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검색결과 586건 처리시간 0.033초

Effects of NMDA, AMPA and Kainate on the Release of Acetylcholine in Rat Hippocampal and Striatal Slices

  • Kim, Do Kyung;Lee, Se-Oul;Jung, Kyu-Yong;Kim, Jong-Keun;Choi, Bong-Kyu
    • The Korean Journal of Physiology and Pharmacology
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    • 제8권6호
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    • pp.301-305
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    • 2004
  • This study examined the effects of N-methyl-D-aspartate (NMDA), ${\alpha}-amino$-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate on basal and electrically-evoked release of acetylcholine (ACh) from the rat hippocampal and striatal slices which were preincubated with $[^3H]choline$. Unexpectedly, the basal and evoked ACh release were not affected at all by the treatment with NMDA $(3{\sim}100{\mu}M)$, AMPA $(1{\sim}100{\mu}M)$ or kainate $(1{\sim}100{\mu}M)$ in hippocampal slices. However, in striatal slices, under the $Mg^{2+}-free$ medium, $30{\mu}M$ NMDA increased the basal ACh release with significant decrease of the electrically-evoked releases. The treatment with $1{\mu}M MK-801 not only reversed the $30{\mu}M$ NMDA-induced decrease of the evoked ACh release, but also attenuated the facilitatory effect of $30\;{\mu}M$ NMDA on the basal ACh release. The treatment with either $30\;{\mu}M$ AMPA or $100\;{\mu}M$ kainate increased the basal ACh release without any effects on the evoked release. The treatment with $10{\mu}M$ NBQX abolished the AMPA- or kainate-induced increase of the basal ACh release. Interestingly, NBQX significantly attenuated the evoked release when it was treated with AMPA, although it did not affect the evoked release alone without AMPA. These observations demonstrate that in hippocampal slices, ionotropic glutamate receptors do not modulate the ACh release in cholinergic terminals, whereas in striatal slices, activations of ionotropic glutamate receptors increase the basal ACh release though NMDA may decrease the electrically-evoked ACh release.

대전과 충남 지역에서 채취한 야생화로부터 효모의 분리 및 동정 (Isolation and Identification of Yeasts from Wild Flowers of Daejeon City and Chungcheongnam-do in Korea)

  • 민진홍;현세희;강민구;이향범;김창무;김하근;이종수
    • 한국균학회지
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    • 제40권3호
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    • pp.141-144
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    • 2012
  • 자연환경에서 분포하고 있는 효모의 mycoflora를 확인하고 궁극적으로 이들을 고부가가치의 산업에 응용하기 위한 연구의 일환으로 대전과 인근 충남지역 일부의 야생화들을 채집하여 효모들을 분리, 동정하였다. 대전지역 야생화 42점에서 32종의 효모들을 54균주 분리하였고 이들 중 Cryptococcus 속균과 Phodotonula 속균 등이 우점균이었으며 Metschnikowia 속균과 같이 희귀 효모들을 20여종 분리하였다. 대전 인근의 충남 금산군과 논산군 일부지역의 야생화 28점에서 모두 18종의 효모들을 26균주 분리하였다. 이들 균 중에서 Candida 속균과 Pseudozyma 속균 등이 우점균으로 분리되었다.

Red Pepper Productivity and Soil Properties as Affected by Different Intervals of Side-dressing N and K Applications in Plastic Film House

  • Ahn, Byung-Koo;Im, Ga-Young;Kim, Kab-Cheol;Chon, Hyong-Gwon;Jeong, Seong-Soo;Lee, Jin-Ho
    • 한국토양비료학회지
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    • 제47권3호
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    • pp.147-154
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    • 2014
  • Consecutive pepper cultivation in plastic film houses may lead to salt accumulation because pepper is considered a heavy nutrient feeder. For this reason, appropriate methods of fertilizer application should be established. Thus, we investigated the effect of different intervals of side-dressing N and K fertilizer applications on soil and red pepper in a plastic film house. All the amounts of recommended compost and phosphorus fertilizer were applied as basal dressing. Cultivars of the pepper plant were Cheon-Ha-Dae-Se (CHDS) and NW-BiGaLim (NW-BGL). Nitrogen and potassium fertilizers were treated as side-dressing at different intervals, 22 times in every 10 days, 15 times in every 15 days, and 11 times in every 20 days. Soil pH decreased with decreasing the intervals of side-dressing applications, whereas electrical conductivity (EC) declined with the increasing fertilizer application intervals. In particular, EC value decreased by up to 75% with CHDS cultivar in the plot of 20 day-interval and with NW-BGL cultivar in the plot of 15 day-interval. The concentrations of available phosphorus in the soils increased with increasing the interval. The concentration of exchangeable $K^+$ increased but exchangeable $Ca^{2+}$ and $Mg^{2+}$ decreased in all the plots, except in the control plot. The concentrations of nitrogen and phosphorus in leaves of the pepper plants were lowest in the control plot. Potassium concentrations in the pepper leaves were high in the control plot and in the plots of CHDS with 10 day-interval and NW-BGL with 15 day-interval. Red pepper productivity was high in the plots of 10- and 15 day-intervals for CHDS cultivar and 15- and 20 day-intervals for NW-BGL cultivar. Therefore, the 15 day-interval of side-dressing N and K applications was considered as an appropriate method for cultivating pepper plants and protecting soil in plastic film houses.

