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http://dx.doi.org/10.5352/JLS.2004.14.4.657

Expression of Cinnamic Acid 4-Hydroxylase Chimeric Gene fused with Sesquiterpene Cyclase Promoter from Hot Pepper in Tobacco  

이경민 (경북대학교 농업생명과학대학 식물생명과학부)
윤용휘 (경북대학교 농업생명과학대학 식물생명과학)
김길웅 (경북대학교 농업생명과학대학 식물생명과학)
이인중 (경북대학교 농업생명과학대학 식물생명과학)
신동현 (경북대학교 농업생명과학대학 식물생명과학부)
Publication Information
Journal of Life Science / v.14, no.4, 2004 , pp. 657-663 More about this Journal
Abstract
Tobacco transformants harboring cinnamic acid 4-hydroxylase gene (C4H) fused with susquiterpene cyclase promoter was developed in order to regulate biosynthesis of phenolic compounds by the expression of the introduced gene. Twenty transformants for each specific promoter were used to analyze the incorporation of the chimeric genes by PCR and Southern blot analysis. PCR products of NPTII(neomycin phosphotransferase) gene (553bp) were detected in the transgenic tobacco plants. The incorporation of the chimeric gene was confirmed in the Southern blot analysis. C4H activity in the transgenic plants was elevated by UV-irradiation and its level was higher compared to that of control plants.
Keywords
cinnamic acid 4-hydroxylase; cinnamic acid 4-hydroxylase activity; susquiterpene cyclase promoter; transgenic tobacco;
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