• 통상정보연구
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• 제13권4호
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• pp.23-49
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• 2011

본 연구는 고객관계관리의 중요성과 비중이 높아짐에 따라 B2B e-마켓플레이스에서의 e-CRM요인이 고객관계의 질과 성과에 미치는 영향관계를 파악하고 이러한 영향관계에서 전환장벽이 조절효과를 가지는지를 기업 대상 설문조사 자료를 토대로 실증분석 하였다. 분석결과를 살펴보면 첫째, e-CRM 요인 (e-Marketing, e-Service, e-Sales)은 B2B e-마켓플레이스 이용 고객들(공급자/구매자)과의 관계의 질(만족, 신뢰)과 성과(재구매 의도, 장기지향성)에 유의한 정(+)의 영향을 미쳤다. 둘째, 고객관계의 질과 성과 간의 분석에서는 관계의 질이 높을수록 성과도 높아지는 긍정적 관계를 나타냈다. 셋째, 고객관계의 질과 성과간의 관계에서 전환장벽(대안 매력도, 전환비용)의 조절효과 분석에서는 대안매력도와 전환비용은 모두 정(+)의 조절효과를 보였다.

• Nutritional Sciences
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• 제9권1호
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• pp.9-13
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• 2006

The differential effects of various fatty acids such as n-3 and n-6 types or degrees of unsaturation on the CYP2E1 induction and the production of lipid peroxidation (LPO) were investigated. The CYP2E1-transduced human hepatoma HepG2 cells (E47) were cultured in RPMI 1640 media containing different concentrations of various fatty acids up to 48 h incubation compared to 04 cells and CYP2E1-null cells. Treated fatty acids were linoleic acid (LA:n-6, C18:2), arachidonic acid (AA:n-6, C20:4) and docosahexaenoic acid (DHA:n-3, C22:6). The cell survival rate was decreased corresponding to the degree of unsaturation (LA>AA $\cong$DHA) and to LPO production in E47 and 04 cells. The four or five unsaturation degree of fatty acids, AA and DHA, caused time- and dose-dependent cell death in E47 cells but not as much as in C34 (without CYP2E1), suggesting an important role of CYP2E1 in the DHA mediated damage. In the levels of lipid peroxides (LPO), AA also elevated LPO by 3- and 5- fold compared to DHA or LA treated E47 cells. However, AA did not increase LPO until 48 h incubation in C34 cells. In conclusion, the polyunsaturated fatty acids induced CYP2E1 induction might be changed by the elevated levels of lipid peroxide (LPO) and oxidative stress through the connection of CYP2E1 and degrees of unsaturated fatty acids.

• 한국가축번식학회지
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• 제6권1호
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• pp.31-35
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• 1982

A study of 188 lactations of Holstein cows in Gwangju area was undertaken to establish the shape of lactation curve during the period from October in 1980 to January in 1982. The Gammafunction described by Wood(1967) was fitted to the lactations observed. The results obtained in the present study were summarized as follows; 1. The lactation curve of the 188 lactations was expressed by the equation based on Wood's model (1967) as follows; Yn=24.5m0.0762e-0.0944n(R2=0.99) 2. The lactation curves by parity were represented by the equations as follows; Yn=18.81n0.1486e-0.0741n(R2=0.98)……………parity 1 Yn=26.51n0.1161e-0.1200n(R2=0.96)……………parity 2 Yn=26.95n0.2804e-0.1703n(R2=0.99)……………parity 3 Yn=27.92n0.1429e-0.1427n(R2=0.98)……………parity 4 Yn=22.61n0.1985e-0.1211n(R2=0.94)……………parity 5 3. The lactation curves by calving seasons were represented by the equationes as follows; Yn=27.05n0.0739e-0.1005n(R2=0.98)……………spring Yn=23.08n0.2040e-0.1202n(R2=0.98)……………summer Yn=26.81n0.0460e-0.1134n(R2=0.98)……………autumn Yn=23.40n0.1299e-0.1101n(R2=0.95)……………winter

• 생명과학회지
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• 제23권7호
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• pp.941-946
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• 2013

