• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.755-761
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• 2012

In this study, we examined immuno-modulatory activities of crude polysaccharides from wild ginseng adventitious roots (WGAR). The crude polysaccharide (WGAR-CP) was isolated from WGAR by hot water extraction, ethanol precipitation, and dialysis. The major constituents in WGAR-CP were neutral sugar (64.77%), and uronic acid (34.32%). WGAR-CP demonstrated anti-complementary activity dose-dependently. The immuno-modulatory effects of WGAR-CP were also analyzed by measuring nitric oxide and cytokines in the supernatants of mouse peritoneal macrophages. Mouse peritoneal macrophages stimulated with WGAR-CP produced nitric oxide and various cytokines such as interleukin (IL)-6 and IL-12 in a dose-dependent manner. In conclusion, WGAR-CP may have immuno-modulatory activities by activating a complementary system and macrophages, which produces cytokines.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.7
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• pp.1148-1155
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• 2020

Objective: The objective was to determine standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in cereal grains and various co-products fed to growing pigs. Methods: Ten feed ingredients tested were barley (9.3% CP), lupin kernels (31.1% CP), and wheat (11.3% CP) as cereal grains, and 2 sources of corn gluten feed produced in China (21.6% CP) and Korea (24.6% CP), corn gluten meal (65.3% CP), lupin hulls (11.6% CP), rice bran (14.5% CP), soybean meal (44.8% CP), and wheat bran (15.4% CP) as co-products. Ten experimental diets were formulated to contain each ingredient as a sole source of N and an N-free diet was used to correct basal endogenous losses of CP and AA. All diets also contained 0.5% Cr₂O₃ as an indigestible index. A replicated 11×6 incomplete Latin square design with 11 dietary treatments, 6 periods, and 22 animals was employed. Twenty-two barrows with an initial body weight of 64.6±4.9 kg were equipped with a T-cannula in the distal ileum. An experimental period consisted of a 4-d adaptation period and a 2-d collection period. Results: The SID of CP in the barley, lupin kernels, wheat, 2 sources of corn gluten feed, corn gluten meal, lupin hulls, rice bran, soybean meal, and wheat bran were 84.7%, 90.5%, 90.4%, 77.4%, 74.6%, 89.5%, 90.4%, 74.4%, 86.9%, and 63.4% (standard error of the mean [SEM] = 5.3, p = 0.006), respectively. The respective SID values of Lys were 75.5%, 88.4%, 83.9%, 74.7%, 62.4%, 80.3%, 83.9%, 78.5%, 88.0%, and 71.2% (SEM = 3.3, p<0.001), and the SID values of Met were 83.6%, 88.7%, 89.4%, 85.7%, 78.3%, 88.9%, 89.4%, 85.3%, 91.1%, and 77.0% (SEM = 2.4, p<0.001), respectively. Conclusion: The ileal digestibility of protein and amino acids varies among the feed ingredients fed to pigs.

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.96-104
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• 2017

To obtain the immunomodulating polysaccharide from chaga mushroom (Inonotus obliquus sclerotia, IO), crude polysac- charide fractions (IO-M-CP and IO-CP, respectively) prepared from hot-water extract (IO-W) of I. obliquus by EtOH precipitation after MeOH reflux or not. After IO-W was re-dissolved in water followed by EtOH addition in the case without MeOH reflux, EtOH mixture was fractionated into EtOH-soluble (IO-E) and crude polysaccharide (IO-CP). In the meanwhile, MeOH-soluble fraction (IO-M) was separated from IO-W after MeOH reflux. The residue was dissolved in water and was added by EtOH, and then EtOH mixture was also fractionation into EtOH-soluble (IO-M-E) and crude polysaccharide (IO-M-CP). As a result of the macrophage stimulating activity of these fractions, IO-CP and IO-M-CP showed significantly increased cell proliferation and cytokines production than IO-W. Particularly, IO-M-CP promotes the production of IL-12 more than IO-CP. In the splenocytes proliferating activity and intestinal immune system modulating activity through Peyer's patch, both of 2 crude polysaccharide fractions were significantly promoted in cell proliferation and cytokines production than IO-W, and IO-M-CP was more potent than IO-CP in IL-2 production from splenocytes and GM-CSF production ($10{\mu}g/mL$) in Peyer's patch cells. In addition, immunomodulating polysaccharide fractions (IO-M-CP and IO-CP) prepared from IO-W by EtOH precipitation with or without EtOH reflux showed no significant difference in the chemical composition and component sugar. These results suggested that MeOH reflux might exclude low-molecular weight materials from IO-W and consequently increase the immunomodulating activity of IO-M-CP. Therefore, it was confirmed that immunomodulation of polysaccharide prepared from hot-water extract of chaga mushroom was enhanced by fractionation including MeOH reflux and EtOH precipitation.

• Journal of Plant Biology
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• v.35 no.2
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• pp.165-171
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• 1992

In order to study regulation of rbcL gene expression, rbcL gene of chloroplast DNA (Cp DNA) from maize was cloned. Cp DNA was isolated from intact chloroplast and digested with BamHI. BamHI 9 fragment of Cp DNA containing rbcL gene was ligated to pUC19 and transformed into E. coli DH5a. This recombinant plasmid was named pRLYSl. pRLYSl was hybridized with a part of rbcL gene from rice and digested with restriction enzyme BamHI, HindIIl, and PstI. From these results, it was confirmed that pRLYS1 contains intact rbcL gene and orientation of BamHI 9 fragment of Cp DNA in pRLYS1 was determined.rmined.

