• 미생물학회지
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• 제47권3호
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• pp.218-224
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• 2011

케피어그레인을 이용하여 제조한 요쿠르트로부터 젖산균 OA18을 분리하여 그 특성을 조사하였다. 분리된 균주는 그람양성, 구균이었으며, 혐기적 조건에서 이산화탄소를 생성하였다. 균주는 MRS 배지에서 $30-37^{\circ}C$ 온도 범위와 pH 7.0-9.0범위에서 잘 자랐으며, 성장을 위한 최적 온도와 pH는 각각 $37^{\circ}C$와 pH 7.0이었다. 분리된 젖산균은 리보오스, D-글루코오스, cellobiose, D-trehalose 등을 분해하여 산을 생성하였고, L-xylose, D-melibiose, inositol은 분해하지 못하였다. 16S rRNA gene 염기서열 분석을 통해 OA18 균주는 유전자은행(NCBI)에 등재되어 있는 Enterococcus faecalis (AB012212)의 염기서열과 99.8% 동질성이 있음을 확인하였다. 이와 같은 생화학적 특성과 염기서열 분석 결과를 토대로 분리된 균주를 Enterococcus faecalis OA18로 명명하였다. E. faecalis OA18균주는 오르니틴 생성능력과 Streptomyces coelicolor subsp. Flavus, S. coeruleorubidus, S. coeruleoaurantiacus, S. coelicolor, S. coeruleoprunus 대한 항균 활성을 보유하고 있었으며, 0-7% NaCl을 함유하는 MRS 배지에서 증식이 가능한 것으로 조사되었다.

• 대한교통학회지
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• 제22권4호
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• pp.97-106
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• 2004

최근에 친환경적성에 대한 사회적 관심이 높아짐에 따라 도로건설에 있어서도 주변자연 조건을 훼손을 최소화할 수 있는 터널에 대한 수요가 늘어나고 있고(2001년 12월 현재 전년대비 42.7% 증가), 터널의 연장도 장대화(일반적으로 연장 1km이상의 터널)되고 있다. 그러나 장대터널은 장시간동안 좁고 한정된 공간에서의 주행을 하게 되는 운전자의 심리적 불안감을 증가시키고 집중력과 주변지역으로부터의 외부정보 인지가 충분하지 않는 등 인적, 물적의 다양한 교통문제와 깊은 관련을 갖고 있다. 따라서 본 연구에서는 현재 개통 운영 중인 터널을 대상으로 조명색채에 따른 내부경관을 연구함으로서 터널 내부조명시설의 개선을 통한 주행 쾌적성의 증대효과를 기대할 수 있는 방안제시에 주 목적을 두고 있다. 본 연구에서는 조명색채에 대한 감성이미지를 나타내기 위한 형용사 선정을 위하여 컬러 플래닝 시스템(CPS)를 사용하였다. CPS는 인간의 감성 표현을 정의된 "용어"로 표현하고 이 "용어"는 "이미지 스케일"상에 검색되어 이에 결합된 "이미지 패널"로 특정의 색채로 지정되는 일련의 과정을 나타낸다. 그리고 이렇게 선정된 용어로 SD(Semantic Differential Scale)법을 이용하여 설문지를 작성하고, 요인분석을 통해 피실험자들의 의식구조를 파악하였다. 또한 LISREL을 이용하여 터널 내부경관의 '선호도'를 종속변수로 한 모형을 구축한 결과, 조명색채에 대한 경관선호도에 영향을 미치는 요인으로 색채의 정서성과 기능성을 제시하였다.

• 만화애니메이션 연구
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• 통권13호
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• pp.211-221
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• 2008

