• Title/Summary/Keyword: $C_{50}$

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Study on Exfoliation and Recovery of Anesthetized Yound Abalones, Haliotis discus hannai Treated with Ethy-p-aminobenzoate and Freshwater in Different Temperatures of Sea water (온도구간별 파라아미노안식향산에틸과 담수에 의한 양식산 참전복 (Haliotis discus hannai) 치패의 박리 및 마취회복에 관한 연구)

  • 최상덕;정성채;김호진;공용근;백재민;최규정
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.281-288
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    • 1997
  • We investigated the optimal concentratin of ethyl-p-aminobenzoate for the exfoliation and recovery of young abalone, Haliotis discus hannai in according to different water temperatures, for the purpose of preventing the damage of shell and muscle to ecfoliated from shelter. In the 14$^{\circ}C$ water temperature, young abalones were exfoliated after 16, 35, 35 and 35 minutes in 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 100, 60, 30 and 30 minutes, respectively. Exfoliation rate of abalone were 100% except for 50 ppm (80%) and recovery rate were 100% of all concentration. In the $18^{\circ}C$ water temperature, young abalones were exfoliated after 4, 4, 6, 8, 8 and 12 munutes in 300, 200, 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 210, 180, 90, 60, 30, 20 and 20 minutes, respectively. Exfoliation rate of abalone were 100%, and recovery rate were 100% except for 200 and 300ppm (90%). In the $24^{\circ}C$ water temperature, young abalones were exfoliated after 8, 10, 10 and 12 minutes in 150, 100, 75 and 50ppm concentration of ethyl-p-aminobenzoate, and were recovered after 70, 50, 30 and 20 minutes, respectively. Exfoliation and recovery rate of abalone were 100%. In the 18$^{\circ}C$water temperature, exfoliation rate that treated with freshwater during 20 minute were 80, 50, 30 and 5% in 100, 75, 50 and 25% of fresh water, and recovery after 60, 15, 10 and 2 minutes, respectively and recovery arate were 100% except of r 100% freshwater. In this study, we suggest the reslults that the exfoliation and recovery by ethly-p-aminobenzoate were more effected in $18^{\circ}C\;and\;24^{\circ}C$ of sea water temperature than those of $14^{\circ}C$. The optimal concentration of ethyl-p-aminobenzoate was 50ppm at those water temperature. We raised 20 individual of young abalones at water temperature of $16^{\circ}C$ in the 1$\ell$ o ftnk and checked the variatin of dissolved oxygen (DO) by respiration of abalones that treated with 75ppm of ethyl-p-aminobenzoate. Before anesthetizion, DO were 6.17~6.20mg/$\ell$ and slowly decreased. But after 60 minutes, DO decreasing were stopped in 5.42~5.46mg/$\ell$. On the other hand, the control was continuously decreased and 5.27mg/$\ell$ after 60 minutes. The heartbeats of abalones were 33~45/minute in the water temperature of $18^{\circ}C$, but that treated with 100 ppm concentration of ethyl-p-aminobenzoate during 60 minutes, was 0/minute. And heartbeats of recovered abalones from anesthetizion were 29~43/minute.

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Studies on Optimum Shading for Seedling Cultivation of Cornus controversa and C. walteri (층층나무와 말채나무 양묘(養苗)시 적정(適正) 차광율(遮光率)에 관한 연구(硏究))

  • Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.591-597
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    • 2000
  • This studies were carried out to investigate the optimum shading for seedling cultivation of Cornus controversa and C. walteri. The experiment was performed under five different shading scheme such as 100%, 50%, 30%, 10%, and 2% of the natural full sun light intensity for 7 months in the field condition. Two species showed the highest height growth under 50% of relative light intensity, and the highest diameter growth at root collar was observed both under 100% and 50% of relative light intensity. C. controversa seedlings grown under 50% of relative light intensity produced more total biomass than control seedlings, but root biomass was less in the seedlings under 50% of relative light intensity. In case of C. walteri, total biomass production of the seedlings under 50% of relative light intensity was lower than that of the seedlings under full sun light intensity, but leaves and shoot biomass was slightly high under 50% of relative light intensity. Great reductions of height growth and diameter growth at root collar, and biomass production were observed below 30% of relative light intensity in the both species. T/R ratio of the two species was highest under 30% of relative light intensity, and the chlorophyll content of the seedlings tended to be increased by reduction of relative light intensity.

