• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.2
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• pp.123-134
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• 1997

The effects of cutting 6equency and nitrogen fertilization on feed values of reed canarygrass were investigated in uncultivated rice paddy and the attemp was made to estimate the economical and efficient levels of nitrogen fertilization for the increased production of nutrients in reed canarygrass. The results are summarized as follows; 1. Crude protein(CP) content was higher at 1st cut of all treatments(12.5% in 3 cutting 6equency; 19, 7% in 4 cutting frequency; 22.5% in 5 cutting kquency). However, Total Digestible Nutrients(TDN) and Relative Feed Value(RFV) in 3 and 4 cutting 6equencies were higher at 3rd cut(63.5%, 126.8) and 4th cut(65.7%, 146.4) respectively in which the contents of Neutral Detergent Fiber(NDF) and Acid Detergent Fiber(ADF) were lowest. In 5 cutting frequency, 1st cut showed the highest TDN and RFV as 70.5% and 232.4 respectively. 2. The level of nitrogen fertilization affected CP content of reed canarygrass. Nitrogen fertilization of 120kgl halcut showed high CP contents of 11.4, 14.7 and 16.5% in 3, 4 and 5 cutting eequency respectively. In 3 and 4 cutting frequency, TDN and RFV were highest with nitrogen fertilization of 90kg/ha/cut where NDF and ADF were lowest, however those in 5 cutting frequency were highest with nitrogen fertilization of 30kg/ha/cut. 3. The yields of CP varied according to the number of cutting frequency and those were 0.45~0.65 in 3 cutting hquency, 0.35~0.50 in 4 cutting frequency, and 0.19~0.67tond/ha in 5 cutting 6equency respectively and TDN was 2.09~3.60, 1.64~2.77 and 0.73~2.96tons/ha respectively. 4. The ratios between the yields of CP and TDN were 5.48, 4.60 and 4.34 in 3, 4 and 5 cutting 6equency respectively and it indicates lower ratio in result of higher number of cutting 6equency. 5. Higher nitrogen fertilization increased the yields of CP and TDN. The yields of CP were higher in 5 cutting frequency as 1.55~2.62tons/ha than of 3(1.20~1.91tons/ha) and 4 cutting frequency(1.35-2.23tons/ha). The yields of TDN were 6.31~9.93, 5.99~9.99 and 7.16~10.57tons/ha in 3, 4 and 5 cutting frequency respectively. 6. The levels of nitrogen fertilization for high production efficiency of crude protein(kg CPYkg N) was estimated as 113.4~302.5kg/ha and it resulted in 1.44~2.09ton/ha of CP yields. The levels of nitrogen fertilization for high production efficiency of dry matter was 199.2-361.3kgha in which nitrogen were fertilized below 400kg/ha and it resulted in 1.64~2.17ton/ha of CP yields. 7. The levels of nitrogen fertilization for high production efficiency of total digestible nutrients(kg TDNYkg N) were 149.9~294.1kg/ha in 3 and 4 cutting kequency and it resulted in 8.66~8.89ton/ha of TDN yileds. With the levels of nitrogen fertilization for high production efficiency of dry matter of 199.2~361.3kg/ha, TDN yields were high as 8.77~9.94ton/ha.

• The Journal of Korean Obstetrics and Gynecology
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• v.32 no.4
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• pp.1-13
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• 2019

Objective: This study is aimed to investigate the anti-tumor metastasis by innate immunomodulating effects of crude polysaccharide isolated from Polygonati Rhizoma (CP-PR) on 4T1 breast cancer cells. Methods: CP-PR was isolated from Polygonati Rhizoma. Antimetastatic experiments were conducted in vivo mouse model by using 4T1 breast cancer cells. The cell viability of CP-PR was tested with normal spleen and 4T1 breast cancer cells. To observe the activation of macrophages with/without 4T1 breast cancer cells, production of tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$), interleukin-6 (IL-6), IL-10 and IL-12 were measured with enzyme-linked immunosorbent assay (ELISA), respectively. In addition, the lysis of YAC-1 cells and the production of granzymes were measured to observe the activation of natural killer (NK) cell. Results: Intravenous administration of CP-PR significantly inhibited metastasis of 4T1 breast cancer cells. In an in vitro cytotoxicity analysis, CP-PR affected the growth of normal spleen and 4T1 breast cancer cells above specific concentration. The production of $TNF-{\alpha}$, IL-6, IL-10 and IL-12 were significantly increased in macrophages with CP-PR. As compared with control, CP-PR showed significantly higher production of $TNF-{\alpha}$, IL-10 and IL-12 in macrophages co-cultured with 4T1 breast cancer cells. The lysis of YAC-1 cells and the production of granzymes were significantly up regulated by CP-PR. Conclusion: CP-PR appears to have considerable activity on the anti-metastasis by activation of innate immune system.