경남 농경지 이용구조 변화요인 분석과 전망 (Analysis on the Factors of Structural Changes and Prospects for Agricultural Land Use in Gyeongsangnam-do)

  • 최세현;조재환;김은순
    • 농업생명과학연구
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    • 제46권6호
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    • pp.173-184
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    • 2012
  • 본 연구는 지역 농지자원의 효율적인 이용을 위해 경상남도 농경지의 이용구조의 변화요인을 분석하고 전망하는 것을 목적으로 하고 있다. 이를 위해 전체 작물을 쌀, 하계 식량작물류, 하계 채소 및 특용작물류, 과일, 보리쌀, 동계 노지채소류, 동계 시설채소류의 7개 작물 그룹으로 구분하였으며, 이들에 대한 작물별 경작지 배분모형을 개발하여 추정하였다. 추정결과 자체가격과 재배면적 간에는 양의 상관관계가 존재하였으며, 대체가격에 있어서는 하계작물의 경우 쌀과 과일 등 모든 작물간에 경합관계가 존재하고 있으며, 동계작물의 경우, 노지 채소류와 시설채소류간에 경합관계가 뚜렷한 것으로 나타났다. 한편 재배면적의 자체가격과 농업노임에 대한 탄성치도 연도별로 추정하였다. 마지막으로 시나리오 분석 하에 경남지역 작물의 총재배면적은 2010년 15만 9천 ha에서 2020년에는 14만 3천 ha~15만 3천 ha에 이를 것으로 전망된다. 작물그룹별로 쌀, 기타식량작물류, 보리쌀은 재배면적의 감소가 예상되며, 하계채소 특용작물류, 과일, 동계노지채소류, 동계시설채소류는 재배면적의 정체가 예상된다.

노인요양시설에 대한 한국노인의 인식 (Elderly Koreans' Perception of Nursing Homes)

  • 김세영
    • 지역사회간호학회지
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    • 제27권3호
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    • pp.242-253
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    • 2016
  • Purpose: This research aims to understand the perceptions of nursing homes from the perspective of the elderly, explores the themes of such perception and provides supporting evidence for developing nursing intervention that focuses on the subjects of care within nursing homes. Methods: This research is based on qualitative research in a phenomenological methodology. The research participants included elders aged 67~83 living at home in Jeonnam, Jeonbuk Provinces and G City. Data collection methodologies included in-depth interviews and observation of participants. The data were analyzed in the phenomenological methodology by Colaizzi. Results: As a result of the research, it was found that all significant statements about the Korean elders' perception of nursing homes fell within 3 categories with 9 major themes and 23 minor themes. The three observed categories for the Korean elders' perception on nursing homes were: 'places they do not like to be in', 'places they have to be in' and 'places that makes them feel fortunate as they have a place to go'. Conclusion: This research provides foundational tools that will provide a steer for changes in nursing environment, physiological support, programs and education so that the needs of the elderly can be met more sufficiently.

제주도의 특수일 전력수요에 대한 기온 민감도 분석 (Sensitivity Analysis of Temperature on Special Day Electricity Demand in Jeju Island)

  • 조세원;박래준;김경환;권보성;송경빈;박정도;박해수
    • 전기학회논문지
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    • 제67권8호
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    • pp.1019-1023
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    • 2018
  • In this paper sensitivity analysis of temperature on special day electricity demand of land and Jeju Island is performed. The basic electricity demand per 3 hours is defined as electricity demand that reflects the GDP effect without the temperature influence. The temperature sensitivity per 3 hours is calculated through the relationship between special day electricity demand normalized to basic electricity demand and temperature. In the future, forecast error will be improved if the temperature sensitivity per 3 hours is applied to the special day load forecasting.

Structural and Optical Properties of Sol-gel Derived MgxZn1-x Thin Films

  • Kim, In-Soo;Kim, Do-Yun;Choi, Se-Young
    • 한국재료학회지
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    • 제19권3호
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    • pp.125-131
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    • 2009
  • In this report, the structural and optical properties of sol-gel derived $Mg_xZn_{1-x}O$ thin films upon changes in the composition and annealing temperature were investigated. The $Mg^{2+}$ content and the annealing temperature were varied in the range of $0{\leq}x{\leq}0.35$ and $400^{\circ}C{\leq}T{\leq}600^{\circ}C$, respectively. The films exhibited a hexagonal wurtzite structure of a polycrystalline nature. The optical transmittance exceeded 85% and the optical band gap of the film was tuned as high as 3.84 eV at a value of x = 0.35 (annealed at $400^{\circ}C$), which was evidently the maximum $Mg^{2+}$ content for the single-phase polycrystalline $Mg_xZn_{1-x}O$ thin films prepared in this experiment. The optical band gap and photoluminescence emission were tailored to the higher energy side while maintaining crystallinity without a significant change of the lattice constant.