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A Comparison of Sensitivity of Four Freshwater Algae to Five Pesticides (5종 농약에 대한 4종 담수조류(freshwater algae)의 감수성 비교)

  • Park, Yeon-Ki;Kim, Byung-Seok;Bae, Chul-Han;Kim, Yeon-Sik;Park, Kyung-Hoon;Lee, Jea-Bong;Shin, Jin-Sup;Hong, Soon-Sung;Lee, Kyu-Seung;Lee, Jung-Joon
    • The Korean Journal of Pesticide Science
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    • v.12 no.1
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    • pp.50-56
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    • 2008
  • The purpose of the study was to determine the effects of isoprothiolane, diazinon, butachlor, dimethametryn and molinate in Selenastrum capriconurtum, Scenedesmus subspicatus, Chlorella vufgaris and Nitzschia palea during an exposure period of 72 hours. The study was carried out in according with the OECD Guidelines for Testing of Chemicals No 201 Alga, Growth Inhibition Test. The toxicological responses of isoprothiolane to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ (Median Effective Concentration by growth rate) value, were 5.87, 9.91, $18.55 and 38.79\;mg\;L^{-1}$, respectively. Diazinone to S. capriconutum, S. subspicatus, C. vulgaris and palea expressed as in individual $ErC_{50}$ value, were 10.31, 11.44, >32 and $14.32\;mg\;L^{-1}$, respectively. Butachlor to S. capriconutum, S. subspicatus, C. vulgaris and N. plaea expressed as in individual $ErC_{50}$ value, were 0.002, 0.019, 8.67 and $4.94\;mg\;L^{-1}$, respectively. Dimethamelryn to S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.0071, 0.011, 0.0065 and $0.009\;mg\;L^{-1}$, respectively. Molinate to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.44, 1.26, 48.84 and $28.52\;mg\;L^{-1}$, respectively. The sensitivities of five pesticides were different depending on freshwater alga in the order of S. capriconutum > S. subspicatus > C. vulgaris, N. palea. Highly significant correlation (r=0.9677) based on $ErC_{50}$ were found between S. capriconutum and S. subspicatus.

Change of quality characteristics in fresh-cut 'Romaine' lettuce by heat treatment (열처리에 따른 신선편이 '로메인' 상추의 품질 특성 변화)

  • Bae, Jeong Mi;Lee, Da Uhm;Jeong, Moon Cheol;Choi, Jeong Hee
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.27-33
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    • 2016
  • This study investigated the effect of heat treatments on the quality characteristics of fresh-cut 'Romaine' lettuce by treating in hot water (45, 50, and $55^{\circ}C$) for 2 minutes. Sensory properties, respiration rate, ethylene production, microbial growth, browning index, total phenolics (TP), vitamin C, and antioxidant properties (DPPH, ABTS, and FRAP assays) of samples were evaluated after 5 days at $5^{\circ}C$. All heat treatment conditions tested in this study did not affect the change in TP after storage. Treatment at $45^{\circ}C$ enhanced respiration rate and ethylene production wheres decreased vitamin C content and antioxidant activities. There was no significant difference in browning index at $45^{\circ}C$ treatment. The rapid tissue softening occurred when treated with $55^{\circ}C$ hot water for 2 minutes. The $50^{\circ}C$ heat treatments exhibited the best quality index including texture and color, and inhibited microbial growth and browning after storage. In addition, the $50^{\circ}C$ heat treatment showed the highest vitamin C content and antioxidant activities (DPPH, ABTS, FRAP assay) after storage. Therefore, the $50^{\circ}C$ heat treatment can be used to maintain quality and antioxidant property of fresh-cut 'Romain' lettuce.

