• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.1093-1100
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• 2013

Background: p53 gene variants i.e. 16 bp duplication in intron 3, Arg72Pro in exon 4 and G>A in intron 6 have been reported to modulate susceptibility to various malignancies. Therefore, the present study evaluated the role of these p53 polymorphisms in oral cancer susceptibility in a population from Gujarat, West India. Method: Genotype frequencies at the three p53 loci in 110 controls and 79 oral cancer cases were determined by the PCR-RFLP method. Results: Heterozygous individuals at exon 4 showed protection from developing oral cancer. Homozygous wild and heterozygous individuals at intron 3 and those heterozygous at exon 4 in combination appeared to be at lowered risk. Furthermore, carriers of the 16 bp duplication allele at intron 3, proline allele at exon 4 and G allele at intron 6 were protected from oral cancer development. Conclusion: p53 polymorphisms, especially Arg72Pro in exon 4 could significantly modify the risk of oral cancer development in Gujarat, West Indian population.

• 한국식품과학회지
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• 제41권3호
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• pp.251-257
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• 2009

머스크멜론의 수확 후 관리를 위한 저장온도 차이에 따른 품질변화를 알아보기 위하여 0, 4, $10{\pm}1^{\circ}C$ 에 25일간 저장하면서 품질차이를 확인하였다. 중량 감소율의 경우에는 저장 11일까지는 $0^{\circ}C$와 $4^{\circ}C$간의 큰 차이를 보이지 않았지만, 그 이후부터는 차이를 보이기 시작하면서 저장 25일에는 $0^{\circ}C$ 저장보다 4, $10^{\circ}C$에서 저장한 멜론이 약 2.9, 3.4배 정도 감소율이 큰 것으로 나타났다. 가용성고형물량의 경우에는 $10^{\circ}C$를 제외한 0, $4^{\circ}C$에서 저장기간 동안 증가하는 경향을 보였으며, 산도는 전체적으로 감소하는 경향을 보였으나 $4^{\circ}C$에서 저장한 것이 산도 감소율이 가장 적었다. 경도의 경우에는 $0^{\circ}C$에서 저장하는 것이 멜론의 조직연화를 가장 지연시키는 것으로 나타났으며, 소비자들이 멜론의 상품성을 판단할 때 중요한 꼭지의 경우에는 $4^{\circ}C$에서 저장한 것이 수분이 가장 오래 유지되는 것으로 나타났으며, 비타민 C의 경우에도 $4^{\circ}C$에서 저장하는 것이 손실률이 적었다. 무기질 함량은 저장 15일까지는 $10^{\circ}C$에서 저장한 것이 높았으나, 이후에는 $0^{\circ}C$에서 저장한 것이 높은 것으로 나타났다. 미생물 생장의 경우 저장 18일까지는 큰 차이를 보이지 않다가, 저장 25일 후에는 $0^{\circ}C$ 저장에 비하여 4, $10^{\circ}C$저장한 것이 1.31, 1.45배 미생물의 생장이 더 많은 것으로 나타났다. 관능적 품질에서 $10^{\circ}C$에 저장한 멜론은 저장 초기 단맛이 빠르게 증가하였다가 이후 크게 감소 할 뿐만 아니라 조직도 물러졌지만, $0^{\circ}C$와 $4^{\circ}C$ 저장한 멜론은 저장 22일 경과 후에도 상품성이 남아있는 것으로 나타났다. 이와 같은 결과를 종합해 볼 때, 저장 수명은 $0^{\circ}C$에서 보관한 것이 좋았다. 하지만 멜론의 경도 유지 부분만 보완된다면 산도, 꼭지의 수분함량, 비타민 C가 저장 시 우수했던 $4^{\circ}C$ 정도의 낮은 온도에서 저장하는 것이 수확 직후의 신선도와 품질을 가장 오래 유지시키는 것으로 판단되어진다.