지상파방송사의 애니메이션 편성집중도가 방송사자체의 중장기적 수익모델인 방송사이미지에 어떠한 연관성을 잠재하고 있다면, 또한 그러한 분석결과가 연구성과로 검증된다면, 지상파방송사의 애니메이션 편성의지와 전략 또한 공격적이고 적극적인 방향으로 전환될 수 있을 것이라는 가정이 본 연구의 목적이며 출발점이다. 본 논문의 연구문제는 다음과 같다. 첫째, 기존 지상파방송사의 애니메이션 편성 및 방영비율이 시청자들의 지속적인 시청집중현상과 어떻게 연계되는가? 둘째, 방송사의 애니메이션 편성방영비율의 지속적 강화가 시청자의 방송사 채널 이미지 형성에 어떠한 영향을 미치는가? 셋째, 뉴미디어 플랫폼에 애니메이션 편성 및 방영전략을 강화해야 하는 중장기적 전략의 필요성은 무엇인가? 등이다. 본 연구에서는 1580년대의 지상파 방송사가 편성 방영했던 애니메이션 프로그램의 비율을 기준으로, 현재 방송사의 선호도 및 프로그램 시청집중도와 어떠한 상관관계가 존재할 수 있는지 분석했다. 이는 방송사의 애니메이션 프로그램에 대해 선호유무를 주관적으로 판단할 수 있는 8세부터 14세의 어린이 및 청소년들이 애니메이션을 보기 위해 선택한 채널의 집중적인 시청행위가, 20년 이후 직접적인 경제활동인구로서 주관적인 상품구매의사결정을 할 수 있게 된 20대 후반부터 30대 후반에 이르러 지상파방송사의 채널선택을 할 때(동일하거나 유사한 방송프로그램이 동 시간대에 방영될 때) 본인의 채널선호도와 어떠한 상관관계를 가질 수 있는가에 대해 분석한 것이다. 결국, 이러한 상관관계연구는 새롭게 제시되고 있는 뉴미디어 플랫폼에서 애니메이션 프로그램에 대해 어떠한 편성전략과 기획전략을 진행해야 하는가에 대한 예측변수를 제시할 수 있다. 본 논문의 분석결과에 의하면, 현재 지상파방송사가 지속적인 채널선호도를 중장기적으로 유지하기 위해서라도 직간접적인 애니메이션 제작투자를 통해 애니메이션 프로그램의 편성비율을 최소한으로 유지하는 것 이상으로 편성비율에 대해 전략적으로 접근해야 될 필요성이 있다. 또한, 초고속통신망 회사들, 혹은 다양한 통신업계 기업들도 전략적인 입장에서 중장기적인 애니메이션 제작투자 및 캐릭터개발에 집중해야 될 때이다.

• 한국미생물·생명공학회지
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• 제36권4호
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• pp.299-306
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• 2008

사람 췌장 리파아제는 췌장에서 합성되어 소장으로 분비된 후, 음식물속의 지방을 가수분해하는 소화효소이다. 췌장 리파아제 단백질은 촉매활성도메인과 코리파아제 결합도메인의 2개의 도메인으로 구성되어 있다 본 연구에서는 리파아제 전체단백질과 촉매활성도메인 및 코리파아제 결함도메인 유전자를 PCR 방법으로 증폭하여 발현벡터에 넣은 후, 대장균에서 발현시켜 얻은 3가지 재조합 단백질을 산란계에 3차례 주사하여 면역반응을 유도하였다. 난황단백질을 분리하여 항체가를 측정한 결과, 코리파아제 결합도메인의 항원성이 가장 높은 것으로 밝혀졌다. 또한, 돼지 췌장 리파아제의 활성저해 실험에서도 코리파아제 결합도메인에 의해 만들어진 난황항체가 지방분해활성 저해작용이 가장 큰 것으로 밝혀졌다. 코리파아제 결합도메인 난황항체가 첨가된 지방을 실험동물에게 급여하고 혈중 주요 성분을 분석한 결과, 코리파아제 결합도메인에 의한 난황항체를 섭취한 경우에 혈중 중성지방의 수치가 대조군에 비해서 유의적으로 감소했음을 화인하였다. 결론적으로 코리파아제 결합도메인을 항원단백질로 사용하여 얻은 난황항체가 생체 내에서 췌장 리파아제의 활성을 억제할 수 있으며 비만억제제로의 개발 가능성이 있음을 보여주었다.

• 대기
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• 제23권1호
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• pp.93-102
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• 2013

Atmospheric transport pathway of an air mass is an important constraint controlling the chemical properties of the air mass observed at a designated location. Such information could be utilized for understanding observed temporal variabilities in atmospheric concentrations of long-lived chemical compounds, of which sinks and/or sources are related particularly with natural and/or anthropogenic processes in the surface, and as well as for performing inversions to constrain the fluxes of such compounds. The Lagrangian particle dispersion model FLEXPART provides a useful tool for estimating detailed particle dispersion during atmospheric transport, a significant improvement over traditional "single-line" trajectory models that have been widely used. However, those without a modeling background seeking to create simple back-trajectory maps may find it challenging to optimize FLEXPART for their needs. In this study, we explain how to set up, operate, and optimize FLEXPART for back-trajectory analysis, and also provide automatization programs based on the open-source R language. Discussions include setting up an "AVAILABLE" file (directory of input meteorological fields stored on the computer), creating C-shell scripts for initiating FLEXPART runs and storing the output in directories designated by date, as wells as processing the FLEXPART output to create figures for a back-trajectory "footprint" (potential emission sensitivity within the boundary layer). Step by step instructions are explained for an example case of calculating back trajectories derived for Anmyeon-do, Korea for January 2011. One application is also demonstrated in interpreting observed variabilities in atmospheric $CO_2$ concentration at Anmyeon-do during this period. Back-trajectory modeling information introduced in this study should facilitate the creation and automation of most common back-trajectory calculation needs in atmospheric research.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.267-271
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• 2012

