• 환경생물
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• 제17권2호
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• pp.199-204
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• 1999

여름철 자연조건에서 자라는 두릅나무과 식물 잎의 SOD활성과 광계II의 광화학적 효율의 차이를 분석하고 온도 스트레스와 paraquat의 영향을 조사하였다. 그 결과, 두릅나무과 식물 6종의 잎으로부터 총 8개의 superoxide dismutase(SOD) isoenzyme이 구분되었다. 그 중, 섬오갈피나무 (Acanthopanax koreanum)에서는 두릅나무과 식물에 공통적인 2개의 isoenzyme (SOD 4와 SOD 6)이 높은 활성으로 검출되었고, 황칠나무(Dendropanax morbifera)에서는 두릅나무과 식물이 갖는 8개의 isoenzyme이 모두 검출되었다. 광계II의 광화학적 효율을 나타내는 Fv/Fm은 낙엽성 식물에서는 섬오갈피나무가 다른 종보다 높았으며, 상록성 식물에서는 음지에 위치해 있는 잎이 양지에 위치해 있는 잎보다 전반적으로 높게 나타났다. 섬오갈피나무 잎 disc의 Fv/Fm은 온도가 높아짐에 따라 감소하였지만 황칠나무 잎 disc에서는 35$^{\circ}C$에서 현저하게 감소하였다. 그리고 온도 스트레스와 함께 paraquat를 처리하였을 때 섬오갈피나무에서는 4$^{\circ}C$에서, 황칠나무에서는 35$^{\circ}C$에서 Fv/Fm이 억제되었다. 한편, SOD 활성은 섬오갈피나무 잎 disc에서는 4$^{\circ}C$와 28$^{\circ}C$에서 높았고 paraquat를 함께 처리하였을 매에는 모든 온도에서 활성이 높아졌다. .그러나, 황칠나무 잎 disc에서는 온도가 높아짐에 따라 SOD활성이 억제되었고 paraquat를 함께 처리하였을 때에는 더욱 억제되었다. 따라서, 섬오갈피나무는 황칠나무에 비하여 온도 스트레스나 산화적 스트레스에 효율적으로 대처하는 것으로 나타났다.

• Journal of Ginseng Research
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• 제22권3호
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• pp.161-167
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• 1998

American ginseng plants (Panax quinquefolium L.) of various ages were harvested every two weeks during each of three growing seasons and dry matter yield of components and root respiration determined. Shoot dry weight was about 0.5 g, 2.5 g and 4 g for 2, 3 and 4-year-old plants, respectively and fruit dry weight was as much as 50% of this in 3- and 4-year-.old plants. Root dry weight decrease by 30~50% as shoots emerged and at the end of the season was about 2 g, 3.5 g and 5 g in 2, 3, 4 and 5-year-old plants, respectively. Shoot and root dry weight were linearly related with an approximate 1:2 ratio. Root respiration rate at 2$0^{\circ}C$ in the dark was about 5 $\mu\textrm{g}$ CO2 g-1 DW(dry weight) min-1 in the early season, then doubled within 50 days as shoots emerged, and thereafter declined over the season to 2~5 $\mu\textrm{g}$ CO2 g-1 DW min-1. The Q10 for dark respiration over the interval from 10 to 2$0^{\circ}C$ was 1.58. Root respiration rate and shoot growth rate was positively linearly related in all ages of plants. Key words: Dry weight, partitioning.

• 한국자원식물학회지
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• 제22권6호
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• pp.506-512
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• 2009

To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1974년도 학술대회지
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• pp.101-113
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• 1974

The radioactive compound sodium $acetate-U-C^{14}$ (C-14 acetate) was administered to two- and four-year-old July and September American ginseng (Panax quinquefolium L.) plants and cuttings. The C-14 acetate uptake was approximately $99\%.$ The autoradiochromatograms suggest that the saponins(panaquilins) isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration $(\%$ plant dry weight) of semipurified saponins were high in the leaves $(13.8\%),$ compared to fruits $(9.8\%),\;stems\;(7.9\%)\;and\;roots\;(6.3\%).$ The average percentage of C-14 acetate incorporation into panaquilins was $4.8\%.$ The average percentage of C-14 acetate incorporation into panaquilins B and C was higher $(1.40\%\;and\;1.13\%,$ respectively) than that into panaquilin C, (d), G-1 and G-2 $(0.75\%,\;0.65\%,\;0.13\%\;and\;0.53\%,$ respectively). Panaquilin synthesis may be depending upon the part collection period and age of the plant. The average percentage of C-14 acetate incorporation into panaquilin B is high in roots $(0.58\%)\;and\;stems\;(0.48\%);$ that into panaquilins C and (d) high in leaves $(0.40\%\;and\;0.45\%,$ respectively); and that into panaquilin E high in roots and leaves $(0.55\%\and\;0.50\%,$ respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-l). Panaquilins B, C and G-1 may be biosynthesized more actively in four-year-old plants and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-l may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and callus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that C-14 acetate was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act., 0.56 $m{\mu}Ci/mg$) and four-year-old plants (sp. act., 0.54 $m{\mu}Ci/mg$).

