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유기산으로 처리한 냉장 광어의 미생물 평가 (Microbiological Evaluations of Refregerated Flatfish Treated with Organic Acids)

  • 김창렬;김정숙;고대희;최운정;이극로;강어진;김광현
    • 한국식품영양학회지
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    • 제11권3호
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    • pp.329-333
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    • 1998
  • 본 연구는 4~1$0^{\circ}C$ 냉장조건에서 0.5% 초산(AA), 0.5% 유산 (LA) 및 0.5% 구연산(CA) 침지법으로 5분 동안 처리한 신선한 광어의 미생물학적 저장 안정성에 미치는 영향을 조사하였다. 0.5% 초산 처리구는 4$^{\circ}C$, 12일 저장 동안 그람음성세균(GNC)이 증식을 완전히 억제하는데 효과적이었다. 0.5%의 유산 및 구연산 처리구는 각각 4$^{\circ}C$, 저장 9일부터 그람음성세균(GNC)의 급속한 증식을 보였다. 1$0^{\circ}C$에서 6일 저장 동안 0.5% 초산 처리구는 그람음성균의 증식억제에 효과적이었으며, 유산 및 구연상 처리구는 저장 3일 이후 효과가 없었다. 0.5%(v/v) 초산 처리구는 4$^{\circ}C$에서 저장 6일 동안 그리고 1$0^{\circ}C$에서 저장 3일 동안 호기성 부패 세균(APC)의 증식을 완전히 억제하였다. 0.5%(v/v) 초산 처리구는 4$^{\circ}C$에서 저장 12일 동안, 그리고 1$0^{\circ}C$에서 저장 6일 동안 미생물학적 저장 안정성을 유지하였다. 본 연구의 결과 4~1$0^{\circ}C$에서 광어포의 저장 도안 호기성 육부패의 주요 세균인 그람음성세균(GNC) 억제력이 초산 처리구의 광어포는 4$^{\circ}C$에서 12일 동안 미생물학적 저장 안정성을 유지하였다.

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산성토양에서 내산성 종속영양세균과 나프탈렌분해세균의 분포 및 특성 (Distribution and Characteristics of Acidotolerant Heterotrophic and Naphthalene­Degrading Bacteria in Acidic Soils)

  • 문용석;주광일;김종설
    • 미생물학회지
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    • 제40권4호
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    • pp.313-319
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    • 2004
  • 울산석유화학공단과 인접한 지역(선암)및 농촌지역(대암)의 산림토양에서 내산성 종속영양세균 및 나프탈렌분해세균의 분포와 특성을 조사하였다. 토양 pH의 평균은 선암과 대암에서 각각 3.8과 4.6으로 측정되었다. 종속영양세균과 나프탈렌분해세균을 최확수치(MPN)법으로 계수하였으며, 선암의 경우 pH 7.0과 pH 4.0에서 생장하는 종속영양세균 수의 중앙간은 각각 $5.3{\times}10^7\;3.3{\times}10^7$ MPN/dried Soil g이었고, 나프탈렌분해세균 수의 중앙간은 pH 7.0과 4.0에서 각각 $5.6{\times}10^4$$4.0{\times}10^5$ MPN/dried soil g이었다. 대암에서 측정한 종속영양세균수의 중앙간은 두 pH 모두 선암에서보다 많았으나 나프탈렌분해세균의 농도는 선암이 대암보다 높았다. MPN시험관과 농화배양으로부터 17개의 나프탈렌분해세균을 분리하였으며, 이들은 Sphingomonas paucimobilis, Brevundimonas vesicularis, Burkholderia cepacia, Ralstonia pickettii, Pseudomonas fluorescens, Chryseomonas luteola 등으로 동정되었다. 분리한 17개 균주 중 6개는, 최소배지에서의 나프탈렌분해 활성이 PH 7.0에 비해 PH 4.0에서 더 높았으나 영양배지에 접종하였을 때의 생장정도는 pH 4.0이 pH 7.0보다 더 크지 않았다. 배지의 pH가 분리한 세균의 세포막 지방산 조성을 변화시켜 나프탈렌분해 활성에 영향을 주는 것으로 생각된다.

