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http://dx.doi.org/10.5423/RPD.2019.25.4.205

Identification of Differentially Expressed Genes by cDNA-AFLP in Magnaporthe oryzae  

Chi, Myoung-Hwan (Plant Biology Division, Noble Research Institute)
Park, Sook-Young (Department of Plant Medicine, Sunchon National University)
Publication Information
Research in Plant Disease / v.25, no.4, 2019 , pp. 205-212 More about this Journal
Abstract
Analysis of differentially expressed genes has assisted discovery of gene sets involved in particular biological processes. The purpose of this study was to identify genes involved in appressorium formation in the rice blast fungus Magnaporthe oryzae via analysis of cDNA-amplified fragment length polymorphisms. Amplification of appressorial and vegetative mycelial cDNAs using 28 primer combinations generated over 200 differentially expressed transcript-derived fragments (TDFs). TDFs were excised from gels, re-amplified by PCR, cloned, and sequenced. Forty-four of 52 clones analyzed corresponded to 42 genes. Quantitative real-time PCR showed that expression of 23 genes was up-regulated during appressorium formation, one of which was the MCK1 gene that had been shown to be involved in appressorium formation. This study will be providing valuable resources for identifying the genes such as pathogenicity-related genes in M. oryzae.
Keywords
Appressorium formation; cDNA-AFLP; Gene expression; Magnaporthe oryzae; qRT-PCR;
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