초미세 결정립을 가지는 몰리브덴의 제조 및 기계적 특성 (Fabrication of Ultra Fine Grained Molybdenum and Mechanical Properties)

  • 김세훈;서영익;김대건;석명진;김영도
    • 한국분말재료학회지
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    • 제17권3호
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    • pp.235-241
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    • 2010
  • Mo nanopowder was synthesized by ball-milling and subsequent hydrogen-reduction of $MoO_3$ powder. To fabricate ultra fine grained molybdenum, two-step sintering and spark plasma sintering process were employed. The grain size of specimen by two-step sintering and spark plasma sintering was around $0.6\;{\mu}m$ and $0.4\;{\mu}m$, respectively. Mechanical properties of ultra fine grained Mo with relative density of above 90% were significantly improved at room and high temperatures comparing to commercial bulk Mo of 99% relative density. This result was mainly explained by the grain size refinement due to diffusion-controlled sintering.

Hydrochemical Characteristics and Nitrate-Nitrogen Contamination in Shallow Groundwater in Two Agricultural areas in Korea

  • Sul-Min Yun;Hang-Tak Jeon;Ji-Min Hwang;MoonSu Kim;HyunKoo Kim;Se-Yeong Hamm
    • 한국지구과학회지
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    • 제44권4호
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    • pp.291-306
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    • 2023
  • Shallow groundwater in rural areas is primarily polluted by agricultural activities. Nitrate-nitrogen is an indicator of artificial pollution. In this study, the hydrochemical characteristics and nitrate-nitrogen pollution of shallow groundwater were examined in two agricultural villages (Hyogyo-ri and Sinan-ri) in Chungcheongnam-do Province, Korea. Physicochemical quality analysis of shallow groundwater and stream water in the field, and chemical analysis in the laboratory were conducted from July 2020 to October 2021. In Hygyo-ri and Sinan-ri villages, shallow groundwater mainly belonged to the Ca-Cl, Ca-H CO3, Na-HCO3, and Na-Cl types, whereas stream water predominantly belonged to the Ca-HCO3 type. The nitrate-nitrogen concentration in shallow groundwater varied depending on the season, displaying an increased concentration of nitrate-nitrogen in the dry season compared to the rainy season. Stream water may be influenced by runoff into villages from the surrounding area, although both shallow groundwater and stream water are affected by artificial pollution. In addition, the nitrate-nitrogen concentration in stream water was lower than that in shallow groundwater.

Changes of anthocyanidin, growth characteristics and brown rice yield of red colored rices at two region of eastern coast in Gyeongbuk province

  • Kim, Sang Kuk;Shin, Jong Hee;Kim, Se Jong
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.330-330
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    • 2017
  • The tallest culm length was observed in Geonganghongmi cultivar grown in coast area, and culm length was always taller in coast area than that of inland area among these three red rice cultivars. However, number of tiller per rice plant was not affected by different cultural area. Number of spikelets per panicle was much more increased in inland area. Regional difference in number of spikelets per panicle was only observed in Jeongjinju cultivar. Among these red rice cultivars, the highest brown rice yield was Jeongjinju rice cultivar having 702kg in inland area and 692kg in coast area, respectively. The $L^*$ values, which expresses the brightness, were in the range of 36.3 ~ 36.7, 36.7 ~ 37.0, and 38.7 ~ 39.6 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. The $a^*$ values, which expresses the redness positively and greenness negatively were in the range of 12.3 ~ 12.8, 12.2 ~ 12.3, and 12.1 ~ 11.9 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. In addition, the b values, which expresses the yellowness positively and blueness negatively were in the range of 13.8 ~ 14.0, 13.6 ~ 13.5, and 14.9 ~ 14.7 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. Anthocyanidin content ranged 524 to $610{\mu}g/g$ dry weight basis. Cyanidin content was 11.4 to 14.0 times higher than that of delphinidin under coast and inland area. Among these rice cultivars, anthocyanidin content was always higher in rice cultivar grown at coast area. Highest head brown rice rate was only observed in Geonganghongmi to 95.2 at coast area and 95.4 inland area, respectively. Protein content was always higher in coast area than that of inland area. The lowest protein content was measured in Jeogjinju and amylose content was relatively increased in inland area compared to that of coast area. Consequently, in considered with brown rice yield and pigment content, Hongjinju rice cultivar was recommended in optimal pigment rice cultivar in eastern coast of Gyeongsangbuk-do Province.

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