신경세포의 가지돌기 내 단백질합성은 필요한 단백질을 실시간으로 제공할 수 있는 이점을 제공한다. 본 연구에서는 단백질합성인자 eIF4E와 그 억제 단백질인 eIF4EBP1의 발생단계별 표현을 배양한 해마신경세포를 면역 염색하여 조사하였다. eIF4E는 가지돌기에 점박이 모양으로 표현되었으며, 핵에는 표현되지 않았다. 그러나 eIF4EBP1는 가지돌기 뿐 아니라 발생초기(DIV 0.5)부터 핵에서 표현되었으며 성숙한 세포에서 핵에 더욱 뚜렷이 표현되었다. eIF4E 혹은 eIF4EBP1의 PSD95과의 colocalization은 $39.1{\pm}9.6%$ 및 $70.5{\pm}5.2%$ (DIV 7), $57.7{\pm}8.2%$ 및 $36.0{\pm}3.1%$ (DIV 10), $29.9{\pm}2.9%$ 및 $40.2{\pm}11.7%$ (DIV 20)이었다. eIF4E와 eIF4EBP1의 colocalizatin은 $18.5{\pm}2.6%$ (DIV 7), $11.1{\pm}3.9%$ (DIV 10), $38.6{\pm}5.6%$ (DIV 20)이었다. 이 결과는 eIF4E 및 eIF4EBP1의 많은 부분이 연접후에 위치하며, 발생초기에는 eIF4E가 활동적인 형태로 존재하지만, 성숙 신경세포에서는 eIF4EBP1과 결합하여 비활성적인 형태로 존재함을 의미한다.

• 대한지역사회영양학회지
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• 제13권5호
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• pp.713-722
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• 2008

The purpose of this study was to investigate the association among nutrient intakes and health-related lifestyles with cardiovascular disease risk assessed by blood lipid profile according to Apolipoprotein E genotypes. Middle-aged industrial male workers who had completed their annual medical examination were recruited and data of 675 subjects who finished the nutrient survey were used in the analysis. Anthropometric parameters, dietary assessment (FFQ), health-related lifestyles and blood profiles were used for statistical analyses. Apo E genotype groups were classified into the following three genotypes: Apo E2 group (including E2/E2, E2/E3, E2/E4), Apo E3 group (including E3/E3), Apo E4 group (including E3/E4, E4/E4). The frequency of Apo E2, E3, and E4 allele were 13.3%, 75.0% and 11.7% respectively. There were no significant differences in the anthropometric parameters depending on different Apo E genotypes. Also, no significant differences in the nutrient intakes were found according to the genotype groups. The nutrient intakes of all subjects were similar to or higher than the level of KDRIs (Dietary Reference Intakes For Koreans) except for intakes of calcium (67.44% of KDRIs), vitamin A (73.83% of KDRIs) and vitamin $B_2$ (78.02% of KDRIs). Also, there were no significant differences of health-related lifestyles according to Apo E genotype groups. As for the lipid profiles, Apo E4 group had significantly higher total and LDL-cholesterol concentrations than the Apo E2 group (p < 0.05). We confirmed that plasma total and LDL-cholesterol concentrations were greatly influenced by Apo E genotypes. However, nutrient intakes and health-related lifestyles were not associated with Apo E genotypes.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.969-978
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• 2014

The aprE2 gene with its prosequence from Bacillus subtilis CH3-5 was overexpressed in Escherichia coli BL21(DE3) by using plasmid pET26b(+). After IPTG induction, active and mature AprE2 was produced when cells were grown at $20^{\circ}C$, whereas inactive and insoluble enzyme was produced in a large amount when cells were grown at $37^{\circ}C$. The insoluble fraction was resuspended with 6 M guanidine-HCl and dialyzed against 2 M Tris-HCl (pH 7.0) or 0.5 M sodium acetate (pH 7.0) buffer. Then active AprE2 was regenerated and purified by a Ni-NTA column. Purified AprE2 from the soluble fraction had a specific activity of $1,069.4{\pm}42.4U/mg$ protein, higher than that from the renatured insoluble fraction. However, more active AprE2 was obtained by renaturation of the insoluble fraction. AprE2 was most stable at pH 7 and $40^{\circ}C$, respectively. The fibrinolytic activity of AprE2 was inhibited by PMSF, but not by EDTA and metal ions. AprE2 degraded $A{\alpha}$ and $B{\beta}$ chains of fibrinogen quickly, but not the ${\gamma}$-chain. AprE2 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe-pNA. The $K_m$ and $k_{cat}/K_m$ of AprE2 was 0.56 mM and $3.10{\times}10^4S^{-1}M^{-1}$, respectively.