• Journal of Animal Science and Technology
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• v.45 no.3
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• pp.369-376
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• 2003

This experiment was conducted to investigate the influence of different levels of metabolizable energy (ME) and crude protein (CP) on post-molt performance and egg quality of spent laying hens. Four hundred and thirty two, ISA Brown hens at sixty six weeks of age after molt induction were fed six experimental diets containing three ME (2,750, 2,800, 2,850 kcal/kg) with two CP (15, 17%) levels in a factorial design. The influence of ME and CP were evaluated on egg production, egg weight and feed intake throughout 24 weeks of production. Egg mass and feed conversion ratio (FCR) were measured during the experimental period. Haugh unit, yolk color and eggshell breaking strength were measured at 5%, 50%, peak, post peak and end stage of egg production. The hens fed 2800 kcal/kg diet along with 15% CP recorded the highest outcome of egg production (P<0.05). The egg weight has been shown to increase with hens receiving 2,800 kcal/kg ME. Daily egg mass tended to increase in hens fed with 15% CP and 2,800 kcal/kg ME. Feed intake decreased significantly with the level of ME and CP in the diet increased (P<0.05). Yolk color was improved significantly in eggs laid by the hens receiving 17% CP than 15% diet. It also tended to be higher in 17% CP with 2,800 or 2,850 kcal/kg ME diet treatments. Egg shell breaking strength increased with hens receiving diets of 15% CP and 2,800 kcal/kg. From this experiment it can be concluded that the ME level of 2,800 kcal/kg coupled with 15% CP could be used to achieve the higher egg production and better eggshell strength in induced molting hens.

• Communications of the Korean Mathematical Society
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• v.20 no.3
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• pp.579-588
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• 2005

In this paper we study the asymptotics for the energy density in the self-dual Chern-Simons CP(1) model. When the sequence of corresponding multivortex solutions converges to the topological limit, we show that the field configurations saturating the energy bound converges to the limit function. Also, we show that the energy density tends to be concentrated at the vortices and antivortices as the Chern-Simons coupling constant $\kappa$ goes to zero.

• The Pure and Applied Mathematics
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• v.12 no.3 s.29
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• pp.169-178
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• 2005

In this paper we study the existence and local asymptotic limit of the topological Chern-Simons vortices of the CP(1) model in $\mathbb{R}^2$. After reducing to semilinear elliptic partial differential equations, we show the existence of topological solutions using iteration and variational arguments & prove that there is a sequence of topological solutions which converges locally uniformly to a constant as the Chern­Simons coupling constant goes to zero and the convergence is exponentially fast.

• Journal of Advanced Navigation Technology
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• v.20 no.5
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• pp.433-439
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• 2016

Several low-cost global navigation satellite system (GNSS) receivers do not support general range-domain correction, and DGNSS-CP (differential GNSS) method had been suggested to solve this problem. It improves its position accuracy by projecting range-domain corrections to the position-domain and then differentiating the stand-alone position by the projected correction. To project the range-domain correction, line-of-sight vectors from the receiver to each satellite should be calculated. The line-of-sight vectors can be obtained from GNSS broadcast ephemeris data or satellite direction information, and this paper shows positioning performance for the two methods. Stand-alone positioning result provided from Septentrio PolaRx4 Pro receiver was used to show the difference. The satellite direction information can reduce the computing load for the DGNSS-CP by 1/15, even though its root mean square(RMS) of position error is bigger than that of ephemeris data by 0.1m.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.369-378
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• 2007

Three experiments with different stage of Hanwoo steers (average BW of 167kg, 355kg and 449kg, respectively) were conducted to examine the effect of different quantities of CP intake with similar DM intake on protein balance and N excretion. Twelve Hanwoo steers in each experiment were offered three diets containing three levels of protein (9, 14 and 18% on DM basis) in the concentrate formulated with using different amount of ground corn grain or corn gluten meal. There were significant effects of increasing CP intake on CP digestibility but not on DM digestibility. Despite different CP intake, apparent fecal N outputs were not significantly affected. However, amounts of N excreted into urine increased (p<0.05) with increasing CP intake. There was an obvious effect of live body weight on efficiency of body protein conversion of CP consumed in excess of animal requirement for maintenance, with slopes of 70% and 46~39% for growing and fattening stage, respectively. Nitrogen excretion into feces and urine in this experiment was 70% of the daily N consumption for fattening stage, compared with 60% for growing stage.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.533-540
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• 2006

This experiment was conducted to determine crude protein requirements for maintenance (CPm) of growing Hanwoo steers. Eighteen Hanwoo steers (173.7kg) were allocated randomly to diets with three levels of crude protein in the concentrate by replacing ground corn with corn gluten meal: 0% (LCP), 7.2% (MCP) and 14.2% (HCP), respectively. Hanwoo steers in each treatment were fed diets containing rice straw (20%) and concentrates (80%). CP intake was 5.5, 7.8 and 10.7g/BW0.75 for LCP, MCP and HCP, respectively. Protein balance was －0.05, 2.36 and 4.07g/BW0.75 for LCP, MCP and HCP, respectively. Intercept of the regression equation between CP intake and retained CP indicated that CPm was 5.56g/BW0.75. The estimate of CPm adopted by Korean Feeding Standard (2002) was almost 20% higher than that adopted by Japanese Feeding Standard for Beef Cattle (2000); this would need to be verified with more feeding trial data set including protein requirements for growth.