• 한국잡초학회지
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• 제9권2호
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• pp.149-153
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• 1989

Allelopathy 작용성(作用性)을 나타내는 4종(種)의 잡초(雜草) 중에 존재(存在)하는 휘발성(揮發性) terpene 물질(物質)을 GC 및 GC-MS로 동정(同定)하였다. 쑥에서는 26종(種)의 휘발성(揮發性) terpene 류(類)를 분리(分離) 동정(同定)하였는데 이들은 monoterpene 16종(種)과 sesquiterpene 10종(種)이었다. 또한 토끼풀에서는 4종(種), 쇠비름과 냉이에서 각(各) 3종(種)이 확인(確認)되었다. 동정(同定)된 terpene 중 allelopathy 작용성(作用性)을 나타내는 물질(物質)로는 쑥에서 ${\alpha}$-phellandrene, 1,8-cineole, limonene, ${\beta}$-pinene, borneol, selinene 및 caryophyllene, 토끼풀에서 ${\alpha}$-pinene과 ${\beta}$-caryophyllene, 그리고 냉이에서 ${\alpha}$-pinene 등이었고, 쇠비름 중에는 allelopathy 작용성(作用性)을 나타내는 휘발성(揮發性) terpene 류(類)는 없는 것으로 나타났다.

• 한국약용작물학회지
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• 제12권2호
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• pp.102-107
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• 2004

This study was carried out to establish an optimum method for identifying the volatile components of Magnolia ovobata Thunb. using the dynamic headspace (Purge & Trap) and simultaneous distillation and extraction (SDE) method. Between the two different identification analysis, the volatile components were more easily detected in the SDE than the Purge & Trap method. Among the identified volatile components, the 12 compounds were detected to have similar retention times and match quality within the 45 minutes in both identification methods. The maximum values of the major volatile components were detected differently by SDE and (Purge & Trap) method such as ${\alpha}-pinene$ (3.4, 18.2%), ${\beta}-pinene$ (3.5, 10.3%), l-limonene (5.2, 15.4%). These results indicated that the Dynamic Headspace (Purge & Trap) was much more reliable method for identifying the volatile components of Magnolia ovobata Thunb. as compared to the SDE method.

• Journal of the Korean Wood Science and Technology
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• 제22권1호
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• pp.72-79
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• 1994

To analyze terpene components, the essentail oil were extracted with steam distillation method from normal needle, damaged needle and pinecone of Pinus densiflora. The extracted essential oil was analyzed by gas chromatography and gas chromatography-mass spectroscopy. The results were summarized as follows; 1. Normal needles involve 43 kinds of terpene components, but damaged needles contained only 29 kinds. The most abundant components of normal and damaged needles were respectable ${\alpha}$-pinene and caryophyllene oxide. ${\alpha}$-pinene content in normal meedles amounted to 15.99 percents and caryophyllene oxide in damaged was 8.15 percents. 2. Pinecone showed 23 kinds of terpene components and among them the most abundant component was ${\beta}$-phellandrene, of which content showed 19.31 percents. 3. In normal needles, excluding ${\alpha}$-pinene, the contents of 8 kinds of other monoterpenes, reached to 48 percents of the total terpenes, 4. In damaged needles, excluding ${\alpha}$-pinene, the contents of 4 kinds of other monoterpenes, reached to 11 percents of the total terpenes. 5. In pinecone, excluding camphene, the contents of 6 kinds of other monoterpenes, reached to 58 percents of the total terpenes.

