Hong, Sok Jin;Eom, Ki Hyuk;Jang, Ju Hyung;Park, Jong Su;Kim, Dong Myung;Kwon, Jung No
Journal of Wetlands Research
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v.9
no.2
/
pp.71-84
/
2007
Masan Bay is a representative semi-closed bay acted as a sedimentation reservoir with a slow current velocity and a poor water circulation in Korea. The pollutants from terrestrial sources into the Masan Bay have apparently environmental pollution problems, such as eutrophication, red tied, and hypoxia. In this study, An ecological modeling work was performed to estimate the material circulation including the growth of bivalve in ecosystem. Furthermore, the effect of water purification was calculated by filter feeding bivalve to particulate organic matter just like COD and phytoplankton. And Water quality management strategy by bio-purification of bivalve is derived through selection of location, quantification of bivalve aquaculture farm. The results showed that the optimum location for bivalve farming is where phytoplankton accumulation by physical processes is maximized and the optimum density and area of bivalve are 35 individuals $m^{-3}$ and ca. 500 hectare, respectively. When assuming conditions for the optimum growth of bivalve, COD could decrease by up to 18% even without other reduction of pollution loads.
Weon, Jin Bae;Yun, Bo-Ra;Lee, Jiwoo;Eom, Min Rye;Ko, Hyun-Jeong;Lee, Hyeon Yong;Park, Dong-Sik;Chung, Hee-Chul;Chung, Jae Youn;Ma, Choong Je
Biomolecules & Therapeutics
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v.22
no.3
/
pp.246-253
/
2014
Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, $Ca^{2+}$ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.
Invertase (${\beta}$-D-fructofuranosidase; EC 3.2.1.26) catalyzes the conversion of sucrose into glucose and fructose and is involved in an array of important processes, including phloem unloading, carbon partitioning, the response to pathogens, and the control of cell differentiation and development. Its importance may have caused the invertases to evolve into a multigene family whose members are regulated by a variety of different mechanisms, such as pH, sucrose levels, and inhibitor proteins. Although putative invertase inhibitors in the Arabidopsis genome are easy to locate, few studies have been conducted to elucidate their individual functions in vivo in plant growth and development because of their high redundancy. In this study we assessed the functional role of the putative invertase inhibitors in Arabidopsis by generating transgenic plants harboring a putative invertase inhibitor gene under the control of the CaMV35S promoter. A transgenic plant that expressed high levels of the putative invertase inhibitor transcript when grown under normal conditions was chosen for the current study. To our surprise, the stability of the invertase inhibitor transcripts was shown to be down-regulated by the phytohormone ABA (abscisic acid). It is well established that ABA enhances invertase activity in vivo but the underlying mechanisms are still poorly understood. Our results thus suggest that one way ABA regulates invertase activity is by down-regulating its inhibitor.
We previously reported the protein isolated from Coptidis Rhizoma (CRP), which has antifungal activity against a fungal pathogen, Candida albicans. In the current study, we investigated what portion in the CRP is responsible for the antifungal activity. For the investigation, the CRP was fractionated on a Shepadex G-50 column. Data resulting from the fractionation, seven fractions were obtained. Fractions (Fr.) I, II, and III eluted initially from the column showed no inhibitory effect on the growth of C. albicans, whereas Fr. IV, V, and VI eluted later revealed inhibition of the growth, and Fr. IV and VI showed potent antifungal activity by broth susceptibility analysis. However, Fr. VI was contained in the CRP more than Fr. IV, which led us to select the VI for the following experiments. In a murine model of a subcutaneous candidiasis caused by C. albicans, the Fr. VI displayed a therapeutic effect on nude mice pretreated with anti-neutrophil monoclonal antibody (RB68C5) and then infected subcutaneously with live C. albicans. At day 16, these mice were healed almost up to 78% of the infected area when compared to infected area of control nude mice that received diluent (Dulbecco's Phosphate-Buffered Saline; DPBS), instead of the Fr. VI (P<0.01). The Fr. VI blocked hyphal formation from blastoconidial form of C. albicans (P<0.01), which might prevent penetration of hyphae to the deeper site of skin and thus helps the healing. In the ionic strength test, the effect of Fr. was influenced by $Ca^{2+}$ ion just like other known antimicrobial peptides, but the influence was affected at an extremely high concentration such as 500 mM. Thus, such ion-concentration is considered to be meaningless in the clinical situation. Considering all data together, Coptidis Rhizoma is appeared to produce an antimicrobial peptide that has therapeutic effect on subcutaneous infection caused by C. albicans.
