specialist nurses. Amid the demand of more detail understanding the present status of home care, our study can be contributed to provide fundamental information of home care in Korea.

• Journal of the Korean Institute of Landscape Architecture International Edition
• /
• no.1
• /
• pp.75-83
• /
• 2001

Residents of urban area like to be near rural animals during their daily life, so it is important conserve the suburban Yato landscape in Japan. This study targets the species of frogs that are commonly found in Yato paddies. It is necessary to various frogs inhabiting in Yato, because frogs are popular among Japanese, who as children enjoy capturing tadpoles and frogs. Its purpose is to clarify how that composition of frog species changes when the functionality of a frog habitat is diminished by urbanization. The survey, conducted in the Eastern Kanagawa area in central Japan, determined the distribution of each species of frog in grid cells measuring 0.5-$\textrm{km}^2$. It hsows wide distributing species (Hyla japonica; they always inhabit in all paddies), the middle range distributing species (Rhacophorus schlegelii and Rana porosa p.), the limited range distributing species (R. rugosa; they are most critical species, sine they are recognized only two cells), and so on. Correspondence analysis based on the frog species composition in each cell was performed to ascertain the adaptability of each species to various paddy field conditions. The results allowed us to classify cells into four groups according to the composition of the inhabiting species. And we recognized that the process by which frogs disappear occurs in reaction to either of two patterns of change. As paddy fields are improved by farmland consolidation, R. rugosa, R. ornativentris, R. japonica, and Bufo. japonica f. decline rapidly. In plateau areas, a smore andmore paddies are converted into strong, well-drained fields, only H. japonica and R. porosa p. remain. But in hilly areasd, the species composition becomes only H. japonica and R. schlegelii. Finally, we discuss the concept of ecological urban design in the context of the conservation of frog species in Yato paddies.

• Korean Journal of Dental Materials
• /
• v.43 no.1
• /
• pp.81-89
• /
• 2016

The purpose of this study was to evaluate the effect of the experimental and commercial fluoride varnishes on the Vickers hardness of bovine teeth. The experimental fluoride varnishes with 5 wt.% NaF were fabricated using Bis-GMA or rosin as the resin base. The components were mixed with over-head stirrer under warming up in a double boiler for 30 minutes. Four commercial fluoride varnishes (V-varnish, Flor-opal, Cavity shield, Fluor protector) were compared with the experimental fluoride varnishes. Vickers hardness was measured on the labial surface of bovine anterior teeth after applying fluoride varnish on 5th and 30th day. The surface of bovine teeth was observed with a scanning electron microscope before and after applying fluoride varnish and the change of the components on the bovine teeth surface was measured with EDX. In terms of hardness, the experimental rosin-based fluoride varnish showed significantly higher hardness on the 5th and 30th day than the control (bovine teeth without fluoride treatment)(p<0.05). EDX results showed that the fluoride content on the surface of bovine teeth treated with the experimental rosin-based fluoride varnish was highest on the 1st and 10th day. The higher hardness and fluoride content of experimental rosin-based fluoride varnish can be considered to be used as an effective fluoride varnish to prevent dental caries.