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Recovery Rate of Nontuberculous Mycobacteria from Acid-Fast-Bacilli Smear-Positive Sputum Specimens (항산균 도말양성 객담에서 비결핵성 마이코박테리아의 분리 비율)

  • Koh, Won-Jung;Kwon, O Jung;Yu, Chang Min;Jeon, Kyeongman;Suh, Gee Young;Chung, Man Pyo;Kim, Hojoong;Han, Sang Won;Park, Sun Young;Lee, Nam Yong
    • Tuberculosis and Respiratory Diseases
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    • v.54 no.1
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    • pp.22-32
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    • 2003
  • Background : Sputum smears for acid-fast bacilli(AFB) examined microscopically is the most important diagnostic test for pulmonary tuberculosis. However, the AFB observed on the smear may represent either M. tuberculosis or nontuberculous mycobacteria (NTM). This study examined the recovery rate of NTM from the AFB smear-positive sputum specimens in a tertiary hospital in Korea. Materials and Methods : This study analyzed the results for the 1,889 AFB smear-positive and culture-positive sputum specimens collected from 844 patients from July, 1997 to December, 2001. Results : The 1,889 sputum specimens collected from 844 patients tested positive on both microscopy and culture during the 4.5 years. The NTM were recovered from 10.3% (195/1,889) of the smear-positive sputum specimens and 11.0% (93/844) of patients with smear-positive sputum. The NTM were isolated more than two times in 44.1% (41/93) of the patients from whom the NTM was recovered. Trends of the recovery rate of the NTM from the AFB smear-positive sputum specimens were increasing from 6.5%(17/262) in the latter half of 1997 to 17.8%(36/202) in the latter half of 2001 (p<0.001, test for trend). Conclusions : These results suggest that some patients with AFB smear-positive sputum have NTM pulmonary disease rather than pulmonary tuberculosis in Korea.

Deformation History of Precambrian Metamorphic Rocks in the Yeongyang-Uljin Area, Korea (영양-울진 지역 선캠브리아기 변성암류의 변형작용사)

  • Kang Ji-Hoon;Kim Nam Hoon;Park Kye-Hun;Song Yong Sun;Ock Soo-Seok
    • The Journal of the Petrological Society of Korea
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    • v.13 no.4
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    • pp.179-190
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    • 2004
  • Precambrian metamorphic rocks of Yeongyang-Uljin area, which is located in the eastern part of Sobaegsan Massif, Korea, are composed of Pyeonghae, Giseong, Wonnam Formations and Hada leuco granite gneisses. These show a zonal distribution of WNW-ESE trend, and are intruded by Mesozoic igneous rocks and are unconformably overlain by Mesozoic sedimentary rocks. This study clarifies the deformation history of Precambrian metamorphic rocks after the formation of gneissosity or schistosity on the basis of the geometric and kinematic features and the forming sequence of multi-deformed rock structures, and suggests that the geological structures of this area experienced at least four phases of deformation i.e. ductile shear deformation, one deformation before that, at least two deformations after that. (1) The first phase of deformation formed regional foliations and WNW-trending isoclinal folds with subhorizontal axes and steep axial planes dipping to the north. (2) The second phase of deformation occurred by dextral ductile shear deformation of top-to-the east movement, forming stretching lineations of E-W trend, S-C mylonitic structure foliations, and Z-shaped asymmetric folds. (3) The third phase deformation formed I-W trending open- or kink-type recumbent folds with subhorizontal axes and gently dipping axial planes. (4) The fourth phase deformation took place under compression of NNW-SSE direction, forming ENE-WSW trending symmetric open upright folds and asymmetric conjugate kink folds with subhorizontal axes, and conjugate faults thrusting to the both NNW and SSE with drag folds related to it. These four phases of deformation are closely connected with the orientation of regional foliation in the Yeongyang-Uljin area. 1st deformation produced regional foliation striking WNW and steeply dipping to the north, 2nd deformation locally change the strike of regional foliation into N-S direction, and 3rd and 4th deformations locally change dip-angle and dip-direction of regional foliation.

