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http://dx.doi.org/10.15204/jkobgy.2019.32.4.001

Antimetastatic Effects of Crude Polysaccharide Isolated from Polygonati Rhizoma on 4T1 Breast Cancer Cells by Activation of Innate Immune System  

Ji, Hae-Ri (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Hwang, Deok-Sang (Dept. of Gynecology, College of Korean Medicine, Kyung Hee University)
Lee, Chang-Hoon (Dept. of Gynecology, College of Korean Medicine, Kyung Hee University)
Jang, Jun-Bok (Dept. of Gynecology, College of Korean Medicine, Kyung Hee University)
Lee, Jin-Moo (Dept. of Gynecology, College of Korean Medicine, Kyung Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.32, no.4, 2019 , pp. 1-13 More about this Journal
Abstract
Objective: This study is aimed to investigate the anti-tumor metastasis by innate immunomodulating effects of crude polysaccharide isolated from Polygonati Rhizoma (CP-PR) on 4T1 breast cancer cells. Methods: CP-PR was isolated from Polygonati Rhizoma. Antimetastatic experiments were conducted in vivo mouse model by using 4T1 breast cancer cells. The cell viability of CP-PR was tested with normal spleen and 4T1 breast cancer cells. To observe the activation of macrophages with/without 4T1 breast cancer cells, production of tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$), interleukin-6 (IL-6), IL-10 and IL-12 were measured with enzyme-linked immunosorbent assay (ELISA), respectively. In addition, the lysis of YAC-1 cells and the production of granzymes were measured to observe the activation of natural killer (NK) cell. Results: Intravenous administration of CP-PR significantly inhibited metastasis of 4T1 breast cancer cells. In an in vitro cytotoxicity analysis, CP-PR affected the growth of normal spleen and 4T1 breast cancer cells above specific concentration. The production of $TNF-{\alpha}$, IL-6, IL-10 and IL-12 were significantly increased in macrophages with CP-PR. As compared with control, CP-PR showed significantly higher production of $TNF-{\alpha}$, IL-10 and IL-12 in macrophages co-cultured with 4T1 breast cancer cells. The lysis of YAC-1 cells and the production of granzymes were significantly up regulated by CP-PR. Conclusion: CP-PR appears to have considerable activity on the anti-metastasis by activation of innate immune system.
Keywords
Polygonati Rhizoma; Polysaccharide; Breast Cancer; Anti-tumor Metastasis; Innate Immune System;
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Times Cited By KSCI : 4  (Citation Analysis)
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