• 한국환경보건학회지
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• 제8권1호
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• pp.1-11
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• 1982

A study on the water pollution of Gumho river by the relationship between physio-chemical conditions and water quality level by phytoplankton was examined at 7 sampling positions during the period from Aug. 1 to Nov. 30, 1981. Examination of physio-chemicat water analysis such as temperature, pH, DO, BOD, and biological analysis are as follows: 1. pH was in the range of 6.6-7.3. 2. At all positions DO was0.5-11.9 mg/l. But at Shinchun bridge and Gangchang was 0.5-3.9 mg/l. 3. BOD was in the range of 3.4-29.2 mg/l. Banyawol, Dongchon and Gumdan was shown good condition. But at Shinchun bridge was 21.1-29.2 mg/l. 4. The plankton identification in this study period showed, Cyanophyceae is 7 genera 13 species, Bacillariophyceae is 11 genera 32 species, and Chlorophceae is 17 genera 27 species: total 35 genera 72 species. 5. In the point of phytoplankton classification, upper stream of Banyawol, Dongchon and Gumdan which BOD was 3.4-8.7 mg/l, dominant phytoplanktons were Synedra ulna, Ulothrix sp., Oscillatoria sp. and Frusturia rhomboides. At Shinchun bridge which BOD was 21.1-29.2 mg/l, Microcystis aeruginosa, Closterium acerosum and Oscillatoria sp were found a small. At 3rd gongdan which BOD was 9.2-12.5 mg/l, dominant species were Synedra ulna, Hormidium sp and Actinastrum hantzschii. At Paldal which BOD was 7.8-9.2 mg/l, dominant species were Nitzschia palea, Synedra ulna and Scenedesmus bijuga. At Gangchang of down stream which BOD was 6.9-9.2 mg/l, dominant phytoplanktons were Closterium acerosum, Microcystis aeruginosa and Actnastrum hantzschii. 6. The results of biological water analysis by saprobic system were as follows: Banyawol was from oligosaprobic to $\beta$-mesosaprobic, Dongchon and Gumdan was from $\beta$-mesosaprobic to $\beta$-mesosaprobic, Shinchun bridge was polysaprobic, 3rd gongdan was from $\alpha$-mesosaprobic to $\beta$-polysaprobic, Paldal was $\beta$-polysaprobic and Gangchang was $\alpha$-polysaprobic.

• 한국식품영양과학회지
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• 제16권2호
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• pp.128-135
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• 1987

고온성 cellulose 분해이용세균인 Herpetosiphon geysericola CUM 317 균주가 생성하는 ${\alpha}-amylase$, ${\beta}-amylase$ 및 glucoamylase를 황산암모늄 염석, DEAE-cellulose chromatography, CM-cellulose chromatography 방법으로 각각 부분정제하였다. 이들 ${\alpha}-amylase$, ${\beta}-amylase$ 및 glucoamylase의 감자 전분에 대한 Km치는 $2.31mg/m{\ell}$, $7.69mg/m{\ell}$ 및 $8.33mg/m{\ell}$였으며, 각 분자량은 84,000 dalton, 76,000 dalton 및 80,000 dalton의 크기로 나타났다.

• Journal of Radiation Protection and Research
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• 제24권1호
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• pp.39-43
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• 1999

최근 개발된 감도가 좋은 LiF:Mg,Cu,Na,Si TL물결을 Teflon과 혼합한 후, 않은 디스크 형태로 압축 성형한 얇은 LiF:Mg,Cu,Na,Si Teflon 검출기를 제작하고 베타선 검출기로서의 TL 반응특성을 연구하였다. 한국원자력연구소의 $^{147}Pm,\;^{204}Tl$ 및 $^{90}Sr/^{90}Y$ 베타선원을 이용하여 두께 $2mg/cm^2$인 Kapton 박막을 덮은 상태로 베타선을 조사하였다. 제작한 않은 LiF:Mg,Cu,Na,Si 검출기들의 $^{137}Cs$에 대한 batch 균질성은 4.7%, 베타선에 대한 선량의존성은 0.1 mGy에서 100 Gy까지 선형성을 나타내고, 에너지의존성은 $^{147}Pm,\;^{204}Tl$ 및 $^{90}Sr/^{90}Y$ 베타선에 대해 각각 0.46, 1.09 및 1.06 이었다. 그리고 베타선에 대한 방향의존성은 $0.93{\pm}0.03\;(^{147}Pm),\;0.94{\pm}0.04\;(^{204}Tl)$ 및 $0.92{\pm}0.05\;(^{90}Sr/^{90}Y)$으로, 이들 얇은 LiF:Mg,Cu,Na,Si 검출기들에 대한 TL 반응특성의 결과는 국제표준기구(ISO)의 베타선량계 기준을 잘 만족하였다.

