• Asian Pacific Journal of Cancer Prevention
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• v.17 no.12
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• pp.5229-5235
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• 2016

Prostate cancer (PCa) remains one of the most widespread and perplexing of all human malignancies. Assessment of gene expression is thought to have an important impact on cancer diagnosis, prognosis and therapeutic decisions. In this context, we explored combined expression of PCa related target genes AMACR and PCA3 in 126 formalin fixed paraffin embedded prostate tissues (FFPE) from Moroccan patients, using quantitative real time reverse transcription-PCR (RT-qPCR). This quantification required data normalization accomplished using stably expressed reference genes (RGs). A panel of twelve RG was assessed, data being analyzed using GenEx V6 based on geNorm, NormFinder and statistical methods. Accordingly, the hnRNP A1 gene was identified and selected as the most stably expressed RG for reliable and accurate gene expression quantification in prostate tissues. The ratios of both PCA3 and AMACR gene expression relative to that of the hnRNP A1 gene were calculated and the performance of each target gene for PCa diagnosis was evaluated using receiver-operating characteristics. PCA3 and AMACR mRNA quantification based on RT-qPCR may prove useful in PCa diagnosis. Of particular interesting, combining PCA3 and AMACR quantification improved PCa prediction by increasing sensitivity with retention of good specificity.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.6-11
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• 2007

[ $\beta$ ]-Fructofuranosidases, a family 32 of glycoside hydrolases (GH32), share three conserved domains including the W(L/M)(C/N)DP(Q/N), FRDPK, and ECP(D/G) motifs. The functional role of the conserved acidic residues within three domains of levan fructotransferase, one of the $\beta-fructofuranosidases$, from Microbacterium sp. AL-210 was studied by site-directed mutagenesis. Each mutant was overexpressed in E. coli BL21(DE3) and purified by using Hi-Trap chelating affinity chromatography and fast performance liquid chromatography. Substitution of Asp-63 by Ala, Asp-195 by Asn, and Glu-245 by Ala and Asp decreased the enzyme activity by approximately 100-fold compared to the wild-type enzyme. This result indicates that three acidic residues Asp-63, Asp-195, and Glu-245 play a major role in catalysis. Since the three acidic residues are present in a conserved position in inulinase, levanase, levanfructotransferase, and invertase, they are likely to have a common functional role as nucleophile, transition state stabilizer, and general acid in $\beta-fructofuranosidases$.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.41 no.5
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• pp.508-516
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• 1992

In this study, dielectric and piezoelectric properties of 0.05Pb(AlS12/3TWS11/3T)OS13T-0.95Pb(ZrS10.52TTiS10.48T)OS13T system ceramics were investigated with respect to the variations of MnOS12T and FeS12TOS13T additions amounts. The results obtained in this study are summarized as follows: 1. As the amounts of MnOS12T and FeS12TOS13T are increased, tetragonality(c/a) and apparent density were decreased but grain size was increased, also the limits of solubility were revealed because pores were formed at the amounts of 0.3wt% MnOS12T and 0.5wt% FeS12TOS13T. 2. AS the increasing of amounts of MnOS12T and FeS12TOS13T, the temperature of phase transition(TS1cT) was decreased, and pemeability had maximum value at the amount of 0.3wt% MnOS12T but were sharply decreasd for the increasing FeS12TOS13T amounts. 3. As the amounts of MnOS12T and FeS12TOS13T are increased, the electro-mechanical coupling factor(kS1pT) was decreased from 60% to 41%, 19% respectively, but mechanical quality factor(QS1mT) had maximum values 720 for amount of 0.3wt% MnOS12T and 320 the amount of 0.5wt% FeS12TOS13T.

• Journal of the Korean Society for Heat Treatment
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• v.17 no.2
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• pp.94-100
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• 2004

In order to improve the wear resistance of Ti-6Al-4V alloy, plasma carburization treatment was newly carried out without consumption of its good specific strength and fatigue life over the temperature. Effect of the plasma carburization was analyzed and compared with the non-treated alloy by microstructural observation, structure characterization and mechanical test. The plasma treated alloy formed a carburized layer of about $150{\mu}m$ in depth from the surface, where a fine and hard particles of TiC and $V_4C_3$ were homogeneously dispersed through the layer. The steady-static creep behaviors of Ti-6Al-4V alloy, using the constant stress creep tester, were investigated over the temperature range of $510{\sim}550^{\circ}C$(0.42~0.44Tm) and the stress range of 200~275 MPa. Stress exponent(n) was decreased from 9.32 of non-treatment specimen to 8.95 of carburized, however, the activation energy(Q) increased from 238 to 250 kJ/mol with the same condition as indicated above. From the above results, it can be concluded that the static creep deformation for Ti-6Al-4V alloy was controlled by the dislocation climb over the ranges of the experimental conditions.

• Metals and materials international
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• v.24 no.6
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• pp.1376-1385
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• 2018

Ultrasonic melt treatment (UST) was applied to an A390 hypereutectic Al-Si alloy in a temperature range of $750-800^{\circ}C$ and its influence on the solidification structure and the consequent increase in strength was investigated. UST at such a high temperature, which is about $100^{\circ}C$ above the liquidus temperature, had little effect on the grain refinement but enhanced the homogeneity of the microstructure with the uniform distribution of constituent phases (e.g. primary Si, ${\alpha}-Al$ and intermetallics) significantly refined. With the microstructural homogeneity, quantitative analysis confirmed that UST was found to suppress the formation of Cu-bearing phases, i.e., $Q-Al_5Cu_2Mg_8Si_6$, $Al_2Cu$ phases that form in the final stage of solidification while notably increasing the average Cu contents in the matrix from 1.29 to 2.06 wt%. A tensile test exhibits an increase in the yield strength of the as-cast alloy from 185 to 208 MPa, which is mainly associated with the solute increment within the matrix. The important role of UST in the microstructure evolution during solidification is discussed and the mechanism covering the microstructure-strengthening interrelationship of the ultrasonically treated A390 alloy is proposed.

