• Applied Biological Chemistry
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• v.40 no.6
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• pp.536-540
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• 1997

Volatile terpenoids of carrots were measured by the direct headspace sampling method(DHS) with gas chromatography as a study for the breeding of high quality carrots. Using this method, 7 terpenoids such as ${\alpha}-pinene$, ${\beta}-pinene$, ${\beta}-myrcene$, ${\alpha}-terpinene$, limonene, ${\gamma}-terpinene$, and terpinolene were clearly separated. However ${\alpha}-phellandrene$ was not clearly separated from ${\beta}-myrcene$. In addition to this, higher boiling point compounds such as terpinen-4-ol, bornyl acetate, and ${\alpha}-bisbolol$ were not found. The coefficients of $determination(r^2)$ for the 7 terpenoids were higher than 0.99 and the standard curves were highly significant. Four replicated samples using this method demonstrated great reproducibility; the coefficidnt of variation (C.V.) for ${\alpha}-pinene$, ${\beta}-pinene$, ${\beta}-myrcene$, limonene, ${\gamma}-terpinene$, terpinolene, and total terpenoids were 6.8, 6.8, 8.4, 7.1, 3.8, 10.1, 7.1%. Sixty five carrot cultivars breeded worldwide were evaluated for the 7 terpenoids and total terpenoids; the range for ${\alpha}-pinene$, ${\beta}-pinene$, ${\beta}-myrcene$, ${\alpha}-terpinene$, limonene, ${\gamma}-terpinene$, terpinolene, and total terpenoids were $0.28{\sim}2.48\;ppm$, $0.35{\sim}1.87\;ppm$, $0.56{\sim}1.51\;ppm$, 0 ppm, $0.59{\sim}1.84\;ppm$, $0.87{\sim}3.33\;ppm$, $5.15{\sim}35.81\;ppm$, and $9.07{\sim}42.30\;ppm$, respectively. Big differences in each terpenoid and total terpenoids were found among cultivars. On the total terpenoids of the 65 cultivars, 5 cultivars(7.7%) contained less than 10 ppm, 15 cultivars (23.1%) $10{\sim}11.99\;ppm$, 14 cultivars(21.5%) $12{\sim}13.99\;ppm$, 9 cultivars(13.8%) $14{\sim}15.99\;ppm$, 10 cultivars(15.4%) $16{\sim}17.99\;ppm$, 4 cultivars(6.2%) $18{\sim}19.99\;ppm$, 5 cultivars(7.7%) $20{\sim}29.99\;ppm$, and 3 cultivars(4.6%) were higher than 30 ppm. Generally, cultivars developed in Japan contained less total terpenoids than cultivars developed in Europe and America.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.282-288
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• 1996

Because of the high water content of the household garbage, it is difficult to compost it. Therefore, this study was conducted to investigate the possibility of using coal fly ash as humidity conditioner for the household garbage composting. The summarized results are as follows : 1. The maximum temperatures were $35^{\circ}C$ in spring, $47^{\circ}C$ in summer, and $43^{\circ}C$ in winter during the composting periods. 2. The mass was reduced to 70.5% after 60 days. The average volume reduced down to 74.7% after 60 days. 3. The seasonal variation of pH values showed a similar tendency and reached 8.5 after 60 days. 4. The water content was reduced at an early stage of composting and not much changed thereafter. It had 49.7% in spring, 33.9% in summer, and 56.5% in winter after 60 days. Ash contents were not much changed during the composting periods. 5. The contents of inorganic compounds were in the range of $0.06{\sim}4.03%$ $P_2O_5$, $0.01{\sim}2.87%$ CaO, $0.18{\sim}1.43%$ MgO, and $0.39{\sim}2.03%$ $K_2O$. Heavy metal contents were in the range of $ND{\sim}14.08$ Hg ${\mu}g/kg$, $ND{\sim}0.80$ Cd mg/kg, $4.99{\sim}28.95$ Cu mg/kg, $ND{\sim}242.62$ Cr mg/kg, $ND{\sim}20.24$ Pb mg/kg, and $ND{\sim}59.87$ Zn mg/kg.