• 생명과학회지
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• 제14권4호
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• pp.657-663
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• 2004

고추에서 클로닝된 sesquiterpene cyclase (CASC) promoter에 cinnamic acid 4-hydroxylase (C4H)유전자를 클로닝하여 담배에 형질전환하였다. 형질전환체의 분석은 PCR, Southern blot분석으로 하였으며, CASC promoter의 작동여부를 조사하기 위하여 GUS histochemical assay를 하였다. 스트레스에 반응한다고 알려진 CASC promoter를 C4H gene과 fusion하여 C4H activity를 조사하여 스트레스에 반응하는 정도를 조사하였다. 최종적인 형질전환체로 확인된 개체는 평균 16.4% 정도였으며, 각 promoter별로 약간의 편차를 보였다. C4H activity 조사시 스트레스를 주지 않았을 때 대조구가 비슷한 양상을 나타내었다. 스트레스를 주었을 때 대조구와 비교하여 promoter 3번은 1.3배 activity가 높아졌으며 4번은 1.4배, 6은 2배, cyc 600은 1.1배 높아졌다. 이런 결과로 보아 이 promoter들은 UV에 반응하는 promoter이며 앞으로 stress에 저항하는 유전자의 적당한 promoter로, 타작물에 적용하여 stress저항성 작물의 육성 시 매우 유용하게 사용될 것으로 사료된다.

• Journal of Ginseng Research
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• 제14권2호
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• pp.291-296
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• 1990

Various rates of 2,4-D were sprayed on 2 and 3 year old ginseng plants as foliar spray to define the critical concentration. No apparent plant injury was noticable for those ginseng plants when application concentration of 2,4-D doubled the recommended dosage (70 ml/10a). Neither abnormal foliar change occurred nor any inhibition in leaf and stem growth was resulted for the plants treated with 2,4-D concentrated two times of the recommended dosage. When the rates of 2,4-D application were increased greater than this level, injury ratings increased linearly with the rates of 2,4-D application and plant you was inhibited. Ethylene gas was not produced from the ginseng plant treated with 2 times concentrated 2,4-D, however the ginseng plants produced 0.03 to 0.09 ppm ethylene gas when the rate of application were increased 3 and 4 times, respectively. On the other hand the soybean treated with the recommended amount of 21-D produced ethylene gas of 10-20 times higher compared with ginseng plants and died. Photosynthesis ability of the ginseng leaf was significantly decreased by 2,4-D foliar application but it was recovered 4 weeks after 2,4-D foliar treatment. The herbicide 2,4-D was applicated to 2,3 and 4 years old ginseng plants as foliar spray with the rates of 0.5, 1.0, 1.5 and 2.0 times of the recommended dosage to define the effects of 2,4-D on the plant growth and root yield of the ginseng. There were no significant differences in the leaf and stem growth between untreated and 2,4-D treated plant. Berry maturing of 3 and 4 year old ginseng was not influenced by 2,4-D. The root weight of 4 years old ginseng plant was not reduced by application of 2,4-D concenrated 2 times of the recommended dosage, Application time of the herbicide 2,4-D had no effects on the leaf or stem growth of 2,3 and 4 year old year old ginseng plants. When the ginseng seedling was treated with 2,4-D, detrimental phenomena as stem bending and docoration of seedling leaf margin occurred, but stem bending was recovered in a few day s. Keywords Panax ginseng C.A. Meyer, 2,4-D , herbicide.