• Transactions of Materials Processing
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• v.7 no.5
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• pp.450-458
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• 1998

The high temperature deformation behavior of Al 5083 alloy has been studied in the temperature range of 350 to 520 ${\circ}C$ and strain rate range of 0.2 to 3.0/sec by torsion test. The strain rate sensitivity(m) of the material was evaluated and used for estabilishing power dissipation maps following the dynamic material model. These maps show the variation of efficiency of power dissipation(${\eta}$=2m/(2m+1)) with temperature and strain rate. Hot restoration of dynamic recrystallization (DRX) was analyzed from the flow curve, deformed microstructure, and processing maps during hot deformation. Also, the effect of deformation strain on the efficiency of power dissipation of the alloy was analysed using the processing maps. Moreover relationship between the hot-ductility and efficiency of power dissipation of the alloy depending on thmperature and strain rate was studied using the Zener-Hollomon parameter(Z=${\varepsilon}$exp(Q/RT) It is found that the maximum efficiency of power dissipation for DRX in Al 5083 alloy is about 74.6 pct at the strain of 0.2. The strain rate and temperature at which the efficiency peak occurred in the DRX domain is found to be ∼0.1/sec and ∼450${\circ}C$ respectively.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.61-66
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• 2003

In this paper, the effect of binder additive on the fracture toughness was metal matrix composite produced by squeeze casting method. In this study using the matrix is AC4CH and reinforcement used Aluminum borate whisker. Each MMC was produced by add inanimate binder ($SiO_2,\;Al2O_3,\;TiO_2$) to whiskers for increase the binding together of whiskers. Fracture toughness test were carry out in accordance with the ASTM E-399 standard test method, W=25mm, b=12.5mm CT(half size) specimen. However Base metal AC4CH was not meet the qualification of $P_m/P_Q<1.1$, so that test were performed $J_{IC}$ test. $K_{J_{IC}}$ value was measuring by $J_{IC}$ value change into $K_{IC}$. $J_{IC}$ test was carry out in accordance with the ASTM 1820.

• Honam Mathematical Journal
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• v.26 no.3
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• pp.247-256
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• 2004

Buchmann and Williams[1] proposed a key exchange system making use of the properties of the maximal order of an imaginary quadratic field. $H{\ddot{u}}hnlein$ et al. [6,7] also introduced a cryptosystem with trapdoor decryption in the class group of the non-maximal imaginary quadratic order with prime conductor q. Their common techniques are based on the properties of the invertible ideals of the maximal or non-maximal orders respectively. Kim and Moon [8], however, proposed a key-exchange system and a public-key encryption scheme, based on the class semigroups of imaginary quadratic non-maximal orders. In Kim and Moon[8]'s cryptosystem, a non-invertible ideal is chosen as a generator of key-exchange ststem and their secret key is some characteristic value of the ideal on the basis of Zanardo et al.[9]'s quantity for ideal equivalence. In this paper we propose the methods for finding the non-invertible ideals corresponding to non-primitive quadratic forms and clarify the structure of the class semigroup of non-maximal order as finitely disjoint union of groups with some quantities correctly. And then we correct the misconceptions of Zanardo et al.[9] and analyze Kim and Moon[8]'s cryptosystem.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.341-345
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• 1998

Soil solutions were collected by zero-tension lysimeters at Kwangju, Kyunggi Province to estimate differences in ion concentration among species and horizons. Zero-tension lysimeters were installed in O, A, and B horizons in Larix leptolepis, Pinus koraiensis, and Quercus mongolica stands. Soil solution samples were collected from September 1996 through August 1998 and analyzed for $K^+$, $NA^+$, $NH_4^+$, $Mg^{2+}$, $Ca^{2+}$, $Al^{3+}$, $Cl^-$, $SO_4^{2-}$, $NO_3^-$, and $PO_4^{3-}$. The experimental site had high nitrogen loading from the atmosphere, and $NO_3^-$ was positively correlated with $K^+$ and $Mg^{2+}$. However, $NO_3^-$ and $NH_4^+$ showed a positive correlation only in the O horizon of Q. mongolica stand. Mg^(2+) deficit in the soil was predicted owing to the positive relationship of $Mg^{2+}$ with $NO_3^-$. Concentrations of $K^+$ and $Ca^{2+}$in soil solution were estimated higher in L. leptolepis than in other stands because of high leaching from the plant. Concentration of $Al^{3+}$ in soil solution was negatively correlated with soil solution pH. Mean soil solution pH of A and B horizons in P. koraiensis was lower than 4.7, however the $Al^{3+}$ concentration was lower than the toxic level to plants.

• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1726-1728
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• 1999

Dielectric and piezoelectric properties of xPb$(Al_{1/2}Ta_{1/2})O_3$-(1-x)Pb$(Zro_{0.52}Ti_{0.48})O_3$ system were investigated. The highest density of $7.80g/cm^3$ for PAT-PZT ceramics of 5mol% PAT was obtained. The relative permittivity of PAT-PZT ceramics of 5mol% PAT was 1.642 at room temperature. The maximum value of electromechanical coupling factor $k_p$ of 55% and $k_t$ of 33% were obtained at the composition of 5mol% PAT. The grain sizes were reduced by increasing the amount of PAT, however mechanical quality factor$(Q_m)$ had a minimum value of 44 at the composition of 5mol% PAT.