• 청정기술
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• 제20권4호
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• pp.349-353
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• 2014

숙신산은 의약품, 식품 첨가물, 청정용매와 같은 종래의 응용 이외에도 생분해성 고분자의 중요한 전구물질로 주목 받고 있다. 그러나 최근 화석 연료의 높은 가격과 화석 원료 유래 제품의 전주기에 걸친 온실가스 배출로 인해 재생 가능한 바이오매스에 의한 숙신산의 생물학적 생산에 대한 관심이 높아지고 있다. 본 연구에서는 숙신산의 선택적인 추출과 농축을 동시에 수행할 수 있는 공정으로서 이미다졸계 이온성 액체/$K_2HPO_4$에 의한 수상이성분계를 적용하였다. 실험 결과 숙신산에 존재하는 $K_2HPO_4$ 수용액에 적정량의 이미다졸계 이온성 액체가 첨가됨에 따라 안정한 수상이성분계가 형성됨을 확인할 수 있었으며 수상이성분계 형성 능력은 [HMIm][Br]${\fallingdotseq}$[OMIm][Br]>[BMIm][Br]>[EMImBr]의 순서로 나타났다. 숙신산의 최대 추출 효율은 약 90%였으며 이온성 액체에서 양이온 사슬길이가 증가할수록 상부상으로 동반 추출되는 물의 양이 줄어들어 숙신산의 선택적 농축효과가 크게 나타났다. 따라서 본 연구에서 사용된 이미다졸계 이온성 액체와 $K_2HPO_4$으로 구성된 수상이성분계는 숙신산의 선택적 추출과 농축에 효과적임을 알 수 있었다.

• 한국산림과학회지
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• 제89권4호
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• pp.513-521
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• 2000

루브라 오리나무 (Alnus rubra Bong.) 묘목에 질소고정균, Frankia를 접종하고, 이 묘목의 반을 외생균근균, Alplva diplophloeus 포자로 접종하여 인공생장상에서 16주 동안 길렀다. 그 결과, 외생균근 형성묘는 비균근묘보다 직경생장, 칠고정뿌리혹 형성 그리고 지상부 생체량에서 6~16% 더 켰다. 이들 묘목에 대하여 30시간 동안 수분결핍 처리를 하여 균근이 루브라 오리나무의 수분생리에 미치는 영향을 조사하였다. 이 수분 결핍동안 균근은 잎의 수분 포텐셜, 광합성, 그리고 질소고정활동에 영향을 미치지 않았다. 이 결과는 외생균근균 A. diplophloeus는 수분이 부족하지 않은 경우에는 루브라오리나무 묘목의 생장을 촉진시키지만 수분결핍 상태에서는 생리활동에 영향을 미치지 않음을 나타낸다.

• Clinical Pain
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• 제20권2호
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• pp.105-121
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• 2021

Objective: Physiologically, the vastus medialis muscle is the first muscle to undergo muscle atrophy, and it was thought that pain in patients with knee osteoarthritis could be reduced if this muscle could be strengthened and stabilized. The purpose of this study was to prove the effectiveness in knee osteoarthritis using polydioxanone sutures that have been tried in other musculoskeletal areas. Method: Forty knee osteoarthritis patients voluntarily participated in the study, and divided into 30 polydioxanone suture needle (MEST-B2375 produced by Ovmedi Co.) and 10 sham needle (without suture). And the needles were inserted into the vastus medialis muscle. In all patients, safety evaluation including blood tests and ultrasonography as well as efficacy evaluation including isometric maximal contractile strength of quadriceps muscle, weight bearing pain, impression of change, quadriceps angle, rescue drug intake were evaluated up to 30 weeks after the procedure. Results: Isometric maximal contractile strength showed a significant improvement at 4 weeks after the procedure in the polydioxanone suture group, and the weight-bearing pain showed a significant improvement at every visit in the polydioxanone suture group compared with baseline values. Patient global impression of change score showed significant improvement at 20 and 30 weeks, and clinical score showed improvement at every visit. Conclusion: Insertion of polydioxanone sutures showed improvement in muscle strength and knee pain by supporting and fixation of the vastus medialis muscle in patients with degenerative knee osteoarthritis. Insertion of polydioxanone sutures is considered to have a therapeutic effect in knee osteoarthritis patients.