• Korean Journal of Environmental Agriculture
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• v.23 no.1
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• pp.15-21
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• 2004

Objectives of this study were to monitor the distribution of heavy metals, to compare extractable heavy metal with total content and to investigate the relationships between soil physico-chemical properties and heavy metals in orchard soil. Sampling sites were 48 in Gyeonggi, 36 in Gangwon, 36 in Chungbuk, and 44 in Chungnam, Soils were collected farm form two depths, 0 to 20 and 20 to 40 cm (here after referred to as upper and lower layers) from March to May in 1998. Total contents of heavy metal in soils were analyzed by ICP-OES after acid digestion ($HNO_3$:HCl:$H_2O_2$) whereas extractable contents were measured after successive extraction of 0.1N-HCl, 0.05 M-EDTA, and 0.005 M-DTPA. Mercury was analysed by mercury atomizer. The average contents of Cd Cu, and Pb in the extractant with 0.1N-HCl at upper layer were 0.080, 4.23, and 3.42 mg/kg, respectively. As content in the extractant with 1N-HCl was 0.44 mg/kg, and total contents of Zn, Ni and Hg were 78.9, 16.1, and 0.052 mg/kg, respectively. The ratios of concentrations of heavy metals to threshold values (Cd 1.5, Cu 50, Pb 100, Zn 300, Ni 40, Hg 4 mg/ke in Soil Environmental Conservation Act in Korea (2001) were low in the range of $1/2.5{\sim}1/76.9$ in orchard soils. The ratios of extractable heavy metal to total content ranged $5.4{\sim}9.21%$ for Cd, $27.9{\sim}47.8%$ for Cu, $12.6{\sim}21.8$% for Pb, $15.8{\sim}20.3%$ for Zn, $5.3{\sim}6.3%$ for Ni, and $0.7{\sim}3.6%$ for Zn, respectively. Cu and Pb contents in 0.05 M-EDTA extractable solution were higher than those in the other extractable solution. Total contents of Cd, Ni and Ni in soils were negatively correlated with sand content but positively correlated with silt and clay contents. Ratios of extractable heavy metal to total content were negatively correlated with clay content but ai and Ni contents were positively correlated with soil pH, organic matter, and available phosphorous. Therefore, the orchard soil was safe because the heavy metal contents of orchard soil were very low as compared to its threshold value in the Soil Environmental Conservation Act. However, it need to consider the input of agricultural materials to the agricultural land for farming practices for assessment of heavy metals.

• Journal of the Korean Society of Food Science and Nutrition
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• v.9 no.1
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• pp.51-58
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• 1980

Sixteen species of algae (4 species of green algae, 5 species of brown algae and 7 species of red algae) were collected from the coast of Chungmu, Gyeongnam, from June to October, 1976. The content of minerals in algae were analyzed by Atometic Absorption Spectrophotometer. The results were as followed 1) The content of Iron and Nickel in green algae were abundant, and those of Lead, Nickel and Manganese in brown algae were low. 2) The content of Cadmium were in the range of 0.58-1.04ppm (average: 0.85ppm) in green algae, 0.32-2.10ppm (average: 1.08ppm) in brown algae and 0.54-1.70ppm (average: 1.04ppm) in red algae. The content of Cadmium were in the range of 0.3-0.6ppm in laver, Porphyra tenera, sea mustard, Undaria pinnatifida, and tangle, Laminaria japonica, but its content was lower than those expected. 3) The content of Lead were in the range of 0.67-1.40ppm (average: 1.03ppm) in green algae, 0.60-1.00ppm (average: 0.82ppm) in brown algae, 0.56-2.40ppm (average: 1.28ppm) in red algae and its content in algae were lower than in fish and shellfish. 4) The content of Copper were in the range of 10.8-24.2ppm (average: 18.95ppm) in green algae, 7.4-24.6ppm (average: 18.16ppm) in brown algae, 6.4-31.2ppm (average: 19.94ppm) in red algae and those content were considerably abundant except for some algae. 5) The content of Nickel were in the range of 5.4-16.6ppm (average: 9.1ppm) in green algae, 1.0-4.4ppm (average: 2.32ppm) in brown algae and 0.7-4.6ppm (average: 2.59ppm) in red algae. 6) The content of Iron were in the range of 686.4-1159.0ppm (average: 916.5ppm) in green algae, 131.0-499.2ppm (average: 310.16ppm) in brown algae and 156.0-530.4ppm (average: 248.2ppm) in red algae. Especially, that of Iron in green algae showed higher value than in any other. 7) The content of Manganese were in the range of 48-221ppm (average: 157.25ppm) in green algae, 12-65ppm (average: 41ppm) in brown algae and 72-162ppm (average: 121ppm) in red algae. Especially, that of Manganese in brown algae showed lower value than in any other. 8) The content of Zinc were in the range of 191.3-451.1ppm (average: 290.05ppm) in green algae, 89.9-374.2ppm (average: 202.64ppm) in brown algae and 106.4-281.4ppm (average: 188.93ppm) in red algae. 9) The content of Magnesium were in the range of 0.48-1.83% (average: 1.27%) in green algae, 1.04-1.71% (average: 1.21%) in brown algae and 0.42-1.24% (average: 0.097%) in red algae. 10) The content of Fluorine were in the range of 29.2-92.7ppm (average: 53.03ppm) in green algae, 33.3-43.5ppm (average: 39.18ppm) in brown algae and 32.4-59.0ppm (average: 44.84ppm) in red algae.

