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Microbiological Evaluations of Refregerated Flatfish Treated with Organic Acids (유기산으로 처리한 냉장 광어의 미생물 평가)

  • 김창렬;김정숙;고대희;최운정;이극로;강어진;김광현
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.329-333
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    • 1998
  • The effects of 0.5%(v/v) acetic acid (AA), 0.5%(v/v) lactic acid(LA), or 0.5%(v/w) citric acid(CA) on gram-negative bacterial counts (GNC) and aerobic plate counts (APC) in flatfish strips stored at 4$^{\circ}C$ or 1$0^{\circ}C$ were assessed. Treatments of 0.5% AA were completely (P<0.05) inhibited GNC compared to the initial controls for 12 days at 4$^{\circ}C$. Treatments of either LA or CA rapidly increased GNC compared to those of AA after 9 days at 4$^{\circ}C$. Treatments of AA were microbiological acceptable for 6 days at 1$0^{\circ}C$, but those of LA and CA did not lasted. AA treatments were completely (P<0.05) inhibited aerobic spoilage bacteria (APC) compared to the initial controls for 6 days at 4$^{\circ}C$. Treatments containing either 0.5% LA or 0.5% CA were ineffective after 9 days at 4$^{\circ}C$ and 3 days at 1$0^{\circ}C$, respectively. Based on these results, AA treatment was the most effective for suppressing the growth of aerobic spoilage bacteria during storage at both 4 and 1$0^{\circ}C$. Flatfish strips treated with 0.5% AA extended microbiological shelf-life for 12 days at 4$^{\circ}C$.

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Distribution and Characteristics of Acidotolerant Heterotrophic and Naphthalene­Degrading Bacteria in Acidic Soils (산성토양에서 내산성 종속영양세균과 나프탈렌분해세균의 분포 및 특성)

  • Moon Yong-Suk;Chu Kwang-Il;Kim Jongseol
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.313-319
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    • 2004
  • The distribution and characteristics of acidotolerant heterotrophic and naphthalene-degrading bacteria were investigated in two forest areas, one near Ulsan petrochemical industrial complex (Sunam) and the other in countryside (Daeam). Average values of soil pH at Sunam and Daeam were 3.8 and 4.6, respectively. When het­erotrophic and naphthalene-degrading bacteria were enumerated by most probable number (MPN) procedures at Sunam, the median values of heterotrophs growing at pH 7.0 and pH 4.0 were $5.3{\times}10^7\;and\;3.3{times}10^7$ MPN/g, whereas those of naphthalene-degraders were $5.6{\times}10^4\;and\;4.0{times}10^5$ MPN/g, respectively. While the medians of heterotrophs at Daeam were larger than those at Sunam, the concentrations of naphthalene-degraders were higher at Sunam compared to those at Daeam. From the MPN tubes and enrichment cultures, we obtained 17 isolates of naphthalene-degraders which were identified as Sphingomonas paucimobilis, Brevundimonas vesic­ularis, Burkholderia cepacia, Ralstonia pickettii, Pseudomanas fluorescens, and Chryseomonas luteola. Among them, 6 isolates showed higher naphthalene-degrading activity on minimal media of pH 4 compared to pH 7, whereas the extent of growth was not greater at pH 4 than at pH 7 when they were inoculated on nutrient-rich media. It is plausible that the pH may affect naphthalene-degrading activity of the isolates by changing fatty acid composition of bacterial membrane.

Temperature and Sweet Corn Production at Different Planting Dates under Polyethylene Tunnel and Mulch (파종기와 Polyethylene 필름 피복방법이 단옥수수 생산에 미치는 영향)

  • 이석순;김태주
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.1
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    • pp.84-90
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    • 1986
  • An experiment was conducted to know the effects of planting dates (March 5, 15, and 25 and April 4) and transparent polyethylene (P.E.) film treatments(tunnel, tunnel slit, and mulch) on air and soil temperatures and growth and yield of a sweet corn variety, Great Bell. Maximum air and soil temperatures and minimum air and soil temperatures were greater at tunnel>tunnel slit>mulch in that order. Differences in maximum air and soil temperatures among the P.E. film treatments were much greater than those in minimum air and soil temperatures. However, when film was opened due to high air temperature over 40$^{\circ}C$ in the tunnel, air temperature was similar but soil temperature was lower com-pared to mulch. High temperature stress could be avoided in tunnel slit without opening film by increase in the number of slits. Cold damage of corn seedlings was avoided by tunnel and reduced by tunnel slit, and frost-damaged seedlings under the mulch were recovered in few days. The number of days from planting to silking was reduced as planting date delayed. At early plantings, tunnel enhanced early growth and silking, but it delayed at late plantings because tunnel was opened during the most of day time due to high temperature. Black streaked dwarf virus(BSDV) disease was more severe at early plantings and it was reduced in tunnel slit at late plantings because plants were grown under the film at the time of infection. The number of marketable ears was similar among all treatments except mulch at March 5 planting where BSDV was severely infected. Gross income was high in tunnel and tunnel slit at March 25 planting which had more larger marketbale ears and tunnel and tunnel slit at March 5 planting which had higher market price.