Purification and Characterization of Peroxidase Isozyme C from Mung Bean (녹두의 Peroxidase Isozyme C의 생화학적 성장)

  • Lee, Sang-Kap;Park, Woo-Churl
    • Applied Biological Chemistry
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    • v.30 no.3
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    • pp.219-226
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    • 1987
  • Peroxidase isozyme C was isolated from mung bean cotyledon and purified to homogeneity as ascertained by chromatography and polyacrylamide gel electrophoresis, and then crystallized. Purification procedures included ammonium sulfate precipitation and column chromatography on Sephadex G-75, DEAE-cellulose and DEAE-Sephadex A-50. Peroxidase isozyme C was purified about 63 fold with 5% recovery. Isozyme C showed optimal activity at pH 5.0 with o-dianisidine and at pH 6.0 with guaiacol as substrate, and the optimal temperature was $70^{\circ}C$. Molecular weight of 50,000 was estimated for the isozyme C by SDS-polyacrylamide gel electrophoresis. At $70^{\circ}C$, it took 30 min to inactivate the isozyme to 50%, and at $80^{\circ}C$, this isozyme was almost completely inactivated in 20 min. The Km value of isozyme C for o-dianisidine was 0.11mM and that for guaiacol was 60.98mM using hydrogen peroxide as cosubstrate, and the kinetic pattern showed a competitive cyanide inhibition with respect to substrate. The crystalline structure of isozyme C was rectangular in shape.

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Resistivity Analysis to Deposition Parameters of Piezoelectric Thin Film (압전 박막의 증착변수에 따른 비저항 분석)

  • Lee, Dong-Yoon
    • Proceedings of the Korea Contents Association Conference
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    • 2007.11a
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    • pp.804-806
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    • 2007
  • C-axis oriented zinc oxide thin films were deposited on Si(100) substrate by rf magnetron sputtering. The effects of deposition parameters on the crystallinity and electrical properties of ZnO films were investigated. As-deposited ZnO films showed the strong c-axis growth andexcellent crystallinity under the deposition conditions as follows ; substrate temperaturec : $200^{\circ}C$, rf power : 150W, gas ratio : $O_2/Ar=50/50$, chamber pressure : 10mTorr. The resistivity of ZnO films was significantly affected by deposition parameters. With increasing percentage of oxygen, and decreasing substrate temperature, the resistivity of ZnO films increased.

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Antitumor Activity of Health Food-C22 (건강식품 C-22의 항암 효과)

  • Jin, Wen-Yi;Yoo, Jae-Kuk;Park, Byeong-Wook;Chen, Quan-Cheng;Bae, Ki-Hwan
    • Korean Journal of Pharmacognosy
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    • v.39 no.3
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    • pp.237-240
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    • 2008
  • C-22 is one of health food made in Han Kook Shin Yak. For studying its antitumor activity, the 50% ethanol extract from C-22 was tested on BDF1 mice bearing LLC tumor cell by Teruhiro’s method. The samples were intraperitoneally injected to mice once a day with a daily dose of 50, 100, and 200 mg/kg for 14 consecutive days. Taxol was used as positive control with a dose of 50 mg/kg. As a result, the C-22's extracts dose dependently inhibited the tumor volume with the IRTV values of 28.7, 46.7, 58.9% and the IRTW values of 28.1, 46.0, 57.8% in 50, 100, and 200 mg/kg, respectively, while the Taxol showed antitumor activity with IRTV and IRTW values of 68.5 and 67.3% in 50 mg/kg. No loss of body weight was observed at any samples. This data suggested that the C-22's antitumor activity was enough to use as a cancer chemopreventive agent, and it will be used for preventing cancer as health food.

Characteristics of Rice Flours Prepared by Moisture-Heat Treatment (수분-열처리에 의해 제조한 멥쌀가루의 특성)

