• Title/Summary/Keyword: $E_{T(30)}$

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Preparation Method for Escherichia coliS30 Extracts Completely Dependent upon tRNA Addition to Catalyze Cell-free Protein Synthesis

  • Ahn, Jin-Ho;Hwang, Mi-Yeon;Oh, In-Seok;Park, Kyung-Moon;Hahn, Geun-Hee;Choi, Cha-Yong;Kim, Dong-Myung
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.5
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    • pp.420-424
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    • 2006
  • A simple method for depleting E. coliS30 extracts of endogenous tRNA has been developed. An $ethanolamine-Sepharose^{(R)}$ column equilibrated with water selectively captured the tRNA molecules in E. coli S30 extracts. As a result, S30 extracts filtered through this column became completely dependent upon the addition of exogenous tRNA to mediate cell-free protein synthesis reactions. We anticipate that the procedures developed and described will be particularly useful for in vitro suppression reaction studies designed to introduce unnatural amino acids into protein molecules.

Studies on the Organization and Transcription of Aspergillus nidulans tRNA Genes (Aspergillus nidulans의 tRNA 유전자의 구성과 발현에 관한 연구 II. Aspergillus nidulans 총 tRNA 유전자의 cloning)

  • 이병재;강현삼
    • Korean Journal of Microbiology
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    • v.21 no.4
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    • pp.229-237
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    • 1983
  • Total tRNA genes from Aspergillus nidulans were cloned for the further investigation of the structure and expression of Aspergillus tRNA genes. Aspergillus DNA was isolated from spores and cloned into pBR322 plasmid DNA using BamHI and $T_4$ ligase. The recombinant hybrid DNA was transformed into E. coli HB101 and some 30,000 transformants were initially selected. Of these, about 5,300 E. coli clones containing Aspergillus DNA inserted into plasmid pBR322 at BamHl site have been isolated. The hybridization data obtained from the labeled Aspergillus $^{32}P-tRNA$ indicated that 105 colonies carried the total tRNA genes. From the data above and cohybridization experiment, tRNA genes of Aspergillus nidulans seem to be twice more clustered than those of yeast.

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Thorea indica sp. nov. (Thoreales, Rhodophyta) from Uttar Pradesh, India

  • Necchi, Orlando Jr;Paiano, Monica O.;West, John A.;Ganesan, E. K.;Goer, Susan Loiseaux-de
    • ALGAE
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    • v.30 no.4
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    • pp.265-274
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    • 2015
  • Thorea indica sp. nov. is described from the Sai River, Uttar Pradesh, India (26°39′00.7″ N, 80°47′38.3″ E). Its classification is based on molecular sequences of the plastid-encoded RuBisCO large-subunit gene, rbcL and the barcode region of the mitochondrial encoded cytochrome c oxidase subunit 1, cox1, and morphological data. The sequence analyses confirm a new species of Thorea. The cox1 barcode sequence had 90.4-90.8% identity with Thorea sp. from Australia and Thorea hispida from Hawaii and China. Based on rbcL sequences the Indian specimen was positioned in a major clade with high support (>95 bootstrap and 0.95 posterior probability) containing two other species: T. okadae from Japan and T. hispida from the continental USA, Hawaii, the UK, and China. The divergences among these sequences were T. indica vs. T. okadae (2.8%) and T. indica vs. T. hispida (2.9-3.4%). The comparison of morphological characters of Thorea from India was not conclusive due to the inadequate descriptions in previous reports: most specimens reported as T. hispida fit within the circumscription of T. indica as described here. The previous report of T. siamensis from the Sai River is incorrect and the specimens fit within our description of T. indica. Thorea indica and T. okadae can be distinguished by minor morphometric characters and sexuality (dioecious vs. monoecious).

Growth curve estimates for wither height, hip height, and body length of Hanwoo steers (Bos taurus coreanae)

