• 한국습지학회지
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• 제9권2호
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• pp.71-84
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• 2007

마산만은 한국의 대표적인 반 폐쇄성 내만으로 유속이 느리며, 해수의 순환이 제한되어 입자물질의 침강이 주로 일어나고 있다. 특히, 육상기원 오염물질 유입은 환경오염문제를 일으켜, 부영양화, 적조, 저층 빈산소 현상 등의 문제가 빈번히 발생하고 있다. 본 연구에서는 생태계 모델을 이용하여, 이매패류를 포함하는 생태계 시스템에서 물질순환 과정의 계산을 수행하였으며, 여과식자인 이매패류에 의한 COD와 식물플랑크톤 같은 입자유기물질의 제거 효과도 계산하였다. 또한, 이매패류 양식장의 입식위치와 입식량을 산정하여, 생물학적 수질관리에 적용하고자 하였다. 모델결과에서, 최적 입식위치는 해양 물리적 과정을 참고로 하여 식물플랑크톤의 집적이 일어나는 곳을 선정하였으며, 최적 입식밀도는 35 개체/$m^{-3}$였고, 최적 입식 면적은 약 500 ha로 나타났다. 이매패류가 최적의 성장을 보인다고 가정하면, 육상부하의 삭감에 관한 노력 없이도 수중의 COD 농도의 18%의 개선효과가 나타났다.

• Biomolecules & Therapeutics
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• 제22권3호
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• pp.246-253
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• 2014

Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, $Ca^{2+}$ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.

• Molecules and Cells
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• 제26권3호
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• pp.236-242
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• 2008

Invertase (${\beta}$-D-fructofuranosidase; EC 3.2.1.26) catalyzes the conversion of sucrose into glucose and fructose and is involved in an array of important processes, including phloem unloading, carbon partitioning, the response to pathogens, and the control of cell differentiation and development. Its importance may have caused the invertases to evolve into a multigene family whose members are regulated by a variety of different mechanisms, such as pH, sucrose levels, and inhibitor proteins. Although putative invertase inhibitors in the Arabidopsis genome are easy to locate, few studies have been conducted to elucidate their individual functions in vivo in plant growth and development because of their high redundancy. In this study we assessed the functional role of the putative invertase inhibitors in Arabidopsis by generating transgenic plants harboring a putative invertase inhibitor gene under the control of the CaMV35S promoter. A transgenic plant that expressed high levels of the putative invertase inhibitor transcript when grown under normal conditions was chosen for the current study. To our surprise, the stability of the invertase inhibitor transcripts was shown to be down-regulated by the phytohormone ABA (abscisic acid). It is well established that ABA enhances invertase activity in vivo but the underlying mechanisms are still poorly understood. Our results thus suggest that one way ABA regulates invertase activity is by down-regulating its inhibitor.

• 약학회지
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• 제55권3호
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• pp.227-233
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• 2011

We previously reported the protein isolated from Coptidis Rhizoma (CRP), which has antifungal activity against a fungal pathogen, Candida albicans. In the current study, we investigated what portion in the CRP is responsible for the antifungal activity. For the investigation, the CRP was fractionated on a Shepadex G-50 column. Data resulting from the fractionation, seven fractions were obtained. Fractions (Fr.) I, II, and III eluted initially from the column showed no inhibitory effect on the growth of C. albicans, whereas Fr. IV, V, and VI eluted later revealed inhibition of the growth, and Fr. IV and VI showed potent antifungal activity by broth susceptibility analysis. However, Fr. VI was contained in the CRP more than Fr. IV, which led us to select the VI for the following experiments. In a murine model of a subcutaneous candidiasis caused by C. albicans, the Fr. VI displayed a therapeutic effect on nude mice pretreated with anti-neutrophil monoclonal antibody (RB68C5) and then infected subcutaneously with live C. albicans. At day 16, these mice were healed almost up to 78% of the infected area when compared to infected area of control nude mice that received diluent (Dulbecco's Phosphate-Buffered Saline; DPBS), instead of the Fr. VI (P<0.01). The Fr. VI blocked hyphal formation from blastoconidial form of C. albicans (P<0.01), which might prevent penetration of hyphae to the deeper site of skin and thus helps the healing. In the ionic strength test, the effect of Fr. was influenced by $Ca^{2+}$ ion just like other known antimicrobial peptides, but the influence was affected at an extremely high concentration such as 500 mM. Thus, such ion-concentration is considered to be meaningless in the clinical situation. Considering all data together, Coptidis Rhizoma is appeared to produce an antimicrobial peptide that has therapeutic effect on subcutaneous infection caused by C. albicans.