Lee, E. K.;Ahn, S. J.;Yoo, C. H.;Ryu, K.;Jeon, J. Y.;Lee, H. I.;Choi, S. C.;Lee, Y. S.
KSBB Journal
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v.17
no.1
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pp.20-25
/
2002
We performed a basic experiment for rapid, on-line, real-time measurement of HBsAg by using a surface plasmon resonance biosensor to quantify the recognition and interaction of biomolecules. We immobilized the anti-HBsAg polyclonal antibody to the dextran layer on a CM5 chip surface which was pre-activated by N-hydroxysuccinimide for amine coupling. The binding of the HBsAg to the immobilized antibody was measured by the mass increase detected by the change in the SPR signal. The binding characteristics between HBsAg and its antibody followed typical monolayer adsorption isotherm. When the entire immobilized antibody was interacted, there was no additional, non-specific binding observed, which suggested the biointeraction was very specific as expected and independent of the ligand density. No significant steric hindrance was observed at 17.6 nm/$mm^2$ immobilization density. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the chip surface was linear up to ca. $40\mu\textrm{g}$/mL, which is much wider than that of the ELISA method. It appeared the antigen-antibody binding was increased as the immobilized ligand density increased, but verification is warranted. This study showed the potential of this biosensor-based method as a rapid, simple, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification replacing the current ELISA method.
Journal of the Korean Institute of Electrical and Electronic Material Engineers
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v.21
no.9
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pp.835-843
/
2008
We have fabricated the source-drain electrodes for OTFTs by screen printing method and manufactured Ag pastes as conductive paste. To obtain excellent conductivity and screen-printability of Ag pastes, the dispersion characteristics of Ag pastes prepared from two types of acryl resins with different molecular structures and Ag powder treated with caprylic acid, triethanol amine and dodecane thiol as surfactant respectively were investigated. The Ag pastes containing Ag powder treated with dodecane thiol having thiol as anchor group or AA4123 with carboxyl group(COOH) of hydrophilic group as binder resin exhibited excellent dispersity. But, Ag pastes(CA-41, TA-41, DT-41) prepared from AA4123 fabricated the insulating layer since the strong interaction between surface of Ag powder and carboxyl group(COOH) of AA4123 interfered with the formation of conduction path among Ag powders. The viscosity behavior of Ag pastes exhibited shear-thinning flow in the high shear rate range and the pastes with bad dispersion characteristic demonstrated higher shear-thinning index than those with good dispersity due to the weak flocculated network structure. The output curve of OTFT device with a channel length of 107 ${\mu}m$ using screen-printed S-D electrodes from DT-30 showed good saturation behavior and no significant contact resistance. And this device exhibited a saturation mobility of $4.0{\times}10^{-3}$$cm^2/Vs$, on/off current ratio of about $10^5$ and a threshold voltage of about 0.7 V.
The purpose of this study was to investigate the status of current nutrition labeling on the packaging of the processed foods that provide consumers with a reliable and consistent source of information, which has been considered as a useful aid for food selection and a potent educational tool for nutrition in daily life. The 2,160 processed foods purchased at the supermarket on September, 2002, were divided by food category issued from the 2002 food codes and assessed in the terms of the nutrition composition labeling and nutrition claims. Nutrition composition labeling was found on 356 of the 2160 processed foods items. Milk and dairy products had 49.7% of nutrition composition labeling, which was the largest number among the food category. Tables were most frequently used as the type of nutrition composition labeling (79.8%). Nutrition composition including many different ways of expression, such as a table of nutrition composition, indication of nutrition composition, analysis table of nutrition composition and comparative table of nutrition composition, made frequent use of nutrition composition labeling titles (78.7%). The various unit of measures were use in the nutrition labeling of the processed foods, per l00g or 100$m\ell$ was the highest (44.6%) under the currently practiced nutrition labeling. The correct labeling standard with nutrient content and % RDA except energy, was used on 47.8% of labels, and those with only liability indication nutrient and liability indication nutrients plus discretion indication nutrients were 25.3 and 22.5% respectively. The processed foods with nutrition claims were 8.0% (172 items). Nutrition claims were divided in two ways: nutrient content claims and nutrient comparative claims. The most frequently used claims were contained in the former (44.4%) and more or plus in the latter case (16.3%). Ca was the most popular item as a nutrition claim nutrient (50.6%).