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Ca2+ Regulators affect the Gravitropism and Ethylene Production Induced by Malformin A1 in Maize Root (옥수수 뿌리에서 칼슘 이온 조절제가 malformin A1에 의해 유도된 굴중성과 에틸렌 생합성에 미치는 영향)

  • Hong, Sung-Hyun;Oh, Seung-Eun;Kim, Kun-Woo;Jeong, Hyung-Jin;Kim, Soon-Young
    • Journal of Life Science
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    • v.17 no.2 s.82
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    • pp.174-178
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    • 2007
  • Treatment of malformin A1 is known to increase ethylene production 130% at 4 hr and 56% at 8 hr after treatment in maize root compared to untreated plants. The ethylene production by malformin A1 was maximum level at 4 hr and slowly decreased up to 8 hr. Calcium ion regulators such as A23187 (calcium ionophore) and verapamil (calcium channel blocker) stimulated ethylene production. Treatment of both calcium ion regulators increased about 30% of ethylene production at 4 hr, and 20% at 8 hr. Both calcium ion regulators did not stimulate malformin A1-induced ethylene production at 4 hr as malformin A1 itself did. However, the treatment of calcium ion regulators with malformin A1 maintains the ethylene production for 8 hr. These results suggested that the proper concentration of calcium might need to confer the effect of malformin A1 on the ethylene production. Malformin A1 suppressed the gravitropic curvature of maize root about 58% at 4 hr and 42% at 8 hr compared to control plant. Verapamil inhibited the gravitropic curvature about 54% at 4 hr and 23% at 8 hr compared to control, respectively. But A23187 could not. In addition, verapamil showed more inhibition in malformin A1-induced gravitropic curvature than A23187 in malformin A1 induced. These data suggested that calcium ion regulators affect the malformin A1-induced ethylene production and gravitropic curvature, and give the evidence that calcium ion play an important role in gravitropic curvature in maize root.

Detection of short-term flux variability and intraday variability in polarized emission at millimeter-wavelength from S5 0716+714

  • Lee, Jeewon;Sohn, Bong Won;Byun, Do-Young;Lee, Jeong Ae;Lee, Sang Sung;Kang, Sincheol;Kim, Sungsoo S.
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.33.1-33.1
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    • 2016
  • We report detection of short-term flux variability in multi-epoch observations and intraday variability in polarized emission at millimeter-wavelength from S5 0716+714 using Korean VLBI Network (KVN) radio telescopes. Over the whole observation epochs, the source shows significant inter-month variations at K- and Q-band with modulation indices of 19% at K-band and 36% at Q-band. In each epoch, the source shows monotonic flux increase in Epoch 1 and 3, and monotonic flux decrease in Epoch 2 and 4. We found an inverted spectrum with mean spectral indices of -0.57 in Epoch 1 and -0.15 in Epoch. On the contrary, we found relatively steep indices of 0.24 and 0.17 in Epoch 2 and Epoch 4, respectively. In the study of intraday variability of polarization, we found significant variations in the degree of linear polarization at 86 GHz, and in polarization angle at 43 and 86 GHz during ~10 h. The spectrum of the source is quite flat with spectral indices of -0.07 to 0.07 at 22-43 GHz and -0.23 to 0.04 at 43-86 GHz. The measured degree of the linear polarization ranges from 2.3% to 3.3 % at 22 GHz, from 0.9% to 2.2 % at 43 GHz and from 0.4 % to 4.0 % at 86 GHz, yielding prominent variations at 86 GHz over 4-5 h. The linear polarization angle is in the range of 4 to $12^{\circ}$ at 22 GHz, -39 to $81^{\circ}$ at 43 GHz, and 66 to 119 at 86 GHz with a maximum rotation of $110^{\circ}$ at 43 GHz over ~4 h. We estimated the Faraday rotation measures (RM) ranging from -9200 to 6300 rad m-2 between 22 and 43 GHz, and from -71000 to 7300 rad m-2 between 43 and 86 GHz, respectively. The frequency dependency of RM was investigated, yielding a mean power-law index, a, of 2.0. This implies that the polarized emission from S5 0716+714 at 22-86 GHz moves through a Faraday screen in or near the jet of the source.