  • Lee Mi-Kyung;Shin Mal-Shick
    • Korean journal of food and cookery science
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    • v.22 no.2 s.92
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    • pp.147-157
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    • 2006
  • To improve the properties of non-waxy rice flours for baking, soaked-wet milled rice flour (SWRF) was adjusted moisture content (MC) to 30 and 50 % and heated at 50 and $70^{\circ}C$ in a shaking water bath for 6 and 18 hrs, respectively. Moisture-heat treated rice flours were investigated with regard to particle size distribution, and morphological, physicochemical and pasting properties for comparing dry and wet milled flours. The particle size of rice flour treated with 30% MC was distributed between $4-20{\mu}m\;and\;100-200{\mu}m$, like SWRF. The particles above $200{\mu}m$ in the flour were produced at higher heating temperature. By SEM, starch granules were found in the rice flours treated with 30% MC, who whereas aggregated starch granules were shown in the flours treated with 50% MC. Moisture-heat treatment using higher MC and heating temperature decreased the lightness and increased the yellowness of non-waxy rice flours. Water binding capacity of 30% moisture treated rice flour was similar to that of SWRF. In the same moisture treated rice flour, swelling power was higher, but solubility was lower at $50^{\circ}C$ than at $70^{\circ}C$. The initial pasting temperature by RVA increased after moisture-heat treatment. The peak viscosity of moisture-heat treated rice flour was higher for 30% moisture than that of the others. The rice flour treated with 30% MC and heated at $50^{\circ}C$ showed low setback and increased stability for retrogradation.

Effect of Mild Heat Treatments Prior to Air Dehydration of Dried Onions Quality (열풍건조 전 순한 열처리가 건조 양파의 품질에 미치는 영향)

  • Kim, Myung-Hwan;Kim, Byung-Yong
    • Korean Journal of Food Science and Technology
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    • v.22 no.5
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    • pp.539-542
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    • 1990
  • The effects of immersion temperature $(20,\;40\;and\;60^{\circ}C)$ and immersion times (6. 12 and 18 min) in a distilled water prior to air dehydration upon the browning reaction and pyruvic acid content of air dried onions to a 4.071 moisture content (wet basis) were analyzed by a response surface methodology (RSM). Those values were also predicted by using a second degree polynomial regression model. Immersion temperature had more influence to browning reaction and pyruvic acid content than immersion time in these experimental ranges. The processing conditions to minimize the browning reaction of dried onions at $50^{\circ}C$ of air temperature (O.D.=0.071) were $60^{\circ}C$ of immersion temperature and 18 min of immersion time compared to control (O.D.=0.168) of air dehydration at $50^{\circ}C$ Pyruvic acid contents of dried onions at $50^{\circ}C$ of air temperature were maximized $(39.85{\mu}mole/g\;onion\;solid)$ at $60^{\circ}C$ of immersion temperature and 12 min of immersion time compared to control $(24.08{\mu}mole/g\;onion\;solid)$ of air dehydration at $50^{\circ}C$.

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Drying characteristics and physical properties of medicinal and edible mushrooms (약용버섯과 식용버섯의 건조방법에 따른 품질특성)

  • Kim, Bo-Min;Jung, Eun-sun;Aan, Yong-hyun;Hwang, In-Wook;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.23 no.5
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    • pp.689-695
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    • 2016
  • In this study, the drying characteristics of medicinal (Ganoderma lucidum, Phellinus linteus) and edible (Pleurotus eryngii, Lentinus edodes) mushrooms were investigated. Their shrinkage ratios, Hunter's color values, and rehydration ratios were evaluated. Mushrooms were cut with rectangular (1 cm) and square ($1{\times}1cm$) shapes, and dried in the room temperature and at $50^{\circ}C$ and $70^{\circ}C$ using hot-air dryer. Initial moisture contents of edible mushrooms were higher than those of medicinal mushrooms, whereas final moisture contents were vice versa. Drying rate was the highest when drying at $70^{\circ}C$ (p<0.05). Moreover the drying rate of square slices was higher than that of rectangular slices (p<0.05). The shrinkage ratios of both medicinal mushrooms and edible mushrooms were the least when drying at $50^{\circ}C$ and $70^{\circ}C$, respectively (p<0.05). The changes of color values were less in the medicinal mushrooms dried in the room temperature than in the hot-air dried medicinal mushrooms (p<0.05). However, in case of edible mushrooms, the changes of color values were less in the hot-air dried mushrooms (p<0.05). Rehydration ratios of medicinal mushrooms dried at $50^{\circ}C$ was the highest (p<0.05). In contrast, rehydration ratios of edible mushrooms was the highest when drying in the room temperature (p<0.05). Thus, the hot-air drying at $50^{\circ}C$ would be suggested as the efficient drying method for both medicinal mushrooms and edible mushrooms.