An experiment was conducted to evaluate the effects of one alpha-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) on growth performance, tibia quality, and skin and meat color in broilers fed calcium (Ca)- and phosphorus (P)-deficient corn-soybean meal diets. A total of 288 male Ross broilers, at 21 days of age, were randomly assigned to three treatments with eight cages per treatment. Three levels of $1{\alpha}$-OH $D_3$ (0, 5, and 10 ${\mu}g$/kg) were added to a basal diet (0.50% Ca, 0.13% non-phytate phosphorus (NPP), and 0.35% total phosphorus (tP)) without vitamin $D_3$. As a result of this study, the addition of $1{\alpha}$-OH $D_3$ increased body weight gain (p<0.001), feed intake (p = 0.007), feed efficiency (p<0.001), tibia weight (p = 0.002), length (p<0.001), breaking-strength (p = 0.012), ash (p<0.001), Ca (p<0.001), and P content (p = 0.004). Dietary $1{\alpha}$-OH $D_3$ enhanced breast meat yellowness (p = 0.015) and the length and weight of the small intestine of the broilers. Moreover, $1{\alpha}$-OH $D_3$ decreased serum Ca concentration (p = 0.074) and breast meat redness (p = 0.010). These results indicate that the $1{\alpha}$-OH $D_3$ improves growth, tibia quality, and meat color in broilers fed Ca- and P-deficient corn-soybean meal diets.

• Journal of Pharmaceutical Investigation
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• 제25권3호spc1호
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• pp.7-20
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• 1995

Taurine, a ${\beta}-amino$ acid, plays an important role as a neuromodulator and is necessary for the normal development of the brain. Since de novo synthesis of taurine in the brain is minimal and in vivo studies suggest that taurine dose not cross the blood-brain barrier, we examined whether the choroid plexus, the blood-cerebrospinal fluid (CSF) barrier, plays a role in taurine transport in the central nervous system. The uptake of $[^3H]-taurine$ into ATP depleted choroid plexus from rabbit was substantially greater in the presence of an inwardly directed $Na^+$ gradient taurine accumulation was negligible. A transient in side-negative potential gradient enhanced the $Na^+-driven$ uptake of taurine into the tissue slices, suggesting that the transport process is electrogenic, $Na^+-driven$ taurine uptake was saturable with an estimated $V_{max}$ of $111\;{\pm}\;20.2\;nmole/g/15\;min$ and a $K_M\;of\;99.8{\pm}29.9\;{\mu}M$. The estimated coupling ratio of $Na^+$ and taurine was $1.80\;{\pm}\;0.122.$ $Na^+-dependent$ taurine uptake was significantly inhibited by ${\beta}-amino$ acids, but not by ${\alpha}-amino$ acids, indicating that the transporter is selective for ${\beta}-amino$ acids. Since it is known that the physiological concentration of taurine in the CSF is lower than that in the plasma, the active transport system we characterized may face the brush border (i.e., CSF facing) side of the choroid plexus and actively transport taurine out of the CSF. Therefore, we examined in vivo elimination of taurine from the CSF in the rat to determine whether elimination kinetics of taurine from the CSF is consistent with the in vitro study. Using a stereotaxic device, cannulaes were placed into the lateral ventricle and the cisterna magna of the rat. Radio-labelled taurine and inulin (a marker of CSF flow) were injected into the lateral ventricle, and the concentrations of the labelled compounds in the CSF were monitored for upto 3 hrs in the cisterna magna. The apparent clearance of taurine from CSF was greater than the estimated CSF flow (p<0.005) indicating that there is a clearance process in addition to the CSF flow. Taurine distribution into the choroid plexus was at least 10 fold higher than that found in other brain areas (e. g., cerebellum, olfactory bulb and cortex). When unlabelled taurine was co-administered with radio-labelled taurine, the apparent clearance of taurine was reduced (p<0.0l), suggesting a saturable disposition of taurine from CSF. Distribution of taurine into the choroid plexus, cerebellum, olfactory bulb and cortex was similarly diminished, indicating that the saturable uptake of taurine into these tissues is responsible for the non-linear disposition. A pharmacokinetic model involving first order elimination and saturable distribution described these data adequately. The Michaelis-Menten rate constant estimated from in vivo elimination study is similar to that obtained in the in vitro uptake experiment. Collectively, our results demonstrate that taurine is transported in the choroid plexus via a $Na^+-dependent,saturable$ and apparently ${\beta}-amino$ acid selective mechanism. This process may be functionally relevant to taurine homeostasis in the brain.