Detection of Pathogenic Yersinia Enterocolitica in Drinking Water and Vegetables by Mutiplex-PCR (Multiplex-PCR에 의한 먹는샘물 및 야채류로부터의 병원성 Yersinia enterocolitica의 신속검출)

  • 이택수;박부길;오덕환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.35-41
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    • 2003
  • The study was conducted to develope a rapid method for the detection of Yersinia enterocolitica in spring water and vegetables via multiplex polymerase chain reaction (PCR) technique using ail, yst, uirF and subgenus-specific Y16S primers. Specificity and sensitivity of multiplex PCR and application of best primers for the detection of Y. enterocolitica from spring water and vegetables were investigeted. Y. enterocolitica ATCC 27729 strains gave 356 bP and 200 bp (Y16S) and 134 bp (yst) bands. but Y. enterocolitica ATCC 9610 and ATCC 23715 strains gave 200 bp and 134 bp bands.In the meanwhile, non-pathogenic Yersinia species, such as Y. frederikseni, Y. inter-media, Y. kristenseni and Y. pseudotuberculosis gave only single 200 bp band, and other bacteria including Escherichia coli O157:H7 ATCC 25392, Shigella dysenteri. Staphylococcu aureus ATCC 25923 and Listeria mo-nocytogenes ATCC 19111 did not show any bands. Among primers, yst and Y16S primer showed the best sensitivity. Seven CFU/mL Y. enterocolitica cells could be detected with yst and Y16S primers and the sensitivity was significantly improved by the further 2nd PCR after 38 cycles of first PCR amplication. Spring water, cabbage and mushroom were inoculated with Y. enterocolitica to determine the sensitivity of multiplex-PCR for the rapid detection of Y. enterocolitica. Multiplex-PCR assay could detect 7 or 70 cells in spring water and vegetables using whole cell lysate with repeating PCR amplication.

Effect of the Particle Size and Unburned Carbon Content on the Separation Efficiency of Fly ash in the Countercurrent Column Flotation (向流컬럼浮選機에서 石炭灰의 크기 및 未燃炭素 含量이 分離特性에 미치는 영향)

  • 이정은;이재근
    • Resources Recycling
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    • v.9 no.6
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    • pp.36-44
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    • 2000
  • Fly ash was composed of the unburned carbon and mineral particles. The former was able to attach on the bubbles, while the latter was not. Therefore, it was possible to separate the unburned carbon and the mineral from fly ash using the froth flotation process. This study was carried out to evaluate the separation efficiency as a function of the ny ash particle properties in the column flotation. Separation efficiency was analyzed for various size fraction of -38 fm,38~125 fm and 1125 W, and for various fly ash samples containing 7, 11, and 20 wt% unburned carbon. For the size fractions of -38 fm containing 7 wt% unburned carbon, separation efficiency was 86ft, whereas separation efficiency was found to be 74% for the size fraction of +125$\mu\textrm{m}$ containing 20 wt% unburned carbon. The results indicated that separation efficiency increased with the decrease in the particle size and the unburned carbon content of the fly ash.

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Effectiveness of controlled atmosphere container on the freshness of exported PMRsupia melon (CA 컨테이너를 이용한 수출 멜론의 선도유지 효과)

  • Haejo Yang;Min-Sun Chang;Puehee Park;Hyang Lan Eum;Jae-Han Cho;Ji Weon Choi;Sooyeon Lim;Yeo Eun Yun;Han Ryul Choi;Me-Hea Park;Yoonpyo Hong;Ji Hyun Lee
    • Food Science and Preservation
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    • v.30 no.5
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    • pp.822-832
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    • 2023
  • This study investigates the effectiveness of CA (controlled atmosphere) containers in maintaining the freshness of exported melons. The melons were harvested on June 5, 2023, in the Yeongam area of Jeollanam-do, Korea. The CA container was loaded with melon samples packed in an export box. The temperature inside the container was set at 4℃, while the gas composition was set at 5% oxygen, 12% carbon dioxide, and 83% nintrogen. Following two weeks of simulated transportation, quality analysis was conducted at 10℃. The melons were inoculated with spore suspensions, and the decay rate was determined to investigate the effect of the gas composition inside the CA container on suppressing the occurrence of Penicillium oxalicum in melons. The results were compared with a Reefer container set at the same temperature. The samples transported in the CA container exhibited lower weight loss. The melon pulp softening, respiration rate, and ethylene production were slower using the CA container. Moreover, the decay rate during the distribution period in the CA container was lower than in the Reefer container. In contrast, the firmness of melons transported in the Reefer container decreased significantly (from 9.03N to 5.18N) immediately after transportation. The soluble solid content (SSC) of melons transported in the Reefer container also decreased rapidly. The results suggested that the CA container is the optimal export container for maintaining the freshness of melons.