This study was conducted to evaluate the effects of mineral and vitamin-enhanced supplementation on egg production, egg quality and concentrations of calcium and phosphorus in serum of spent laying hens. A total of 240 (72-wk-old) Hyline brown commercial hens were used in the current trial for 4 weeks. Dietary treatments included 1) CON (basal diet), 2) ESS1 (basal diet + Egg shell strengthen 0.1%), 3) ESS2 (basal diet + Egg shell strengthen 0.2%) and 4) FESS (basal diet + Fe egg shell strengthen 0.2%). During the overall period, egg production was increased in CON and ESS2 treatments compared with ESS1 treatment (P<0.05). Egg shell breaking strength and final egg shell thickness were significantly higher in ESS2 treatment than CON and FESS treatments (P<0.05). Egg yolk color index was significantly lower in ESS1 treatment than other treatments (P<0.05). Moreover, Haugh unit was significantly lower in ESS1 and FESS treatments than CON treatment (P<0.05). Mineral- and vitaminenhanced supplementation did not affect either egg shell color or calcium and phosphorus concentration in serum. In conclusion, mineral- and vitamin- enhanced supplementations of laying hens diet are helpful in improving egg shell breaking strength and thickness by supplementation of Egg shell strengthener 0.2%.
Lee, Hong Geum;Park, Won Young;Koo, Hyo Jin;Choi, Jae Yeong;Jang, Jeong Kyu;Cho, Hyen Goo
Journal of the Mineralogical Society of Korea
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v.32
no.4
/
pp.235-247
/
2019
The Central South Sea Mud (CSSM), developed in the Seomjin River estuary, is known to be supplied with sediments from Heuksan Mud Belt (HMB) and Seomjin River. However, in order to form a mud belt, more sediments must be supplied than supplied in the above areas. Therefore, research on additional sources should be conducted. In this study, clay minerals, major elements analyzes were performed on cores 16PCT-GC01 and 16PCT-GC03 in order to investigate the transition in the provenance and transport pathway of sediments in CSSM. The Huanghe sediments are characterized by higher smectite and the Changjiang sediments are characterized by higher illite. Korean river sediments contain more kaolinite and chlorite than those of chinese rivers. Korean river sediments have higher Al, Fe, K concentraion than Chinese river sediments and Chinese rivers have higher Ca, Mg, Na than those of Korean rivers. Therefore, clay minerals and major elements can be a useful indicator for provenance. Based on our results, CSSM can be divided into three sediment units. Unit 3, which corresponds to the lowstand stage, is interpreted that sediments from Huanghe were supplied to the study area by coastal or tidal currents. Unit 2, which corresponds to the transgressive stage, is interpreted to have a weaker Huanghe effect and a stronger Changjiang and Korean rivers effect. Unit 1, which corresponds to the highstand stage when the sea level is the same as present and current circulation system is formed, is interpreted that sediments from Changjiang and Korean rivers are supplied to the research area through the current.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.14
no.2
/
pp.69-79
/
2009
Major and trace elements were analyzed in three core sediments to investigate geochemical characteristics of East Sea sediments and provenance changes during late Quaternary in Ulleung Basin. Comparing with Yellow and South Sea sediments, contents of major elements were generally similar while contents of trace elements were significantly different. Furthermore, within this basin, there were some variabilities in trace element compositions. In the western slope sediments (WS), Mo was enriched over 6 times as much as other sites. On the other hand, Zr, Nb, Hf and Ta were enriched in basin sediments (Basin), and Ca and Cs were enriched in southern slope sediments (SS). After excluding elements derived from biogenic, authigenic and diagenetic origins, the lithogenic elements (K, Ti, Cs, Zr, Nb, Hf and Ta) could be classified into three groups from the comparison of element/Al ratios among cores. The first group consisted of elements (K and Ti) that showed the nearly similar element/Al ratios among three cores. The second group contained Cs which showed significant difference between two slope sediments. The third group elements (Zr, Nb, Hf and Ta) showed highly enriched in basin relative to both slope areas. The depth profiles of metal/Al ratios in basin sediments provided the following interpretation for the compositions of sediment and their variation. From 10,000 yr B.P. to 7,000 yr B.P. two lithogenic components (volcanic ashes and western slope sediments) were mixed and deposited in the basin. After 7,000 yr B.P., however, southern slope sediments were mixed with volcanic ashes and deposited in basin area. This event of source change is nearly close to inflow period of the Tsushima Warm Current to Ulleung Basin. Thus, it might be suggested that element geochemistry in Ulleung basin sediment indicate the change of current system in the study area.
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