• The Korean Journal of Physiology and Pharmacology
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• 제15권4호
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• pp.195-201
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• 2011

The flavonoid quercetin is a low molecular weight compound generally found in apple, gingko, tomato, onion and other red-colored fruits and vegetables. Like other flavonoids, quercetin has diverse pharmacological actions. However, relatively little is known about the influence of quercetin effects in the regulation of ligand-gated ion channels. Previously, we reported that quercetin regulates subsets of nicotinic acetylcholine receptors such as ${\alpha}3{\beta}4$, ${\alpha}7$ and ${\alpha}9{\alpha}10$. Presently, we investigated the effects of quercetin on muscle-type of nicotinic acetylcholine receptor channel activity expressed in Xenopus oocytes after injection of cRNA encoding human fetal or adult muscle-type of nicotinic acetylcholine receptor subunits. Acetylcholine treatment elicited an inward peak current ($I_{ACh}$) in oocytes expressing both muscle-type of nicotinic acetylcholine receptors and co-treatment of quercetin with acetylcholine inhibited $I_{ACh}$. Pre-treatment of quercetin further inhibited $I_{ACh}$ in oocytes expressing adult and fetal muscle-type nicotinic acetylcholine receptors. The inhibition of $I_{ACh}$ by quercetin was reversible and concentration-dependent. The $IC_{50}$ of quercetin was $18.9{\pm}1.2{\mu}M$ in oocytes expressing adult muscle-type nicotinic acetylcholine receptor. The inhibition of $I_{ACh}$ by quercetin was voltage-independent and non-competitive. These results indicate that quercetin might regulate human muscle-type nicotinic acetylcholine receptor channel activity and that quercetin-mediated regulation of muscle-type nicotinic acetylcholine receptor might be coupled to regulation of neuromuscular junction activity.

• Nutrition Research and Practice
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• 제12권5호
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• pp.378-386
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• 2018

BACKGROUND/OBJECTIVES: Benign prostatic hypertrophy (BPH) is a major cause of abnormal overgrowth of the prostate mainly in the elderly. Corni Fructus has been reported to be effective in the prevention and treatment of various diseases because of its strong antioxidant effect, but its efficacy against BPH is not yet known. This study was designed to evaluate the therapeutic efficacy of Corni Fructus water extract (CF) in testosterone-induced BPH rats. MATERIALS/METHODS: To induce BPH, rats were intraperitoneal injected with testosterone propionate (TP). Rats in the treatment group were orally administered with CF with TP injection, and finasteride, which is a selective inhibitor of $5{\alpha}$-reductase type 2, was used as a positive control. RESULTS: Our results showed that the increased prostate weight and histopathological changes in BPH model rats were suppressed by CF treatment. CF, similar to the finasteride-treated group, decreased the levels of testosterone and dihydrotestosterone by TP treatment in the serum, and it also reduced $5{\alpha}$-reductase expression and concentration in prostate tissue and serum, respectively. In addition, CF significantly blocked the expression of the androgen receptor (AR), AR co-activators, and proliferating cell nuclear antigen in BPH rats, and this blocking was associated with a decrease in prostate-specific antigen levels in serum and prostate tissue. CONCLUSIONS: These results suggest that CF may weaken the BPH status through the inactivation of at least $5{\alpha}$-reductase and AR activity and may be useful for the clinical treatment of BPH.

• 한국환경보건학회지
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• 제44권4호
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• pp.397-407
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• 2018

Objectives: The purpose of this study is to investigate human health risk assessment of indoor air pollutants at small-sized public-use facilities (e.g., daycare centers, hospital and elderly care facilities) that the susceptible population is mainly used. Methods: To assess indoor air quality (IAQ), the concentrations of indoor air contaminants such as HCHO, benzene, toluene, ethylbenzene, xylene, styrene, PM-10, CO, $NO_2$ and $O_3$ in air samples were measured according to the Indoor Air Quality Standard Method. By conducting the questionnaire survey, the major factors influencing IAQ were identified. Human health risk assessment was carried out in the consideration of type of use (user and worker) at 75 daycare centers, 34 hospitals and 40 elderly care facilities. Results: As a result of measurement of indoor air contaminants, the average concentration of HCHO and TVOCs in hospitals was higher than daycare centers and elderly care facilities, about 8.8 and 23.5% of hospitals were exceeded by IAQ standard. In human health risk assessment, for the user of daycare centers and elderly care facilities, the mean carcinogenic risk of HCHO inhalation was higher than acceptable value. Except for HCHO, other values were determined under acceptable risk. Similarly, for the worker of hospitals, the mean carcinogenic risk of HCHO inhalation was higher than acceptable value and other values were evaluated under acceptable risk. In contrast, the risk levels of other contaminants measured in elderly care facilities were acceptable. In the determination of factors influencing IAQ, the construction year, building type, ventilation time, and the use of air cleaner were identified. Conclusions: This study provides the information for establishing the plans of public health management of IAQ at small-sized public-use facilities that have not yet been placed under the regulation. The findings suggest the consideration of human health risk assessment results for the IAQ standards.