• Molecules and Cells
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• 제27권3호
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• pp.329-336
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• 2009

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

• 대한수학회논문집
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• 제23권2호
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• pp.153-159
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• 2008

For $\mathbb{F}[e^{{\pm}x}]_{\{{\partial}\}}$, all the derivations of the evaluation algebra $\mathbb{F}[e^{{\pm}x}]_{\{{\partial}\}}$ is found in the paper (see [16]). For $M=\{{\partial}_1,\;{\partial}_1^2\},\;Der_{non}(\mathbb{F}[e^{{\pm}x}]_M))$ of the evaluation algebra $\mathbb{F}[e^{{\pm}x},\;e^{{\pm}y}]_M$ is found in the paper (see [2]). For $M=({\partial}_1^2,\;{\partial}_2^2)$, we find $Der_{non}(\mathbb{F}[e^{{\pm}x},\;e^{{\pm}y}]_M))$ of the evaluation algebra $\mathbb{F}[e^{{\pm}x},\;e^{{\pm}y}]_M$ in this paper.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
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• pp.719-723
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• 2007

SK커뮤니케이션즈가 운영하는 온라인 교육 사이트 이투스(www.etoos.com)가 웹2.0 시대에 맞춰 수강생들의 참여 공간을 늘리고, 온라인을 통해 '학습'과 '재미'를 강화한 개방형 홈페이지로 2007년 초 전면 개편되는 등 기존의 e-Learning 콘텐츠 회사들이 웹2.0 기반의 비즈니스 모델을 통해 콘텐츠 차별화를 꾀하려는 움직임이 매우 활발하다. 본 연구에서는 웹2.0 시대를 맞은 e-Learning 콘텐츠의 현황을 대표적인 중고등 교육 e-Learning 콘텐츠를 중심으로 분석해 보고, 차세대 e-Learning의 발전 방향을 효과적인 피드백이 제공되는 학습자 중심의 콘텐츠 개발과 웹2.0의 본질에 입각한 집단 지성을 이끌어내는 데서 찾고, 'e-Learning 콘텐츠 내의 virtual 개인 과외 선생님 community 형성에 관한 프로젝트'를 제안해 본 것이다.

• 경영과정보연구
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• 제34권1호
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• pp.81-101
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• 2015

본 논문은 온라인 구전의 성격을 설득 커뮤니케이션이 아닌 확산 커뮤니케이션 측면에 초점을 두고 온라인 구전 확산에 영향을 미치는 요인 및 영향관계를 검증하였다. 온라인 구전 확산과정은 크게 1차 구전(최초 구전전달)과 2차 구전(기존 구전정보의 재전달)으로 나눌 수 있으며 본 연구는 2차 구전의도에 미치는 온라인 구전의 정보적 특성의 영향을 밝히고자 하였다. 연구대상은 인터넷 환경에 익숙한 20대, 30대 젊은 층을 주로 하였으며, 소셜 미디어 사이트에 실제로 게시된 온라인 구전정보를 읽게 한 후 본 연구의 설문에 응답하도록 하였다. 연구결과, 온라인 구전의 동의성, 생생함, 중립성 모두 온라인 구전 신뢰성에 긍정적인 영향을 미치는 것으로 나타났으며, 온라인 구전 신뢰성은 2차 구전의도에 긍정적인 영향을 미치는 것으로 나타났다. 그리고 온라인 구전에 대한 관여도는 온라인 구전 신뢰성과 2차 구전의도간의 관계에서 조절변수 역할을 하는 것으로 나타났다. 이러한 연구결과를 기반으로 시사점 및 연구의 의의를 제시하였다.