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A Study on Ion Distribution and Behavior of Acidrain in Mokpo and Yeochon Area (목포, 여천지역 강수의 무기이온 성분농도와 거동에 관한 연구)

  • 오길영;양수인;이완진
    • Journal of Korean Society for Atmospheric Environment
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    • v.15 no.4
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    • pp.385-392
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    • 1999
  • This study was carried out to investigate the constituents of inorganic ions of precipitation in Mokpo and Yeochon area from Jan. 1996 to Dec. 1997. The volume-weighted mean pH of precipitation was 5.7(4.8~7.8) at Mokpo, and 5.6(5.1~7.4) at Yeochon area, respectively. The non-seasalt(nss) anion concentrations were found in order of $nss-SO^{2-}_4>NO^-_3>nss-Cl^-$ at two areas. The portion of $nss-SO^{2-}_4;and;NO^-_3$ was 71~84% out of anions. The the non-seasalt cation concentrations were found in order of $NH^+_4>nss-Ca^{2-}>nss-Mg^{2+}>nss-K^-$ at two areas. The portion of $NH^+_4;and; nss-Ca^{2+}$ was 85~92% out cations. Compared regionally the year concentraton of $nss-SO^{2-}_4$, the result of Yeochon was 3 times higher than that of Mokpo in 1996, while the results of two regions were almost similar in 1997. The reason was that Yeochon was restricted area of the use of 0.5% sulfur B-C oil, controlled air pollution emission area, controlled semitotal amounts of air pollution emission area and partial operating of factories in 1997. There were no ions having high correlation with $H^+,;but;nss-SO^{2-}_4,;NO^-_3,;NH^+_4;and;nss-Ca^{2+}$ showed high correlation coefficient each other. It seems that these ions have little correlation with $H^+$ because they are washed out on binding state. Factor analysis showed that the first factor was complicated factor containing anthropogenic and soil resource, the second factor was sea-salt resource and the third factor was independent behavior of hydrogen at Mokpo. While, the first factor was complicated factor containing anthropogenic and sea-salt resource, the second factor was $Ca(NO_3)_2$ salt and the third factor was $NH^+_4;and;SO^{2-}_4$ synergied by resource and combination at Yeochon.

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Clinical Study in 17cases of patients for sinusitis(treated with aroma-therapy) (향기요법(香氣療法)을 병행(幷行)한 소아비연(小兒鼻淵) 환자(患者) 17예(例)에 대한 임상적(臨床的) 연구(硏究))

  • Han Jae-Kyung;Kim Yun-Hee;Kim Yeon-Jin
    • The Journal of Pediatrics of Korean Medicine
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    • v.14 no.2
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    • pp.105-120
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    • 2000
  • Clinical studies were done treated with aroma-therapy in 17cases of patients for suffering sinusitis, who were treated at Dept. of Pediatrics in the Hospital of Daejon from March 2000 to November 2000. The results were as follows. 1. In distribution of sex & age, 70.6% was men and 29.4% women. 47.1% was between 5 and 7 years old, 35.3% within 5 years old. 2. In distribution of the period of the clinical history, 35.3% was over 6 momths, 29.4% of them between 6 and 12 months. 3. In the living environment the apartment was 58.5% and the residental zone is 29.4%. 4. The incurrence of season is winter, 47.0% next is change of season, 29.4%. 5. At night the symptom is the most common, 52.9%. In the morning is the next, 35.3%. 6. In the past history. tinnitus was the best at 38.4%. The otitis media. atopic dermatitis, convulsion disease is next at 15.4%. 7. In the family history. the allergic rhinitis or sinusitis is the most rich at 38.4% and tinnitus is next at 23.0%. 8. In distribution of the chief complain companied with sinusitis, 23.3% was nasal obstruction, each 18.4% of them nasal discharge, cough. 9. In distribution of the duration of therapy, 29.4% was over 6 months, 23.5% between 5-6 months. In distribution of the therapeutic method, 47.1% was treated with western and oriental medicine and 42.1% only oriental medical care. 10. In distribution of the period of improvement of symptom(by X-ray reading), 35.2% was between 2-3 momths, 29.4% between 1-2 months. 11. In emotional change, after aroma-therapy, 82.4% of them felt good. 12. After treatment, 70.6% was excellent, 17.6% was good.