• 한국유가공학회:학술대회논문집
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• 한국유가공기술과학회 2002년도 정기총회 및 제55회 추계심포지움 - 전환기 유가공 산업의 생존전략
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• pp.23-40
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• 2002

This study was conducted to investigate the quality changes of the UHT(ultra-high temperature), LTLT(law temperature long time) and HTST(high temperature short time) treated milk samples by storage conditions for 6 months from August 2000 to February 2001. The UHT treated milk samples collected from 3 plants(A, B and C) were stored at l0$^{\circ}$C and room temperature(dark and light exposure) for 6 months, and the LTLT and HTST treated milk samples(D and E) were also stored for 30 days. The UHT pasteurized milk of A, B and C plant was treated at 130$^{\circ}$C for 2-3s, 133$^{\circ}$C for 2-3s and 135$^{\circ}$C for 4s, respectively. The UHT sterilized milk of A and B plant was treated at 140$^{\circ}$C for 2-3s and 145$^{\circ}$C for 3-4s, respectively. The LTLT milk of D plant was treated at 63$^{\circ}$C for 30 mins, and the HTST milk of E plant was treated at 72$^{\circ}$C for 15s. All of the raw milk samples collected from storage tank in 5 milk plants were showed less than 4.0 X 10$^5$cfu/ml in standard plate count, and normal level in acidity, specific gravity, and component of milk. Preservatives, antibiotics, sulfonamides and available chloride were not detected in both raw and heat treated milk samples obtained from 5 plants. One(10%) of 10 UHT pasteurized milk samples obtained from B plant and 2 (20%) of 10 from C were not detected in bacterial count after storage at 37$^{\circ}$C for 14 days, but all of the 10 milk samples from A were detected. No coliforms were detected in all samples tested. No bacteria were also detected in carton, polyethylene and tetra packs collected from the milk plants. A total of 300 UHT pasteurized milk samples collected from 3 plants were stored at room(3$^{\circ}$C ${\sim}$ 30$^{\circ}$C) for 3 and 6 months, 11.3%(34/300) were kept normal in sensory test, and 10.7%(32/300)were negative in bacterial count. The UHT pasteurized milk from A deteriorated faster than the UHT pasteurized milk from B and C. The bacterial counts in the UHT pasteurized milk samples stored at 10$^{\circ}$C were kept less than standard limit(2 ${\times}$ 10$^4$ cfu/ml) of bacteria for 5 days, and bacterial counts in some milk samples were a slightly increased more than the standard limit as time elapsed for 6 months. When the milk samples were stored at room(3$^{\circ}$C ${\sim}$ 30$^{\circ}$C), the bacterial counts in most of the milk samples from A plant were more than the standard limit after 3 days of storage, but in the 20%${\sim}$30%(4${\sim}$6/20) of the milk samples from B and C were less than the standard limit after 6 months of storage. The bacterial counts in the LTLT and HTST pasteurized milk samples were about 4.0 ${\times}$ 10$^3$ and 1.5 ${\times}$ 101CFU/ml at the production day, respectively. The bacterial counts in the samples were rapidly increased to more than 10$^7$ CFU/ml at room temperature(12$^{\circ}$C ${\sim}$ 30$^{\circ}$C) for 3 days, but were kept less than 2 ${\times}$ 10$^3$ CFU/ml at refrigerator(l0$^{\circ}$C) for 7 days of storage. The sensory quality and acidity of pasteurized milk were gradually changed in proportion to bacterial counts during storage at room temperature and 10$^{\circ}$C for 30 days or 6 months. The standard limit of bacteria in whole market milk was more sensitive than those of sensory and chemical test as standards to determine the unaccepted milk. No significant correlation was found in keeping quality of the milk samples between dark and light exposure at room for 30 days or 6 months. The compositions of fat, solids not fat, protein and lactose in milk samples were not significantly changed according to the storage conditions and time for 30 days or 6 months. The UHT sterilized milk samples(A plant ; 20 samples, B plant ; 110 samples) collected from 2 plants were not changed sensory, chemical and microbiological quality by storage conditions for 6 months, but only one sample from B was detected the bacteria after 60 days of storage. The shelflife of UHT pasteurized milk in this study was a little longer than that reported by previous surveys. Although the shelflife of UHT pasteurized milk made a significant difference among three milk plants, the results indicated that some UHT pasteurized milk in polyethylene coated carton pack could be stored at room temperature for 6 months. The LTLT and HTST pasteurized milk should be sanitarily handled, kept and transported under refrigerated condition(below 7$^{\circ}$C) in order to supply wholesome milk to consumers.