• The Journal of the Korea Contents Association
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• v.16 no.9
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• pp.649-657
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• 2016

This study is aim to investigate how applying a critical pathway(CP) to stomach cancer patients affects their recovery and treatment. The subjects were 165 patients over the age of 20 who were diagnosed with stomach cancer at the gastrointestinal clinic of C hospital and who underwent laparoscopic subtotal gastrectomy. The case group inclueded 102 patients who underwent laparoscopic subtotal gastrectomy with applying the CP from October 2008 to September 2009. The control group included 63 patients who underwent laparoscopic subtotal gastrectomy without applying the CP from September 2007 to September 2008. Pain at the time of discharge was significantly lower in the CP applicated group than in the non-applicated group (Odds ratio [OR], 0.07; 95% Confidence interval [CI], 0.03-0.21). The CP applied group was significantly lower than non-applied group in total hospitalization days (CP applied group: 8.95[1.33], non-applied group: 10.69[4.04], p<0.001) and in postoperative hospitalization days (CP applied group: 7.16[0.94], non-applied group: 8.79[3.90], p<0.001). In conclusion, application of a critical pathway to laparoscopic subtotal gastrectomy reduced pain at the time of discharge, total hospitalization days and postoperative hospitalization days.

• The Journal of the Korean Society for Microbiology
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• v.15 no.1
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• pp.55-62
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• 1980

Eleven strains of lactobacilli were isolated from fermented milk of 9 companies. They were classified 3 strains as L. bulgaricus, 2 strains as L. plantarum, 2 strains as L. cellobiosus, 1 strain as L. lactis, 1 strain as L. acidophilus, 1 strain as L. casei subsp. casei and 1 strain as L. casei subsp. tolerans. And these strains were examined for drug resistance, transferability and transfer frequency of R plasmid. Most of isolates were sensitive to tetracycline(TC), penicillin(PC), and erythromycin(EM), but some strains were resistant to streptomycin(SM), chloramphenicol(CP), ampicillin(AP), kanamycin(KM), and nalidixic acid(NA). All of isolates were resistant to two or more drugs and 6 different drug resistant patterns were found. The most frequently encountered patterns were NA AP CP SM KM(5 strains) followed by NA AP CP KM(2 strains), NA AP CP SM(1 strain), NA AP CP(1 strain), NA CP(1 strain) and NA AP(1 strain). Tranfer experiment of drug resistance showed that of all 11 resistant strains, 9 strains transferred R plasmid determining AP(6 strains) or AP SM(3 strains) to a recipient, E. coli ML 1410 strain with $2.8{\times}10^{-5}-1.5{\times}10^{-1}%$ of transfer frequency. These results indicate that lactobacilli conjugally transfer their resistance to E. coli.

• The Korean Journal of Food And Nutrition
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• v.10 no.4
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• pp.497-502
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• 1997

For the purpose of enzymatic production of galactooligosaccharides from soybean arabinogalactan (SAG) hydrolysis, the $\beta$-1, 4-D-arabinogalactanase($\beta$-1, 4-galactanase) from Bacillus sp. HJ-12 was used. The soybean galactooligosaccharides(SOS) were optimally produced in SAG 1%(w/v), pH 8.0, 5$0^{\circ}C$, $\beta$-1, 4-galactanase 20unit/g SAG and 24-40 hour reaction conditions. The produced galactooligosaccharides had visocity of 11,000 cp at 75%(w/v), $25^{\circ}C$. The viscosity of galactooligosaccharides was 80 fold increasing value than that of sucrose solution. Temperature dependence of viscosity of SOS was 4.6 fold higher value than surose solution below than 5$0^{\circ}C$. Less than 50 Brix, the viscosity of SOS was similar with sucrose solution(20-40 cp), but increasing of concentration, the difference of viscosity between SOS and sucrose solution was increased. And, SOS was very stable at pH and temperature.

• Applied Chemistry for Engineering
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• v.18 no.3
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• pp.262-266
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• 2007

The effect of the presence of magnesium ions in the preparation of calcium phosphate (CP) thin films on the physicochemical and biological properties of the films has been investigated in this study. Five different surfaces were used and the culture plate (CTL) and CP film prepared in the absence of magnesium (CaP) were used for the comparison. Three different films were prepared at different magnesium concentrations. CP films prepared at the Mg concentrations of 0.1, 1, and 10 mM were designated as CaPL, CaPM, and CaPH, respectively. The observation of surface morphology of the CP films using scanning electron microscopy (SEM) displayed that the presence of magnesium affected considerably the morphology of the films including a decrease of surface porosity. X-ray diffraction (XRD) analysis for the determination of the crystallinity of the CP films showed that the structure of the films was amorphous. Examination using X-ray photoelectron spectroscopy (XPS) confirmed the prepared films consisted of calcium, phophorus, and magnesium. Cell adhesion assays elucidated that cell adhered less as the magnesium concentration increased. However, cell proliferation assays demonstrated that the cells proliferated more actively on the films prepared at the higher magnesium concentration. These results suggest that the presence of magnesium may promote the biocompatibility of CP films.