• Mycobiology
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• 제41권3호
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• pp.159-163
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• 2013

A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high ${\beta}$-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and ${\beta}$-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in ${\beta}$-glucan productivity by producing 0.254 and 0.236 mg soluble ${\beta}$-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble ${\beta}$-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.

• 생명과학회지
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• 제24권2호
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• pp.128-136
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• 2014

알긴산은 ${\alpha}$-L-guluronic acid와 ${\beta}$-D-mannuronic acid가 (1-4) 결합한 선형 산성다당류이다. 알긴산은 다양한 알긴산 분해효소들에 의하여 분해되는데 ${\beta}$-제거반응으로 비환원 말단에 이중결합이 있는 불포화 우론산 올리고머가 생산된다. 본 연구실에서는 이전에 Stenotrophomonas maltophilia KJ-2로부터 새로운 구조를 가진 polyMG lyase를 재조합하였다. KJ-2 polyMG lyase의 단백질구조를 예측하기 위하여 상동성 모델링을 한 결과 Azotobacter vinelandii가 생산하는 세 종류의 polyMG lyase들이 모두 PL7 family에 속하는 반면 KJ-2 polyMG lyase는 PL6 family에 속하였다. 또한 $^1H$-NMR spectra를 분석한 결과 polyMG lyase는 M-${\beta}$(1-4)-G 당쇄결합을 분해하고 G-${\alpha}$(1-4)-M 결합은 거의 분해하지 못하는 것으로 나타났다. 예측된 polyMG lyase 모델을 기초로 하여 14군데 아미노산 잔기를 선택하였으며 17개의 돌연변이체를 만들어 알긴산 분해효소의 활성을 측정하였다. Lys220Ala, Arg241Ala, Arg241Lys및 Arg265Ala 돌연변이체들은 완전히 알긴산 분해효소의 활성을 잃었으며 Arg155Ala, Gly303Glu 및 Tyr304Phe 돌연변이체들의 분해활성은 19.1-39.3%까지 감소하였다. 이러한 결과들로부터 Arg155, Lys220, Arg241, Arg265, Gly303 및 Tyr304 들은 알긴산 분해효소의 촉매활성과 기질결합에 중요한 잔기들임을 알 수 있다.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.212-217
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• 1997

A strain of Bacillus sp. WS-14 was isolated from soil. Medium optimization for ${\beta}-mannanase$ production by Bacillus sp. WS-14 was performed. Effect of various carbon sources on ${\beta}-mannanase$ production was investigated and locust bean gum was the most effective for ${\beta}-mannanase$ production. ${\beta}-mannanase$ activity and cell growth increased with increasing the concentration of locust bean gum, however, the amounts were not significant. Among nitrogen sources, soytone was the most effective for ${\beta}-mannanase$ production. Inorganic compounds such as $KH_2PO_4,\;NaCl\;Na_2CO_3\;and\;MgSO_4{\cdot}7H_2O\;on\;{\beta}-mannanase$ production were optimized for ${\beta}-mannanase$ production. Locust bean gum of 10.0 g/l, soytone of 5.0 g/l, $KH_2PO_4$ of 2.0 g/l, NaCl of 10.0 g/l, $MgSO_4{\cdot}7H_2O\;of\;0.2\;g/l,\;Na_2CO_3$, of 2.0 g/l were selected as optimum content. Production of ${\beta}-mannanase$ by using the optimum medium was carried out. The maximum ${\beta}-mannanase$ activity of 20.8 unit/ml could be obtained after 14 h fermentation which corresponed to the productivity of ${\beta}-mannanase$ of 1.48 unit/ml-h.

• 한국미생물·생명공학회지
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• 제26권3호
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• pp.232-237
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• 1998