파종기와 Polyethylene 필름 피복방법이 단옥수수 생산에 미치는 영향 (Temperature and Sweet Corn Production at Different Planting Dates under Polyethylene Tunnel and Mulch)

  • 이석순;김태주
    • 한국작물학회지
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    • 제31권1호
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    • pp.84-90
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    • 1986
  • 단옥수수 품종 Great Bell을 3월 5일, 15일, 25일과 4월 4일에 파종하고, 파종기마다 투명 P.E. 필름의 Tunnel, Tunnel slit 및 Mulch가 기온, 지온 및 단옥수수의 생육과 수량에 미치는 영향은 다음과 같다. 1. 최고최저기온과 최고최저지온 모두 Tunnel>Tunnel slit> Mulch의 순으로 높았으며 P.E. 필름 처리간 온도차이는 최고기온 및 지온은 컸으나 최저기온 및 지온은 적었다. 2. 4월 하경에 Tunnel내 기온이 4$0^{\circ}C$가 넘어 필름을 열면 Tunnel구의 기온은 Mulch에서와 같으나 지온은 낮았다. Tunnel slit는 필름을 열지 않고 slit수를 증가시켜 고온장해를 피할 수 있었다. 3. 옥수수 유묘기의 저온피해는 Mulch에서는 지상부가 고사하였으나 재생되었고, Tunnel slit에서는 잎의 일부가 피해를 입었으나 Tunnel에서는 피해가없었다. 4. 3월 5일 및 15일 파종에서 Tunnel은 Tunnel slit나 Mulch보다 초기생육과 출사를 촉진시켰으나 고온으로 낮에 필름을 열었던 3월 25일 및 4월 4일 파종에서는 오히 려 지연시켰다. 5. 파종이 늦을수록 파종에서 출사까지 일수가 단축되어 3월 25일 파종에 비하여 3월 5일 및 15일 파종은 출사가 2~3일 빨랐으며 4월 4일 파종은 6~9일 늦었다. 6. 적조위축병은 한기파종에서 이병율이 높았으며, 3월 25 일 및 4월 4 일 파종의 Tunnel slit에서는 생육초기에 필름을 열지 않아 적조위축병 이병율이 Tunnel 이나 Mulch보다 낮았다. 7. 상품성 있는 웅수수는 적조위축병이 심했던 3월 5일 파종의 Mulch를 제외하면 모든 처리간에 차이가 없었다. 조수입은 상품가치가 큰 웅수가 많았던 3월 25일 파종의 Tunnel 및 Tunnel slit와 가격이 높고 웅수가 많았던 3월 5 일 파종의 Tunnel과 Tunnel slit에서 높았다.

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옥수수 뿌리에서 칼슘 이온 조절제가 malformin A1에 의해 유도된 굴중성과 에틸렌 생합성에 미치는 영향 (Ca2+ Regulators affect the Gravitropism and Ethylene Production Induced by Malformin A1 in Maize Root)