• 생명과학회지
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• 제15권4호
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• pp.522-527
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• 2005

[ $4^{\circ}C$ ]와 $-2^{\circ}C$보관한 배최장근과 대퇴근 모두 저장기간이 경과함에 따라서 보수성은 감소하였고, 배최장근의 변화가 대퇴근의 변화보다 크게 나타났다 $4^{\circ}C$저장구보다 $-2^{\circ}C$저장한 사슴육이 낮은 TBARS가를 나타내어 저장기간 연장이 가능하였다. $4^{\circ}C$와 $-2^{\circ}C$에 저장한 사슴육의 VBN가는 저장 15일간 가식권에 있었고, 저장온도에 따른 차이는 크게 나타나지 않았다. 저장기간 경과에 따라서 배최장근과 대퇴근 모두 pH는 증가하는 경향이었고, $-2^{\circ}C$저장구가 $4^{\circ}C$저장구보다 낮은 pH를 나타내었다. 육의 연화는 $4^{\circ}C$에서는 두드러졌으나, $-2^{\circ}C$에서는 지연시키는 경향을 나타내어 $-2^{\circ}C$저장이 저장기간 연장에 효과가 있었다. $-2^{\circ}C$ 저장구가 $4^{\circ}C$저장구 보다는 자기가 어둡게 나타났고, 적색도는 $-2^{\circ}C$가 높게 나타났으며, 황색도는 저장기간 경과에 따라 $-2^{\circ}C$저장구의 변화가 크게 나타났다.

• Biomolecules & Therapeutics
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• 제5권2호
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• pp.133-149
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• 1997

4-week repeated dose toxicity of DA-5018, a new capsaicin analogue analgesic agent, was examined in 5D rats at dosage levels of 0,0.4,2, 10 and 50 mg/kg/day. DA-5018 was administered orally to 17 males and 17 females per group at doses of 0, 10 and 50 mg/kg and to 12 males and 12 females per group at doses of 0.4 and 2 mg/kg. After the administration period, 5 males and 5 females at the 0, 10 and 50 mg/kg were placed on withdrawal for 2 weeks. Treatment-related clinical signs were observed at 10 and 50 mg/kg. Clinical signs observed immediately after the administration of DA-5018 were grooming, sedation or depression, lacrimation, atacia, reddening of extremities and ears, ventral or lateral recumvincy, respiratory distress, cyanosis and convulsion. Delayed-type clinical signs including focal scabbing and depilation around nose were also observed 1 or 2 weeks after the start of administration of DA-5018. Only at the 50 mg/kg group, corneal opacities, reduced body weight gain (male) and death (male 6/17, female 3/17) were noted. In blood biochemical analysis, serum levels of glucose and triglyceride decreased at 10 and 50 mg/kg. In hematological examination, there were increases in the number of red blood cell, hemoglobin content and percent of hematocrit at 10 and 50 mg/kg. Pulmonary enlargement and hemorrhagic spot, focal scabbing and depilation around nose and corneal opacities were seen at the necropsy of the animals died during the dosing of DA-5018 50 mg/kg. Focal scabbing and depilation around nose were observed in the animals terminally necropsied at doses of 10 and 50 mg/kg. Histopathological examination revealed pulmonary hemorrhage, focal necrosis in the scabbed area, corneal necrosis, fibrosis and neovasculization in the stroma. At 0.4 and 2 mg/kg, there were no significant toxic changes attributable to the administration of DA-5018. In conclusion, target organs following to 4-week repeated dose of DA-5018 in the rat were determined to be lung, skin and eyes. Definite toxic dose and no-observed-adverse-effect-level (NOAEL) were estimated to be 50 and 2 mg/kg/day, respectively.