• Applied Biological Chemistry
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• 제51권3호
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• pp.233-237
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• 2008

우리나라의 자생식물인 털진달래(Rhododendon mucronulatum Turcz. var. ciliatum Nakai)지상부위로부터 정유를 얻고, 휘발성분을 headspace(HS)와 solid-phase microextraction(SPME)법으로 흡착시킨 다음 GC-MS로 분석하였다. 털진달래 정유에는 탄화수소 15종, 알코올 3종, 아세테이트 1종 등 총 19종의 화학성분이 함유되어 있었으며, 다함량 성분은 2-${\beta}$-pinene(16.1%), camphene(11.9%), ${\delta}$-3-carene(11.4%), d,l-limonene(9.5%), and ${\gamma}$-terpinene(9.5%)이었다. SPME법으로 흡착된 시료에는 탄화수소 13종, 알코올 1종, 질소화합물 1종 등 총 17종의 휘발성 화학물질이 함유되어 있었고, 이들 중 다함량 성분은 camphene(19.6%), 2-${\beta}$-pinene(18.0%), ${\delta}$-3-carene(17.4%), timethyl hydrazine(9.7%), ${\gamma}$-terpinene(8.5%), and d,l-limonene(5.5%)이었다. 그리고 HS법으로 흡착된 시료에는 탄화수소 11종, 알코올 1종, 질소화합물 1종 등 총 13종의 휘발성 화학물질이 함유되어 있었으며, 이들 중 다함량 성분은 camphene(25.8%), ${\delta}$-3-carene(24.8%), 2-${\beta}$-pinene(20.2%), d,l-limonene(5.4%), tricyclene(5.1%), trimethyl hydrazine(4.6%)이었다. 털진달래 정유의 향취는 coniferous, balsamic, woody하였으며, HaCaT 각질형성세포에 대한 $IC_{50}$ 값은 0.030 ${\mu}g/mg$이었다.

• 원예과학기술지
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• 제28권4호
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• pp.696-700
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• 2010

국내외에서 유통되고 있는 로즈마리 정유 31점을 수집하여 GC통한 유효성분분석과 항산화 활력시험을 실시하였다. 주요 성분은 ${\alpha}$-pinene, camphene, ${\beta}$-pinene, 1.8-cineol, verbenone 및 borneol 등이 동정되었다. 주요성분에 따라 생산국별로 함량의 차이가 나타났으며, 프랑스 원산 정유의 품질이 우수하였고, 국내에서 재배하여 추출한 정유가 ${\alpha}$-pinene과 camphene의 함량이 높았다. ISO가 제시한 9가지 성분의 함량을 충족하는 제품은 총 31점 중 5점(16%)으로 낮았다. 전자공여능(EDA)은 4.8-96.0%로 현저한 차이를 보였으며, 60%이상의 항산화능 활력을 갖는 정유는 13%였다. 특정 성분 함량에 따른 항산화 활력정도의 차이는 일치하지 않았으며, 스위스 원산의 정유에서 가장 높은 항산화 활력을 보였다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 봄 학술발표회 발표논문집
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• pp.334-335
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• 2003

우리나라에서 자생하는 소나무속의 침엽수 중 가장 대표적인 수종은 소나무이며, 리기다소나무가 그 뒤를 잇는다. 이 두 수종을 대상으로 테르펜의 배출속도와 물질별 구성비율을 비교하여 본 결과, 소나무와 리기다의 유사점은 $\alpha$-pinene, myrcene, $\beta$-phellandrene이 주요하게 나타났다. 리기다소나무의 경우에는 소나무에서 보다 $\beta$-pinene이 다소 크게 나타났으나, 지역별로는 테르펜의 구성비 차이가 거의 없었다. 삼림으로부터 방출되는 테르펜의 배출특성을 연구하기 위해서는 보다 많은 수종에 대한 광범위한 연구가 필요할 것으로 사료된다.

• 약학회지
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• 제54권4호
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• pp.215-225
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• 2010

To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity, silica-induced intracellular $H_2O_2$ and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is $\alpha$-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is $\beta$-pinene (22.4%) in GC/MS assay. Other essential oils of PN were $\beta$-pinene, camphene, myrcene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene and PT were $\alpha$-pinene, camphene, phellandrene, limonene, terpinolene, $\alpha$-terpineol, bornyl acetate, $\alpha$-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular $H_2O_2$ and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and $\alpha$-pinene have DPPH radical and superoxide scavenging activities, $\beta$-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular $H_2O_2$ and hydroxyl radical generations. And also phellandrene and $\beta$-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.