  • 홍성현;오승은;김건우;정형진;김순영
    • 생명과학회지
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    • 제17권2호통권82호
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    • pp.174-178
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    • 2007
  • malformin A1은 옥수수 뿌리에서 대조구와 비교하여 에틸렌 생성을 4시간에는 130%, 8시간에는 56%를 각각 촉진하였다. 에틸렌 생성량이 4시간을 최대로 하여 8시간까지 서서히 증가율이 감소하였다. 칼슘 이온 조절제인 A23187 (calcium ionophore)과 verapamil (calcium channel blocker)을 각각 단독으로 처리한 경우 모두 대조구와 비교하여 4시간에는 30%, 8시간에는 20% 정도로 에틸렌 생성을 촉진하였다. 칼슘 이온 조절제가 malformin A1에 의해 유도된 에틸렌 생성에 미치는 영향을 조사한 결과 4시간에는 malformin A1만 처리하였을 경우에 관찰되는 수준만큼 촉진되지 않았지만 칼슘 이온 조절제의 촉진 효과는 8시간까지 유지되었다. 이러한 결과는 malformin A1이 에틸렌 생성을 촉진하기 위하여 적절한 칼슘 농도가 필요할 가능성을 제시하고 있다. Malformin A1은 굴중성 굴곡 반응을 대조구와 비교하여 4시간과 8시간에 각각 58%와 42% 정도 억제하였다. A23187과 달리, verapamil은 굴중성 굴곡반응을 4시간과 8시간에 대조구와 비교하여 각각 54%와 23%를 억제하였다. 더 나아가 verapamil은 malformin A1에 의해 유도된 굴중성 굴곡 반응을 A23187보다 더 억제하였다. 이러한 결과는 칼슘이온 조절제가 malformin A1에 의해 유도된 옥수수 뿌리의 굴중성 반응에 영향을 주며, 칼슘 이온이 굴중성 반응에 중요한 역할을 한다는 증거를 제시한다.

Detection of short-term flux variability and intraday variability in polarized emission at millimeter-wavelength from S5 0716+714

  • Lee, Jeewon;Sohn, Bong Won;Byun, Do-Young;Lee, Jeong Ae;Lee, Sang Sung;Kang, Sincheol;Kim, Sungsoo S.
    • 천문학회보
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    • 제41권2호
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    • pp.33.1-33.1
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    • 2016
  • We report detection of short-term flux variability in multi-epoch observations and intraday variability in polarized emission at millimeter-wavelength from S5 0716+714 using Korean VLBI Network (KVN) radio telescopes. Over the whole observation epochs, the source shows significant inter-month variations at K- and Q-band with modulation indices of 19% at K-band and 36% at Q-band. In each epoch, the source shows monotonic flux increase in Epoch 1 and 3, and monotonic flux decrease in Epoch 2 and 4. We found an inverted spectrum with mean spectral indices of -0.57 in Epoch 1 and -0.15 in Epoch. On the contrary, we found relatively steep indices of 0.24 and 0.17 in Epoch 2 and Epoch 4, respectively. In the study of intraday variability of polarization, we found significant variations in the degree of linear polarization at 86 GHz, and in polarization angle at 43 and 86 GHz during ~10 h. The spectrum of the source is quite flat with spectral indices of -0.07 to 0.07 at 22-43 GHz and -0.23 to 0.04 at 43-86 GHz. The measured degree of the linear polarization ranges from 2.3% to 3.3 % at 22 GHz, from 0.9% to 2.2 % at 43 GHz and from 0.4 % to 4.0 % at 86 GHz, yielding prominent variations at 86 GHz over 4-5 h. The linear polarization angle is in the range of 4 to $12^{\circ}$ at 22 GHz, -39 to $81^{\circ}$ at 43 GHz, and 66 to 119 at 86 GHz with a maximum rotation of $110^{\circ}$ at 43 GHz over ~4 h. We estimated the Faraday rotation measures (RM) ranging from -9200 to 6300 rad m-2 between 22 and 43 GHz, and from -71000 to 7300 rad m-2 between 43 and 86 GHz, respectively. The frequency dependency of RM was investigated, yielding a mean power-law index, a, of 2.0. This implies that the polarized emission from S5 0716+714 at 22-86 GHz moves through a Faraday screen in or near the jet of the source.

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목포, 여천지역 강수의 무기이온 성분농도와 거동에 관한 연구 (A Study on Ion Distribution and Behavior of Acidrain in Mokpo and Yeochon Area)