• 대한두개안면성형외과학회지
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• 제24권2호
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• pp.52-58
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• 2023

Background: Complex orbital fractures are impure orbital fractures because they involve the orbital walls and mid-facial bones. The author reported an orbital wall restoration technique in which the primary orbital wall fragments were restored to their prior position in complex orbital fractures in 2020. As a follow-up to a previous preliminary study, this study retrospectively reviewed the surgical results of complex orbital wall fractures over a 4-year period and compared the surgical outcomes by dividing them into groups with and without balloon restoration. Methods: Data of 939 patients with facial bone fractures between August 2018 and August 2022 were reviewed. Of these, 154 had complex orbital fractures. Among them, 44 and 110 underwent reduction with and without the balloon technique respectively. Pre- and postoperative Naugle exophthalmometer (Good-Lite Co.) scales were evaluated. The orbital volume and orbital volume ratio were calculated from preoperative and 6 months postoperative computed tomography images. Results: Among 154 patients with complex orbital fractures, 44 patients underwent restoration with the balloon technique, and 110 patients underwent restoration without it. The Naugle scale did not differ significantly between the two groups, but the orbital volume ratio significantly decreased by 3.32% and 2.39% in groups with and without the balloon technique and the difference in OVR was significantly greater in patients in the balloon restoration group compared with the control group. Postoperative balloon rupture occurred in six out of 44 cases (13.64%). None of the six patients with balloon rupture showed significant enophthalmos at 6 months of follow-up. Conclusion: The balloon rupture rate was 13.64% (6/44 cases) with marginal screw fixation, blunt screws, and extra protection with a resorbable foam dressing. Furthermore, we restored the orbital wall with primary orbital fragments using balloon support in complex orbital wall fractures.

• Applied Biological Chemistry
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• 제24권3호
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• pp.174-180
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• 1981

농약(農藥)의 사용량(使用量)이 급격(急激)히 증가(增加)함에 따라 농약(農藥)이 자연환경(自然環境)에 미치는 영향(影響)을 살펴보고 농약(農藥)의 안전성(安定性) 평가(評價)에 관(關)한 연구방법(硏究方法)을 정립(鼎立)하기 위하여 우리나라에서 많이 쓰이는 수도용(水稻用) 농약중(農藥中) 살균제(殺菌劑)인 후치왕(Fujione, Isoprothiolane), 살충제(殺蟲劑)인 오트란(Ortran, Acephate) 및 제다제(除茶劑)인 마세트(Machete Butachlor)를 대상(對象)으로 이들 농약(農藥)이 우리나라 논 토양(土壤) 미생물(微生物)에 미치는 영향(影響)에 대(對)하여 살펴 보았다. 토양(土壤) 미생물(徵生物) 수(數)의 변화(變化)를 측정(測定)하였고 미생물(微生物) 활성(活性)에 대(對)한 실험(實驗)으로 $CO_2$생성량(生成量), 질소고정능력(窒素固定能力), 질산화작용(窒酸化作用), 탈수소효소(脫水素酵素)의 역가(力價) 등을 측정(測定)한 결과(結果) 농약(農藥) 사용중(使用中) 부주의(不注意)에 의(依)한 사고(事故)를 제외(除外)하고 일반적인(一般的)인 농약(農藥) 사용(使用) 조건하(條件下)에서는 심각(深刻)한 영향(影響)은 없는 것으로 나타났다. 그러나 농약의 계속적 사용으로 인한 토양미생물 특(特)히 유전(遺傳)변이와 이에 의한 토양환경(土壤環境)의 변화(變化)등에 대(對)하여 지속적인 연구가 필요하다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권4호
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• pp.49-62
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• 2022

The magnesium and iron-based layered double hydroxide (Mg-Fe LDH) was synthesized by the co-precipitation process and the bead type LDH (BLDH, 5~6 mm in diameter) was manufactured by using the Mg-Fe LDH and the starch as a binder. To evaluate the feasibility of the BLDH as the As stabilizer in the soil, various experiments were performed and the As stabilization efficiency of the BLDH was compared to that of powdered type LDH (PLDH, <149 ㎛ in diameter). For the As sorption batch experiment, the As sorption efficiency of both of the PLDH and the BLDH showed higher than 99%. For the stabilization experiment with soil, the As extraction reducing efficiency of the PLDH was higher than 87%, and for the BLDH, it was higher than 80%, suggesting that the BLDH has similar the feasibility of As stabilization for the contaminated soil, compared to the PLDH. From the continuous column experiments, when more than 7% BLDH was added into the soil, the As stabilization efficiency of the column maintained at over 91% for 7 pore volume flushing (simulating about 21 months of rainfall) and slowly decreased down to 64% after that time (to 36 months) under the non-equilibrium conditions. Results suggested that more than 7% of BLDH added in As-contaminated soil could be enough to stabilize As in soil for a long time. The main As fixation mechanisms on the LDH were also identified through the X-ray fluorescence (XRF), the X-ray diffraction (XRD), and the Fourier transform infrared (FT-IR) analyses. Results showed that the LDH has enough of an external surface adsorption capacity and an anion exchange capability at the interlayer spaces. Results of SEM/EDS and BET analyses also supported that the Mg-Fe LDH used in this study has sufficient porous structures and outer surfaces to fix the As. The reduction of carbonate (CO₃^2-) and sulfate (SO₄^2-) anions in the LDH after the reaction between As and the LDH was observed through the FT-IR, the XRF, and the XRD analyses, suggesting that the exchange of some of these anions with the arsenate (H₂AsO₄^- or HAsO₄^2-) occurs at the LDH interlayers during the stabilization process in soil.