• 한국환경복원기술학회지
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• 제21권4호
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• pp.43-63
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• 2018

The purpose of this study is to investigate and analyze the vascular plants in the Baekdudaegan Mountains and to provide the basic data necessary for future ecosystem conservation measures. The numbers of vascular plants were summarized as 699 taxa including 98 families, 338 genera, 598 species, 4 subspecies, 80 varieties, and 17 forms. There were 427 taxa in A-section, 451 taxa in B-section, 361 taxa in C-section, and 455 taxa in D-section. The rare plants were 37 taxa including 2 taxa of the CR, EN, and DD each, 12 taxa of the VU, and 19 taxa of the LC. The Korean endemic plants were 30 taxa including Salix koriyanagi Kimura, Hanabusaya asiatica (Nakai) Nakai, Carex okamotoi Ohwi, and so forth. The northern plants were 41 taxa including Equisetum hyemale L., Anemone reflexa Steph. et Willd., Symplocarpus renifolius Schott ex Miq., and so forth. The naturalized plants were 33 taxa including Fallopia dumetorum (L.) Holub, Coreopsis lanceolata L., Poa pratensis L., and so forth. The invasive alien plants were 3 taxa including Rumex acetocella L., Ambrosia artemisiifolia L., and Aster pilosus Willd.

• 한국연초학회지
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• 제23권2호
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• pp.123-132
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• 2001

In order to transform pokeweed antiviral protein cDNA to tobacco plant, total RNA was extracted from Phytolacca americana. PAP-II cDNA was synthesized from purified total RNA via RT-PCR and subcloned to recombinant vector pBluescript II SK-. 10 deletion mutant PAP-II cDNA fragments which were sequentially deleted from N-terminal by 90bp were synthesized from PAP-II cDNA except leading frame by PCR with primers designed in our laboratory. To select non-cytotoxic clone, pAc55M was constructed with yeast expression vector pAc55 and multicloning site(MCS). Sequentially deleted mutant PAP-II cDNAs were cloned on downstream of gall promoter of pAc55M. 6 non-cytotoxic deletion mutant PAP-II cDNA were selected. Selected cDNAs were cloned into plant expression vector pKGT101BH for transformation of these clones to plant through Agrobacterium tumefacience. After cloning, recombinant pKGT101BH carrying deleted mutant PAP-IIcDNA were transformed to Nicotiana tabacum cv. NC567. Transformed tobacco plants cultured on shooting and rooting media were transfered to green-house. About four weeks later, these plants were infected with physically infection using carborandum with PVY-VN strain. After 4 weeks, plants resistant to virus were selected , and seeds of these plants were gathered. Southern blot hybridization showed deleted fragments by 220bp and 420bp, so resistant ability of these plants is due to mutant PAP-II cDNA.

• 한국작물학회지
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• 제38권2호
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• pp.151-158
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• 1993

참당귀(Angelica gigas Nakai)의 재배에서 크게 문제가 되는 화성억제를 위해 온도, 일장 및 광도가 화아분화와 추대에 미치는 영향을 조사했으며, 관행재배와 화성을 추제시켜 재배생산된 근의 수량을 조사하여 연근별로 비교하였다. 본 시험에서 얻어진 결과를 요약하면 다음과 같다. 1. 자연온도처리(대조구)의 경우 11월 20일 처리구의 본엽 6～8매부터 화아분화 및 추대가 시작되어 처리기간이 길어질수록 추대율이 증가하다가 익년 1월 11일 이후부터는 변화가 없었으며 본엽 2～3매 는 어떤 구에서도 추대가 전혀 없었다. 2. 인공온도처리의 경우 처리온도가 높아질수록 주온감응한계엽령이 증가하였다. 즉, 1$^{\circ}C$, 5$^{\circ}C$, 9$^{\circ}C$ 처리구 각각 3매, 5매, 6매가지는 전혀 추대가 없었으며 8주 8매구에서도 1$^{\circ}C$에서 80%, 5$^{\circ}C$에서 45%, 9$^{\circ}C$에서 5%로 추대율이 저하하였다. 3. 참당귀의 추대율은 일장이 길어질수록 증가하였고, 광도시험에 있어서는 자연광구에서 가장 높았으나 광도가 감소할수록 점차 줄었으나 차광 50%까지 유의성이 인정되지 않았다. 4. 화도를 억제시켜 재배했을 때 10a당 건근중은 3년근이 가장 많았고 1ㆍ2년근에 비해 각각 3.6배, 2.4배 증수되었으며, 관행재배보다 2년근은 73.7%, 3년근은 159.6% 증수효과가 있었다.