  • Park, Hu-Rak;Eum, Seung-Hoon;Roh, Seung-Hee;Sun, Du-Won;Seo, Jakyeom;Cho, Seong-Keun;Lee, Jung-Gyu;Kim, Byeong-Woo
    • Korean Journal of Agricultural Science
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    • v.44 no.3
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    • pp.384-391
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    • 2017
  • Growth curves in Hanwoo steers were estimated by Gompertz, Von Bertalanffy, Logistic, and Brody nonlinear models using growth data collected by the Hanwoo Improvement Center from a total of 6,973 Hanwoo (Bos taurus coreanae) steers 6 to 24 months old that were born between 1996 and 2015. The data included three parameters: A, mature size of body measurement; b, growth ratio; and, k, intrinsic growth rate. Nonlinear regression equations for wither height according to Gompertz, Von Bertalanffy, Logistic, and Brody models were $Y_t=144.7e^{-0.5869e^{-0.00301t}}$, $Y_t=145.3(1-0.1816e^{-0.00284t})^3$, $Y_t=143.1(1+0.7356e^{-0.00352t})^{-1}$, and $Y_t=146.8(1+0.4700e^{-0.00249t})^1$, respectively, while those for hip height were $Y_t=144.5e^{-0.5549e^{-0.00312t}}$, $Y_t=145.0(1-0.1724e^{-0.00295t})^3$, $Y_t=143.1(1+0.6863e^{-0.00360t})^{-1}$, and $Y_t=146.2(1+0.4501e^{-0.00263t})^1$, respectively. Equations for body length $Y_t=174.1e^{-0.8342e^{-0.00289t}}$, $Y_t=175.8(1-0.2500e^{-0.00265t})^3$, $Y_t=170.0(1+1.1548e^{-0.00363t})^{-1}$, and $Y_t=180.3(1+0.6077e^{-0.00215t})^1$, respectively, for the same models. Among the four models, the Brody model resulted in the lowest mean square error, with mean square errors of 31.79, 30.57, and 42.13, respectively, for wither height, hip height, and body length. Also, an estimated birth wither height, birth hip height, and birth body length (77.98, 80.57, and 70.97 cm, respectively) were lower in the Brody model than in other models. An inflection point was not observed during the growth phase of Hanwoo steer according to the growth curves calculated using Gompertz, Von Bertalanffy, and Logistic models. Based on the results, we concluded that the regression equation using the Brody model was the most appropriate among the four growth models. To obtain more accurate parameters, however, using data from a wider production period (from birth to shipping) would be required, and the development of a suitable model for body conformation traits would be needed.

Effect of [6]-Gingerol, a Pungent Ingredient of Ginger, on Osteoblast Response to Extracellular Reducing Sugar

  • Kim, Young-Ho;Nguyen, Huu Thng;Ding, Yan;Park, Sang-Heock;Choi, Eun-Mi
    • Food Science and Biotechnology
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    • v.16 no.5
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    • pp.807-811
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    • 2007
  • Diabetes is marked by high glucose levels and is associated with decreased bone mass and increased fracture rates. To determine if [6]-gingerol could influence osteoblast dysfunction induced by 2-deoxy-D-ribose (dRib), osteoblastic MC3T3-E1 cells was treated with dRib and [6]-gingerol and markers of osteoblast function and oxidized protein were examined. [6]-Gingerol ($10^{-7}\;M$) significantly increased the growth of MC3T3-E1 cells in the presence of 30 mM dRib (p<0.05). [6]-Gingerol ($10^{-7}\;M$) caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. We then examined the effect of [6]-gingerol on the production of osteoprotegerin and protein carbonyl in osteoblasts. Treatment with [6]-gingerol ($10^{-9}$ and $10^{-7}\;M$) increased osteoprotegerin secretion in osteoblastic cells. Moreover, [6]-gingerol ($10^{-9}$ and $10^{-7}\;M$) decreased protein carbonyl contents of osteoblastic MC3T3-E1 cells in the presence of 30 mM dRib. Taken together, these results demonstrate that [6]-gingerol inhibits dRib-induced damage and may be useful in the treatment of diabetes related bone diseases.

OSCULATING VERSUS INTERSECTING CIRCLES IN SPACE-BASED MICROLENS PARALLAX DEGENERACIES

  • Gould, Andrew
    • Journal of The Korean Astronomical Society
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    • v.52 no.4
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    • pp.121-131
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    • 2019
  • I investigate the origin of arc degeneracies in satellite microlens parallax ${\pi}_E$ measurements with only late time data, e.g., t > $t_0+t_E$ as seen from the satellite. I show that these are due to partial overlap of a series of osculating, exactly circular, degeneracies in the ${\pi}_E$ plane, each from a single measurement. In events with somewhat earlier data, these long arcs break up into two arclets, or (with even earlier data) two points, because these earlier measurements give rise to intersecting rather than osculating circles. The two arclets (or points) then constitute one pair of degeneracies in the well-known four-fold degeneracy of space-based microlens parallax. Using this framework of intersecting circles, I show that next-generation microlens satellite experiments could yield good ${\pi}_E$ determinations with only about five measurements per event, i.e., about 30 observations per day to monitor 1500 events per year. This could plausibly be done with a small (hence cheap, in the spirit of Gould & Yee 2012) satellite telescope, e.g., 20 cm.