  • 오길영;양수인;이완진
    • 한국대기환경학회지
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    • 제15권4호
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    • pp.385-392
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    • 1999
  • This study was carried out to investigate the constituents of inorganic ions of precipitation in Mokpo and Yeochon area from Jan. 1996 to Dec. 1997. The volume-weighted mean pH of precipitation was 5.7(4.8~7.8) at Mokpo, and 5.6(5.1~7.4) at Yeochon area, respectively. The non-seasalt(nss) anion concentrations were found in order of $nss-SO^{2-}_4>NO^-_3>nss-Cl^-$ at two areas. The portion of $nss-SO^{2-}_4;and;NO^-_3$ was 71~84% out of anions. The the non-seasalt cation concentrations were found in order of $NH^+_4>nss-Ca^{2-}>nss-Mg^{2+}>nss-K^-$ at two areas. The portion of $NH^+_4;and; nss-Ca^{2+}$ was 85~92% out cations. Compared regionally the year concentraton of $nss-SO^{2-}_4$, the result of Yeochon was 3 times higher than that of Mokpo in 1996, while the results of two regions were almost similar in 1997. The reason was that Yeochon was restricted area of the use of 0.5% sulfur B-C oil, controlled air pollution emission area, controlled semitotal amounts of air pollution emission area and partial operating of factories in 1997. There were no ions having high correlation with $H^+,;but;nss-SO^{2-}_4,;NO^-_3,;NH^+_4;and;nss-Ca^{2+}$ showed high correlation coefficient each other. It seems that these ions have little correlation with $H^+$ because they are washed out on binding state. Factor analysis showed that the first factor was complicated factor containing anthropogenic and soil resource, the second factor was sea-salt resource and the third factor was independent behavior of hydrogen at Mokpo. While, the first factor was complicated factor containing anthropogenic and sea-salt resource, the second factor was $Ca(NO_3)_2$ salt and the third factor was $NH^+_4;and;SO^{2-}_4$ synergied by resource and combination at Yeochon.

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향기요법(香氣療法)을 병행(幷行)한 소아비연(小兒鼻淵) 환자(患者) 17예(例)에 대한 임상적(臨床的) 연구(硏究) (Clinical Study in 17cases of patients for sinusitis(treated with aroma-therapy))

  • 한재경;김윤희;김연진
    • 대한한방소아과학회지
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    • 제14권2호
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    • pp.105-120
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    • 2000
  • Clinical studies were done treated with aroma-therapy in 17cases of patients for suffering sinusitis, who were treated at Dept. of Pediatrics in the Hospital of Daejon from March 2000 to November 2000. The results were as follows. 1. In distribution of sex & age, 70.6% was men and 29.4% women. 47.1% was between 5 and 7 years old, 35.3% within 5 years old. 2. In distribution of the period of the clinical history, 35.3% was over 6 momths, 29.4% of them between 6 and 12 months. 3. In the living environment the apartment was 58.5% and the residental zone is 29.4%. 4. The incurrence of season is winter, 47.0% next is change of season, 29.4%. 5. At night the symptom is the most common, 52.9%. In the morning is the next, 35.3%. 6. In the past history. tinnitus was the best at 38.4%. The otitis media. atopic dermatitis, convulsion disease is next at 15.4%. 7. In the family history. the allergic rhinitis or sinusitis is the most rich at 38.4% and tinnitus is next at 23.0%. 8. In distribution of the chief complain companied with sinusitis, 23.3% was nasal obstruction, each 18.4% of them nasal discharge, cough. 9. In distribution of the duration of therapy, 29.4% was over 6 months, 23.5% between 5-6 months. In distribution of the therapeutic method, 47.1% was treated with western and oriental medicine and 42.1% only oriental medical care. 10. In distribution of the period of improvement of symptom(by X-ray reading), 35.2% was between 2-3 momths, 29.4% between 1-2 months. 11. In emotional change, after aroma-therapy, 82.4% of them felt good. 12. After treatment, 70.6% was excellent, 17.6% was good.

