• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.17-17
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• 2019

진퍼리사초는 한반도 중부 이북에 분포하고, 군락 자생지는 강원도 동해안 석호 주변 모래땅 1곳인 것으로 보고되고 있다. 따라서 본 연구는 진퍼리사초 현지외보전을 위한 대량증식 연구의 일환으로 가장 효과적인 파종 시기를 구명하고자 수행하였다. 종자의 최적 발아조건을 찾기 위해 배양상 온도조건을 항온(30, 25, 20, 15, $5^{\circ}C$)과 변온(15/30, 20/10, $25/15^{\circ}C$) 총 8가지 조건으로 설정하여 발아율을 매일 측정하였다. 광조건은 명/암을 매일 12시간씩 반복하였으며 모든 실험은 국립백두대간수목원 발아실험실의 생장상에서 진행하였다. 실험결과, 항온조건에서 발아율은 $30^{\circ}C$에서 $39{\pm}7.5%$로 가장 높게 나타났고, $20^{\circ}C$ ($28{\pm}4.3%$), $25^{\circ}C$ ($24{\pm}3.7%$) 순으로 나타났으며, $15^{\circ}C$, $5^{\circ}C$에서는 발아하지 않았다. 한편, 변온조건에서의 발아율은 $25/15^{\circ}C$에서 $60{\pm}5.9%$로 가장 높게 나타났고, $15/30^{\circ}C$ ($43{\pm}4.1%$), $20/10^{\circ}C$ ($26{\pm}3.8%$) 순으로 나타났다. 따라서 진퍼리사초의 종자 발아는 항온보다 변온에서 잘 이루어졌으며, $25^{\circ}C$이상의 온도가 효과적인 것으로 나타났다. 이러한 결과를 바탕으로 가장 효과적인 파종 시기를 추정할 때, 낮 기온이 평균 $25^{\circ}C$이상이며, 일교차가 $10^{\circ}C$ 정도인 초여름이 가장 적절할 것으로 여겨진다.

• The Plant Pathology Journal
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• 제25권1호
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• pp.62-69
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• 2009

In this study, we characterized the bacterial strain KJ2C12 in relation with its biocontrol activity against Phytophthora capsici on pepper, and identified this strain using morphological, physiological, biochemical, fatty acid methyl ester, and 16S rRNA gene sequence analyses. Strain KJ2C12 significantly (P=0.05) reduced both final disease severity and areas under the disease progress curves of 5-week-old pepper plants inoculated with P. capsici compared to buffer-treated controls. As for the production of antibiotics, biofilms, biosurfactant, extracellular enzyme, HCN, and swarming activity, strain KJ2C12 produced an extracellular enzyme with protease activity, but no other productions or swarming activity. However, Escherichia coli produced weak biofilm only. Strain KJ2C12 could colonize pepper roots more effectively in a gnotobiotic system using sterile quartz sand compared to E. coli over 4 weeks after treatments. However, no bacterial populations were detected in 10 mM $MgSO_4$ buffer-treated controls. Strain KJ2C12 produced significantly higher microbial activity than the $MgSO_4$-treated control or E. coli over 4 weeks after treatments. Bacterial strain KJ2C12 was identified as Bacillus luciferensis based on morphological, physiological, and biochemical characteristics as well as FAME and 16S rRNA gene sequence analyses. In addition, these results suggested that B. luciferensis strain KJ2C12 could reduce Phytophthora blight of pepper by protecting infection courts through enhanced effective root colonization with protease production and an increase of soil microbial activity.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.88-94
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• 2005

Plant tissue culture studies have been done for the preservation of medicinal plant resources and efficient production of pharmaceutically important secondary metabolites. Micropropagation methods for Cephaelis ipecacuanha have been established and these methods enabled much more efficient propagation of the plants than the conventional methods using seedling or layering. The C. ipecacuanha plants derived from tissue culture grew uniformly in the field and they showed higher alkaloid contents compared to the plants grown from seedlings. Hairy root cultures of C. ipecacuanha and Panax ginseng have been established by infection with Agrobacterium rhizogenes, and the production of important pharmaceuticals by these cultures have been successfully demonstrated. In the case of C. ipecacuanha, the highest alkaloid yields from the hairy roots cultured for 8 weeks were 2.75-fold cephaeline (5.5 mg) and one third emetine (0.7 mg) compared with those from the roots of one-year old plant propagated through shoot-tip culture and cultivated in a greenhouse (2.0 mg cephaeline and 2.0 mg emetine). In the case of P. ginseng, ginsenoside contents in the hairy roots optimally cultured for 4 weeks were much higher than those in the roots of 4-year old field-grown plant. Thus our medicinal plant tissue cultures demonstrate desirable properties. However, they are always exposed to danger of microbial contamination or unexpected trouble of culture facilities. Cryopreservation of plant tissue cultures is a reliable method for long-term preservation. Cryopreservation studies on these cultures are also presented.