• The Korean Journal of Physiology
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• v.23 no.1
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• pp.109-117
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• 1989

The changes in adenosine triphosphate (ATP), creatine phosphate (CP) and lactic acid (LA) contents of guinea pig hearts were studied during the cardioplegia and recovery phase. 1) ATP and CP contents in cardiac ventricular tissue were decreased during the cardioplegia, regardless of $Ca^{2+}$ concentration in the cardioplegic solutions, and CP contents were recovered with the reperfusion of normal Tyrode solution faster than those of ATP. And there were no significant differences in the recovery of CP contents with different concentration of $Ca^{2+}$ in the cardioplegic solutions tested, while the recovery of ATP contents was faster with 15 mM $K^{+}$, 0.1 mM $Ca^{2+}$ cardioplegic solutions. 2) LA contents were increased during the cardioplegia and decreased with the reperfusion of normal Tyrode solution. 3) The more recovery time (up to 3 hrs), the more CP contents were recovered with the reperfusion of normal Tyrode solution faster than those of ATP. And LA contents were decreased as the duration of recovery time. These results suggest that $Ca^{2+}$ and $K^{+}$ concentration in the cardioplegic solution is one of the major factors influencing the recovery of cardiac tissue from the cardioplegia.

• Food Engineering Progress
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• v.21 no.4
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• pp.367-374
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• 2017

The aim of this study was to evaluate the quality characteristics, antioxidant activities, and sensory properties of macaron with cabbage powder (CP). Physicochemical and sensory properties of macaron with different amounts (0%, 1%, 3%, 5%, and 7%) of CP were examined. The quality of macarons was evaluated based on spread factor, baking loss rate, color, texture, antioxidant activities, and sensory evaluation. As amount of CP addition increased, spread factor, baking loss rate, and L and a values decreased, whereas b value increased (p<0.05). For texture properties, hardness, cohesiveness, and springiness were increased by addition of CP (p<0.05). The gumminess and brittleness did not show significant changes up to 3% CP addition although significant changes in 5-7% CP addition (p<0.05) were evident. The total polyphenolic contents, DPPH radical scavenging activities, and reducing power were significantly increased by addition of CP at high concentrations (p<0.05). In the consumer acceptance of macarons, 3% CP showed the highest in color, flavor, texture, and overall acceptance and 5% CP showed the highest in taste. Meanwhile, macaron containing 7% CP showed the lowest scores in texture and overall acceptance (p<0.05). These data suggested that adding 3% of CP was the optimal concentration for making macaron.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.83-92
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• 2014

This experiment was conducted to evaluate the effects of dietary CP levels in gestation under equal lysine content on reproductive performance, blood metabolites and milk composition of gilts. A total of 25 gilts ($F_1$, Yorkshire${\times}$Landrace) were allotted to 4 dietary treatments at breeding in a completely randomized design, and fed 1 of 4 experimental diets containing different CP levels (11%, 13%, 15%, or 17%) at 2.0 kg/d throughout the gestation. Body weight of gilts at 24 h postpartum tended to increase linearly (p = 0.09) as dietary CP level increased. In lactation, backfat thickness, ADFI, litter size and weaning to estrus interval (WEI) did not differ among dietary treatments. There were linear increases in litter and piglet weight at 21 d of lactation (p<0.05) and weight gain of litter (p<0.01) and piglet (p<0.05) throughout the lactation as dietary CP level increased. Plasma urea nitrogen levels of gilts in gestation and at 24 h postpartum were linearly elevated as dietary CP level increased (p<0.05). Free fatty acid (FFA) levels in plasma of gestating gilts increased as dietary CP level increased up to 15%, and then decreased with quadratic effects (15 d, p<0.01; 90 d, p<0.05), and a quadratic trend (70 d, p = 0.06). There were no differences in plasma FFA, glucose levels and milk composition in lactation. These results indicate that increasing dietary CP level under equal lysine content in gestation increases BW of gilts and litter performance but does not affect litter size and milk composition. Feeding over 13% CP diet for gestating gilts could be recommended to improve litter growth.

• Textile Coloration and Finishing
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• v.18 no.3 s.88
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• pp.49-58
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• 2006

The purpose of this study was to examine the effect of covering yarn's twist on mechanical properties of knitted fabrics of composite yarns with single covering process. Four yarns that were used in this study: two different composite yarns made from the four kinds of cotton and functional polyester-(Poly-A) with the ratio of 52:48, and the rest two yarns are the original cotton 100% yarn and the poly-A 100% yarn. The two kinds of composite yarns, CP1 and CP2, were processed on the single covering process. CP1 was applied on the single covering process with S-800 tpm, where Poly-A was used as covering yarn and cotton was used as core yarn. CP2 was applied on the same process as CP1 except that Poly-A had been applied on the two-for-one twisting process with S-400 tpm on the previous step. Sixteen mechanical properties of all the four knifed fabrics knitted under the same knitting conditions were measured by KES-FB system with the outer knit condition. And 3D CAD dressmaking simulations, which were driven by some of the mechanical properties, were presented. The results were as follows: CP2 had high RT values with twisting of covering yarn. CP2 also had high B, 2HB values because of higher linear density. SMD was affected rather by twisting of covering yarn than by fine hair of the cotton surface. Twisting of covering yarn made it decreasing T value and increasing W value. Dressmaking 3D CAD simulations showed that there are buckling effects on CP2 because of high bending rigidity and shear rigidity.