• Journal of Korean Neurosurgical Society
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• 제45권1호
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• pp.24-31
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• 2009

Objective : To investigate the effectiveness of radiographic parameters on segmental instability in the lumbar spine using Kinetic magnetic resonance imaging (MRI). Methods : Segmental motion, defined as excessive (more than 3 mm) translational motion from flexion to extension, was investigated in 309 subjects (927 segments) using Kinetic MRI. Radiographic parameters which can help indicate segmental instability include disc degeneration (DD), facet joint osteoarthritis (FJO), and ligament flavum hypertrophy (LFH). These three radiographic parameters were simultaneously evaluated, and the combinations corresponding to significant segmental instability at each level were determined. Results : The overall incidence of segmental instability was 10.5% at L3-L4, 16.5% at L4-L5, and 7.3% at L5-S1. DD and LFH at L3-L4 and FJO and LFH at L4-L5 were individually associated with segmental instability (p<0.05). At L4-L5, the following combinations had a higher incidence of segmental instability (p<0.05) when compared to other segments : (1) Grade IV DD with grade 3 FJO, (2) Grade 2 or 3 FJO with the presence of LFH, and (3) Grade IV DD with the presence of LFH. At L5-S1, the group with Grade III disc and Grade 3 FJO had a higher incidence of segmental instability than the group with Grade I or II DD and Grade 1 FJO. Conclusion : This study showed that the presences of either Grade IV DD or grade 3 FJO with LFH at L4-L5 were good indicators for segmental instability. Therefore, using these parameters simultaneously in patients with segmental instability would be useful for determining candidacy for surgical treatment.

• 한국보육지원학회지
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• 제13권2호
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• pp.21-37
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• 2017

Objective: The current study aimed at examining the mediation effects of children's self-esteem and self-control in learning between perceived academic achievement at $4^{th}$ grade and $6^{th}$ grade. This article proposes that perceived academic achievement boosts self-esteem and self-control in learning and both in turn, influence subsequent perceived academic achievement. We especially attempted to empirically prove that a serial multiple mediation of self-esteem and self-control in learning between the perceived academic achievement at two time points exists. Methods: We analyzed the longitudinal data of 1,881 children from the $4^{th}$ to the $6^{th}$ wave data of the '2010 Korea Children and Youth Panel Survey (KCYPS)' by means of a Hayes's PROCESS(2012) program. Results: The results revealed that perceived academic achievement at $4^{th}$ grade influenced children's self-esteem and self-control in learning at $5^{th}$ grade. Children' self-esteem and self-control in learning subsequently predicted perceived academic achievement at $6^{th}$ grade. Children's self-esteem significantly predicted self-control in learning supporting the hypothesis of serial multiple mediation. Conclusion/Implications: In conclusion, children's self-esteem and self-control in learning behaviors both mediated the association between perceived academic achievement at $4^{th}$ grade and at $6^{th}$ grade. The findings imply the importance of consideration of both psychosocial and behavioral aspects in understanding the academic performance during childhood.

• Journal of Chest Surgery
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• 제20권2호
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• pp.289-299
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• 1987