${eta}$-Mannan polymer 분해 효소를 다랑 생산하는 균주를 토양으로부터 분리하고 분리균의 형태적 내지는 생화학적 특성을 조사 분석하여 Sporolactobacillus sp. M201로 동정하였다. Sporolactobacillus sp. M20l 분리균은 locust bean gum을 탄소원으로 사용, 배양했을 때 세포외 ${eta}$-mannanase그리고 세포내 ${eta}$-mannosidase와 ${alpha}$-galactosidase을 다량 생산하는 것으로 확인되었다. 효소 생산 최적 배지와 최적 배양 조건은 ${eta}$-mannanase 생산에는 2.0% locust bean gum, 0.5% peptone, 0.2% KH$_2$PO$_4$, 80 mg/l MgSO$_4$ 및 8 mg/l ZnSO$_4$ 배지 (pH 6.0)와 37$^{\circ}C$이었고 그리고 ${eta}$-mannosidase 생산은 질소원으로 peptone 대신 0.5% yeast extract를 첨가한 상기 배지(pH 5.0)와 3$0^{\circ}C$에서 배양했을 때 가장 높았다. 이상의 각 효소의 최적 생산 조건에서 각각 30시간과 24시간 진탕배양 했을때 ${eta}$-mannanase(10.6 units/ml)와 ${eta}$-mannosidase(1.35 units/ml)의 최고 생산량을 나타내었다.

• 열처리공학회지
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• 제24권3호
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• pp.144-148
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• 2011

Change in damping capacity with strain amplitude was studied in Mg-Al-Si alloy in as-cast, solution-treated and aged states, respectively. The as-cast microstructure of the alloy is characterized by eutectic ${\beta}$($Mg_{17}Al_{12}$) phase and Chinese script type $Mg_2Si$ particles. The solution treatment dissolved the ${\beta}$ phase into the matrix, while the aging treatment resulted in the distribution of continuous and discontinuous type ${\beta}$ precipitates. The solution-treated microstructure showed better damping capacity than as-cast and aged microstructures both in strain-dependent and strain-independent damping regions. The decrease in second-phase particles which weakens the strong pinning points on dislocations and distribution of solute atoms in the matrix, would be responsible for the enhanced damping capacity after solution treatment.

• 한국약용작물학회지
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• 제3권1호
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• pp.21-24
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• 1995

생열귀나무의 열매를 약용 또는 건강식품으로서 의 실용화 가능성을 검토하기 위한 목적의 일환으로 과실을 대원형(大圓形), 타원형(楕圓形) 및 소원형(小圓形)으로 분류하여 ascorbic acid와 ${\beta}-carotene$의 함량을 분석한 결과를 요약하면 다음과 같다. 1. Ascorbic acid의 함량은 대원형(大圓形)에서 건중(乾重) 100g 당(當) 911mg으로 가장 높았으며 타원형(楕圓形) 358mg, 소원형(小圓形) 344mg으로서 비교적 그 함량이 낮았다. 2. ${\beta}$-carotene의 함량은 타원형(楕圓形)에서 건중(乾重) 100g 당(當) 286mg으로 가장 높았으며 대원형(大圓形)도 208mg으로 비교적 높은 편이었으나 소원형(小圓形)은 24mg으로 매우 낮게 나타났다. 3. 종자(種子)의 ascorbic acid는 과육(果肉)보다 1/10 - 1/30의 수준이었으며 과육의 조추출물은 대원형(大圓形)과 소원형(小圓形)은 각각 2,600mg 및 2,700mg 이었으나 타원형(楕圓形)은 1,060mg로 낮은 함량을 나타내었다.

• 한국조리학회지
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• 제24권1호
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• pp.63-72
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• 2018

The aim of this study was to investigate bioactive compounds from three domestic tomato varieties (Rafito, Momotaro TY Winner, and Medison). Lycopene, ${\beta}-carotene$ and polyphenols were quantified and identified using HPLC and LC-MS/MS. The levels of lycopene ranged from 28.36 mg/100 g to 60.18 mg/100 g. The content of ${\beta}-Carotene$ ranged from 2.00 mg/100 g to 2.92 mg/100 g. Ten kinds of polyphenol compounds were identified: caffeic acid-hexose isomer (I), caffeic acid-hexose isomer (II), 3-caffeoylquinic acid, 5-caffeoylquinic acid, caffeoylquinic acid isomer, quercetin-3-apiosylrutinoside, quercetin-3-rutinoside, di-caffeoylquinic acid, tri-caffeoylquinic acid, and naringenin chalcone. The level of 5-caffeoylquinic acid was the highest in domestic tomato varieties, ranging from 12.71 mg/100 g to 28.40 mg/100 g. The content of quercetin-3-rutinoside ranged from 3.74 mg/100 g to 17.64 mg/100 g. The contents of 3-caffeoylquinic acid and quercetin-3-apiosylrutinoside were 1.01~2.31 mg/100 g and 5.84~6.83 mg/100 g, respectively. Arrestingly naringenin chalcone was found only in Medison variety (36.82 mg/100 g). These results revealed that domestic tomato can be a good source of bioactive compounds for human health.