Changes in residential patterns by the age composition in Cheongju city, Korea (年齡別 人口構成에서 본 淸州市의 居住패턴 變化)

  • ;Han, Ju-Seong
    • Journal of the Korean Geographical Society
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    • v.30 no.1
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    • pp.57-67
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    • 1995
  • The study on the factorial ecology of the residential patterns is to provide one of the yardsticks for description and comparison of urban structures. Many Korean geographers have adopted this method to analyse the urban structure of Korean cities. According to these studies, one of the main factors in Korean cities in large and middle sizes is family status. The spatial pattern of family status is zonal, similar to the cases of Japanese and Western cities. The age is one of the principal indices of familyf status, hence the author chose the age composition to analyse the residential patten. This Paper is to describe the residential segreagation pattern due to age composition and recent pattern changes in the Cheongju city, and than to explain the reason for these changes. All data are derived from the Population Censuses of Korea for 1970, 1980 and 1990. Eighteen groups of age with five-year interval (0-4, 5-9, 10-14, 15-19, 20-24, 25-29, 30-34, 35-39, 40-44, 45-49, 50-54, 55-59, 60-64, 65-69, 70-74, 75-79, 80-84, 85- and- over) are adopted here. Unit area for this analysis is administrative district(Dong) within Cheonaju city. District are classified into smaller groups based on the similarity of age composition, using the method of cluster analysis. The main findings are summarized as follows: 1. Population have increased remarkably in the eastern reaion neighboring CBD of Cheongju city in 1970's. And in western region from CBD new residential area have developed in 1980's. 2. Spatial pattarns showed a concentric circle type in central district and its neighbor regions and a sector type in periphery regions in 1970; a cirele type in central district and a sector typesin neighbor regions and periphery regions in 1980 and 1990. Thess residential pattern play an important role in the population composition ratio of younger aged group (l5-34) and older aged group (65-and-over). 3. Spatial change of types by age composition showed the higher ratio of groups of 0-9 and 35-49, and lower ratio of group 20-24 in 1970's. Dominent groups are ratio of 0-14, 40-49, 55-64, 7O-79, and 85- and- over in 1980's. These changes mainly appeared in central district and periphery regions. 4. The reasons for the change of age composition was the development of msnufacturing industries with the increase of population and new construction of residential areas both in the neighbor regions of cnetral district and periphery regions. These phenomena were caused by immigration of younger aged groups and increasing of residents of aged groups in these regions.

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Usefulness of the quantitative analysis by using the functional parameter index from dynamic salivary glands scintigraphy (동적 타액선 신티그라피에서 기능적 매개변수 지표를 활용한 정량 분석의 유용성)

  • Shin, Chae Ho;Kim, Bong Su;Cho, Yong Gyi;Jo, Jin Woo;JIn, Sung Eun;Pyo, Sung Jai
    • The Korean Journal of Nuclear Medicine Technology
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    • v.19 no.2
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    • pp.81-86
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    • 2015
  • Purpose This study was to evaluate the usefulness through the comparison of patients group and healthy control group by acquiring a variety of functional parameters index from time-activity curves of salivary gland scintigraphy using $^{99m}Tc$. Materials and Methods From December 2014 to February 2015 with the targets of 30 patients in the patient group who presented themselves with xerostomia and underwent the salivary gland scan and 10 people in healthy control group, approximately 30 minutes of dynamic Scan was carried out after the intravenous bolus injection of 370 MBq of $^{99m}TcO_4{^-}$ and per os stimulation with Vitamin C powder 20 minutes after the injection. The method of quantitative analysis was as follows, the time-activity curve was drawn after the parotid gland and submandible gland were prescribed as a region of interest, a variety of functional parameters index was obtained in each position of the curve, and the patients group and the control group were compared. Results As for the methods applied in comparison and measurement, uptake ratio (UR), time at maximum counts (Tmax), time at minimum counts (Tmin), maximum accumulation (MA), accumulation velocity, maximum secretion (MS), maximum stimulation secretion (MSS), and secretion velocity (SV) were used. In the comparison of functional parameters index of patient group and normal group, the healthy normal group showed significant difference compared to the patient group in all indices except for the minimum radioactivity time (Tmin), and also in terms of variation over time the normal group showed significant difference compared to the patient group (p<0.05). Conclusion Consequently, it was considered that the quantitative analysis that used a variety of functional parameters index would be useful for evaluating the function of the salivary glands of the patients with xerostomia as an objective and standardized information.

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Upper Body Surface Change Analysis using 3-D Body Scanner (3차원 인체 측정기를 이용한 체표변화 분석)