• KSBB Journal
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• 제17권1호
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• pp.20-25
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• 2002

We performed a basic experiment for rapid, on-line, real-time measurement of HBsAg by using a surface plasmon resonance biosensor to quantify the recognition and interaction of biomolecules. We immobilized the anti-HBsAg polyclonal antibody to the dextran layer on a CM5 chip surface which was pre-activated by N-hydroxysuccinimide for amine coupling. The binding of the HBsAg to the immobilized antibody was measured by the mass increase detected by the change in the SPR signal. The binding characteristics between HBsAg and its antibody followed typical monolayer adsorption isotherm. When the entire immobilized antibody was interacted, there was no additional, non-specific binding observed, which suggested the biointeraction was very specific as expected and independent of the ligand density. No significant steric hindrance was observed at 17.6 nm/$mm^2$ immobilization density. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the chip surface was linear up to ca. $40\mu\textrm{g}$/mL, which is much wider than that of the ELISA method. It appeared the antigen-antibody binding was increased as the immobilized ligand density increased, but verification is warranted. This study showed the potential of this biosensor-based method as a rapid, simple, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification replacing the current ELISA method.

• 한국전기전자재료학회논문지
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• 제21권9호
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• pp.835-843
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• 2008

We have fabricated the source-drain electrodes for OTFTs by screen printing method and manufactured Ag pastes as conductive paste. To obtain excellent conductivity and screen-printability of Ag pastes, the dispersion characteristics of Ag pastes prepared from two types of acryl resins with different molecular structures and Ag powder treated with caprylic acid, triethanol amine and dodecane thiol as surfactant respectively were investigated. The Ag pastes containing Ag powder treated with dodecane thiol having thiol as anchor group or AA4123 with carboxyl group(COOH) of hydrophilic group as binder resin exhibited excellent dispersity. But, Ag pastes(CA-41, TA-41, DT-41) prepared from AA4123 fabricated the insulating layer since the strong interaction between surface of Ag powder and carboxyl group(COOH) of AA4123 interfered with the formation of conduction path among Ag powders. The viscosity behavior of Ag pastes exhibited shear-thinning flow in the high shear rate range and the pastes with bad dispersion characteristic demonstrated higher shear-thinning index than those with good dispersity due to the weak flocculated network structure. The output curve of OTFT device with a channel length of 107 ${\mu}m$ using screen-printed S-D electrodes from DT-30 showed good saturation behavior and no significant contact resistance. And this device exhibited a saturation mobility of $4.0{\times}10^{-3}$ $cm^2/Vs$, on/off current ratio of about $10^5$ and a threshold voltage of about 0.7 V.

• 한국식품조리과학회지
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• 제20권1호
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• pp.100-111
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• 2004

The purpose of this study was to investigate the status of current nutrition labeling on the packaging of the processed foods that provide consumers with a reliable and consistent source of information, which has been considered as a useful aid for food selection and a potent educational tool for nutrition in daily life. The 2,160 processed foods purchased at the supermarket on September, 2002, were divided by food category issued from the 2002 food codes and assessed in the terms of the nutrition composition labeling and nutrition claims. Nutrition composition labeling was found on 356 of the 2160 processed foods items. Milk and dairy products had 49.7％ of nutrition composition labeling, which was the largest number among the food category. Tables were most frequently used as the type of nutrition composition labeling (79.8％). Nutrition composition including many different ways of expression, such as a table of nutrition composition, indication of nutrition composition, analysis table of nutrition composition and comparative table of nutrition composition, made frequent use of nutrition composition labeling titles (78.7％). The various unit of measures were use in the nutrition labeling of the processed foods, per l00g or 100$m\ell$ was the highest (44.6％) under the currently practiced nutrition labeling. The correct labeling standard with nutrient content and ％ RDA except energy, was used on 47.8％ of labels, and those with only liability indication nutrient and liability indication nutrients plus discretion indication nutrients were 25.3 and 22.5％ respectively. The processed foods with nutrition claims were 8.0％ (172 items). Nutrition claims were divided in two ways: nutrient content claims and nutrient comparative claims. The most frequently used claims were contained in the former (44.4％) and more or plus in the latter case (16.3％). Ca was the most popular item as a nutrition claim nutrient (50.6％).

• Current Research on Agriculture and Life Sciences
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• 제14권
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• pp.111-122
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• 1996