A total of 1,239 patients had cardiac valve replacement using 1,514 substitute valves at Seoul National University Hospital from 1968 to 1986. Of the total substitute vales, 84.9% were the glutaraldehyde-treated xenograft valves. Six hundred ninety-four patients who had 820 bioprosthetic tissue valves were studied for their clinical characteristics. They were a total and consecutive cases to the end of the study. Four hundred sixty-four patients had the lonescu-Shiley pericardial valves: MVR 291, AVR 66 and MVR+AVR 107; 163 had the Hancock porcine valves; 46 had the Angell-Shiley porcine valves; and 21 had the Carpentier-Edwards porcine valves. Five hundred forty patients underwent single valve replacement: MVR 460, AVR 76 and TVR 4; 154 had multiple valve replacement: MVR+AVR 141, MVR+TVR 12 and one triple valve replacement. Additional surgery was necessary in 22.3% of the cases. Operative mortality rate within 30 days of surgery was 6.77% for the total patients: 5.2% and 4.2% with MVR, 13.6% and 12.5% with AVR, and 7.5% and 7.4% with MVR+AVR using the lonescu and the Hancock valves respectively. A linealized annual late mortality rate was 2.56%/patient-year. Six hundred forty-three operative survivors were followed up for a total of 1482.7 patient-years [a mean 27.7 months], and the follow-up rate was 67.7%. The Idealized complication rates were: 2.02% emboli/patient-year, 0.94% bleeding/patient-year, 1.21% endocarditis/patient-year, and 3.84% overall valve failure/patient-year. A linealized rate of primary tissue failure was 0.87%/patient-year. Actuarial survival rates including the operative mortality were: 87.8*2.6%, 82.3*4.9% and 82.2*4.7% with MVR, AVR and MVR+AVR using the lonescu valves at 4 years after surgery respectively; and they were 88.0*4.1% with MVR at 8 years, 82.3*4.9% with AVR at 4 years and 84.9*7.0% with MVR+AVR at 6 years after surgery using the Hancock valves respectively. Probabilities of freedom from thromboembolism were 89.8*6.3% with MVR using the lonescu valves at postoperative 5 years and 89.2*3.8% with MVR using the Hancock valves at postoperative 7 years, and 93.3*3.9% with AVR using the lonescu valves at postoperative 5 years. None had embolic complication after AVR using the Hancock valves. Probabilities of freedom from valve failure [according to the Stanford criteria] were 81.0*7.1% with MVR using the lonescu valves at postoperative 4 years and 57.4*12.5% with MVR using the Hancock valves at postoperative 9 years. These clinical results prove the excellent antithrombogenicity of the glutaraldehyde-treated xenograft substitute valves and confirm the previously speculated rate of tissue failure. At the present situation, it may be concluded that there is a room for the further development of more durable bioprosthetic valves.

• 농업과학연구
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• 제40권4호
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• pp.297-303
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• 2013

This study was carried out to examine the effect of Red LED (660 nm) and fluorescent lamp for night break (NB) treatments of each 3 hours (22:30-01:30), 4 hours (22:00-02:00) and 5 hours (21:30-:02:30) per day for 53 days on flower bud initiation and growth in Chrysanthemum cv. 'Baekma' and cv. 'Jinba'. The days to flower budding after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (21.0 days) and 5 hr (20.5 days) NB, and it was shorter at Red LED 3 hr (14.2 days). The days to flowering after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (54.0 days), 5 hr (53.5 days) NB, and Red LED 5 hr (53.3 days), and it was shortest at Red LED 3 hr (50.2 days) NB treatment among all treatments. The days to flower budding after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (20.6 days) and was shorter at Red LED 3 hr (14.1 days) among all treatments. Similarly, the days to flowering after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (55.3 days) and was shortest at Red LED 3 hr (50.2 days) among all treatments. Therefore, inhibition of flower bud initiation was the most effective under fluorescent lamp 4 hr treatment. The length of cut flower of 'Baekma' was increased by fluorescent lamp 4 hr, 5 hr, and Red LED 5 hr, but of 'Jinba' was longer at LED 4 hr and 5 hr treatment. The weight of cut flower of 'Baekma' was heaviest at fluorescent lamp 5 hr treatment and was at Red LED 5hr treatment for 'Jinba' even though there was not statistically significant difference between 'Baekma' and 'Jinba'. Consequently, under fluorescent lamp 4 hr for night break was the most effective on flower bud initiation, flowering inhibition and cut-flower characteristics in 'Baekma' and 'Jinba'.

