• Title/Summary/Keyword: ${\gamma}-irradiation$

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Effect of Irradiation of Red Radish Seeds on the Seed Viability and Functional Properties of Sprouts

  • Waje, Catherine K.;Park, Ju-Hwan;Kim, Gui-Ran;Kim, Yu-Ri;Han, Bum-Soo;Lee, Yeon-Kyung;Moon, Kwang-Deog;Kwon, Joong-Ho
    • Food Science and Biotechnology
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    • v.18 no.2
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    • pp.390-395
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    • 2009
  • Red radish seeds were irradiated at doses up to 8 kGy using electron beam (e-beam) and gamma ray ($\gamma$-ray). The seed viability and functional properties (carotenoid, chlorophyll, ascorbic acid, and total phenol) of sprouts grown from these irradiated seeds were evaluated. High germination percentage ($\geq$97%) was observed in seeds irradiated at $\leq$5 kGy, but the yield ratio and sprout length significantly decreased with increased irradiation dose. Irradiation at $\geq$6 kGy resulted in curling of the sprout roots. Sprouting enhanced the functional properties of red radish seeds as indicated by the increased carotenoid, chlorophyll, ascorbic acid, and total phenol contents during germination. However, radiation treatment hampered the growth of seeds resulting in underdeveloped sprouts with decreased carotenoid, chlorophyll, ascorbic acid, and total phenol contents. In general, e-beam and $\gamma$-ray irradiation of red radish seeds showed similar effects on the seed viability and functional properties of sprouts. Postharvest storage reduced the functional quality of sprouts.

Changes in Physico-Chemical Properties of Salted Sea Mustard (Undaria Pinnatifida) by Gamma Irradiation (염장미역의 감마선 조사에 따른 이화확적 특성 변화)

  • 변명우;권중호;이수정;남상명;조한옥
    • Journal of Food Hygiene and Safety
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    • v.6 no.3
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    • pp.165-169
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    • 1991
  • The effect of gamma irradiation on physico-chemical properties of salted sea mustard (Undaria pinnatifida) was investigated. Chlorophyll and carotenoid pigment of salted sea mustard were partially decreased by irradiation. However there was no significant difference in the retention rate of pigment between control and 2 kGy-irradiated samples after six months of storage at around $10^{\circ}C$, ranging values of 74 to 77% in chlorophyll and 54 to 56% in carotenoid. Total organic acids and volatile acids associated with the organoleptic quality of sea mustard increased in the samples of lower salt concentrations and of higher storage temperatures. The softening of sample tissue by irradiation was shown to be correlated with the extraction properties of alginic acid.

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Application of Gamma Irradiation for the Reduction of Residual Nitrite and Nitrosamine in Meat Products

  • Ahn, Hyun-Joo;Kim, Jae-Hun;Lee, Ju-Woon;Jo, Cheorun;Byun, Myung-Woo
    • Food Science of Animal Resources
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    • v.24 no.4
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    • pp.367-372
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    • 2004
  • Nitrite, a curing agent of meat products, is precursors of carcinogenic N-nitrosamines during processing of meat products or under human stomach conditions as well as having its own toxicity. Some researches have been conducted to evaluate the effects of ionizing radiation on the reduction of residual nitrite and N-nitrosamines in an aqueous model system and cured meat products with different packaging methods during storage. These results showed that the gamma irradiation was effective in reducing the residual nitrite and N-nitrosamines in an aqueous model system as well as meat products. Especially, irradiation combined with vacuum or modified atmosphere packaging was more effective in nitrite and N-nitrosamines reduction than aerobic packaging during storage. The objective of this review is to introduce the irradiation technology for the application of reducing the residual nitrite and N-nitrosamine contents in meat products.