Streptomyces chibaensis J-59 xylanase는 CSL 배지(1% corn steep liquor, 0.15% glucose, 0.1% $MgSO_4{\cdot}7H_2O$, pH 7.0)에서 1% xylan을 효소생산 유도물질로 첨가하였을 경우에 생산(0.83 unit/ml) 되었으나, xylose를 비롯한 각 종 탄소원을 포함하는 배지에서는 효소가 생산되지 않았다. S. chibaensis로 부터 생산된 세포외 xylanase를 $(NH_4)_2SO_4$ 분획침전, DEAE-Sephadex A-50 column chromatography, Sephadex G-200 gel filtration 과정으로 약 29%의 수율로 20배 정제하였다. 정제한 xylanase는 SDS-PAGE 및 Sephadex G-200 gel filtraion에서 분자량이 모두 25,000 Da으로 나타나 monomenc enzyme으로 확인되었다. 금속이온에 대한 영향은 $Fe^{3+}$, $Hg^{2+}$, $Cu^{2+}$, sodium dodecyl sulfate, N-bromosuccinide 등에서는 아주 강한 저해를 받았고, $Mn^{2+}$, $Zn^{2+}$에서는 약간의 저해를 받았다. 반면에 $Mg^{2+}$는 효소활성을 증가시켰다. Xylanase에 의한 xylooligo 당의 생산양상을 TLC로 조사한 결과 xylobiose, xylotriose 및 xylotetrose가 주 생산물이었으며, 반응 1시간 이후부터 소량의 xylose가 생성되었다. 따라서 본 효소는 전형적인 endotype xylanase로 확인되었으며 새로운 기능성식품인 자일로 올리고당(xylooligo-saccharides)의 제조에 이용할 수 있을 것이다.

• 한국가금학회지
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• 제35권1호
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• pp.21-27
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• 2008

본 연구는 산란노계 사료 내 광물질 강화제의 추가적인 공급이 산란노계의 생산성, 계란 품질 및 혈청 내 칼슘, 인 농도에 미치는 영향에 대하여 알아보기 위해 수행하였다. 사양시험은 72주령 Hy-line Brown 240수를 공시하여 4주간 실시하였다. 1) CON(basal diet), 2) ESS1(basal diet + Egg shell strengthener 0.1%), 3) ESS2(basal diet + Egg shell strengthener 0.2%) 및 4) FESS(basal diet + Fe egg shell strengthener 0.2%)로 4개 처리를 하여 처리당 5반복, 반복당 12수씩 완전 임의 배치하였다. 전체 시험 기간 동안 산란율은 대조구 및 ESS2 처리구가 ESS1 처리구와 비교하여 유의적으로 높게 나타났으며(P<0.05), 난각 강도 및 종료시의 난각 두께는 ESS2 처리구가 대조구 및 FESS 처리구보다 유의적으로 높게 나타났다(P<0.05). 계란 품질에서 난황색은 ESS1 처리구가 다른 처리구와 비교하여 유의적으로 가장 낮게 나타났으며(P<0.05), 호우유닛은 ESS1 처리구 및 FESS 처리구가 대조구와 비교하여 유의적으로 낮았다(P<0.05). 미네랄 및 비타민 강화제의 첨가는 혈청 내 Ca 및 P 함량과 난각색에 영향을 미치지 못했다. 결론적으로, 산란노계 사료에 0.2%의 미네랄 및 비타민 강화제를 첨가한 ESS2 처리구는 난각 강도와 두께가 개선되는 효과를 나타내었다.

• 한국광물학회지
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• 제32권4호
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• pp.235-247
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• 2019

남해 섬진강 하구유역에 발달되어 있는 남해중앙니질대는 흑산머드벨트의 퇴적물들과 섬진강의 퇴적물들을 공급받는 것으로 알려져 있다. 그러나 니질 퇴적체를 형성하기 위해서는 위 지역에서 공급되는 퇴적물보다 더 많은 양의 퇴적물이 공급되어야하기 때문에, 추가적인 퇴적물 공급원에 대한 연구가 필요하다. 본 연구에서는 남해중앙니질대 퇴적물의 기원지 및 퇴적물 유입경로의 변화를 알아보기 위해, 16PCT-GC01 및 16PCT-GC03 코어에 대해 점토광물 및 주성분원소 분석을 수행하였다. 황하 퇴적물은 스멕타이트의 함량이 높고, 양쯔강 퇴적물은 일라이트의 함량이 높으며, 한국 강 퇴적물들은 카올리나이트와 녹니석의 함량이 높다. 또한 한국 강 퇴적물은 Al, Fe, K가 풍부하고, 중국 강 퇴적물은 Ca, Mg, Na 등이 풍부하다. 따라서 점토광물과 주성분원소를 이용해 퇴적물의 기원지를 추적할 수 있다. 연구 결과, 남해중앙니질대의 코어 퇴적층은 총 3개의 퇴적 단위(sediment unit)로 구분할 수 있다. 해수면이 가장 낮은 저수위기(lowstand stage)에 해당되는 퇴적 단위 3은 황하로부터 공급된 퇴적물이 연안류 혹은 조석 작용에 의해 연구지역으로 공급된 것으로 해석되고, 해수면이 빠르게 상승하는 해침기(transgressive stage)에 해당되는 퇴적 단위 2는 황하의 영향이 약해지고 양쯔강과 한국 강들의 영향이 강해지는 것으로 해석된다. 현재와 같은 해수면과 해류의 순환이 형성된 고수위기(highstand stage)에 해당되는 퇴적 단위 1은 양쯔강과 한국 강으로부터 퇴적물이 해류를 통해 연구지역으로 공급된 것으로 해석된다.