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Planar Structural Requirement at 4-position of 1-Arylsulfonyl-4-phenyl-4,5-dihydro-2-imidazolones for their Cytotoxicity

  • Jung, Sang-Hun;Kwak, Suhk-Jun
    • Archives of Pharmacal Research
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    • 제20권3호
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    • pp.283-287
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    • 1997
  • In order to identify the structural requirement at 4-position of 1-arylsulfonyl-4-phenyl-4,5-dihydro-2-limidazolones 3 for their cytotoxicity, the corresponding 1-arylsulfonyl-4-cyclohexyl-4,5-dihydro-2-imidazolones 4 were synthesized and their in vitro cytotoxicity against human solid tumor cell lines were measured. Unlike compounds 3a-c, cyclohexyl analogues 4a-c do not show the cytotoxicity. This dramatic loss of activity of these analogues on the volume change with the bulkier cyclohexyl group indicates that the planar structure at 4-position of 1-arylsulfonyl-4-pheny-4,5-dihydro-2-limidazolones 3 is required for their activity as an important pharmacophoric moiety.

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Studies on the Viability of Frozen Removed Seminal Plasma by Saline(RSP-S) and Tris-buffer(RSP-T) Semen of Small Spcies Dogs

  • Kim, S. K.
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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    • pp.57-57
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    • 2001
  • This study was carried out to investigate the general characteristics such as volume, sperm concentration, sperm motility, sperm abnormality on whole semen, RSP-S and RSP-T semen and fractional semen of small size dogs, and the effect of temperature and preservation time and cryoproservation on motility of whole and RSP-S and RSP- T semen. Multiple ejaculates were collected from small dogs by the digital manipulation of penis. 1. The volume per ejaculate semen, sperm of concentration and motility and abnormal sperm rate of 1st fractional semen were 0.65±0.09㎖, 4.52±0.35×10/sup 6/ cells/㎖, 15.64±3.85% and 5.50±0.62%. Also, 2nd fractional semen were 1.25±0.20㎖, 3.35±0.48×10/sup 6/cells/㎖, 96.25±4.65% and 4.24±0.46%. And 3rd fractional semen were 1.45±0.21㎖, 3.85±0.52×10/sup 6/cell/㎖, 92.82±4.24% and 4.66±0.58%, respectively. 2. The sperm of concentration and motility and abnormal sperm rates of whole, RSP-S and RSP-T semen were 5.45±0.82×10/sup 6/ cells/㎖, 95.55±4.65%, 4.58±0.45% and 4.82±0.36×10/sup 6/cells/㎖, 90.10±3.42%, 6.48±0.68% and 4.55±0.45× 10/sup 6/cells/㎖, 93.25±3.85%, 4.82±0.58%, respectively. 3. The motility of whole, RSP-S and RSP-T semen were higher at 4℃ than at 38℃. When preservation temperature was at 4℃, survival rates of RSP-S and RSP-T sperm were 97.54%-6.25% at 1-72 hrs, 97.40%-5.62% at 1-100 hrs, respectively. 4. The survival rates of slow and rapid frozen 2nd fraction, RSP-S and RSP-T semen were 67.3±4.45%, 88.8±4.46% and 46.4±3.84%, 74.4±4.20%, respectively. Survival rates was significantly higher in frozen RSP-S and RSP-T semen than that in control group(8.5±2.12%).

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정방형 딤플을 가진 회전원주의 유동장 특성 (Flowfield Characteristics of a Rotating Circular Cylinder Having Square Dimpled Surface)

  • 노기덕;강명훈
    • 대한기계학회논문집B
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    • 제28권4호
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    • pp.486-492
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    • 2004
  • Flow patterns around a rotating circular cylinder having square dimpled surface were visualized by the hydrogen bubble technique at velocity ratios from a=0 to 4.8 and Reynolds number of Re=1.0${\times}$10$^4$. The wake region of the cylinder was reduced as the velocity ratios increase and was smaller than that of the smooth cylinder without dimples at the same velocity ratio. The hydrodynamic characteristics on the cylinder was investigated by measuring of lift and drag at velocity ratios from a=0 to 4.1 and Reynolds number from Re=1.2${\times}$10$^4$ to Re=2.0${\times}$10$^4$. As the velocity ratios increase, the average lift and drag coefficients were increased and at the same velocity ratio, the average lift was larger but the average drag was smaller than that of the smooth cylinder.