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Planar Structural Requirement at 4-position of 1-Arylsulfonyl-4-phenyl-4,5-dihydro-2-imidazolones for their Cytotoxicity

  • Jung, Sang-Hun;Kwak, Suhk-Jun
    • Archives of Pharmacal Research
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    • v.20 no.3
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    • pp.283-287
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    • 1997
  • In order to identify the structural requirement at 4-position of 1-arylsulfonyl-4-phenyl-4,5-dihydro-2-limidazolones 3 for their cytotoxicity, the corresponding 1-arylsulfonyl-4-cyclohexyl-4,5-dihydro-2-imidazolones 4 were synthesized and their in vitro cytotoxicity against human solid tumor cell lines were measured. Unlike compounds 3a-c, cyclohexyl analogues 4a-c do not show the cytotoxicity. This dramatic loss of activity of these analogues on the volume change with the bulkier cyclohexyl group indicates that the planar structure at 4-position of 1-arylsulfonyl-4-pheny-4,5-dihydro-2-limidazolones 3 is required for their activity as an important pharmacophoric moiety.

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Studies on the Viability of Frozen Removed Seminal Plasma by Saline(RSP-S) and Tris-buffer(RSP-T) Semen of Small Spcies Dogs

  • Kim, S. K.
    • Proceedings of the KSAR Conference
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    • 2001.10a
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    • pp.57-57
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    • 2001
  • This study was carried out to investigate the general characteristics such as volume, sperm concentration, sperm motility, sperm abnormality on whole semen, RSP-S and RSP-T semen and fractional semen of small size dogs, and the effect of temperature and preservation time and cryoproservation on motility of whole and RSP-S and RSP- T semen. Multiple ejaculates were collected from small dogs by the digital manipulation of penis. 1. The volume per ejaculate semen, sperm of concentration and motility and abnormal sperm rate of 1st fractional semen were 0.65±0.09㎖, 4.52±0.35×10/sup 6/ cells/㎖, 15.64±3.85% and 5.50±0.62%. Also, 2nd fractional semen were 1.25±0.20㎖, 3.35±0.48×10/sup 6/cells/㎖, 96.25±4.65% and 4.24±0.46%. And 3rd fractional semen were 1.45±0.21㎖, 3.85±0.52×10/sup 6/cell/㎖, 92.82±4.24% and 4.66±0.58%, respectively. 2. The sperm of concentration and motility and abnormal sperm rates of whole, RSP-S and RSP-T semen were 5.45±0.82×10/sup 6/ cells/㎖, 95.55±4.65%, 4.58±0.45% and 4.82±0.36×10/sup 6/cells/㎖, 90.10±3.42%, 6.48±0.68% and 4.55±0.45× 10/sup 6/cells/㎖, 93.25±3.85%, 4.82±0.58%, respectively. 3. The motility of whole, RSP-S and RSP-T semen were higher at 4℃ than at 38℃. When preservation temperature was at 4℃, survival rates of RSP-S and RSP-T sperm were 97.54%-6.25% at 1-72 hrs, 97.40%-5.62% at 1-100 hrs, respectively. 4. The survival rates of slow and rapid frozen 2nd fraction, RSP-S and RSP-T semen were 67.3±4.45%, 88.8±4.46% and 46.4±3.84%, 74.4±4.20%, respectively. Survival rates was significantly higher in frozen RSP-S and RSP-T semen than that in control group(8.5±2.12%).

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Flowfield Characteristics of a Rotating Circular Cylinder Having Square Dimpled Surface (정방형 딤플을 가진 회전원주의 유동장 특성)

  • Ro, Ki-Deok;Kang, Myeong-Hoon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.28 no.4
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    • pp.486-492
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    • 2004
  • Flow patterns around a rotating circular cylinder having square dimpled surface were visualized by the hydrogen bubble technique at velocity ratios from a=0 to 4.8 and Reynolds number of Re=1.0${\times}$10$^4$. The wake region of the cylinder was reduced as the velocity ratios increase and was smaller than that of the smooth cylinder without dimples at the same velocity ratio. The hydrodynamic characteristics on the cylinder was investigated by measuring of lift and drag at velocity ratios from a=0 to 4.1 and Reynolds number from Re=1.2${\times}$10$^4$ to Re=2.0${\times}$10$^4$. As the velocity ratios increase, the average lift and drag coefficients were increased and at the same velocity ratio, the average lift was larger but the average drag was smaller than that of the smooth cylinder.