Synthesis and Biocompatibility of PVA/NaCMC Hydrogels Crosslinked by Cyclic Freezing/thawing and Subsequent Gamma-ray Irradiation

  • Shin, Ji-Yeon;Jeong, Heeseok;Lee, Deuk Yong
    • Journal of Biomedical Engineering Research
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    • v.39 no.4
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    • pp.161-167
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    • 2018
  • Polyvinyl alcohol/sodium carboxymethyl cellulose (PVA/NaCMC) hydrogels were prepared by physical crosslinking (cyclic freezing/thawing) and gamma (${\gamma}$)-ray irradiation to evaluate the effect of NaCMC concentration (2~8 wt%) on the mechanical properties and the biocompatibility of the PVA/NaCMC hydrogels. The swelling rate of PVA/NaCMC hydrogels regardless of irradiation rose with increasing NaCMC content from 2 wt% to 8 wt%, while the gelation rate was the reverse. As the NaCMC content increased from 2 wt% to 6 wt%, the compressive strength of the hydrogels increased dramatically from $8.5{\pm}2.0kPa$ to $52.7{\pm}2.5kPa$ before irradiation and from $13.5{\pm}2.9kPa$ to $65.5{\pm}8.7kPa$ after irradiation. When 8 wt% NaCMC was added afterwards, the compressive strength decreased however. The irradiated PVA/NaCMC hydrogels containing 6 wt% NaCMC exhibited the tailored properties of the swelling rate of $118{\pm}3.7%$, the gelation rate of $71.4{\pm}1.3%$, the strength of $65.5{\pm}8.7kPa$, respectively, and no cytotoxicity was observed.

Enzymatic Degradation of Poly(${\gamma}$-glutamic acid) Hydrogel Prepared by ${\gamma}$-Ray Irradiation

  • Hara, Toshio;Choi, Seong-Hyun;Choi, Woo-Young
    • Journal of Microbiology and Biotechnology
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    • v.11 no.2
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    • pp.342-345
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    • 2001
  • A bacterial strain PH-4, which produces an enzyme catalyzing the degradation of crosslinked poly(${\gamma}$-glutamic acid) hydrogels, was isolated and identified as a Flavobacterium sp. The enzyme was obtained by the sonication of the bacterial cells preincubated in a Bouillon medium with shaking, without adding of poly(${\gamma}$-glutamic acid) as an inducer. The products of the hydrogel degraded by the crude enzyme agreed closely with the depolymerized materials in SDS-polyacrylamide gel electrophoresis using methylene blue staining, and with a glutamic acid monomer on thin-layer chromatography, thereby suggesting that strain PH-4 produced a kind of exohydrolase.

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Anti-oxidative Activities Gamma Irradiated Shell of Persicae semen (감마선을 조사한 桃仁皮(도인피)의 항산화활성)

  • Cho, Woo-A
    • The Korea Journal of Herbology
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    • v.24 no.4
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    • pp.95-99
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    • 2009
  • Objectives : This study were carried out to investigate the anti-oxidative activities of $\gamma$-SPE(gamma irradiated shell of Persicae semen extract with ethanol). Methods : Anti-oxidative was measured by DPPH free radical scavenging activities, nitric oxide radical scavenging activities, SOD-like activities, xanthine oxidase radical scavenging activities, nitrite scavenging activities and tyrosinase inhibition activities. Results : Effects of free radial scavenging was increased by irradiation. Tyrosinase inhibition activities was decreased by $\gamma$-SPE. Conclusions : Results suggested that $\gamma$-SPE have a potential as a new anti-oxidative material.

Confirmatory test of gamma irradiation against the larvae and pupae of Helicoverpa assulta (Lepidoptera: Noctuidae) in paprika

  • Park, Jeong Sun;Jeong, Su Yeon;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.31 no.2
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    • pp.103-106
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    • 2015
  • The oriental tobacco budworm, Helicoverpa assulta (Lepidoptera: Noctuidae), is classified as a quarantine pest that must be controlled for the exportation of Korean paprika to the USA. We performed a confirmatory test of gamma irradiation against the last-instar larvae and pupae of H. assulta in paprika. Previous, small-scale gamma irradiation at a dose of 100 Gy to the last-instar larvae allowed emergence of adults, though they were abnormal, but 200 Gy prevented adult emergence completely. For pupae, irradiation of 5- to 6-day-old pupae with 300 Gy prevented normal emergence completely. To gather confirmatory data applicable to phytosanitary quarantine regulations, larvae and pupae were placed inside paprika in a box and were irradiated with 200 Gy and 300 Gy, respectively. After irradiation with 200 Gy (measured doses 170-199 Gy) of 2,186 individuals of the last-instar larvae, 10.84% survived, but either formed abnormal pupae (7.57%) or died during the pupal stage (3.27%), resulting in no emergence of normal adults. For pupae, the dose of 300 Gy (measured doses 276-319 Gy) given to 1,200 pupae allowed 10.75% to survive and 9.17% to emerge with deformity. However, 1.58% of irradiated pupae emerged normally, requiring an increased dose for complete prevention of normal emergence. Subsequently, an increased dose of 400 Gy (measured doses 340-402 Gy) to 1,005 pupae allowed 88.35% to emerge, but all emerged with deformity. Thus, irradiation treatment with a minimum dose of 400 Gy will provide quarantine security for all premature H. assulta in exported paprika.