• Korean Journal of Ecology and Environment
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• v.34 no.3 s.95
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• pp.199-205
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• 2001

This study was investigated to the assessment of organic pollution using attached diatom assemblages in the Shinchon stream on April, June, September and December 1999. Diatoms were identified total 74 taxa which composed of 68 species, 4 varieties and 2 unidentified species. The Centrales were 5 taxa and Pennales were 69 taxa. Dominant species were Navicula subminuscula, Navicula saprophila and Nitzschia palea which were saprophilous taxa to organic pollution. N. palea was dominant species at st. $1{\sim}3$ on April, June and September and N. subminuscula was recorded as dominant species at st. 4 on June, September and December. N. saprophila was dominated only at station 4 on April. DAIpo values were ranged $0.4{\sim}15.3$ at all stations from April to December and the water qualities were polysaprobic states except to ${\alpha}-mesosaprobic$ state at st. 1 on June. If Navicula sp. was identified as saprophilous taxa, DAIpo value of this station will be 3.5 and water quality of the station will be polysaprobic state. This stream was assessed the water qualities of polysaprobic states.

• Korean Journal of Environmental Biology
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• v.24 no.1 s.61
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• pp.53-59
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• 2006

This study was carried out to obtain the biological studies on aquaculture fundamental data for the resources annexation of Miichthys miiuy in terms of the characteristics of the spawning and effect of salinity. The adults spawners in 5 years were TL $72.3\sim89.6\;cm$, BW $3,736\sim8,818\;g$ in female (n=39), TL $47.1\sim81.2\;cm$, BW $716.6\sim6,853\;g$ in male (n=24). The adults size which were suitable for a stable egg collection were $97.9\sim110.2\;cm$ in total length, $9,657\sim13,200\;g$ in body weight. Each egg contained $1\sim5$ oil globules. Also, the highest hatching rate was 96.7% at the one having an oil globules. The highest hatching rate was 87.0% at 30.0 ppt. The fastest time required from fertilization to hatching was 17 hours 24 minutes at 28.0 ppt.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.771-777
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• 1999

The structural function of three egg membrane layers and cuticle layer, and the effectiveness of 5 film coatings (chitosan, starch, gelatin, dextrin, mineral oil) on the prevention of Salmonella enteritidis penetration was investigated using confocal scanning laser microscopy (CSLM). Diameters of outer membrane fibers, inner membrane fibers and limiting membrane particles in eggshell were $1.5{\sim}7.2$, $0.8{\sim}2.0$ and $0.1{\sim}1.4\;{\mu}m$, respectively and average thicknesses were 10.0, 3.5, $3.6\;{\mu}m$, respectively. Average thickness of cuticle layer was $6.0\;{\mu}m$ and cuticle layer covered $40{\sim}80%$ of total eggshell surface. Average coating films thickness for chitosan, starch, gelatin, dextrin and mineral oil were 2.2, 2.5, 3.9, 3.6 and $5.0\;{\mu}m$, respectively. After immersion process eggshell surface was almost completely covered by coating films. Chitosan coating was most effective among 5 film coatings in inhibiting growth of Salmonella enteritidis. Penetration process of Salmonella enteritidis through eggshell was investigated by multicolor imaging using CSLM and plate counting. Cuticle layer was the most important structure in blocking the penetration. Among 5 film coatings, chitosan showed the best and similar effectiveness with cuticle layer.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.2
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• pp.81-85
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• 2007

