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Development of Egg Yolk Antibody Specific to the Pancreatic Lipase Domain for Anti-Obesity  

Woo, Seung-Eun (Life Sciences Research Center, DAN Biotech)
Kwon, Jin-Hyuk (Life Sciences Research Center, DAN Biotech)
Yang, Si-Yong (Life Sciences Research Center, DAN Biotech)
Park, Hyun-Ju (Department of Biotechnology, the Catholic University of Korea)
Kim, Hyung-Kwoun (Department of Biotechnology, the Catholic University of Korea)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.4, 2008 , pp. 299-306 More about this Journal
Abstract
Human pancreatic lipase is a digestive enzyme which is synthesized in pancreas, secreted into small intestine, and there hydrolyze the fat in food. Pancreatic lipase protein composes of catalytic domain and colipase-binding domain. In this research, the gene segments corresponding to total protein, catalytic domain, and co lipase-binding domain were cloned by PCR method, inserted into an expression vector, and then used to transform Escherichia coli BL21 (DE3). The recombinant proteins produced were purified and injected intramuscularly three times into laying hens. The egg yolk antibodies (IgY) were obtained from the egg yolks and tested for their antibody titer. Among three IgY, the IgY against colipase-binding domain showed the highest antibody titer. All three IgY had inhibitory effects on the porcine pancreatic lipase. Among them, the IgY against colipase-binding domain showed the highest inhibition effects. The fat diet with corn oil and IgY was administrated to the experimental rats and their blood compositions were examined with time course. The triglyceride concentration of treated rats was decrease meaningfully when compared with those of control rats. This suggested that the IgY against colipase-binding domain antigen inhibited pancreatic lipase in vivo.
Keywords
Egg yolk antibody; pancreatic lipase; colipase-binding domain; lipase inhibition; obesity;
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