Responses of high-rise building resting on piled raft to adjacent tunnel at different depths relative to piles

  • Soomro, Mukhtiar Ali;Mangi, Naeem;Memon, Aftab Hameed;Mangnejo, Dildar Ali
    • Geomechanics and Engineering
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    • v.29 no.1
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    • pp.25-40
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    • 2022
  • In this study, 3D coupled-consolidation numerical parametric study was conducted to predict the deformation mechanism of a 20 storey building sitting on (4×4) piled raft (with length of piles, Lp=30 m) to adjacent 6 m diameter (D) tunnelling in stiff clay. The influences of different tunnel locations relative to piles (i.e., zt/Lp) were investigated in this parametric study. In first case, the tunnel was excavated near the pile shafts with depth of tunnel axis (zt) of 9 m (i.e., zt/Lp). In second and third cases, tunnels were driven at zt of 30 m and 42 m (i.e., zt/Lp = 1.0 and 1.4), respectively. An advanced hypoplastic clay model (which is capable of taking small-strain stiffness in account) was adopted to capture soil behaviour. The computed results revealed that tunnelling activity adjacent to a building resting on piled raft caused significant settlement, differential settlement, lateral deflection, angular distortion in the building. In addition, substantial bending moment, shear forces and changes in axial load distribution along pile length were induced. The findings from the parametric study revealed that the building and pile responses significantly influenced by tunnel location relative to pile.

Effect of complex mechanical stimuli for MC3T3-E1 cells (MC3T3-E1 세포에 대한 복합 기계적 자극의 영향)

  • Kang, Kyung-Shin;Lee, Seung-Jae;Cho, Dong-Woo
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1700-1703
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    • 2008
  • The purpose of this study was to examine the effects of various mechanical stimuli for MC3T3-E1 cells. Among the several mechanical stimulations, we focused on compressive stain and ultrasound. In this study, we developed a bioreactor capable of applying controlled stimuli to scaffolds. PLLA/PCL scaffold was fabricated by using salt-leaching method. We performed dynamic cell culture using preosteoblasts MC3T3-E1 cells with 1MHz, 30mW/cm2 ultrasound and 10% of compressive strain. Result of CCK-8 analysis at 1, 4, 7, 10 days showed that mechanical stimuli had no significant effect for cell proliferation. However, those stimuli influenced ALP(Alkaline phopatase) activity, which is one of differentiation marker.

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Comparative Study on the Effectiveness of Modified Kato's Cellophane Thick Smear and Stoll's Dilution Egg Counting Technique for Quantitative Fecal Examination of Helminth Eggs (윤충란 정량검사를 위한 Stoll 희석충란계산법과 Kato 후층도말변법의 비교)

  • 홍성종;우호춘
    • Parasites, Hosts and Diseases
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    • v.30 no.2
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    • pp.141-146
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    • 1992
  • A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E,C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were $47.86{\times}10^{0.87{\;}logM.C.T.S.},{\;}$ in A. lumbricoides, $41.69{\times}10^{0.82{\;}logM.C.T.S.}$ in T. trichiura and $63.10{\times}10^{0.85{\;}logM.C.T.S.}$ in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.

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Kinetic study about the effect of electric field and contact time of high voltage impulse on reduction of Ca2+ concentration (고전압 임펄스 공정의 전계와 접촉시간이 Ca2+ 농도 저감에 미치는 영향의 속도론 연구)

  • Kim, Dam-Ha;Chang, In-Soung
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.2
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    • pp.113-120
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    • 2021
  • High voltage impulse (HVI) has been gained attention as an alternative technique that could control the CaCO3 scale problems encountered in water main, pipe, cooling tower and heat exchanger vessels. The aim of this study was to investigate the effect of electric field (E) and contact time (t) of HVI on reduction of Ca2+ concentration at two different temperatures of 25℃ and 60℃. A kinetic model on the effect of E and t was investigated too. As the E and t increased, the Ca2+ concentration decreased more than that of the control (= no HVI). The Ca2+ concentration decreased up to 81% at 15 kV/cm at 60℃, which was nearly 2 times greater than the control. With these experimental data-set of reduction of Ca2+ concentration under different E and t, the kinetic model was developed. The relationship between E and t required to reduce the concentration of Ca2+ by 30% was modeled at each temperature. The empirical model equations were; E0.83· t = 60.3 at 25℃ and E0.08· t = 1.1 at 60℃. These equations state the products of En and t is always constant, which means that the required contact time can be reduced in accordance with the increment of E and vice versa.