Screening of Radio-resistant Lactic Acid Bacteria

  • Hwang, E-Nam;Kang, Sang-Mo;Kim, Jae-Kyung;Lee, Ju-Woon;Park, Jong-Heum
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.335-340
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    • 2013
  • This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pediocuccos pentosaceus - were selected and subcultuted twice. The LAB was then further cultured for 3 d at $37^{\circ}C$ to reach 7-10 Log colony-forming units (CFU)/mL prior to irradiation and immediately exposed to gamma rays or electron beams with absorbed doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Gamma irradiation gradually decreased the number of the tested viable LAB, and the effect was irradiation dose dependent. A similar effect was found in electron beam-irradiated LAB. Radiation sensitivity of LAB was calculated as $D_{10}$ values, which ranged from 0.26 kGy to 0.9 kGy and 0.5 kGy to 1.44 kGy with exposure to gamma and electron beam irradiation, respectively, in all tested LAB. L. acidophilus was the most resistant to gamma and electron beam irradiation, with $D_{10}$ values of 0.9 kGy and 1.44 kGy, respectively. These results suggest that L. acidophilus might be suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.

Effect of Gamma Irradiation and Post-Irradaition Treatment of Preservatives on the Cut Flower Longevity of Rose and Mum. (감마선 조사와 조사 후 보존제 처리가 절화 장미 및 국화의 수명에 미치는 영향)

  • 박인환;정연승;이월순;권중호;변명우
    • Food Science and Preservation
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    • v.6 no.3
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    • pp.286-291
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    • 1999
  • This study was carried out to determine the effect of dose and timing of gamma irradiation and post-irradiation treatment with some preservatives of cut flowers on their longevity of rose and chrysanthemum. Cut roses which were soaked in 2% sugar solution persist to 0.75 kGy irradiation treatments. In post-treatments with some preservative solutions, the mixture group of 2% sucrose,2000m silver nitrate and 3.0% crysal were available in flower quality, but the 200 ppm silver nitrate solution treatment showed serious blackening of floral stems and resulted On negative effect in flower quality. Effect of gamma irradiation and all preservative solutions on the cut mum longevity were showed almost same tendency with rose cut flower longevity experiments. The 2% sugar dipping solution in mum cut flower showed the most superior preservative effect compared with the my other preservative solutions tested. Also non-irradiated treatment extended to flower up to complete wilt.

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Development of Modified Starch by Gamma Irradiation (감마선을 이용한 변성전분의 개발)

  • Kang, Il-Jun;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.514-520
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    • 1996
  • The purpose of this study was to develop the production technology of modified starch. Corn starches were gamma irradiated at 0-110 kGy and the effect of irradiation dose levels on the physicochemical properties of corn starches were investigated. Blue value linearly decreased, while alkali number and solubility markedly increased as irradiation dose levels were increased. The optical transmittance increased as applied irradiation dose levels were increased in the temperature range of $65-95^{\circ}C.$ Water binding capacity and swelling power showed maximum value at 30 and 10 kGy, respectively and they tended to decrease thereafter. Gelatinization viscosity of the gamma irradiated starch considerably decreased as compared to that of the non-irradiated starch. Irradiation at 110kGy resulted in a marked reduction of peak viscosity and cooling viscosity at $30^{\circ}C$ by 100 and 300 times, respectively. The physicochemical properties of corn starch irradiated at 30 kGy were similar to those of commercial acid-modified starch, while those of corn starch irradiated at 100 kGy were similar to those if oxidized starch.

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