• Archives of Plastic Surgery
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• 제38권4호
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• pp.438-444
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• 2011

Purpose: Adipose-derived stromal cells (ASCs) are readily harvested from lipoaspirated tissue or subcutaneous adipose tissue fragments. The stromal vascular fraction (SVF) is a heterogeneous set of cell populations that surround and support adipose tissue, which includes the stromal cells, ASCs, that have the ability to differentiate into cells of several lineages and contains cells from the microvasculature. The mechanisms that drive the ASCs into the osteoblast lineage are still not clear, but the process has been more extensively studied in bone marrow stromal cells. The purpose of this study was to investigate the osteogenic capacity of adipose derived SVF cells and evaluate bone formation following implantation of SVF cells into the bone defect of human phalanx. Methods: Case 1 a 43-year-old male was wounded while using a press machine. After first operation, segmental bone defects of the left 3rd and 4th middle phalanx occurred. At first we injected the SVF cells combined with demineralized bone matrix (DBM) to defected 4th middle phalangeal bone lesion. We used P (L/DL)LA [Poly (70L-lactide-co-30DL-lactide) Co Polymer P (L/DL)LA] as a scaffold. Next, we implanted the SVF cells combined with DBM to repair left 3rd middle phalangeal bone defect in sequence. Case 2 was a 25-year-old man with crushing hand injury. Three months after the previous surgery, we implanted the SVF cells combined with DBM to restore right 3rd middle phalangeal bone defect by syringe injection. Radiographic images were taken at follow-up hospital visits and evaluated radiographically by means of computerized analysis of digital images. Results: The phalangeal bone defect was treated with autologous SVF cells isolated and applied in a single operative procedure in combination with DBM. The SVF cells were supported in place with mechanical fixation with a resorbable macroporous sheets acting as a soft tissue barrier. The radiographic appearance of the defect revealed a restoration to average bone density and stable position of pharyngeal bone. Densitometric evaluations for digital X-ray revealed improved bone densities in two cases with pharyngeal bone defects, that is, 65.2% for 4th finger of the case 1, 60.5% for 3rd finger of the case 1 and 60.1% for the case 2. Conclusion: This study demonstrated that adipose derived stromal vascular fraction cells have osteogenic potential in two clinical case studies. Thus, these reports show that cells from the SVF cells have potential in many areas of clinical cell therapy and regenerative medicine, albeit a lot of work is yet to be done.

• 한국수소및신에너지학회논문집
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• 제16권1호
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• pp.17-24
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• 2005

We experimented on growth in light and production of hydrogen and organic matters in dark fermentation by using C. reinhardtii. In the light, growth rate of C. reinhardtii following $CO_2$ fixation was proportional to consumption rate of nitrogen source. And the starch in cell was accumulated more when the period of culture was lengthened more. But the accumulation rate of starch in cell was decreased when the growth rate of cell become dull. In the dark fermentation, the production volume and production rate of hydrogen were the highest value in the mid exponential state among other states. The utilization efficiency of substrate was better in the early exponential state than other states. In production of organic matters, acetic acid didn't change remarkably and ethanol showed the highest value in early exponential state.

• KSBB Journal
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• 제20권3호
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• pp.142-148
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• 2005

이상을 통해 숙신산 분리정제를 위한 반응추출의 원리와 연구개발 및 적용 사례를 살펴보았다. 반응추출은 에너지가 적게 드는 고효율 생물분리공정이라는 장점을 확인 할 수 있었으며 향후 카르복실산 이외에 항생제를 비롯한 다양한 생물분자들의 분리정제에 적용할 수 있는 높은 응용 가능성을 갖고 있다. 그러나 특정 카르복실산의 회수를 목적으로 할 경우 추출제의 pH제한성은 극복되어야 할 문제점으로 작용할 수도 있다. 이와 병행하여 추출된 특정 카르복실산의 효과적인 회수 및 용매 재활용을 위한 재생공정의 개발도 요구된다. 기존에는 온도-스윙-재생법(temperature-swing-regeneration, TSR)이나 희석제-스윙-재생법 (diluent-swing-regeneration, DSR)을 주로 사용하였으나 회수율이 낮은 단점이 있다. 반응추출 공정의 경제성 향상을 위해서는 이를 개량하거나 대체할 수 있는 공정의 개발이 요구된다. 아울러 발효공정의 수율향상과 생성물의 동시분리를 위해 반응추출을 발효공정에 in-situ로 적용하기 위해서는 추출제 및 희석제의 생물적합성 향상을 위한 연구도 필요하다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제29권1호
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• pp.48-55
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• 2003

The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, $CO_2$, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.