  • Lee Jeongran;Ashdoon Susan P.
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.12 s.148
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    • pp.1595-1607
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    • 2005
  • Three-dimensional(3-D) body scanners used to capture anthropometric measurements are now becoming a common research tool far apparel. This study had two goals, to test the accuracy and reliability of 3-D measurements of dynamic postures, and !o analyze the change in upper body surface measurements between the standard anthropometric position and various dynamic positions. A comparison of body surface measurements using two different measuring methods, 3-D scan measurements using virtual tools on the computer screen and traditional manual measurements for a standard anthropometric posture and for a posture with shoulder flexion were $-2\~20mm$. Girth items showed some disagreement of values between the two methods. None of the measurements were significantly different except f3r the neckbase girth for any of the measuring methods or postures. Scan measurements of the upper body items showed significant linear surface change in the dynamic postures. Shoulder length, interscye front and back, and biacromion length were the items most affected in the dynamic postures. Changes of linear body surface were very similar for the two measuring methods within the same posture. The repeatability of data taken from the 3-D scans using virtual tools showed satisfactory results. Three times repeated scan measurements f3r the scapula protraction and scapula elevation posture were proven to be statistically the same for all measurement items. Measurements from automatic measuring software that measured the 3-D scan with no manual intervention were compared with the measurements using virtual tools. Many measurements from the automatic program were larger and showed quite different values.

Clinical Analysis of the Acute Respiratory Distress Syndrome after Thoracotomy (개흉술 후 발생하는 급성 호흡부전 증후군에 대한 임상적 고찰)

  • 이용직;박승일;제형곤;박창률;김동관;주석중;김용희;손광현
    • Journal of Chest Surgery
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    • v.35 no.9
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    • pp.653-658
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    • 2002
  • The cause and clinical course of the postoperative ARDS is, as of yet, not very well understood. The current study is a review of our experience on patients with ARDS after thoracotomy. Material and Method: Between Jan. 1996 to Aug. 2001, a retrospective analysis was conducted on 32 post-thoracotomy ARDS patients among 4018 patients receiving thoracotomy inclusive of thoracoscopic surgery. Result: The incidence of ARDS after pneumonectomy cases was 5.3%(13/245), 1.3% after lobectomy(9/ 710), and 4.4% after esophageal surgery(10/226). Of the 32 ARDS patients, 31 had malignant disease. The remaining 1 patient had aspergillosis. In the majority, the cause of ARDS was unknown. The average onset was on the 7.4th postoperative day. In 10 cases, the initial lesion was in the right lower lung field(31.2%), in the left lower lung field in 9(28.1%), and in both lower lung fields in 12(37.5%) cases. In all, the initial lesion was in the lower lung fields in 96.9% of the cases(31/32). There was a significant relationship between the development of ARDS and intraoperative I/O balance. The overall mortality rate was 65.6%(21/32). In the earlier period of the study(1996-Jun, 1998) the mortality rate was 100%, but in the latter period(July, 1998-Aug, 2001) it was significantly reduced to 47.6%: Conclusion: The current data showed a higher incidence of postoperative ARDS in patients with malignant disease and in those receiving extensive lymph node dissection with either lobectomy or pneumonectomy, and also in patients receiving esophageal surgery. In addition, introperative fluid overload was also associated with an increased incidence of ARDS. Treatment outcome could be improved with prone positioning and NO gas inhalation.

Inhibitory Effects of Lentinus edodes and Rice with Lentinus edodes mycelium on Diabetes and Obesity (In vitro 및 In vivo에서 표고버섯, 표고버섯균사체배양쌀 추출물의 항당뇨와 항비만 작용)

  • Kim, Haeseop;You, Jeheon;Jo, Yeongcheol;Lee, Youngjae;Park, Inbae;Park, Jeongwook;Jung, Myung-A;Kim, Young-Suk;Kim, Sunoh
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.2
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    • pp.175-181
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    • 2013
  • This study investigated the ability of extracts from Lentinus edodes (LE) and rice with Lentinus edodes mycelium (LEM) to inhibit diabetes and obesity. Lipid accumulation significantly decreased by 78% and 74% upon treatment with 300 ${\mu}g/mL$ of LE and LEM, respectively (p<0.01). Cholesteryl ester transfer protein (CETP) inhibition activity increased by 94% and 99% upon treatment with 300 ${\mu}g/mL$ of LE and LEM, respectively. In order to investigate the effect of LE and LEM on diabetes, the inhibition of protein tyrosine phosphate 1B (PTP1B) activity from the LE and LEM extracts at various concentrations (1, 3, 10, 30, 100, 300 ${\mu}g/mL$) was assessed. PTP1B activity by treatment with 10, 30, and 100 ${\mu}g/mL$ of LE, was inhibited at a rate of 7, 9, and 7% respectively. Also, PTP1B activity from treatment with increasing concentration of LEM led to a significant concentration-dependent inhibition of PTP1B activity (p<0.01). LE and LEM were orally administered for 28 days after a high fat diet (HFD). LE and LEM significantly reduced triglyceride, cholesterol, HDL-cholesterol and LDL-cholesterol levels. GOT and GPT were not significantly effected. These results indicate that extracts of LE and rice with LEM have potent activities useful in the treatment of obesity and diabetes mellitus.