• 한국자원식물학회지
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• 제26권6호
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• pp.701-708
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• 2013

To test the quality change of seedlings of three domestic medicinal plants raised in plug trays, a short term storage experiment was conducted. Seedlings were kept in growth chambers for two weeks at 4 or $8^{\circ}C$ temperature combined with 0 or $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. Quality of glasshouse-raised seedlings was assessed after two weeks of cold storage in the growth chamber and one week of acclimation in the greenhouse. After two weeks of storage in the growth chamber of Perilla frutescens var. acuta Kudo, plant height was the greatest in the treatment $8^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. Internode length of P. frutescens var. acuta Kudo was the greatest in the treatment of $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. After one week of acclimatization in a glasshouse, the growth and development, such as plant height, internode length and leaf size, were greater in the $8^{\circ}C$ combined with $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD than in the other treatments. After two weeks of storage in the growth chamber of Sophora tonkinensis, plant height increased more in the treatment of $4^{\circ}C$ than $8^{\circ}C$. After one week of acclimatization in a glasshouse, number of leaves did not change in the treatment of $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD, but it increased in the other treatments. Leaf width increased more under the dark than light condition. Leaf length did not observably change in any treatments. After two weeks of storage in the growth chamber, plant height of Angelica gigas Nakai was the greatest in the treatment of $8^{\circ}C$. Number of leaves was the greatest in the treatment of $8^{\circ}C$. Leaf growth was greater under dark than light condition. These results suggested that optimal storage environment was $8^{\circ}C$ combined with $5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD for P. frutescens var. acuta Kudo, and $4^{\circ}C$ combined with $0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD for S. tonkinensis and A. gigas Nakai. Hence, proper combination of temperature and PPFD were necessary for better storage, and acclimatization and growth, thereafter, of the plug seedlings of theses plant species.

• 식물분류학회지
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• 제50권4호
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• pp.395-402
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• 2020

사초과의 좀목포사초(Carex brevispicula G. H. Nam & G. Y. Chung)가 한국에서 처음으로 발견되었다. 좀목포사초는 수과의 중앙부가 함몰되는 점에서 큰청사초(C. chungii Z. P. Wang) 및 목포사초(C. genkaiensis Ohwi)와 유사하다. 그러나 좀목포사초는 식물체, 웅화서와 자화서가 소형이고, 자인편이 연녹색인 특징으로 큰청사초와 구분되며, 자인편과 웅인편에 까락이 있다는 점에서 목포사초와 차이를 보인다. 새로운 국명은 목포사초보다 작다는 의미로 좀목포사초로 하였고, 주요형질에 대한 기재, 도해, 사진, 분포정보 및 근연분류군과의 차이점을 검색표로 제시하였다.

• Journal of Plant Biotechnology
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• 제35권4호
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• pp.337-343
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• 2008

We generated transgenic tomato plants with Arabidopsis thaliana $H^+$/cation exchanger gene (C4X4) by Agrobactrium-mediated transformation. We confirmed transgene copy number and transcription by Southern and Northern blot analyses. The intact CAX4-expressing tomato (Lycopersicon esculentum) fruits contained 63-71% more calcium ($Ca^{2+}$) than wild-type fruits. Moreover, ectopic expression of C4X4 in tomato fruits did not show any significant increase of the four kinds of metallic cations analyzed ($Mg^{2+}$, $Fe^{2+}$, $Mn^{2+}$, and $Cu^{2+})$. The C4X4-expressing tomato plants including their fruits did not show any morphological alternations during whole growth period. These results suggest the enhanced Ca-substrate specificity of CAX4 exchanger in tomato. Therefore, intact CAX4 exchanger can be a useful tool for $Ca^{2+}$ nutrient enrichment of tomato fruits with reduced accumulation of undesirable cations.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.18-18
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• 2019