Early growth of chum salmon was investigated in terms of rearing pond condition. The number of fingerling showed big differences by rearing pond $1,003,000{\sim}1,865,000$. The fork length of fingerlings ranged from $3.20{\sim}3.78cm$ and body weight ranged $0.34{\sim}0.49g$. Daily temperature of rearing water in January to March was $6{\sim}8^{\circ}C$, which is inadequate for salmon growth. pH was $6.0{\sim}6.3$ in early and mid February, 6.5 in late February and $6.5{\sim}7.0$ in March. Dissolved oxygen concentration was lowest from 8 a.m. to 6 p.m. and increased after 6 p.m. The maximum value was seen from 9 p.m. to 6 a.m. The mortality of fingerlings was 5 times higher in the daytime than at night because of dissolved oxygen. Growth of fingerlings was affected by daily ration, concentration of dissolved oxygen and rearing temperature.

• Korean Journal of Food Science and Technology
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• v.25 no.5
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• pp.449-455
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• 1993

Vibrio vulnificus is a recentry recognized halophilic organism that may cause human infections. Patients infected with Vibrio vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. In this studies 314 samples were collected from 9 sites of Chonnam coastal area, from April to Jun, 1993. Isolation rates of V. vulnificus were 7.1% in total samples, 33.3% in sea water, 55.5% in sediment, 1.7% in fish and shellfish, 6.3% in kitchen environment. In each areas of Chunnam V. vunificus were isolated from 3.6% to 11.4%. The isolation rates of V. vulnificus was correlated positively with organic matter and COD in sea warer and sediment. In sea water, Microorganism population were $4.5{\times}10^3{\sim}3.5{\times}10^5\;CFU/ml$, Vibrio strains were $1.8{\times}10^l4.6{\times}10^3\;CFU/ml$, Coliform bacteria were $1.9{\times}10^1{\times}3.7{\times}10^2\;CFU/100ml$. In sediment, Microorganism population were $4.8{\times}10^3{\sim}5.2{\times}10^5\;CFU/ml$. Vibrio strains were $1.9{\times}10^2{\sim}8.4{\times}10^4\;CFU/ml$, Coliform bacteria were $7.2{\times}10^1{\sim}9.9{\times}10^2\;CFU/100ml$.

• Korean Journal of Environmental Agriculture
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• v.15 no.2
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• pp.179-187
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• 1996

This study was conducted to investigate the possibility of composting of household garbage. The composter with the double layer walls was operated for 60 days in each season. The following results were obtained at the end of the experiment, if the time was not specified. 1) The maximum temperature was $31^{\circ}C$ in spring, $36^{\circ}C$ in summer and $50^{\circ}C$ in winter. 2) The mass was reduced to an average of 58.5%. 3) pH values of the compost were 8.21 in spring, 8.29 in summer and 7.94 in winter. 4) The ash contents were 55.8% in spring, 57% in summer and 73.8% in winter. 5) The nitrogen contents were in the range of $0.2{\sim}5.8%$. Its values were the highest in winter and the lowest in summer. 6) Inorganic contents of the compost were in the range of : $P_2O_5$ ; $1.5{\sim}4.41%$, $K_2O$ ; $0.02{\sim}1.31%$, CaO $0.13{\sim}1.68%$ and MgO $0.05{\sim}1.22%$. 7) Heavy metal contents of the compost were in the range of : Zn ; $13{\sim}89mg/kg$, Cu ; $4{\sim}62mg/kg$, Cd ; $1{\sim}21mg/kg$, Pb ; $N.D.{\sim}97mg/kg$, Cr ; $N.D.{\sim}37$ and Hg ; $N.D.{\sim}1.38mg/kg$.