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MOLECULAR IDENTIFICATION OF BACTERIA FROM OSTEOMYELITIS OF THE JAWS  

Kim, Mi-Sung (College of Dentistry, Oral Biology Research Institute, Chosun University)
Kim, Su-Gwan (College of Dentistry, Oral Biology Research Institute, Chosun University)
Chung, Hae-Man (Dept. of Oral & Maxillofacial Surgery, Dept. of Human Biology, Chosun University)
Kim, Sang-Gon (Dept. of Oral & Maxillofacial Surgery, Dept. of Human Biology, Chosun University)
Kook, Joong-Ki (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University)
Kim, Mi-Kwang (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University)
Kim, Hwa-Sook (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University)
Yoo, So-Young (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.29, no.1, 2003 , pp. 48-55 More about this Journal
Abstract
The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, $CO_2$, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.
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