층실사초는 경북 북부를 포함한 강원도 지역의 습기있는 음지에서 자생한다. 여러해살이풀로서 주료 사료용으로 사용되며 지피식물 등 정원소재로도 사용된다. 본 연구는 층실사초의 대량증식을 위해 가장 효과적인 파종 시기를 구명하고자 수행하였다. 종자의 최적 발아조건을 찾기 위해 배양상 온도조건을 항온조건(30, 25, 20, 15, $5^{\circ}C$)과 변온조건(15/30, 20/10, $25/15^{\circ}C$) 총 8가지 조건으로 설정하여 발아율을 매일 측정하였다. 광조건은 형광등을 이용한 명조건과 암조건을 매일 12시간씩 반복하였다. 실험결과, 항온조건에서 발아율은 $25^{\circ}C$에서 $36{\pm}6.3%$로 가장 높게 나타났고, $20^{\circ}C$ ($27{\pm}4.4%$), $30^{\circ}C$ ($24{\pm}1.6%$) 순으로 나타났으며, $15^{\circ}C$, $5^{\circ}C$에서는 발아하지 않았다. 한편, 변온조건에서의 발아율은 $25/15^{\circ}C$에서 $22{\pm}7.3%$, $15/30^{\circ}C$에서 $10{\pm}2.5%$ 발아하였고, $20/10^{\circ}C$에서는 발아하지 않았다. 따라서 층실사초 종자의 발아최적온도는 $25^{\circ}C$이고 변온보다 항온이 유리했으며, 이 이상의 온도에서는 오히려 발아율이 낮아지는 것으로 나타났다. 이러한 결과를 바탕으로 가장 효과적인 파종 시기를 추정할 때, 낮 기온 $25^{\circ}C$의 초여름에 실시하되, 밤 기온이 너무 내려가지 않도록 조절해준다면 보다 높은 발아율을 기대할 수 있을 것으로 여겨진다. 그러나 본 연구결과, 최고 발아율이 $36{\pm}6.3%$로 비교적 낮기 때문에 발아율 향상을 위한 추가적인 실험이 필요할 것이다.

• The Plant Pathology Journal
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• 제26권4호
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• pp.372-379
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• 2010

Macrophomina phaseolina causing charcoal rot was isolated from sesame seeds (cvs. Giza 32 and Shandawel-3) collected from different localities of Assiut, Sohage and El-Minia Governorates. The fungus was found in the highest frequently in samples collected from Assiut Governorate followed by Sohag and finally EL Minia Governorate. The obtained isolates were different in their virulence on the tested sesame cvs. Also, they differed in their growth nature including colony color and sclerotial production. The color of colonies of the pathogen seem to be correlated with density of sclerotial formation. Aqueous extracts of Majorna, Wild chamomile, Geranium oil and Nees plants were highly toxic to tested isolates of M. phaseolina, in vitro. On the other hand the rest of the tested aqueous extracts had no effect. Under greenhouse conditions in 2005 and 2006 seasons, soaking seeds of sesame before sowing in aqueous extracts of Eucalyptus, Nerium, Ocimum and Roesmary plants decreased the disease incidence. Aqueous extracts of Eucalyptus and Ocimum were the most effective treatment. Dipping sesame seeds in hot water at $60^{\circ}C$ for 5 minutes increased seed germination of Giza 32 and Shandawel-3 cvs. followed by $55^{\circ}C$, $50^{\circ}C$ and $45^{\circ}C$, while $40^{\circ}C$ treatment resulted the lowest seed germination rate. Dipping sesame seeds in hot water at different temperature before planting decreased seed, seedling and charcoal rots. Soaking seeds in hot water at $60^{\circ}C$ increased greatly plant height and decreased seed, seedling rot and charcoal rot followed by $55^{\circ}C$ and $50^{\circ}C$, under greenhouse condition.

• 한국식물병리학회지
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• 제14권3호
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• pp.247-252
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• 1998

A series of greenhouse test was conducted to evaluate infection of Calonectria ilicicola on soybean plants of different ages at time of inoculation. Lesion length and number of perithecia were determined on cultivars Braxton, Deltapine 726, and Riverside 699 that were 10∼40 days old and 4-10 days old at time of inoculation. Quadratic and linear relationships were described between plant age at inoculation and lesion length or perithecia production in greenhouse studies. Soybean seedlings exhibited low susceptibility to C. ilicicola regardless of cultivar susceptibility. On 8- or 1-0-days-old Braxton, lesion lenght and perithecia numbers were reduced. Lesion lengths were longest on plants 30 days old whereas perithecia production was greatest on plants 20∼30 days old at time of inoculation. Differences in lesion length and perithecia production that were observed on young plants (4∼10 days old) were similar to relative levels of susceptibility in soybean cultivars in greenhouse and field tests, suggesting that reaction to C. ilicicola in soybean cultivars may be determined early in plant development.

• 식물병연구
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• 제25권4호
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• pp.205-212
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• 2019

Analysis of differentially expressed genes has assisted discovery of gene sets involved in particular biological processes. The purpose of this study was to identify genes involved in appressorium formation in the rice blast fungus Magnaporthe oryzae via analysis of cDNA-amplified fragment length polymorphisms. Amplification of appressorial and vegetative mycelial cDNAs using 28 primer combinations generated over 200 differentially expressed transcript-derived fragments (TDFs). TDFs were excised from gels, re-amplified by PCR, cloned, and sequenced. Forty-four of 52 clones analyzed corresponded to 42 genes. Quantitative real-time PCR showed that expression of 23 genes was up-regulated during appressorium formation, one of which was the MCK1 gene that had been shown to be involved in appressorium formation. This study will be providing valuable resources for identifying the genes such as pathogenicity-related genes in M. oryzae.