• Journal of Ginseng Research
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• 제32권4호
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• pp.305-310
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• 2008

수삼을 소재로 하여 인공타액을 이용한 새로운 향료를 개발하고자 6년근 수삼으로부터 신속하게 분석 가능한 SPME 흡착법을 이용하여 GC/MS(HP 6890/5973)로 분석하였다. 그 결과 수삼에서 약 20개의 peak가 동정되었는데, 주로 hydrocarbon 인 ${\alpha}$-pinene, ${\beta}$-pinene, myrcene, limonene, ${\beta}$-panasinsene, ${\beta}$-elemene, ${\alpha}$-gurjunene, trans-caryophyllene, ${\alpha}$-gurjunene, ${\alpha}$-panasinsene, ${\alpha}$-neoclovene, trans-${\beta}$-farnasene, ${\alpha}$-humulene, ${\beta}$-neoclovene, ${\beta}$-selinene, ${\alpha}$-selinene, bicyclogermacrene 등 이었고 5종의 unknown peak가 존재하였다. 수삼의 향기성분 추출과정에 있어서 인공타액처리에 의한 수삼향기성분의 변화를 조사한 결과 수삼과 인공타액 처리분에 대한 주요 성분의 면적함량과 total면적을 비교하였을 때 20분경과 시에는 약 1%의 증가를 보였고, 40분경과 시에는 약 10%의 증가를 보였으며, 80분경과 시에는 24%의 감소를 보였다. 따라서 수삼은 40분 인공타액처리를 하는 것이 가장 수삼향의 강도를 높였다.

• 한국환경과학회지
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• 제19권5호
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• pp.627-637
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• 2010

Emission rate of monoterpene and isoprene was measured in five commonly growing tree species of conifers(Pinus thunbergii, Abies koreana, Cryptomeria japonica, Pinus densiflora, Chamaecyparis obtusa) at the Halla mountain sites. Dynamic flow enclosure technique was used and gas samples were collected into Tenax tube. The highest and lowest hourly emission rate was observed in Abies koreana (1.86 ${\mu}g$/gdw/hr) and Chamaecyparis obtusa (0.52 ${\mu}g$/gdw/hr), respectively. The major species of monoterpene from pine trees were ${\alpha}$-pinene, ${\beta}$-pinene, ${\beta}$-phellandrene, myrcene. Particularly, d-limonene was abundant in Abies koreana but ${\alpha}$-pinene, $d^3$-carene and sabinene was in Cryptomeria japonica. Emission rates of isoprene show less significant than those of monoterpene. And also seasonal emission rates of monoterpene were dependent on environmental factors such as temperature.

• 한국자원식물학회지
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• 제13권2호
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• pp.89-94
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• 2000

Cephalotaxus koreana Nakai is endemic species of Korea. Since old days, the fruits have been used as a parasiticide and leaves have been used for a pierced wound by insects. The essential oil components from its aerial parts and stem bark by steam distillation were investigated. Several compounds were characterized by GC-Mass spectra. It was found that the leaves, stembark and flowers contain the compounds of essential oils, 1-octene-3-ol, hexadecanoic acid; $\alpha$-pinene, $\Delta^3$-carene mainly, linalylacetate, $\beta$-cubebene, 3,4-octadine-7-methyl , ferruginol(stem bark) and $\alpha$-pinene mainly, $\beta$-pinene , cyclopropane-1,1-dimethyl-2-(3-methyl-1,3-butadienyl), etc. This Cephalotaxus spp. contains the first components of ferruginol(M.W.286.03, $C_{20}H_{30}O)$ which belong to diterpene.