• 한국광물학회지
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• 제13권1호
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• pp.44-52
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• 2000

천연에서 발견되는 geversite (PtSb2), stumpflite (PtSb), insizwaite (PtBi2), unnamed PtBi 등의 광물에 대한 안정영역과 원소치환에 따른 고용체 존재를 규명하기 위해 백금-안티모니-비스머스 등 3성분계에 대한 합성실험적 연구를 실시하였다. 이번 연구에서 설정된 $600^{\circ}C$ 온도의 실험결과에 의하면, 등축정계의 geversite와 insizwaite 사이에 완전고용체가 형성되며, Sb를 치환하는 Bi의 함량에 따라 단위포 상수는 6.4415(0 at.%), 6.4361(15 at.%), 6.5204(30 at.%), 6.5411(51 at.%), 6.6261(70 at%), 6.6540(85 at%), 6.728$\AA$(100 at.%)로 증가함을 알 수 있었다. 육방정계인 stumpflite와 unnamed PtBi 사이에도 완전고용체가 형성되며, Sb를 치환하는 Bi의 함량이 증가함에 따라 a 단위포 상수의 크기는 4.1388(0 at.%), 4.2118(20 at.%), 4.2118(40 at.%), 4.2485(80 at.%), 4.3242$\AA$(100 at.%)등 연속적으로 증가하지만, c 단위포 상수는 각각 5.4902, 5.4799, 5.508, 5.4817, 5.5045$\AA$등 불규칙하게 변함을 알 수 있었다. 0~33.33 at.% Pt 영역에서의 상평형 관계는 액체가 Pt(Sb,Bi)2 고용체와 공존하고 있고, Sb가 많이 함유된 액체에서는 geversite+원소광물 안티모니+백금이 거의 함유되지 않은 액체와 공생하는 3-phase assemblage를 형성한다. 자연계에서는 geversite와 insizwaite 및 stumpflite와 unnamed PtBi 사이의 화학조성을 가지는 광물이 발견되고 있는데, 이들은 각각 독립적인 광물종이 아니라 위 광물들의 고용체에 속하는 것임을 알 수 있었다. 이들 광물을 명명하고 해석하는데 매우 세심한 주의가 필요함을 알 수 있었다. 또한 단위포 상수를 측정을 통해 해당 고용체 광물의 Sb↔Bi 치환 양을 추정할 수 있다는 점과 광물 공생관계를 통해 생성온도를 추정할 수 있다는 사실을 알 수 있었다.

• 한국대기환경학회지
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• 제8권3호
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• pp.191-197
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• 1992

In order to investigate the chemical components of acid precipitation at Kangwha near the Yellow Sea and Seoul in Korea, the precipitation samples were collected by wetonly precipitation sampler from February 1991 to January 1992, and pH, electric conductivity(E. C.) and major water-soluble ionic components were analyzed. Strong negative linear correlations were observed between the rainfall amount and the sum of major ionic components in $\mu eq/\ell$ at two sites. The sum of major ionic components also correlated negatively with rain intensity. The analytical results of precipitation samples at two sites were compared each other. Average values of volume-weighted pH were found to be 5.21 at Kangwha and 5.09 at Seoul. The cationic abundance($\mu eq/\ell$) in rainwater showed the general trend $NH_4^+ > Na^+ > Ca^{2+} > Mg^{2-+} > H^+ > K^+$ at Kangwah and $NH_4^+ > Ca^{2+} > Na^+ > H^+ > Mg^{2+} > K^+$ at Seoul. The anionic abundance showed the general trend $SO_4^{2-} > Cl^- > NO_3^-$ at Kangwha and $SO_4^{2-} > NO_3^- > Cl^-$ at Seoul. The concentrations of seasalt such as $Na^+ and Cl^-$ were higher at Kangwha than Seoul. The concentrations of $nss-SO_4^{2-}, nss-Cl^- and NO_3^-$ which are acid composition were higher at Seoul(96.3 $\mu eq/\ell$) than Kangwha(69.0 $\mu eq/\ell$). The contribution of seasalt to the composition of precipitation were higher at Kangwha(34.1%) than Seoul(15.7%). Ammonia and calcium species in rainwater at Kangwha and Seoul are interpreted to have 91% of neutralizing capacity of the original sulfuric and nitric acids. Provided that the precipitation acidity originates primarily from sulfate and nitrate, sulfate was found to contribute about 73-75% of the free precipitation acidity.